EWM27469.1, cds3207 (CDS) Nannochloropsis gaditana

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|585109729|gb|EWM27469.1| (thiamine biosynthesis protein [Nannochloropsis gaditana])

HSP 1 Score: 1337.78 bits (3461), Expect = 0.000e+0
Identity = 641/641 (100.00%), Postives = 641/641 (100.00%), Query Frame = 0

Query:    1 MPFITRRAALQAAAMAFLSGSCVEGWLVGSGVKATGKLTMRKIETSLAAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEFAKVGQVYTEETELAKKLGKE 641
            MPFITRRAALQAAAMAFLSGSCVEGWLVGSGVKATGKLTMRKIETSLAAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEFAKVGQVYTEETELAKKLGKE
Sbjct:    1 MPFITRRAALQAAAMAFLSGSCVEGWLVGSGVKATGKLTMRKIETSLAAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEFAKVGQVYTEETELAKKLGKE 641          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|1129207637|gb|OLQ09500.1| (Phosphomethylpyrimidine synthase, chloroplastic [Symbiodinium microadriaticum])

HSP 1 Score: 930.243 bits (2403), Expect = 0.000e+0
Identity = 431/559 (77.10%), Postives = 493/559 (88.19%), Query Frame = 0

Query:   73 SGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYG--VDEKVFEVGMENMSKEFAKVG-QVY 628
            SGRS+PT DPYNP+F     F+EAYP   K Y  V +E TGE + +PFRR+HL D DPGC+HLD+YDTSGP G +PR+GL KLRK W+  RE   D T TQM+YAKQG++T+EMA++AAREGMDP+FV SE+ARGRAIIP+N+ H E EPMI+GR+FKVK+NSNIGNSAV S+IEEEVEKLQWS+LWGADTLMDLSTGKHIH+TREWI+RN+ +PVGTVPIYQALEKVDGI E+LTWE FRETLIEQAEQGVDY+TIHAGVLLR++P+TA R+TGIVSRGGSIHAKLC+  H+ENFAYEHWDDILDIC KYD+SLSIGDGLRPG IKDANDEAQF EL  QGELT+RAW K VQVMNEGPGHVPLHKIPENMR QL+WC EAPFYTLGPLTTD+APGYDHITSAIGAA IG++GTA+LCYVTPKEHLGLP+R+DVKAG+IAYRIAAHAADLAK HP+AQEWDDALSK+RFEFRW DQFNLA+DPITAR+ HDETIDS+ GK+AHFCSMCGPKFCSMKIT+DVR YA + GYG    E V + GM+ MSK+FA+VG +VY
Sbjct:   64 SGRSRPTSDPYNPEFKDAFQFKEAYPSSEKIYKEVVYEATGEKLKIPFRRIHLTDEDPGCDHLDVYDTSGPPGADPRKGLAKLRKKWIEGREVAEDGTCTQMYYAKQGVITQEMAFIAAREGMDPEFVRSELARGRAIIPANRNHPESEPMIIGRHFKVKINSNIGNSAVASNIEEEVEKLQWSSLWGADTLMDLSTGKHIHETREWIVRNSAMPVGTVPIYQALEKVDGIAEDLTWEKFRETLIEQAEQGVDYWTIHAGVLLRFVPLTAKRLTGIVSRGGSIHAKLCISKHEENFAYEHWDDILDICKKYDVSLSIGDGLRPGCIKDANDEAQFGELKVQGELTQRAWAKGVQVMNEGPGHVPLHKIPENMRKQLEWCHEAPFYTLGPLTTDIAPGYDHITSAIGAANIGAMGTAMLCYVTPKEHLGLPDREDVKAGIIAYRIAAHAADLAKGHPKAQEWDDALSKARFEFRWNDQFNLALDPITARSMHDETIDSEQGKSAHFCSMCGPKFCSMKITDDVRKYAEERGYGDADGETVLKEGMKEMSKKFAEVGSEVY 622          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|298708972|emb|CBJ30925.1| (conserved unknown protein [Ectocarpus siliculosus])

HSP 1 Score: 929.087 bits (2400), Expect = 0.000e+0
Identity = 446/632 (70.57%), Postives = 505/632 (79.91%), Query Frame = 0

Query:    1 MPFITRRAALQAAAMAFLSGSCVEGWLVGSGVKATGKLTMRKIETSLAAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEFAKVG-QVYTEE 631
            MP    + A+ AA  + +     +G      +   G + +R     +A+     P   A G       +P GSGR K T DPYNPDF    +F +AYP+ TK Y  + H  TG  M VPFRR+HL+D DPGC HLD+YDT+GP G +PR+GLP +R  WV RRE  GD   TQM YAK+GI+TEEMAY AAREGMDP+FV SEV RGRAIIPSNKRH+ELEPMI+GR FKVKVNSNIGNS V SS+E EVEKLQWSTLWGADTLMDLSTG HI QTREWI+RN+PIPVGTVPIYQALEKVDGI E+LTWEV R+TLIEQAEQGVDYFTIHAGVLL ++P TA R+TGIVSRGGSIHAK C+  HKENFAYEHWD+ILDIC KYDISLSIGDGLRPG IKDAND AQFAEL  QGELT RAW K+VQVMNEGPGHVPLHKIPENMRNQLDWC EAPFYTLGPLTTD+APGYDHITSAIGAA IG++GTA+LCYVTPKEHLGLPN+ DVK GL+AYRIAAHAADLAK HPRAQEWDD LSK+RFEFRW DQFNLA+DP TAR+YHDET+ +D GK AHFCSMCGPKFCSMKIT+DVR YA++ GYGV+E     GME M + + + G Q+Y+E+
Sbjct:    1 MPLC--KVAMAAAIYSSVGSEAFQGGFFAKSLGKVGPVRVRSTHQMVASPPKVEPVEVAPGFN-----KP-GSGRVKRTTDPYNPDFHEVASFGDAYPESTKRYQEIVHAETGHKMKVPFRRIHLSDPDPGCSHLDVYDTAGPLGADPRKGLPSVRGEWVKRREDSGDEVFTQMHYAKKGIITEEMAYCAAREGMDPEFVRSEVERGRAIIPSNKRHVELEPMIIGRLFKVKVNSNIGNSDVVSSVEAEVEKLQWSTLWGADTLMDLSTGAHIRQTREWILRNSPIPVGTVPIYQALEKVDGIAEDLTWEVLRDTLIEQAEQGVDYFTIHAGVLLSFVPHTADRLTGIVSRGGSIHAKWCIAHHKENFAYEHWDEILDICNKYDISLSIGDGLRPGCIKDANDFAQFAELKVQGELTSRAWAKDVQVMNEGPGHVPLHKIPENMRNQLDWCHEAPFYTLGPLTTDIAPGYDHITSAIGAANIGAMGTAMLCYVTPKEHLGLPNKYDVKQGLMAYRIAAHAADLAKGHPRAQEWDDCLSKARFEFRWNDQFNLALDPPTARSYHDETLPADGGKTAHFCSMCGPKFCSMKITDDVREYAKEQGYGVEE-ALAAGMEKMGELYKQKGQQLYSED 623          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|1026777442|gb|OAE35940.1| (hypothetical protein AXG93_1278s1140 [Marchantia polymorpha subsp. ruderalis])

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 446/647 (68.93%), Postives = 514/647 (79.44%), Query Frame = 0

Query:    7 RAALQAAAMAFLSGSCVEGWLVGSGVKATGKLTMRKIETSLAAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEFAKV-------------GQVYTEETELAKKLGK 640
            RA+L ++       +CV G+     +    KL+++K   +   AT  +       +  +       S R++PTKDP +PDF+    FEE +P  T+E+  V HE T  V+ VPFRRVHL+  +P C   DLY TSGPQ +N R GLP LRKSW+ +RE +GD   TQM+YAKQGI+TEEM Y A RE ++P+FV SEVARGRAIIPSNKRHLELEPMIVGR F VKVN+NIGNSAVTSSIEEEV+KLQWST+WGADT+MDLSTG+HIH+TREWIMRN+P+PVGTVPIYQALEKVDGI ENLTWEVFRETLIEQAEQGVDYFTIHAGVLLR+IP+TA RMTGIVSRGGSIHAK CL  HKENFAYEHWDDILDIC KYDISLSIGDGLRPGSI DAND AQF+EL TQG LTRRAWEK+VQVMNEGPGH+PLHKIPENM  QLDWC+EAPFYTLGPLT D+APGYDHITSAIGAA IG+LGTALLCYVTPKEHLGLPNRDDVKAG+IAY+IAAHAADLAKQHP AQEWDDALSK+RFEFRW DQF L++DP+TA+++HDET+ ++  K AHFCSMCGPKFCSM ITEDVR YA +HGYG  ++  + GME MS EF  V             G++Y  E+ +A   GK
Sbjct:    9 RASLLSSGQ---KNACVPGFESLRALTGPTKLSLQKRRNAPRTATRNSSSRVNMTLTVDS---DKLSQRTRPTKDPASPDFLPIPPFEECFPNSTREFKEVVHEPTNTVLQVPFRRVHLSGGEPPC---DLYLTSGPQNLNARAGLPHLRKSWIEKREARGDERCTQMYYAKQGIITEEMLYCAVRENLEPEFVRSEVARGRAIIPSNKRHLELEPMIVGRKFLVKVNANIGNSAVTSSIEEEVQKLQWSTMWGADTIMDLSTGRHIHETREWIMRNSPVPVGTVPIYQALEKVDGIAENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRFIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNKYDISLSIGDGLRPGSIYDANDAAQFSELATQGVLTRRAWEKDVQVMNEGPGHIPLHKIPENMEKQLDWCNEAPFYTLGPLTIDIAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQEWDDALSKARFEFRWRDQFALSLDPVTAQSFHDETLPAEGAKVAHFCSMCGPKFCSMSITEDVRKYAEEHGYGDADEAIKQGMEMMSAEFMNVKKTISGEQWGETGGEIYVPESYIAGANGK 646          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|168064850|ref|XP_001784371.1| (predicted protein [Physcomitrella patens] >gi|162664107|gb|EDQ50840.1| predicted protein [Physcomitrella patens])

HSP 1 Score: 921.383 bits (2380), Expect = 0.000e+0
Identity = 441/589 (74.87%), Postives = 491/589 (83.36%), Query Frame = 0

Query:   69 RPAGS-----GRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVD-EKVFEVGMENMSKEFAKV-------------GQVYTEETELAKKL 638
            RP+ S     GR++PTKDP  PDF    +FEE +P  TKE   V HE TG V+ VPFRR+HL+  DP   H D YDTSGPQ VNPR GLPKLRK W+ RRE   D   TQM+YAK+GI+TEEMAY AARE MDP+FV SEVARGRAIIPSNK+HLELEPMIVGR F VKVN+NIGNSAVTSSIEEEV+KLQW+T+WGADT+MDLSTG HIH+TREWIMRN+ +PVGTVPIYQALEKVDGI ENLTWEVFRETLIEQAEQGVDYFTIHAGVLLR+IPMTA RMTGIVSRGGSIHAK CL  HKENFAY HWDDILDIC +YDI+LSIGDGLRPGSI DAND AQF+EL TQGELTRRAWEK+VQVMNEGPGH+PLHKIPENM  QLDWCSEAPFYTLGPLTTD+APGYDHITSAIGAA IG+LGTALLCYVTPKEHLGLPNRDDVKAG+IAY+IAAHAADLAKQHP AQ WDDALSK+RFEFRW DQF L++DP+TA ++HDET+ ++  K AHFCSMCGPKFCSM ITEDVR YA +HGYG + E   + GM+ MS+EF                G++Y  E+ +A KL
Sbjct:    7 RPSSSSVHSAGRTRPTKDPAAPDFKPIPSFEECFPSSTKEIKEVVHEPTGSVLQVPFRRIHLSGDDP---HFDTYDTSGPQKVNPRYGLPKLRKEWIDRREALKDERFTQMYYAKKGIITEEMAYCAAREKMDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVTSSIEEEVQKLQWATMWGADTIMDLSTGLHIHETREWIMRNSSVPVGTVPIYQALEKVDGIAENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRFIPMTARRMTGIVSRGGSIHAKWCLAHHKENFAYTHWDDILDICNQYDIALSIGDGLRPGSIYDANDMAQFSELATQGELTRRAWEKDVQVMNEGPGHIPLHKIPENMEKQLDWCSEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPLAQSWDDALSKARFEFRWRDQFALSLDPVTALSFHDETLPAEGAKVAHFCSMCGPKFCSMSITEDVRRYAEEHGYGDEAEAAIKDGMDKMSREFLAAKKTISGEQWGESGGEIYLPESYVASKL 592          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|1040842999|ref|XP_017234844.1| (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 [Daucus carota subsp. sativus])

HSP 1 Score: 917.531 bits (2370), Expect = 0.000e+0
Identity = 424/558 (75.99%), Postives = 481/558 (86.20%), Query Frame = 0

Query:   70 PAGSGRSKPTK------DPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEF 621
            P+ + + K TK      DP  PDF+    F++ +P+ TKEY  VTHE TG V+ VPFRR+HLA  +P   H D YDTSGPQ ++PR GLPKLRK W+ RRE+ G    TQM+YAK+GI+TEEM Y AARE +DP+FV +EVARGRAIIPSNK+HLELEPMIVGRNF VKVN+NIGNSAV SSIEEEV+KLQW+T+WGADT+MDLSTG+HIH+TREWI+RN+ +PVGTVPIYQALEKVDGI ENL WEVFRETLIEQAEQGVDYFTIHAGVLLRYIP+TA RMTGIVSRGGSIHAK CL  HKENFAYEHWDDILDIC +YDI+LSIGDGLRPGSI DAND AQFAELLTQGELTRRAWEK+VQVMNEGPGH+P+HKIPENM+ QL+WC+EAPFYTLGPLTTD+APGYDHITSAIGAA IG+LGTALLCYVTPKEHLGLPNRDDVK G+I+Y+IAAHAADLAK HP AQ WDDALSK+RFEFRW DQF L++DP+TA ++HDET+ SD  K AHFCSMCGPKFCSMKITEDVR YA +HGYG  E+  + GM+ MS EF
Sbjct:  650 PSSTNKEKTTKHRKHTVDPAAPDFLPLPAFDQCFPRSTKEYTEVTHEETGHVLKVPFRRIHLAGDEP---HFDTYDTSGPQDISPRIGLPKLRKEWIDRREKLGLPRFTQMFYAKKGIITEEMVYCAAREKLDPEFVRAEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVVSSIEEEVQKLQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAENLNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLTYHKENFAYEHWDDILDICNQYDIALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQAWDDALSKARFEFRWLDQFALSLDPMTATSFHDETLPSDGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSAEEAVQQGMDAMSAEF 1204          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|659115105|ref|XP_008457389.1| (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis melo])

HSP 1 Score: 916.376 bits (2367), Expect = 0.000e+0
Identity = 429/574 (74.74%), Postives = 486/574 (84.67%), Query Frame = 0

Query:   48 AAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEF 621
            A  T + P+T+ E            +  SK T DP +PDF+   +FE+ +PK TKE+  V HE TG V+ VPFRRVHL+  +P   + D YDTSGPQ +NPR GLPKLRK WV RR++ G    TQM+YAKQGI+TEEM + A RE +DP+FV SEVARGRAIIPSNK HLELEPMIVGRNF VKVN+NIGNSAV SSIEEEV K+QW+T+WGADT+MDLSTG+HIH+TREWI+RN+ +PVGTVPIYQALEKV+GI ENLTWE+FRETLIEQAEQGVDYFTIHAGVLLRYIP+TA RMTGIVSRGGSIHAK CL  HKENFAYEHWDDILDIC +YDISLSIGDGLRPGSI DAND AQFAELLTQGELTRRAWEK+VQVMNEGPGH+P+HKIPENM+ QL+WC+EAPFYTLGPLTTDVAPGYDHITSAIGAA IG+LGTALLCYVTPKEHLGLPNRDDVKAG+IAY+IAAHAADLAK HP AQ WDDALSK+RFEFRW DQF L++DP+TA ++HDET+ S+  K AHFCSMCGPKFCSMKITEDVR YA +HGYG  E+  + GM+ MS EF
Sbjct:  477 ATLTFDPPETSTEK-----------AKNSKHTIDPSSPDFLPLPSFEQCFPKSTKEHKEVVHEETGHVLKVPFRRVHLSGDEP---NFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGAPRCTQMYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGTAEEALQQGMDAMSAEF 1036          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|778661672|ref|XP_011658531.1| (PREDICTED: phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 915.99 bits (2366), Expect = 0.000e+0
Identity = 424/545 (77.80%), Postives = 476/545 (87.34%), Query Frame = 0

Query:   77 KPTKDPYNPDFIRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEF 621
            K T DP +PDF+   +FE+ +PK TKE+  V HE TG V+ VPFRRVHL+  +P   + D YDTSGPQ +NPR GLPKLRK WV RR++ G    TQM+YAKQGI+TEEM Y A RE +DP+FV SEVARGRAIIPSNK HLELEPMIVGRNF VKVN+NIGNSAV SSIEEEV K+QW+T+WGADT+MDLSTG+HIH+TREWI+RN+ +PVGTVPIYQALEKV+GI ENLTWE+FRETLIEQAEQGVDYFTIHAGVLLRYIP+TA RMTGIVSRGGSIHAK CL  HKENFAYEHWDDILDIC +YDISLSIGDGLRPGSI DAND AQFAELLTQGELTRRAWEK+VQVMNEGPGH+P+HKIPENM+ QL+WC+EAPFYTLGPLTTDVAPGYDHITSAIGAA IG+LGTALLCYVTPKEHLGLPNRDDVKAG+IAY+IAAHAADLAK HP AQ WDDALSK+RFEFRW DQF L++DP+TA ++HDET+ S+  K AHFCSMCGPKFCSMKITEDVR YA +HGYG  E+  + GM+ MS EF
Sbjct:  667 KHTIDPSSPDFLPLPSFEQCFPKSTKEHTEVVHEETGHVLKVPFRRVHLSGDEP---NFDNYDTSGPQNINPRIGLPKLRKDWVDRRDKLGSPRYTQMYYAKQGIITEEMLYCATREKLDPEFVRSEVARGRAIIPSNKNHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSSVPVGTVPIYQALEKVNGIAENLTWEIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHIPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSLDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGSAEEALQEGMDAMSAEF 1208          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|168028666|ref|XP_001766848.1| (predicted protein [Physcomitrella patens] >gi|162681827|gb|EDQ68250.1| predicted protein [Physcomitrella patens])

HSP 1 Score: 913.679 bits (2360), Expect = 0.000e+0
Identity = 440/635 (69.29%), Postives = 505/635 (79.53%), Query Frame = 0

Query:   20 GSCVEGWLVGSGVKATGKLTMRKIETSLAAATIEAPKTTAEGIGAEGVIRPAGSGRSKPTKDPYNPDFIRPLTFEEAYPKCTKE--YIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVD-EKVFEVGMENMSKEFAKV-------------GQVYTEETELAKKL 638
            GSCVE     S    + K+TM         A +  P ++A G+G           R++PT DP  PDF     FEE +P  TKE  +I V H  TG V+ VPFRR+HL+  DP   H D YDTSGPQ VNPR+GLPK+RK W+ RRE + D   TQM+YAK+GI+TEEM + AARE MDP++V +EVARGRAIIPSNK+HLELEPMIVGR F VKVN+NIGNSAVTSSIEEEV+KLQW+T+WGADT+MDLSTG HIH+TREWIMRN+ +PVGTVPIYQALEKV+GI E+LTWEVFRETLIEQAEQGVDYFTIHAGVLLR+IPMTA RMTGIVSRGGSIHAK CL  HKENFAY HWDDILDIC +YDI+LSIGDGLRPGSI DAND AQF+EL TQGELTRRAWEK+VQVMNEGPGH+PLHKIPENM  QLDWC+EAPFYTLGPLTTD+APGYDHITSAIGAA IG+LGTALLCYVTPKEHLGLPNRDDVK G+IAY+IAAHAADLAK+HP AQ WDDALSK+RFEFRW DQF L++DP+TA ++HDET+ ++  K AHFCSMCGPKFCSM ITEDVR YA +HGYG + E   + GME MS+EF                G++Y  E+ +A K+
Sbjct:   53 GSCVER----SSRNVSMKVTM--------TAILPRPSSSASGVG-----------RTRPTSDPAAPDFKPIPAFEECFPNSTKETMHIEVVHSTTGSVLQVPFRRIHLSGDDP---HFDTYDTSGPQNVNPRDGLPKIRKEWIARREARKDERFTQMYYAKKGIITEEMVFCAAREKMDPEYVRTEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVTSSIEEEVQKLQWATMWGADTIMDLSTGLHIHETREWIMRNSSVPVGTVPIYQALEKVNGIAEDLTWEVFRETLIEQAEQGVDYFTIHAGVLLRFIPMTARRMTGIVSRGGSIHAKWCLAHHKENFAYTHWDDILDICNQYDIALSIGDGLRPGSIYDANDMAQFSELATQGELTRRAWEKDVQVMNEGPGHIPLHKIPENMEKQLDWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVIAYKIAAHAADLAKRHPLAQNWDDALSKARFEFRWRDQFALSLDPVTALSFHDETLPAEGAKVAHFCSMCGPKFCSMSITEDVRKYAEEHGYGDEAEAAMKDGMEKMSQEFLAAKKTISGEQWGESGGEIYLPESYVASKI 661          

BLAST of EWM27469.1 vs. NCBI_GenBank
Match: gi|397572785|gb|EJK48404.1| (hypothetical protein THAOC_32799 [Thalassiosira oceanica])

HSP 1 Score: 911.753 bits (2355), Expect = 0.000e+0
Identity = 430/563 (76.38%), Postives = 494/563 (87.74%), Query Frame = 0

Query:   74 GRSKPTKDPYNPDF--IRPLTFEEAYPKCTKEYIPVTHEGTGEVMSVPFRRVHLADTDPGCEHLDLYDTSGPQGVNPREGLPKLRKSWVGRREQKGDTTPTQMWYAKQGIVTEEMAYVAAREGMDPQFVLSEVARGRAIIPSNKRHLELEPMIVGRNFKVKVNSNIGNSAVTSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNAPIPVGTVPIYQALEKVDGIVENLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPMTASRMTGIVSRGGSIHAKLCLLDHKENFAYEHWDDILDICAKYDISLSIGDGLRPGSIKDANDEAQFAELLTQGELTRRAWEKNVQVMNEGPGHVPLHKIPENMRNQLDWCSEAPFYTLGPLTTDVAPGYDHITSAIGAATIGSLGTALLCYVTPKEHLGLPNRDDVKAGLIAYRIAAHAADLAKQHPRAQEWDDALSKSRFEFRWEDQFNLAMDPITARAYHDETIDSDAGKNAHFCSMCGPKFCSMKITEDVRAYARDHGYGVDEKVFEVGMENMSKEFAKVG-QVYTEETE 633
            GR+KPT DP+NP+F  I+ + + +A+P  TKEY   TH  TG  + VPFRRVHL D  P    LDLYDTSGP+ V+P++GLPK+RK+WV  RE K +   TQM +A++G++TEEM YVA REGM  +FV SEVARGRAII SNK HLELEP I+GR FKVK+N+NIGNSA+TSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRN+PIPVGTVPIYQALEKVDGI E+LTWEVFRETLIEQAEQGVDYFTIHAGVLLRY+PMT SR TGIVSRGGSIHAK  +  HKENFAYEHWDDIL+ICA+YDI+LSIGDGLRPGSI DANDE+QFAEL+TQG+LTRRAWEK+VQVMNEGPGHVPLHKIPENMRNQLDWC+EAPFYTLGPLTTD+APGYDHITSAIGAA I SLGTA+LCYVTPKEHLGLPNRDDVKAG+IAY+IAAHAADLAK+ P AQ+ DD+LSK+RFEFRW DQFNL++DP+TARA+HDET+DSDA K +HFCSMCGPKFCSMKITEDVR YA ++GYGVDE+  + GM+ MS+ + + G ++Y ++ E
Sbjct:  172 GRTKPTIDPFNPEFELIQGVPYNDAFPNSTKEYKTATHAETGHELKVPFRRVHLED--PDQPSLDLYDTSGPRDVDPKQGLPKVRKTWVDEREGKFERY-TQMHFARRGMITEEMQYVAEREGMSAEFVRSEVARGRAIICSNKNHLELEPQIIGRMFKVKINANIGNSAITSSIEEEVEKLQWSTLWGADTLMDLSTGKHIHQTREWIMRNSPIPVGTVPIYQALEKVDGIAEDLTWEVFRETLIEQAEQGVDYFTIHAGVLLRYVPMTVSRKTGIVSRGGSIHAKWNIFHHKENFAYEHWDDILEICAEYDIALSIGDGLRPGSIYDANDESQFAELMTQGDLTRRAWEKDVQVMNEGPGHVPLHKIPENMRNQLDWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIASLGTAMLCYVTPKEHLGLPNRDDVKAGIIAYKIAAHAADLAKELPGAQDRDDSLSKARFEFRWNDQFNLSLDPVTARAFHDETLDSDASKTSHFCSMCGPKFCSMKITEDVRQYAEENGYGVDEEAIQKGMDLMSEVYKENGNKLYLDDIE 731