EWM27385.1, cds3289 (CDS) Nannochloropsis gaditana

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|585109612|gb|EWM27385.1| (putative inactive purple acid phosphatase 27 [Nannochloropsis gaditana])

HSP 1 Score: 1566.21 bits (4054), Expect = 0.000e+0
Identity = 754/754 (100.00%), Postives = 754/754 (100.00%), Query Frame = 0

Query:    1 MTGVETAMLQPLFVMNTTYSKKRPVQLCSGYLSGRIHGGISGARHVCHPSQGRHLFYALRFLVLLMSLPVTNAHGLGPRRARIHAFSPEAVVMNDTAFRSVSGALSRERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLRGKDTASWERKRREGGTESPVPG 754
            MTGVETAMLQPLFVMNTTYSKKRPVQLCSGYLSGRIHGGISGARHVCHPSQGRHLFYALRFLVLLMSLPVTNAHGLGPRRARIHAFSPEAVVMNDTAFRSVSGALSRERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLRGKDTASWERKRREGGTESPVPG
Sbjct:    1 MTGVETAMLQPLFVMNTTYSKKRPVQLCSGYLSGRIHGGISGARHVCHPSQGRHLFYALRFLVLLMSLPVTNAHGLGPRRARIHAFSPEAVVMNDTAFRSVSGALSRERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLRGKDTASWERKRREGGTESPVPG 754          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|761910720|ref|XP_011404695.1| (PREDICTED: probable inactive purple acid phosphatase 27 [Amphimedon queenslandica])

HSP 1 Score: 359.762 bits (922), Expect = 1.054e-111
Identity = 223/631 (35.34%), Postives = 326/631 (51.66%), Query Frame = 0

Query:  108 ERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPAD----VNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLD--IGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLR 732
            + +E  ++    V   +TP+ L  SG+   V    V +P   TD++ VYAP +    ++    AP+K ++  E        S++ S+G+     RL+N+R  Y FAL++G   AP L+  S  + F   NE  Q HLALT     + ++W +RD+K+P V++W                                + T +   TQ                        A +  Y+  D+C PPAT++GY DPG LHT  ++GL  G  Y Y+ GD     WS+  +F+ P   S   +        +F  FGDMG A+ D +L       +  ++N T L+   ++        LVLHIGD+SYA GY   WDEF    Q I   VP+MV  GNHERD         S S + G DSGGECGVP ++RF MP P    +  + WYDFS G VH+V++ TE DF+  S Q++++ D LS+ V+R++TPW++ A HRP+YIDSD+  G   SSD  V++ +Q ++E ++++YKVDL  +GHHHSYQRTC V +  C    +     APVHVV+GMAG  LS N+   QP     VD   +G T ++V+   LT+ ++     G   DTF+L+
Sbjct:   27 KYEEAVVNTDPSVVITVTPNQLNKSGDWVTVAWDGVSHPS-DTDWIGVYAPPNGEESIDPSKIAPVKYQYCNES-------STHMSSGKGSFKIRLVNVRTPYMFALLKGGFDAPSLVATSKQVTFSSPNEPLQPHLALTSDPTTLLLTWNTRDSKEPKVKFW--------------------------------QNTTTNIRTQA-----------------------ATSNKYTSKDMCGPPATTVGYIDPGMLHTAKLSGLTPGQEYNYQFGDDP--EWSQVFSFRMPPAPSPNAS-------ISFIAFGDMGQAQVDDTLRPLYVHAQPPAVNNTNLMAKEVNER-----DLVLHIGDISYAIGYAGVWDEFFDLIQPISSRVPYMVCGGNHERDYPH------SGSYYEGTDSGGECGVPYEMRFQMPRP----DPKQHWYDFSLGSVHFVLMSTEIDFTVNSVQYNWLKDHLSS-VDRSVTPWLIFAGHRPMYIDSDY--GLLPSSDLVVSKELQDNIEPLLLEYKVDLAFWGHHHSYQRTCPVAKKVCQDDGT-----APVHVVIGMAGHSLSTNIQNKQPDWIRFVDVDDYGYTRISVSPLSLTLEYIK--SDGTTKDTFTLK 560          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|302842359|ref|XP_002952723.1| (hypothetical protein VOLCADRAFT_105635 [Volvox carteri f. nagariensis] >gi|300262067|gb|EFJ46276.1| hypothetical protein VOLCADRAFT_105635 [Volvox carteri f. nagariensis])

HSP 1 Score: 359.377 bits (921), Expect = 2.588e-110
Identity = 234/627 (37.32%), Postives = 319/627 (50.88%), Query Frame = 0

Query:  147 DPR-TDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLG-----------------FQEANEVTQIHLALTGIDGEMAVSWVSRDT-KKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNV-WSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYI---DSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLRGKDTASWERKRREGGTES 750
            DPR  D++A+ AP+D NL  TAP K KF   +         +  TG   L FRL++ R    FALMR     P  + +S P+                      NE  Q+HLALTG   EM V W +R+    P V+W    P+    D+  G   AG  DG                                   P    ++ A T+ Y   DLC   ATS G+ D G  H  ++TGL+    Y YRVGD D +  WS   +F +         E    ET       DMG AE DGSL+ G E + SLNTTR ++   + S   P SL+LHIGD+SYA+GY  QWD F+ Q + +   +P+MV+ GNHERD         S   F  EDSGGECGV  + RFPMP P     KD+ WY F+ GP+ +++  TE    PGS Q+ F++ AL   V+R  TPW+VVA HRP+Y+   +++W DG     DQ V+ L++ +LED+ +++ VD+ L GHHHSYQRTC +YR  C  ++ D    APVHVV+G AG GLS N++   P+  E +    +G   M V+R+ L +  + D + G  +D+F LR    A+W +K  E  T S
Sbjct:  119 DPRYDDWIALVAPSDANLSETAPAKWKFAAGDPK-------HVITGSGSLRFRLISYRADVAFALMRNGFDTPQEVARSQPIKRSLSSKPCSSAGAVTVRLLNPNEPLQVHLALTGSPSEMRVQWNTREAGSTPQVRW---GPKSVKYDDRDGLGFAGGSDG-----------------------------------PAYPSTAAADTSRYGIEDLCGGAATSAGWVDAGHHHVALLTGLRPATRYYYRVGDPDGDGGWSPEFSFLS-------SPEISPDETVHILAVADMGQAEVDGSLE-GSEMIPSLNTTRRMIEEAAAS---PYSLLLHIGDISYARGYSTQWDNFMHQIEPLAARMPYMVAPGNHERDWPG------SGDFFGVEDSGGECGVAYERRFPMPYP----GKDKQWYAFAYGPIFFILYSTEHPVGPGSEQYEFIVQALRG-VDRRRTPWLVVAGHRPIYVASTNANWPDG-----DQPVSELLRDALEDLFLEHAVDMTLQGHHHSYQRTCPLYRGVCQPSNDDGTAAAPVHVVLGHAGAGLSLNIVDPLPAWLENL-GLWWGYVRMKVSRSQLLVEVVGD-DDGHFMDSFELR--KPANWGKKYMEVHTRS 669          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|470456063|ref|XP_004341026.1| (diphosphonucleotide phosphatase 1, putative [Acanthamoeba castellanii str. Neff] >gi|440797888|gb|ELR18962.1| diphosphonucleotide phosphatase 1, putative [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 353.214 bits (905), Expect = 3.131e-109
Identity = 216/612 (35.29%), Postives = 304/612 (49.67%), Query Frame = 0

Query:  120 VRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSL 731
            +    TP+ L+ SGE   V  + V +P    D++ VY+PA+ ++  + PIK KF  E        ++Y STG   + FRL N+R  Y F   R  ++ P L+  S+ + F   NE  Q  L LTG   EM                                            V W +++              +   K G  P     S  A T+TY +  +C  PA + G+ DPG LH+ V++ L+    Y Y  GD           F A   F                 FGDMG   +D S +    E+AS+NTT L+   L      P+ L+LHIGD++YA GY  QWDEF  Q  AI   +P+M   GNHERD  +      S S + G DSGGECGV  + R+PMP P R    D+PWY F  G +H+  + TE DFS GS Q+ ++ + L  KV+R  TPWVV + HRP+YIDS  D G+ A  DQ VAR ++ ++ED++ +Y+VDL L+GHHHSYQR+C VY+  C+ +      +AP HVV+GMAG  L+ N+   +P+    V+D+  G T + V R+ L + F+SD +   V D F+L
Sbjct:   49 ISIGATPATLQRSGEWVVVSWRGVDSPSA-GDWIGVYSPANASVTTSVPIKYKFADES-------TNYLSTGAGSVRFRLTNMRADYAFHFFRHGITRPTLVATSNAVTFVNYNEPMQGRLMLTGRQNEM-------------------------------------------RVMWTTRD------------AVRPQVKFGTSPGNYDQSVGAATSTYRKEHMCGAPANAEGWRDPGLLHSAVLSNLRPDTRYYYVYGDPTFG-------FSAEASFVSEPHPGQSDRVIHLFAFGDMGKTTQDNSTEHWDSELASINTTTLIAKDLDAR---PMDLLLHIGDIAYAVGYGAQWDEFHDQVSAISTRLPYMTCIGNHERDFPN------SGSRYNGSDSGGECGVAYEARYPMPTPAR----DQPWYSFDYGFIHFTFMSTEHDFSIGSVQWKWLEEDL-KKVDRVKTPWVVFSGHRPMYIDSQGDIGDAA--DQPVARELRANVEDLLFKYQVDLALWGHHHSYQRSCPVYKGTCIPSG-----RAPTHVVIGMAGFSLTTNLELEKPTWARVVNDQEHGYTRLAVTRSRLEMEFISDVD-TRVKDHFAL 568          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|340377913|ref|XP_003387473.1| (PREDICTED: probable inactive purple acid phosphatase 27 [Amphimedon queenslandica])

HSP 1 Score: 351.288 bits (900), Expect = 1.770e-108
Identity = 222/631 (35.18%), Postives = 323/631 (51.19%), Query Frame = 0

Query:  108 ERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPAD----VNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLD--IGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLR 732
            + +E  ++    V   +TP+ L  SG+   V    V +P   TD++ VYAP +    ++    AP+K ++  E        S++ S+G+     RL+N+R  Y FAL+ G  +AP L+  S  + F   NE  Q HLALT     + ++W +RD+ +P V++                              W +  T  R E                          A +  Y+  D+C PPAT++GY DPG LHT  ++GL  G  Y Y+ GD     WS+  +F+ P   S   +        TF  FGDMG A+ D +L       E  ++N T L+   ++        LVLHIGD+SYA GY   WDEF    Q I   VP+MV  GNHERD         S S + G DSGGECGVP ++RF MP P    +  + WY FS G VH+V++ TE DF+  S Q++++ D LS+ V+R++TPW++ A HRP+YIDS    G  A+SD  V++ +Q ++E ++++YKVDL  +GHHHSYQRTC V +  C    +     APVHVV+GMAGQ LS N+   QP     VD   +G T ++V+   LT+ ++     G   +TF+L+
Sbjct:   27 KYEEAVVNTDPSVVITVTPNQLNKSGDWVTVAWDGVSHP-ADTDWIGVYAPPNGEESIDPSKIAPVKYQYCKES-------STHMSSGKGSFKIRLVNVRTPYVFALLTGGFNAPTLVATSKQVTFSSPNEPLQPHLALTNDPTTLLLTWSTRDSHEPKVKF------------------------------WQNMTTYIRIEA-------------------------ATSNKYTSKDMCGPPATTVGYIDPGMLHTAKLSGLTPGQEYNYQFGDDP--EWSQVFSFRMPPAPSPNAS-------ITFIAFGDMGQAQVDDTLQPLYVHAEPPAVNNTNLMAKEVNER-----DLVLHIGDISYAIGYAGVWDEFFDLIQPISSRVPYMVCGGNHERDYPH------SGSYYEGTDSGGECGVPYEMRFQMPRP----DPKQHWYGFSLGSVHFVLMSTEIDFTVNSVQYNWLKDHLSS-VDRSVTPWLIFAGHRPMYIDST--AGVQAASDLVVSKELQDNIEPLLLEYKVDLAFWGHHHSYQRTCPVAKKVCQDDGT-----APVHVVIGMAGQSLSGNIQEKQPDWIRFVDVDDYGYTRISVSPLSLTLEYIK--SDGTQKETFTLK 560          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|470323646|ref|XP_004349546.1| (hypothetical protein CAOG_02793 [Capsaspora owczarzaki ATCC 30864] >gi|765551075|gb|KJE91696.1| hypothetical protein CAOG_002793 [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 334.339 bits (856), Expect = 1.188e-101
Identity = 214/644 (33.23%), Postives = 317/644 (49.22%), Query Frame = 0

Query:  105 LSRERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPG--PWRE--------LEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLA------ASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLR 732
            L+  R+    ++   + F +TP+ LE +     V    + +P P  D++A+Y P   NL A  P+K K          +  ++ S+G   L+F L+N+RDS +F   RG ++AP+ + Q+ P+ F+  +     HLA+T    EM                                           S+ W S+            K A   A  G        +  A TT+YS  D+C  PATS GY   G +HT + TGL+    Y Y  GD    + +      AP          G      + VFGDMG AE DGS +    E  S+NTT  ++  L       +  V H GD+SYA+GY   WD F +Q + I   VP++++SGNHERD ++      S +LF G DSGGECGVP   RF MPG  P  +        + KD PWY  + GP+H  V+ TE DFS GS Q +++   L++ V+R++TPW++ A HRP+YIDS   D    + DQ VA  ++Q +E ++ +Y+ DL ++GHHHSYQR+C      C+       A++   Y  PV+VV+GMAGQ LSQN++  QPS    V+D+ +G   +  ++T L   F+ +    ++ D F+LR
Sbjct:   34 LAAMRRATKAESPRSLAFAVTPNTLEAAEGTVTVIWAGLDDPQP-DDWIALYTPLPSNLSAIVPVKFKMC-------TISPTHLSSGSGSLTFTLINMRDSNSFVFFRGGLTAPVAVAQTDPVEFESYDIPMHPHLAITDNPSEM-------------------------------------------SLMWTSR------------KAAMPIALLGTSTTSVTTTFNATTTSYSASDMCGEPATSYGYRPAGLIHTVIFTGLQPRTRYYYVFGDPSYGMSTIYSFVSAPA--------RGDTSLVRWVVFGDMGRAERDGSNEYQVYEPPSINTTDRIIAELK---RGDVDFVGHFGDISYARGYASDWDSFFAQVRPIASAVPYLIASGNHERDWNN------SGALFPGYDSGGECGVPYNARFLMPGSKPTSKAGVRMDGGIVKDSPWYSANYGPIHLTVMSTEHDFSAGSTQLAWIEQDLAS-VDRSVTPWLLFAGHRPMYIDS--TDVSPVTGDQPVATALRQFVEPLLFKYRADLTMFGHHHSYQRSCPSLNLTCITTPQPPNAATPWSYLGPVNVVIGMAGQSLSQNLIAAQPSWVVAVNDQVYGYARLQADKTSLAFQFIIN-NSDQIGDQFTLR 593          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|470456042|ref|XP_004341024.1| (Ser/Thr phosphatase family superfamily protein [Acanthamoeba castellanii str. Neff] >gi|440797886|gb|ELR18960.1| Ser/Thr phosphatase family superfamily protein [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 330.102 bits (845), Expect = 2.175e-100
Identity = 220/627 (35.09%), Postives = 300/627 (47.85%), Query Frame = 0

Query:  118 GEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAP--GYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHS-----------YQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSL 731
            G+VR +  P  L+ SGE   V +          D+V VY+PAD ++  TAPIK K+  E K            G   L FRL N+R  Y F    G +  P L+  S+ + F   NE  Q  L LTG+  EM                                            V W +  T S               K G  P     S  A TTTY+R  +C  PA + G+ DPG  H+ V++ L     Y Y  GD       E     AP  G  SR         T     +GDMG   +  +      E AS+NTTRL++  +      P+ L +HIGD+SYA GY  QWDEF  Q  AI   +P+M   GNHERD  +      S S F G DSGGECGV  ++R+PMP P R    D+PWY F  G VH+V + +E +F+ G  Q+ + I+A   KV+RT TPW++ + HRP+YIDS++D G+  S+DQ VAR ++++LED++ +Y+VDL  +GHHHS           YQR+C V+   C++       +A  HVV+GMAG  LS ++    PS    VD    G T ++V  + L +TF+SD +   V D F L
Sbjct:   39 GDVRMSAWPGELKRSGE--WVSVSWSGASASWGDWVGVYSPADADVTVTAPIKYKYADEFKD-----------GYGKLWFRLTNMRADYVFHYFSGGIDKPTLIASSNRVTFANYNEPLQGRLMLTGVPHEM-------------------------------------------RVMWTTLNTTS------------PQVKFGTSPGQYVGSVSASTTTYTRDQMCGAPANTEGWRDPGLFHSAVLSNLSPDTRYYYVYGDPAYGFSEEASFMSAPRPGAASR---------TLNIFAYGDMGKTTQHWN-----NEKASINTTRLMIKDMQAI---PMDLAIHIGDISYAVGYGAQWDEFHDQVSAISTRLPYMTCIGNHERDFPN------SGSRFNGTDSGGECGVAYEVRYPMPTPGR----DQPWYSFDYGSVHFVFMSSEHNFTIGGTQWQW-IEADLRKVDRTKTPWIIFSGHRPMYIDSNYDKGD--SADQPVARELRRNLEDLLFKYRVDLAFWGHHHSSVESCLLVGAQYQRSCPVFNGTCMSEG-----QATTHVVIGMAGYRLSTDIPLTMPSWARVVDVSENGYTRLSVTSSRLEMTFISDVD-ARVKDHFVL 561          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|470454802|ref|XP_004340876.1| (Ser/Thr phosphatase family protein [Acanthamoeba castellanii str. Neff] >gi|440797743|gb|ELR18820.1| Ser/Thr phosphatase family protein [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 324.324 bits (830), Expect = 4.344e-98
Identity = 222/637 (34.85%), Postives = 311/637 (48.82%), Query Frame = 0

Query:  120 VRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKV--------VPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHS-----------------YQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSL 731
            VR + +PS L  +GE  +V  + V +P  + D+V VY+PAD ++ +TAP+K +          + + Y  TG   L FRL+N+R SY F  MR   + P+L+  S+ + F   NE TQ  + LTG   EM V W + +  +P V++                   G   G     A AS  T  R     +L GA                                PA + G+ DPG LH+ V+TGL+    Y Y  GD       E   + A   F      E +  + T   FGDMG   +D S +    E AS NTTRL+M  ++     P  L+LHIGD++YA GY  QWDEF   + A  +V        +P+M   GNHERD  +      S S +TG DSGGECGVP + RFPMP P R    D+PWY F  G VH+  + TE D+S GS Q+ ++ + L  +VNR+ TPWV+ + HRP+YI +         ++   AR M++ LEDV+ ++KVDL L+GH+HS                 YQR+C VY+  C+            HVV+GM G  L Q V  + PS    V++K  G T + +  + L + F+SD +GG V D FSL
Sbjct:   40 VRVSASPSALRHTGEWVEVSFEGVGSPH-KGDWVGVYSPADADVHSTAPVKWQHAD-------VSAEYLRTGAGKLRFRLINMRASYVFHFMRNGTAHPVLVSSSNHVTFANYNEPTQGRIMLTGRPSEMRVMWTTLNASRPAVRF-------------------GTATGQLTLTAAASSSTYHR----EQLCGA--------------------------------PANADGWRDPGLLHSAVLTGLRPDTRYYYVYGD-------EAYGWSAERSFVSGPTAEQRDRSLTLFAFGDMGKTTQDDSKEHWNLEGASRNTTRLMMEDMAAQ---PRDLLLHIGDIAYAVGYSAQWDEFHDMSAAGGRVQVEPLATQLPYMTCIGNHERDFPN------SGSYYTGSDSGGECGVPYEARFPMPTPAR----DQPWYSFDYGFVHFTFMSTEHDYSIGSKQWLWLEEDL-RRVNRSATPWVIFSGHRPMYIST--------KTESHSARHMRKELEDVLHKHKVDLALWGHNHSLTSVAYFPSIMVKTKHQYQRSCPVYKETCVPEGHGV-----THVVIGMGGFRLGQ-VGDHDPSWARVVNNKENGYTRLHITPSELDMQFVSDIDGG-VKDHFSL 577          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|552818476|ref|XP_005845202.1| (hypothetical protein CHLNCDRAFT_137433 [Chlorella variabilis] >gi|307104848|gb|EFN53100.1| hypothetical protein CHLNCDRAFT_137433 [Chlorella variabilis])

HSP 1 Score: 321.242 bits (822), Expect = 1.632e-96
Identity = 207/595 (34.79%), Postives = 300/595 (50.42%), Query Frame = 0

Query:  151 DFVAVYAPADVNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEGGEVLDTFSLRGKDTASWERKRRE 745
            D++A++ PA  +   TAPIK  +     S  +L       G   ++FRLLN+R    FAL+R  +  P+++  S  +   E N+  Q HL+LTG  GE+ V WV+RD   P V+                               W ++             GA   + AG             + TY+R D+C  PA + G+ DPG LH  VM GL+   +Y Y+ GD +L  WS   +F +P          G   +       D+G AE DGS+     E + +  +     AL+  +     L++H GD+SYA+G+  QWD +  Q    ++ VP+M + GNHERD       H         DSGGECGVP   R  MP P     +D+PWY F  GP+H+    TE  F PGS Q  F+   L+  V+R++TPWVVV  HRP+YIDS +  G     DQ VA+ ++ SLED++ +Y+VD    GHHHSYQRTC VYR +CL A++D   +AP+H+V+G AG GL+ N+   +P IF+ V  +H G  ++  N TH++   L+  + G +LD F+L     A W  + R+
Sbjct:   81 DYIALF-PAAGDPLITAPIKYHWAARSPSHLIL-------GTGSVTFRLLNMRQDMRFALVRSGLQFPVVVAWSGVVTVAEPNQPMQGHLSLTGKPGEVKVQWVTRDAGSPAVR-------------------------------WGTR------------SGAHEWSAAGD------------SLTYTRADMCGAPANASGWVDPGWLHGAVMAGLQPSTTYFYQYGDEELG-WSGEESFVSP-------PATGPGASVRLLAVADLGQAEVDGSM-----ESSEMLPSLATTAALAAEVQAGAQLLVHNGDISYARGFGSQWDTYFDQLGPTVRRVPYMTTVGNHERDWP-----HSGDRFPAQYDSGGECGVPYYRRTRMPTP----AEDKPWYSFDFGPIHFCQFSTEHLFEPGSEQHRFIERDLA-AVDRSVTPWVVVGGHRPIYIDSTFY-GLMPDGDQYVAKKLRDSLEDLLYRYQVDATWTGHHHSYQRTCAVYRGRCLGANADGTARAPLHLVIGHAGAGLTPNIHFFRPRIFDTVRLQH-GYVVVEANATHMSHRVLASYD-GSLLDEFTL--TKPAGWRSQPRD 584          

BLAST of EWM27385.1 vs. NCBI_GenBank
Match: gi|340373727|ref|XP_003385391.1| (PREDICTED: probable inactive purple acid phosphatase 27 [Amphimedon queenslandica])

HSP 1 Score: 317.39 bits (812), Expect = 3.648e-95
Identity = 189/602 (31.40%), Postives = 302/602 (50.17%), Query Frame = 0

Query:  124 ITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPAD----VNLQATAPIKIKFVGEEKSPPLLPSSYASTGEACLSFRLLNLRDSYTFALMRGNVSAPLLLGQSSPLGFQEANEVTQIHLALTGIDGEMAVSWVSRDTKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKVETFTFAVFGDMGTAEEDGSLDIGGEEVASLNTTRLLMGALSPSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDSDWDDGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAVYKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAE 721
            + P+ + +S +   V +  V +P+   +++ ++  AD    +N  + AP+K +++  +       + Y ++G A L F  +N+R  Y F    G + +P+L+  S  +     N+  Q HLALT    ++ + WV+++T  PL                               V W    T SR                       Q++  A  + Y+  D+C  PA   G+ DPG +HT  M  L     Y Y+ G      WS+  TFK+P          G         +GD+G    D +L I   E ASLNTT+ +   ++ +      L++HIGDLSYA G+  QWDE+ ++ + +    P+MV +GNHE D  + +      S F  +DSGGEC +P   R  MP     +   +PWY F  G VH+V++ +E +F+ G+ Q+ F++  L+  VNRT TPW+V   HRP+Y+DS     E+    Q +A+L++ +LED+++QY V L L+GHHH+YQRTC+VYR++C             HV++GMAG+ L Q+  PN+PS FE +DD+H+G T +  N T LT+ ++ + +
Sbjct:   47 VQPATINSSYQWFNVQVSGVSSPN-EDNWIGLFTLADNETEINATSHAPVKFQYLNVD-------TGYLTSGNAQLDFYAINMRHDYMFGFFTGGLDSPVLMSTSERIVNLNPNQPLQGHLALTLEIDKIVLQWVTKNTTDPL-------------------------------VRWG---TESRNY---------------------QYTKQANNSKYTVNDMCGSPANDYGWMDPGTIHTVTMDNLSPSTRYYYQFGSNTWG-WSDEFTFKSP-------PVTGPDTPVRIITYGDLGHGVPDNTLQIKKLEQASLNTTKNVYSEINET-----ELIVHIGDLSYAVGFSAQWDEYYNEVEKLAANSPYMVCAGNHEADWPNTT------SYFQSKDSGGECNIPYIYRNQMP----RVSPVKPWYGFDFGCVHFVIMNSEDNFTMGTEQYRFLVQHLA-SVNRTATPWLVFTGHRPMYVDST--SIEEPYGMQPIAKLLRNNLEDLLIQYNVSLALWGHHHTYQRTCKVYRSQCTD-------NGITHVIIGMAGRPLLQDFEPNRPSYFEYLDDQHYGYTRLQANSTTLTLQYIRNDD 552