EWM27417.1, cds3259 (CDS) Nannochloropsis gaditana

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|585109652|gb|EWM27417.1| (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Nannochloropsis gaditana])

HSP 1 Score: 2459.49 bits (6373), Expect = 0.000e+0
Identity = 1190/1190 (100.00%), Postives = 1190/1190 (100.00%), Query Frame = 0

Query:    1 MIEEGSEGRGTVDGGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYGLPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTNPFLLSDLRTELAVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDETV 1190
            MIEEGSEGRGTVDGGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYGLPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTNPFLLSDLRTELAVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDETV
Sbjct:    1 MIEEGSEGRGTVDGGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYGLPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTNPFLLSDLRTELAVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDETV 1190          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|553185831|ref|XP_005854316.1| (pyrophosphate--fructose-6-phosphate 1-phosphotransferase, partial [Nannochloropsis gaditana CCMP526] >gi|422294740|gb|EKU22040.1| pyrophosphate--fructose-6-phosphate 1-phosphotransferase, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 2172.51 bits (5628), Expect = 0.000e+0
Identity = 1058/1089 (97.15%), Postives = 1059/1089 (97.25%), Query Frame = 0

Query:  102 GARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYGLPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTNPFLLSDLRTELAVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDETV 1190
            GA+RVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDT                              RSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYGLPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTNPFLLSDLRTELAVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDETV
Sbjct:    1 GAKRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTVSSVPR------------------------RSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYGLPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTNPFLLSDLRTELAVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDETV 1065          

HSP 2 Score: 338.961 bits (868), Expect = 2.639e-95
Identity = 191/497 (38.43%), Postives = 276/497 (55.53%), Query Frame = 0

Query:   14 GGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFA---GARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLM--AAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCG-PVEDWKCGGVPLTMMMNMEK-------RHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYE---FLPEEEVVDA 494
            G +P+ VGVV  G QA GGHNVIAG++DY+ +   +S LLGF  G  G++  +   + +  ++ YRN GG D LG    K   P+  A   G +  C  L LDGLV +GG  + T  A L+EY  A G  T     P  IDG L N ++  + GF TACK YSEL+GN  +D  S++KY+ F+RLMG + S+IALE AL++ PN  L++E+V+A   SL +V   +  ++  RA + +HYG +L+PEG++  IPE   L+ E++     +     + EAV+  L+  + A+   +P  I+ Q+   R     VQ++ IETE+LL   +  EL + +  GQY G F P     GY+ R  LPS FD N  YS+G  A  L+  G T  + +ITG+ G   EDWK  GVPLT M+  ++        +G+ +P +    V L G  +K L  +R  W + D Y   GP+QF G  + D   T+  E   +L E EV+ A
Sbjct:  534 GERPITVGVVFCGRQAPGGHNVIAGLHDYLAA--SNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKF--PDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHA 1026          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|298710116|emb|CBJ31829.1| (RecName: Full=Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta; Short=PFP; AltName: Full=6-phosphofructokinase, pyrophosphate dependent; AltName: Full=Pyrophosphate-dependent 6-ph [Ectocarpus siliculosus])

HSP 1 Score: 653.67 bits (1685), Expect = 0.000e+0
Identity = 323/568 (56.87%), Postives = 405/568 (71.30%), Query Frame = 0

Query:   16 QPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEG---KPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEE---------EVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYG 571
            +PL +GVVLSGGQA GGHNVIAGV+D+ K   PDS++ GF NGP GV  G    I    ++AYRNTGGFD LGSGR+KIE+ E+   +++V + L LDGL+VIGGDDSNTNAA+LAEYF ++GC T+V G PKTIDGDLKN+Y+P+SFGFDTA KT+SE IGNV LD L+SQKYYHF+RLMGR+AS+IALECALQTRPN  LISEEVEA+ LSL Q+   LV MIL+RA  GK+YGV++LPEG+IEFIPEF  L+ ++ND++A+G+  T E V+  L   NR VF+YLP +IK QLLLDRDPHGNVQVAKIETE LLA  +  ELEK+   G+YA  F PQFHSFGYEGR  LPS FD +YCY+LG TAG L   G+ +LM     +  P+EDW  GGVP+TMM +ME+R GK KPVI KALV+L+G   +PF+     R+KW   D YRAPG IQF    +++ N+TL YE L E+             AP+ P +M   + +LF P  +A  S V+  RL+Y+ +VP  LK R+   V++   E +Q     DR  + R FP +YG
Sbjct:   81 KPLNMGVVLSGGQAPGGHNVIAGVFDFAKKCSPDSKVFGFLNGPAGVMKGICTEITPELMDAYRNTGGFDMLGSGRDKIETKEQMEASKKVAEELNLDGLIVIGGDDSNTNAAILAEYFESSGCKTKVNGCPKTIDGDLKNEYVPISFGFDTAAKTFSEEIGNVQLDALASQKYYHFIRLMGRAASNIALECALQTRPNVCLISEEVEAKGLSLKQIVDELVEMILKRADAGKNYGVVMLPEGLIEFIPEFNALISDINDVLASGIPTTIEGVVPALQPANRNVFNYLPQSIKMQLLLDRDPHGNVQVAKIETEVLLAQAVEAELEKLAAEGKYAAKFAPQFHSFGYEGRCALPSHFDCSYCYALGYTAGALTSKGMNALMACANNLDMPIEDWTVGGVPITMMCHMERRKGKDKPVIMKALVELDGPRSQPFQAFAAEREKWMEHDMYRAPGAIQFYSEGARNANMTLMYELLGEKAKALTQCDLSETAAPE-PVAMGASK-FLFRPATKATRSHVERARLDYEPKVPGCLKSRSLEGVELEADERSQSSKQADRGPLERMFPSTYG 646          

HSP 2 Score: 488.804 bits (1257), Expect = 1.396e-149
Identity = 252/485 (51.96%), Postives = 334/485 (68.87%), Query Frame = 0

Query:  706 YKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAF-----RESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSM 1185
            ++Q TV+VT ELL  YRNQGG DLLGRS +    T A +   K+AC  L LDGLV +GG  TAT  AYLSEY    G+ T+   VP  I G + N FVE+ +GF TA KVYS+LVGNTAIDGASA+KYW F+RLMG + S+IALEVA+++ PN++LLAEDV A R SLQ++VRE++D+V AR+ + +++GT+++PEGLV +IPE+ VL++EID  +      ES     S E V  RLT WS ALL S+P +IQ ++   R S+  VQLS IETE++L  FVE EL +RK  G YKG+F+ VCS LGYQAR +LPSNFDC+  +++GGA A+L+H   +GYLAT+TGLK    E WK  GVPLT++L  D  +Q +L   GQ RPRVP   ++LRG AY  L          D+Y N GP+QF G TAD+R  ++  E  DYL +L  L  ALR ++ +CRPGCSS +L L+TK++  LT+++ +
Sbjct:  663 FEQSTVEVTDELLDAYRNQGGFDLLGRSRDSI-RTEAELAKAKEACAALDLDGLVVIGGNTTATDAAYLSEYFHTAGLKTSVACVPCTISGGMKNNFVESAVGFDTAVKVYSQLVGNTAIDGASARKYWYFMRLMGQDPSHIALEVAMQTHPNMVLLAEDVRAKRWSLQDIVREISDVVQARSDKGKNFGTIVIPEGLVAAIPEMEVLIEEIDALYVEHAGPESSSNTLSREEVQERLTVWSKALLASLPAFIQEELCLERQSNHRVQLSQIETEKMLVCFVEEELRKRKEAGSYKGNFSAVCSHLGYQARSALPSNFDCDYAFTLGGAVAILMHRRMSGYLATVTGLKE-PVEGWKGGGVPLTSLLGAD--DQEKLP-TGQCRPRVPPTPINLRGDAYLSLQKVLANGQRKDLYRNPGPVQFAGDTADNRLMSLELETDDYLGQLAELRGALRGIQESCRPGCSSNILKLSTKTLQNLTEIMEL 1142          

HSP 3 Score: 322.013 bits (824), Expect = 1.074e-88
Identity = 178/484 (36.78%), Postives = 267/484 (55.17%), Query Frame = 0

Query:  660 ERPITVGVVFCGRQAPGGHNVIAGLHDYL--AASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDL---RGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEE 1138
            ++P+ +GVV  G QAPGGHNVIAG+ D+    + +S + GF+ G  G+ K    ++T EL+  YRN GG D+LG   +K  +T   ME  K     L LDGL+ +GG  + T  A L+EY  + G  T     P  IDG L N++V  + GF TA K +SE +GN  +D  +++KY+ F+RLMG   SNIALE AL++ PN+ L++E+V+A   SL+++V E+ +++  RA   ++YG V++PEGL+  IPE   L+ +I+          T++E VV  L   +  +   +P  I++Q+   R     VQ++ IETE LL   VE EL +  A G+Y   F P     GY+ R +LPS+FDC+  Y++G  A  L   G    +A    L  +  EDW V GVP+T M        H     G+ +P +    V+L   R   ++    +R+ W  HDMY   G +QF+   A +   T++ E
Sbjct:   80 DKPLNMGVVLSGGQAPGGHNVIAGVFDFAKKCSPDSKVFGFLNGPAGVMKGICTEITPELMDAYRNTGGFDMLGSGRDKI-ETKEQMEASKKVAEELNLDGLIVIGGDDSNTNAAILAEYFESSGCKTKVNGCPKTIDGDLKNEYVPISFGFDTAAKTFSEEIGNVQLDALASQKYYHFIRLMGRAASNIALECALQTRPNVCLISEEVEAKGLSLKQIVDELVEMILKRADAGKNYGVVMLPEGLIEFIPEFNALISDINDVLASGIP--TTIEGVVPALQPANRNVFNYLPQSIKMQLLLDRDPHGNVQVAKIETEVLLAQAVEAELEKLAAEGKYAAKFAPQFHSFGYEGRCALPSHFDCSYCYALGYTAGALTSKGMNALMACANNLD-MPIEDWTVGGVPITMMC-------HMERRKGKDKPVIMKALVELDGPRSQPFQAFAAEREKWMEHDMYRAPGAIQFYSEGARNANMTLMYE 552          

HSP 4 Score: 321.627 bits (823), Expect = 1.206e-88
Identity = 164/422 (38.86%), Postives = 258/422 (61.14%), Query Frame = 0

Query:   74 FINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMA--AGVANTEEAVMRE-----LSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRH----GKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYE 484
             ++AYRN GGFD LG  R+ I +  E A A+  C +L LDGLVVIGG+ + T+AA L+EYF   G  T V   P TI G +KN+++  + GFDTA K YS+L+GN  +D  S++KY++F+RLMG+  SHIALE A+QT PN  L++E+V A+  SL  +   +  ++  R+ +GK++G I++PEG++  IPE + L+ E++ L    AG  ++   + RE     L+  ++A+ + LPA I+E+L L+R  +  VQ+++IETEK+L   +  EL K ++ G Y G F+      GY+ R+ LPS FD +Y ++LG     L+   ++  + ++TG+  PVE WK GGVPLT ++  + +     G+ +P +    + L G  +  L+K       +D YR PGP+QF G  + +  ++L+ E
Sbjct:  674 LLDAYRNQGGFDLLGRSRDSIRTEAELAKAKEACAALDLDGLVVIGGNTTATDAAYLSEYFHTAGLKTSVACVPCTISGGMKNNFVESAVGFDTAVKVYSQLVGNTAIDGASARKYWYFMRLMGQDPSHIALEVAMQTHPNMVLLAEDVRAKRWSLQDIVREISDVVQARSDKGKNFGTIVIPEGLVAAIPEMEVLIEEIDALYVEHAGPESSSNTLSREEVQERLTVWSKALLASLPAFIQEELCLERQSNHRVQLSQIETEKMLVCFVEEELRKRKEAGSYKGNFSAVCSHLGYQARSALPSNFDCDYAFTLGGAVAILMHRRMSGYLATVTGLKEPVEGWKGGGVPLTSLLGADDQEKLPTGQCRPRVPPTPINLRGDAYLSLQKVLANGQRKDLYRNPGPVQFAGDTADNRLMSLELE 1095          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|1206244584|ref|XP_021338244.1| (6-phosphofructokinase [Babesia microti strain RI] >gi|1139689601|emb|SJK86047.1| 6-phosphofructokinase [Babesia microti strain RI])

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 407/1134 (35.89%), Postives = 598/1134 (52.73%), Query Frame = 0

Query:   18 LRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLL----AGTIALE-LEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHG-----KFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEFLPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLH---RLEYKNRVPQALKGRTTLNVQVVEGEATQCLYGKDRALMRREFPRSYG-LPMVHLHADPKEGSQQRQVNAHKFSVSSNGTCAAEEEEIPRQGTTN-PFLLSDLRTEL-AVSEVDGMRSLLDREEPHASKRAATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGF---VGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLT-----NQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLR----PRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQF 1123
            +++G++LSGG A GGH VI+G +DY+K  +PDS+L+GF  G  G    KY  I +  I+ YRN GGFD L SGR +I++ ++   A ++   L LDGL++IGGD SN+NAA+LA YF        V+G PKTIDGD+K++ I  SFGFDTA +TYSELIGN+ +D  S++  +HFVR+MGRSASH+ALECA+QT PN  L+ EE++    SL ++ H +  ++++R K GK++GVIL+ EG+IEFIPE + L+ ELN+++  G    +E  +  L+  +R  + +LP  I+EQLLLDR+  G + VAKI TE+LL       IA E  + +++       F P  H FGYEGR  +PS FD+ YCYSLG  A  LI +     M  I  +    E+W   G+P   +M+M          KF P I++ LV L  K FK + K R  WA+ + YR+PGP+Q       ++N T   +F          DVP S+ Q     F     + LS+  +H    L+Y +      + +  +N  + +      L   D           Y  L  + L+  P      +  N       SNG     +    RQ   N P L S    E+  VS +D   + L    P ASK               VG+V   +Q PG  NVI G+ + L   N  L  F    G I+G+Y      +  E L  +RNQGGL ++ RS  K    ++      + C++L L  LV +G     +     +EYL++  +      VP      L      N  +E  +GF T  K+Y+ LVGN   D AS  KYW F++++G   S   LEVAL++ PN++++AE+  A+ K+L +VVR++AD V  RA   +++GTVL+P+ L+  +P +  L+ E+   + + +     V   +S+L++W+ AL  S P YI+  +     S   + L  +E E LL + V+ ELN RK  G YKG++  V  + GYQ R SLP+NFD NLG++ G  A V V +  TG    I GL   + E W++  VP   +L          + N   R    P V    V+L G +++ L   R  W   D++ N GP+Q+
Sbjct:  163 IKIGLILSGGPAPGGHCVISGAFDYLKIRNPDSKLIGFIGGIDGFLNNKYEDISSEKIDNYRNMGGFDMLWSGRGRIKNDKDLETAAKIATDLELDGLIIIGGDGSNSNAALLANYFEKLPRKICVVGVPKTIDGDVKSENIEQSFGFDTASRTYSELIGNLCVDATSTRYTWHFVRVMGRSASHLALECAMQTHPNILLVGEEIQENGTSLEEIVHQITDLMIQRMKLGKNFGVILISEGLIEFIPEVKILIQELNEIVNRG----QEFDVNLLN-KSRKTWEFLPYMIQEQLLLDREASGYIMVAKIATERLLLMLVESYIASEKTDDLKRLNSLKLQFMP--HYFGYEGRCAVPSDFDATYCYSLGYNASLLISHDRNGYMSVIRNLAKHYEEWVPMGLPFVSIMHMVDAPAGSDLPKF-PAIKRVLVDLNSKMFKAISKARITWALSENYRSPGPLQLK---LSNLNATCLEKF---------SDVPNSIKQTTSLTFD---SSSLSNKLVHVSDNLDYMS------ERKDDVNELISQQRCFSLLNDLDLVCKESTNGTCYSPLQNMRLNYKPNIPPLCKNPNLTMTRNISNGNTIPTDTYTYRQILLNYPHLTSKTSFEIYNVSAIDTPNNSL----PKASK---------------VGIVCLSKQYPGIMNVIWGVRERL--KNCELYAFQGTAGLINGVYS----IIKDEDLKLFRNQGGLGIIYRSRFKSIYPLSDRIAAANTCIKLGLHSLVIMGDSGAISQATLFAEYLLSNNIDINIIGVPVTGSNCLASFGCDNNPIEACVGFDTNAKLYAGLVGNVLTDAASIPKYWHFVKILGRLPSLEVLEVALQTHPNVVIIAEEYGAANKTLFDVVRDIADAVCQRAEIGKNFGTVLIPDHLIFHLPSMKTLIDELRSLYEKIDPGDNLVNH-ISKLSSWNKALFESFPEYIRKVLYTFDPSE--ILLENMEIEILLANMVKEELNLRKKKGLYKGNYLSVTHYFGYQGRCSLPTNFDSNLGFAYGHIAGVAVESNVTGVCVGIQGLCTQKVEQWEMFAVPFVRLLRISPDRPEIFSLNDHPRKGELPLVLCSMVNLSGKSFRALKEARGRWVYEDLFCNPGPIQY 1239          

HSP 2 Score: 274.248 bits (700), Expect = 5.162e-72
Identity = 167/493 (33.87%), Postives = 261/493 (52.94%), Query Frame = 0

Query:  661 RPITVGVVFCGRQAPGGHNVIAGLHDYLAASN--STLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVP-TACTV-VPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKA-----LGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKK---VDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRD 1141
            R I +G++  G  APGGH VI+G  DYL   N  S L+GF+GGIDG    +   ++SE +  YRN GG D+L     +  +   +    K A   L LDGL+ +GG  + +  A L+ Y   E +P   C V VP  IDG + ++ +E + GF TA + YSEL+GN  +D  S +  W F+R+MG + S++ALE A+++ PN++L+ E++  +  SL+E+V ++ DL+  R    +++G +L+ EGL+  IPE+ +L+QE++      +E    V  +     TW       +P  IQ Q+   R +S  + ++ I TERLL   VE+ +   K      L   K  F P   + GY+ R ++PS+FD    YS+G  A++L+   R GY++ I  L    EE W   G+P  +++   +      A  G   P+ PA K   VDL    +K +   R  W + + Y + GPLQ   L   +  AT +E+  D
Sbjct:  161 RRIKIGLILSGGPAPGGHCVISGAFDYLKIRNPDSKLIGFIGGIDGFLNNKYEDISSEKIDNYRNMGGFDMLWSGRGRIKNDKDLETAAKIA-TDLELDGLIIIGGDGSNSNAALLANYF--EKLPRKICVVGVPKTIDGDVKSENIEQSFGFDTASRTYSELIGNLCVDATSTRYTWHFVRVMGRSASHLALECAMQTHPNILLVGEEIQENGTSLEEIVHQITDLMIQRMKLGKNFGVILISEGLIEFIPEVKILIQELNEIVNRGQE--FDVNLLNKSRKTWEF-----LPYMIQEQLLLDREASGYIMVAKIATERLLLMLVESYIASEKTDDLKRLNSLKLQFMP--HYFGYEGRCAVPSDFDATYCYSLGYNASLLISHDRNGYMSVIRNLAKHYEE-WVPMGLPFVSIMHMVD------APAGSDLPKFPAIKRVLVDLNSKMFKAISKARITWALSENYRSPGPLQ---LKLSNLNATCLEKFSD 631          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|1129202176|gb|OLQ05052.1| (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 [Symbiodinium microadriaticum])

HSP 1 Score: 600.127 bits (1546), Expect = 0.000e+0
Identity = 307/549 (55.92%), Postives = 385/549 (70.13%), Query Frame = 0

Query:   14 GGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLK-NDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMA-AGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEG---KPFKILKKRRDKWAVEDCYRAPGPIQFD---GPCSQDVNLTLQYEF---LPEEEVVDAPDVPRSMTQMEGYLFSPTPRACLSSVQLHRLEYKNRVPQALKGRTTLNVQVVEGEAT 551
            G  P++VGVVLSGGQA GGHNVIAG++D IKS H DS ++GF +GP G+FTG +  I    ++ +RNTGGFD LGSGR+KIES E+F  +  VC ++ LDGLVVIGGDDSNTN AVLAEYF ANGC T+V+GAPKTIDGDLK   ++PVSFGFDTACKTY+ L+GNV +D LS+QKYYH VRLMGRSAS+IALE AL TRPN  L+ EEV     SL  +T  +  MI  R++ GK YGVI+LPEG+IEFIPEF  L+ E+ND +A  GV  TEE V+  LS  N   F YLP+ I+ QLLLDRDPHGNVQVAKIETEKLLA T+  EL+  R  G Y G F+PQFH+FGYEGRAGLP+ FD++YCY+LG TA  L+  G T L+ S+  +  P ++W+CGGVP+T +  +E+R GK KPVI KALV+L+G   +PF+  K  R++  + D YR PGPIQFD      S+D+ +TLQ E    LP E   + P       +M  +LF P  +   S +Q  R   ++R+P++L       V V  GEAT
Sbjct:  111 GEVPVKVGVVLSGGQAPGGHNVIAGIFDGIKSWHKDSTMIGFLDGPHGIFTGNFVEITDEIMDGFRNTGGFDMLGSGRHKIESEEQFKDSMTVCSAIGLDGLVVIGGDDSNTNGAVLAEYFKANGCKTKVVGAPKTIDGDLKCPPHLPVSFGFDTACKTYATLVGNVAVDALSAQKYYHLVRLMGRSASNIALEVALLTRPNMCLLGEEVAEGKKSLKDITMDMADMIEARSRDGKDYGVIILPEGLIEFIPEFNALISEINDKLAQPGVEATEEGVLAVLSKENAECFRYLPSFIRAQLLLDRDPHGNVQVAKIETEKLLAATVHAELDNRRAKG-YKGNFSPQFHAFGYEGRAGLPTVFDASYCYALGYTASSLLVTGWTGLIASVKNLLAPCKEWQCGGVPVTSLCVIERRKGKDKPVIHKALVELQGALSQPFEFYKSIREQLRISDMYRNPGPIQFDMVGCEASKDIPMTLQLELGRTLPVEFPTEKPK------RMGNFLFLPHAKKLRSELQQWRSARQHRLPESLSAFNGNAVTVHRGEAT 652          

HSP 2 Score: 446.432 bits (1147), Expect = 4.429e-132
Identity = 242/544 (44.49%), Postives = 338/544 (62.13%), Query Frame = 0

Query:  653 TTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAAS-NSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNV-------SNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEEDRDYLEELEVLHAALRQVRRACRPGCSSALLHLATKSILTLTDVVSMMDE 1188
            T  R A      +GVVFCGRQAPG H+ + GL D L  +  + +LGFVGG  G Y++QTV++T EL   +R  GGL++LGR+ ++  +T    E    AC  L LDGLV VGG RT T  AYLSE+     V TA   VP GI+G++ N+FVE +LGF +  K  ++LVGNTAIDG+SA+KY+ FL+LM  +        S++ALEVAL + PNL+LL E+VD  R SL+E+V ++AD+VS RA+  +++GTVLV EGL+ +IPE   L+ E+     E+    ++VE V+ +LTTWSAAL +S+P +IQ Q+  AR S+  +QLS +ETERL+   VE EL  RK  G +KGSF+PVC FLGYQAR S+PS+FD +  Y++GG AA+L   G  GY+A ++ L     E W V GVP TAML   ++       + + RP +   KV+L   A++     R      ++YEN GP+Q  G +A  R    +     YL+EL+ L   L  +R  CRPGC    + +AT+S+ TL  ++  + E
Sbjct:  678 TEERTAPIENKRIGVVFCGRQAPGCHDFVCGLVDMLPPNFGNKVLGFVGGTHGFYEKQTVEITPELCDAFRGTGGLEMLGRTVDRI-NTKEESENAMKACQELNLDGLVLVGGARTNTDAAYLSEFFKTNKVKTAVVGVPCGIEGSMVNEFVEASLGFDSCAKAMAQLVGNTAIDGSSARKYYYFLKLMDGSTTGGKVPSSHVALEVALETKPNLLLLTEEVDDHRTSLRELVSDIADVVSERASAGKNFGTVLVAEGLLAAIPEFRTLITEL-----EALPMPSTVEKVLPKLTTWSAALFQSLPEFIQKQLLLARQSNAALQLSQLETERLMAWLVEDELKSRKKQGTFKGSFSPVCQFLGYQARCSVPSDFDSDYAYALGGCAAILTSRGHNGYMAVVSDL-AQPVERWHVGGVPFTAML---QVPPTMPKESFRPRPGIFPHKVELESGAFRKWKEVRASCAREELYENPGPIQLSGPSA-SRVTKTIATRFSYLQELKALQRHLDMLRERCRPGCDLRQVKVATQSLATLNSILDELAE 1210          

HSP 3 Score: 336.265 bits (861), Expect = 9.716e-93
Identity = 180/478 (37.66%), Postives = 277/478 (57.95%), Query Frame = 0

Query:   19 RVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRS-------ASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMN----MEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEF 485
            R+GVV  G QA G H+ + G+ D +      +++LGF  G  G +  +   I     +A+R TGG + LG   ++I + EE   A + CQ L LDGLV++GG  +NT+AA L+E+F  N   T V+G P  I+G + N+++  S GFD+  K  ++L+GN  +D  S++KYY+F++LM  S       +SH+ALE AL+T+PN  L++EEV+    SL ++   +  ++ ERA  GK++G +L+ EG++  IPEF+TL+ EL  L    + +T E V+ +L+  + A+F  LP  I++QLLL R  +  +Q++++ETE+L+A  +  EL+  +K G + G F+P     GY+ R  +PS FDS+Y Y+LG  A  L   G    M  ++ +  PVE W  GGVP T M+     M K   + +P I    V+LE   F+  K+ R   A E+ Y  PGPIQ  GP +  V  T+   F
Sbjct:  689 RIGVVFCGRQAPGCHDFVCGLVDMLPPNF-GNKVLGFVGGTHGFYEKQTVEITPELCDAFRGTGGLEMLGRTVDRINTKEESENAMKACQELNLDGLVLVGGARTNTDAAYLSEFFKTNKVKTAVVGVPCGIEGSMVNEFVEASLGFDSCAKAMAQLVGNTAIDGSSARKYYYFLKLMDGSTTGGKVPSSHVALEVALETKPNLLLLTEEVDDHRTSLRELVSDIADVVSERASAGKNFGTVLVAEGLLAAIPEFRTLITELEAL---PMPSTVEKVLPKLTTWSAALFQSLPEFIQKQLLLARQSNAALQLSQLETERLMAWLVEDELKSRKKQGTFKGSFSPVCQFLGYQARCSVPSDFDSDYAYALGGCAAILTSRGHNGYMAVVSDLAQPVERWHVGGVPFTAMLQVPPTMPKESFRPRPGIFPHKVELESGAFRKWKEVRASCAREELYENPGPIQLSGPSASRVTKTIATRF 1162          

HSP 4 Score: 330.102 bits (845), Expect = 1.396e-90
Identity = 187/474 (39.45%), Postives = 277/474 (58.44%), Query Frame = 0

Query:  659 GERPITVGVVFCGRQAPGGHNVIAGLHDYLAA--SNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDA---CVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLT-NQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGA---AYKFLMGKRQLWGMHDMYENSGPLQF 1123
            GE P+ VGVV  G QAPGGHNVIAG+ D + +   +ST++GF+ G  GI+    V++T E++  +RN GG D+LG    K    +   E FKD+   C  + LDGLV +GG  + T  A L+EY  A G  T     P  IDG L     +  + GF TACK Y+ LVGN A+D  SA+KY+  +RLMG + SNIALEVAL + PN+ LL E+V   +KSL+++  ++AD++ AR+   + YG +++PEGL+  IPE   L+ EI+    +   E T  E V++ L+  +A   R +P +I+ Q+   R     VQ++ IETE+LL   V  EL+ R+A G YKG+F+P     GY+ R  LP+ FD +  Y++G  A+ L+ TG TG +A++  L    +E W+  GVP+T++   +          G+ +P +    V+L+GA    ++F    R+   + DMY N GP+QF
Sbjct:  111 GEVPVKVGVVLSGGQAPGGHNVIAGIFDGIKSWHKDSTMIGFLDGPHGIFTGNFVEITDEIMDGFRNTGGFDMLGSGRHK----IESEEQFKDSMTVCSAIGLDGLVVIGGDDSNTNGAVLAEYFKANGCKTKVVGAPKTIDGDLKCPPHLPVSFGFDTACKTYATLVGNVAVDALSAQKYYHLVRLMGRSASNIALEVALLTRPNMCLLGEEVAEGKKSLKDITMDMADMIEARSRDGKDYGVIILPEGLIEFIPEFNALISEINDKLAQPGVEATE-EGVLAVLSKENAECFRYLPSFIRAQLLLDRDPHGNVQVAKIETEKLLAATVHAELDNRRAKG-YKGNFSPQFHAFGYEGRAGLPTVFDASYCYALGYTASSLLVTGWTGLIASVKNLLAPCKE-WQCGGVPVTSLCVIER-------RKGKDKPVIHKALVELQGALSQPFEFYKSIREQLRISDMYRNPGPIQF 570          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|855302729|ref|XP_012894521.1| (uncharacterized protein [Blastocystis hominis] >gi|855314513|ref|XP_012898542.1| uncharacterized protein [Blastocystis hominis] >gi|855316321|ref|XP_012899137.1| uncharacterized protein [Blastocystis hominis] >gi|300123221|emb|CBK24494.2| unnamed protein product [Blastocystis hominis] >gi|300123818|emb|CBK25089.2| unnamed protein product [Blastocystis hominis] >gi|300175162|emb|CBK20473.2| unnamed protein product [Blastocystis hominis])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 274/469 (58.42%), Postives = 351/469 (74.84%), Query Frame = 0

Query:   16 QPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYE 484
            +P+ +GVVLSGGQA GGHNVIAG+YD+ K V     + GF  GP+GVFTGKY  ID  F++ YRN GGFD +GSGR+KI+  ++F  + +VC  L LDGL+VIGGDDSNTNAA+LAEYF +    T VIG PKTIDGDLKNDYIPVSFGFDTACK YSELIGN+ +D LS+QKYYHFVRLMGRSASHI +ECA +T PN T I EE+  +  S+ Q+T  +V +I+ERAK GK+YGVILLPEG+IEFIPE  +L+ ELN+++AAG    ++ +   L   +R +F YLP ++++QL LDRD HGNV+V++IETEK +   +  EL+ ++  G Y+G FN Q H FGYEGR GLPS FD+NYCY+LG  AG LI  GL  +M +I+ +  PVE W  GGVP+TMMMN+E+R+GK +PVI+KALV+L+G P++   ++  KW + DCY  PGPIQF GP +  +N TL YE
Sbjct:   64 RPMNIGVVLSGGQAPGGHNVIAGIYDFTKKV--GGNMYGFRYGPRGVFTGKYVKIDDKFMDQYRNMGGFDMIGSGRDKIKGDDQFQASAKVCNDLELDGLIVIGGDDSNTNAALLAEYFQSIHHRTHVIGCPKTIDGDLKNDYIPVSFGFDTACKIYSELIGNICVDALSAQKYYHFVRLMGRSASHITMECAFETHPNYTFIGEEISQKKKSVKQITQEVVDLIVERAKLGKNYGVILLPEGLIEFIPEMGSLIEELNEVIAAG-HTAQDDIAAHLQPASRELFDYLPQSLRDQLTLDRDSHGNVKVSQIETEKFIVSCVQKELDALKAQGVYSGSFNAQCHFFGYEGRCGLPSCFDTNYCYALGYNAGVLIHEGLNGMMSTISNLDAPVEAWTAGGVPVTMMMNIERRNGKDEPVIKKALVELDGLPYRRFVEQAKKWRLGDCYSFPGPIQFTGPAANVINQTLAYE 529          

HSP 2 Score: 330.872 bits (847), Expect = 2.001e-97
Identity = 172/469 (36.67%), Postives = 261/469 (55.65%), Query Frame = 0

Query:  661 RPITVGVVFCGRQAPGGHNVIAGLHDYLAASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTAD 1129
            RP+ +GVV  G QAPGGHNVIAG++D+       + GF  G  G++  + V++  + +  YRN GG D++G   +K           K  C  L LDGL+ +GG  + T  A L+EY  +    T     P  IDG L N ++  + GF TACK+YSEL+GN  +D  SA+KY+ F+RLMG + S+I +E A  + PN   + E++   +KS++++ +EV DL+  RA   ++YG +L+PEGL+  IPE+  L++E++          T+ + + + L   S  L   +P  ++ Q+T  R S   V++S IETE+ +   V+ EL+  KA G Y GSF   C F GY+ R  LPS FD N  Y++G  A VL+H G  G ++TI+ L     E W   GVP+T M+    +E+     NG+  P +    V+L G  Y+  + + + W + D Y   GP+QF G  A+
Sbjct:   64 RPMNIGVVLSGGQAPGGHNVIAGIYDFTKKVGGNMYGFRYGPRGVFTGKYVKIDDKFMDQYRNMGGFDMIGSGRDKIKGDDQFQASAK-VCNDLELDGLIVIGGDDSNTNAALLAEYFQSIHHRTHVIGCPKTIDGDLKNDYIPVSFGFDTACKIYSELIGNICVDALSAQKYYHFVRLMGRSASHITMECAFETHPNYTFIGEEISQKKKSVKQITQEVVDLIVERAKLGKNYGVILLPEGLIEFIPEMGSLIEELNEVIAAGH---TAQDDIAAHLQPASRELFDYLPQSLRDQLTLDRDSHGNVKVSQIETEKFIVSCVQKELDALKAQGVYSGSFNAQCHFFGYEGRCGLPSCFDTNYCYALGYNAGVLIHEGLNGMMSTISNLDA-PVEAWTAGGVPVTMMM---NIERR----NGKDEPVIKKALVELDGLPYRRFVEQAKKWRLGDCYSFPGPIQFTGPAAN 520          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|470448430|ref|XP_004340159.1| (diphosphate-fructose-6-phosphate 1-phosphotransferase [Acanthamoeba castellanii str. Neff] >gi|440797044|gb|ELR18139.1| diphosphate-fructose-6-phosphate 1-phosphotransferase [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 577.785 bits (1488), Expect = 0.000e+0
Identity = 283/488 (57.99%), Postives = 356/488 (72.95%), Query Frame = 0

Query:   17 PLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALG-----------------SGR-NKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDY-IPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEF 485
            PLR+GVVLSGGQA+GGHNVIAG++DY+ + HP S+L GF  GP G+  G + VIDA  +  YRN GGFD +G                 S R +KIE+ ++   +R VC++LALDGLV+IGGDDSNTNAA+LAEYF ++   T V+G PKTIDGDLK+   + VSFGFDTAC  YSELIGN+  D  SS+KYYHF+RLMGRSASHI LECALQTRPN TLI EEV A   +LS +T+ L  +I+ERAK GK++G++LLPEG+IEF+PE   L+ ELN+LMA G  N+ + V  +L+  +RAVF +LP+ I+ +LLLDRDPHGNVQV+KIETEKL    +  EL++ +  GQ+ G F+   H FGYEGR GLPS FDSNYCY+LG TAG L+ +GLT  M S++ +  P E WK GG+PLT +MNME+R GK  PVIRKALV L+  P+K L + R+KWA  + YR PGPIQF GP S +VN+TLQ EF
Sbjct:   76 PLRIGVVLSGGQAAGGHNVIAGLFDYVAAGHPHSKLYGFIGGPIGLVKGDFIVIDAPLVAKYRNQGGFDIIGAIALHHSRVDQKNLLAESKRADKIETDKQLLASRVVCENLALDGLVIIGGDDSNTNAALLAEYFKSHHLKTNVVGVPKTIDGDLKSSLGVEVSFGFDTACMIYSELIGNIATDANSSRKYYHFIRLMGRSASHITLECALQTRPNITLIGEEVAARKQTLSDLTNELCHVIVERAKAGKNFGIVLLPEGLIEFVPEVHRLISELNELMAKG--NSADVVGEKLTEESRAVFDFLPSAIRHELLLDRDPHGNVQVSKIETEKLFIHLVTKELKRRKANGQFKGSFSAMGHFFGYEGRCGLPSNFDSNYCYALGRTAGALLDHGLTGYMASVSNLTAPPEQWKSGGLPLTALMNMERRKGKNVPVIRKALVDLDAAPYKELLRHREKWARGEHYRNPGPIQFYGPASGEVNITLQLEF 561          

HSP 2 Score: 343.199 bits (879), Expect = 1.374e-101
Identity = 195/495 (39.39%), Postives = 284/495 (57.37%), Query Frame = 0

Query:  651 AATTHRAAGERPITVGVVFCGRQAPGGHNVIAGLHDYLAASN--STLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRS--HEKFPDTVAMMEGFKDA---------------CVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQF-VETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHG 1125
            A+T    A   P+ +GVV  G QA GGHNVIAGL DY+AA +  S L GF+GG  G+ K   + + + L++ YRNQGG D++G    H    D   ++   K A               C  L LDGLV +GG  + T  A L+EY  +  + T    VP  IDG L +   VE + GF TAC +YSEL+GN A D  S++KY+ F+RLMG + S+I LE AL++ PN+ L+ E+V A +++L ++  E+  ++  RA   +++G VL+PEGL+  +PE+  L+ E++    E   +  S + V  +LT  S A+   +P  I+ ++   R     VQ+S IETE+L  H V  EL RRKA GQ+KGSF+ +  F GY+ R  LPSNFD N  Y++G  A  L+  G TGY+A+++ L    E+ WK  G+PLTA++    +E+ +    G+  P +    VDL  A YK L+  R+ W   + Y N GP+QF+G
Sbjct:   65 ASTAGDDASHSPLRIGVVLSGGQAAGGHNVIAGLFDYVAAGHPHSKLYGFIGGPIGLVKGDFIVIDAPLVAKYRNQGGFDIIGAIALHHSRVDQKNLLAESKRADKIETDKQLLASRVVCENLALDGLVIIGGDDSNTNAALLAEYFKSHHLKTNVVGVPKTIDGDLKSSLGVEVSFGFDTACMIYSELIGNIATDANSSRKYYHFIRLMGRSASHITLECALQTRPNITLIGEEVAARKQTLSDLTNELCHVIVERAKAGKNFGIVLLPEGLIEFVPEVHRLISELN----ELMAKGNSADVVGEKLTEESRAVFDFLPSAIRHELLLDRDPHGNVQVSKIETEKLFIHLVTKELKRRKANGQFKGSFSAMGHFFGYEGRCGLPSNFDSNYCYALGRTAGALLDHGLTGYMASVSNLTAPPEQ-WKSGGLPLTALM---NMERRK----GKNVPVIRKALVDLDAAPYKELLRHREKWARGEHYRNPGPIQFYG 547          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|1147556914|ref|XP_020111133.1| (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Ananas comosus])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 273/471 (57.96%), Postives = 342/471 (72.61%), Query Frame = 0

Query:   15 GQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEF 485
            G  L++GVVLSGGQA GGHNVI+G++DY++ +   S L GF  GP G+   KY  +   FI  YRN GGFD + SGR+KIE+PE+F  A      L LDGLVVIGGDDSNTNA +LAE F A    TRVIG PKTIDGDLK+  +P SFGFDTACK YSE+IGNVM D  S+ KYYHFVRLMGR+ASHI LECALQT PN TLI EEV A+  +L +VT  +  +I +RA+ G +YGVIL+PEG+I+FIPE Q L+ ELN+++A  V + E    ++L   +R +F +LP  I+EQLLL+RDPHGNVQVAKIETEK+L   +  ELEK R  G+YAG F  Q H FGYEGR GLP+ FD++YCY+LG  AG L+ +G T L+ S+  +C PVE+W  GG  LT +M++E+RHGKFKPVI+KA+V+LEG PF      RD+WA+++ Y +PGPIQF GP + DVN TL  E 
Sbjct:   89 GSALKIGVVLSGGQAPGGHNVISGIFDYLQEMAKGSVLYGFKGGPAGIMRCKYVELTTDFIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVSKLDLDGLVVIGGDDSNTNACLLAENFRAKSMKTRVIGCPKTIDGDLKSKEVPTSFGFDTACKIYSEMIGNVMTDARSTGKYYHFVRLMGRAASHITLECALQTHPNITLIGEEVAAKKQTLKEVTDYITDIICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDEEGVWKKKLREQSRFLFEFLPQAIQEQLLLERDPHGNVQVAKIETEKMLISMVETELEKRRSEGKYAGHFRGQSHFFGYEGRCGLPTNFDASYCYALGYAAGALLHSGKTGLISSVGNLCAPVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFIKFASMRDEWAIKNRYISPGPIQFHGPGANDVNHTLLLEL 559          

HSP 2 Score: 328.561 bits (841), Expect = 2.475e-96
Identity = 182/479 (38.00%), Postives = 275/479 (57.41%), Query Frame = 0

Query:  663 ITVGVVFCGRQAPGGHNVIAGLHDYLA--ASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVV-SRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHGLTADDRPATVVEE 1138
            + +GVV  G QAPGGHNVI+G+ DYL   A  S L GF GG  GI + + V++T++ +  YRNQGG D++    +K  +T    +  ++   +L LDGLV +GG  + T    L+E   A+ + T     P  IDG L ++ V T+ GF TACK+YSE++GN   D  S  KY+ F+RLMG   S+I LE AL++ PN+ L+ E+V A +++L+EV   + D++  RA    +YG +L+PEGL+  IPE+  L+ E++        ++   E V   +L   S  L   +P  IQ Q+   R     VQ++ IETE++L   VETEL +R++ G+Y G F     F GY+ R  LP+NFD +  Y++G AA  L+H+G+TG ++++  L    EE W V G  LTA++  D   +H     G+ +P +    V+L GA +      R  W + + Y + GP+QFHG  A+D   T++ E
Sbjct:   92 LKIGVVLSGGQAPGGHNVISGIFDYLQEMAKGSVLYGFKGGPAGIMRCKYVELTTDFIYPYRNQGGFDMICSGRDKI-ETPEQFKQAEETVSKLDLDGLVVIGGDDSNTNACLLAENFRAKSMKTRVIGCPKTIDGDLKSKEVPTSFGFDTACKIYSEMIGNVMTDARSTGKYYHFVRLMGRAASHITLECALQTHPNITLIGEEVAAKKQTLKEVTDYITDIICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEIL---AHDVVDEEGVWKKKLREQSRFLFEFLPQAIQEQLLLERDPHGNVQVAKIETEKMLISMVETELEKRRSEGKYAGHFRGQSHFFGYEGRCGLPTNFDASYCYALGYAAGALLHSGKTGLISSVGNLCAPVEE-WTVGGTALTALM--DVERRH-----GKFKPVIKKAMVELEGAPFIKFASMRDEWAIKNRYISPGPIQFHGPGANDVNHTLLLE 558          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|1029000899|ref|XP_016755195.1| (PREDICTED: pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta-like [Gossypium hirsutum])

HSP 1 Score: 565.844 bits (1457), Expect = 0.000e+0
Identity = 271/478 (56.69%), Postives = 345/478 (72.18%), Query Frame = 0

Query:    8 GRGTVDGGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEF 485
            G  ++   Q L++GVVLSGGQA GGHNVI G++DY++   P S L GF  GP GV  GKY  + A +I  YRN GGFD + SGR+KIE+PE+F  A      L LDGLVVIGGDDSNTNA +LAE F +    TRVIG PKTIDGDLK   +P SFGFDTACK Y+E+IGNVM+D  S+ KYYHFVRLMGR+ASHI LECALQT PN T+I EEV A+ L+L  VT  +V +I +RA+ G +YGVIL+PEG+I+FIPE Q L+ ELN+++A  V +      ++L+  +  +F +LP  I+EQL+L+RDPHGNVQVAKIETEK+L   +  ELEK ++ G Y   F  Q H FGYEGR GLP+ FD++YCY+LG  AG L+ +G T L+ S+  +C PVE+W  GG  LT MM++E+RHGKFKPVI+KA+V+L+G PFK     R++WA+++CY +PGPIQF GP S  VN TL  E 
Sbjct:   85 GIDSIGSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQERAPGSTLYGFRGGPAGVMKGKYVELTADYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETAAKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDENGLWKKKLTDQSLKLFEFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQEGSYKANFKGQSHFFGYEGRCGLPTNFDASYCYALGYGAGALLHSGKTGLISSVGNLCAPVEEWTVGGTALTSMMDVERRHGKFKPVIKKAMVELDGAPFKKFASLREEWAIKNCYISPGPIQFVGPASNAVNHTLLLEL 562          

HSP 2 Score: 320.472 bits (820), Expect = 2.895e-93
Identity = 175/468 (37.39%), Postives = 266/468 (56.84%), Query Frame = 0

Query:  660 ERPITVGVVFCGRQAPGGHNVIAGLHDYLA--ASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHG 1125
            ++ + +GVV  G QAPGGHNVI G+ DYL   A  STL GF GG  G+ K + V++T++ +  YRNQGG D++    +K  +T    +  ++   +L LDGLV +GG  + T    L+E   ++ + T     P  IDG L  + V T+ GF TACK+Y+E++GN  ID  S  KY+ F+RLMG   S+I LE AL++ PN+ ++ E+V A + +L+ V   + D++  RA    +YG +L+PEGL+  IPE+  L+ E++        +   +     +LT  S  L   +P  IQ Q+   R     VQ++ IETE++L   VETEL +RK  G YK +F     F GY+ R  LP+NFD +  Y++G  A  L+H+G+TG ++++  L    EE W V G  LT+M+  D   +H     G+ +P +    V+L GA +K     R+ W + + Y + GP+QF G
Sbjct:   92 DQKLKIGVVLSGGQAPGGHNVICGIFDYLQERAPGSTLYGFRGGPAGVMKGKYVELTADYIYPYRNQGGFDMICSGRDKI-ETPEQFKQAEETAAKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDENGLWK--KKLTDQSLKLFEFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQEGSYKANFKGQSHFFGYEGRCGLPTNFDASYCYALGYGAGALLHSGKTGLISSVGNLCAPVEE-WTVGGTALTSMM--DVERRH-----GKFKPVIKKAMVELDGAPFKKFASLREEWAIKNCYISPGPIQFVG 548          

BLAST of EWM27417.1 vs. NCBI_GenBank
Match: gi|823235576|ref|XP_012450426.1| (PREDICTED: pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Gossypium raimondii] >gi|763800314|gb|KJB67269.1| hypothetical protein B456_010G184300 [Gossypium raimondii])

HSP 1 Score: 565.844 bits (1457), Expect = 0.000e+0
Identity = 270/478 (56.49%), Postives = 344/478 (71.97%), Query Frame = 0

Query:    8 GRGTVDGGQPLRVGVVLSGGQASGGHNVIAGVYDYIKSVHPDSELLGFANGPKGVFTGKYYVIDAAFINAYRNTGGFDALGSGRNKIESPEEFAGARRVCQSLALDGLVVIGGDDSNTNAAVLAEYFAANGCSTRVIGAPKTIDGDLKNDYIPVSFGFDTACKTYSELIGNVMLDCLSSQKYYHFVRLMGRSASHIALECALQTRPNATLISEEVEAEHLSLSQVTHSLVRMILERAKQGKHYGVILLPEGIIEFIPEFQTLMHELNDLMAAGVANTEEAVMRELSFNNRAVFSYLPANIKEQLLLDRDPHGNVQVAKIETEKLLAGTIALELEKIRKYGQYAGVFNPQFHSFGYEGRAGLPSGFDSNYCYSLGLTAGHLIGNGLTSLMVSITGVCGPVEDWKCGGVPLTMMMNMEKRHGKFKPVIRKALVQLEGKPFKILKKRRDKWAVEDCYRAPGPIQFDGPCSQDVNLTLQYEF 485
            G   +   Q L++GVVLSGGQA GGHNVI+G++DY++   P S L GF  GP G+  GKY  + A +I  YRN GGFD + SGR+KIE+PE+F  A      L LDGLVVIGGDDSNTNA +LAE F +    TRVIG PKTIDGDLK   +P SFGFDTACK Y+E+IGNVM+D  S+ KYYHFVRLMGR+ASHI LECALQT PN T+I EEV A+ L+L  VT  +V +I +RA+ G +YGVIL+PEG+I+FIPE Q L+ ELN+++A  V +      ++L+  +  +F +LP  I+EQL+L+RDPHGNVQVAKIETEK+L   +  ELEK ++ G Y   F  Q H FGYEGR GLP+ FD++YCY+LG  AG L+ +G T L+ S+  +C PVE+W  GG  LT MM++E+RHGKFKPVI+KA+V+L+G PFK     R++WA ++CY +PGPIQF GP S  VN TL  E 
Sbjct:   85 GVDNIGSDQKLKIGVVLSGGQAPGGHNVISGIFDYLQERAPGSTLYGFKGGPAGIMKGKYVELTADYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETAAKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDENGLWKKKLTDQSLKLFEFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQEGSYKANFKGQSHFFGYEGRCGLPTNFDASYCYALGYGAGALLHSGKTGLISSVGNLCAPVEEWTVGGTALTSMMDVERRHGKFKPVIKKAMVELDGAPFKKFASLREEWASKNCYISPGPIQFVGPASNAVNHTLLLEL 562          

HSP 2 Score: 321.242 bits (822), Expect = 1.312e-93
Identity = 176/468 (37.61%), Postives = 266/468 (56.84%), Query Frame = 0

Query:  660 ERPITVGVVFCGRQAPGGHNVIAGLHDYLA--ASNSTLLGFVGGIDGIYKQQTVQVTSELLSTYRNQGGLDLLGRSHEKFPDTVAMMEGFKDACVRLRLDGLVFVGGCRTATGVAYLSEYLMAEGVPTACTVVPAGIDGTLTNQFVETTLGFHTACKVYSELVGNTAIDGASAKKYWMFLRLMGSNVSNIALEVALRSAPNLILLAEDVDASRKSLQEVVREVADLVSARAARNRHYGTVLVPEGLVLSIPEIAVLLQEIDVAFRESEEELTSVEAVVSRLTTWSAALLRSMPPYIQVQMTKARSSSKGVQLSAIETERLLCHFVETELNRRKALGQYKGSFTPVCSFLGYQARGSLPSNFDCNLGYSMGGAAAVLVHTGRTGYLATITGLKGVEEEDWKVAGVPLTAMLAYDELEQHRLAYNGQLRPRVPAKKVDLRGAAYKFLMGKRQLWGMHDMYENSGPLQFHG 1125
            ++ + +GVV  G QAPGGHNVI+G+ DYL   A  STL GF GG  GI K + V++T++ +  YRNQGG D++    +K  +T    +  ++   +L LDGLV +GG  + T    L+E   ++ + T     P  IDG L  + V T+ GF TACK+Y+E++GN  ID  S  KY+ F+RLMG   S+I LE AL++ PN+ ++ E+V A + +L+ V   + D++  RA    +YG +L+PEGL+  IPE+  L+ E++        +   +     +LT  S  L   +P  IQ Q+   R     VQ++ IETE++L   VETEL +RK  G YK +F     F GY+ R  LP+NFD +  Y++G  A  L+H+G+TG ++++  L    EE W V G  LT+M+  D   +H     G+ +P +    V+L GA +K     R+ W   + Y + GP+QF G
Sbjct:   92 DQKLKIGVVLSGGQAPGGHNVISGIFDYLQERAPGSTLYGFKGGPAGIMKGKYVELTADYIYPYRNQGGFDMICSGRDKI-ETPEQFKQAEETAAKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVDENGLWK--KKLTDQSLKLFEFLPQAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQEGSYKANFKGQSHFFGYEGRCGLPTNFDASYCYALGYGAGALLHSGKTGLISSVGNLCAPVEE-WTVGGTALTSMM--DVERRH-----GKFKPVIKKAMVELDGAPFKKFASLREEWASKNCYISPGPIQFVG 548