EWM27580.1, cds3127 (CDS) Nannochloropsis gaditana

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|585109847|gb|EWM27580.1| (anaphase-promoting complex subunit 4 [Nannochloropsis gaditana])

HSP 1 Score: 1901.72 bits (4925), Expect = 0.000e+0
Identity = 940/940 (100.00%), Postives = 940/940 (100.00%), Query Frame = 0

Query:    1 MQSQHEGMKSARMQPPTFHSQAFSVLHEKSIAVSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAASKRLTFRHSTKHTRMLLSFLRKPVAASAPTDSEDAPGEKRAAGPTGNTEAVIAVHISSYFKDQPLPMPPSRIMTTSAPARSPGSCDAVDGGAKEGAKGLASVSLRTQVKWVLARFEAVFRRPMEAITDRLSSPYVLLLSQGPLASPPSLYAPARSDPSRILMAAVSSGGARTIVLSIEVGALASRMRLGAETPNGIPHVEVTGLSLDAPAGFSFQQAALYGSRPHIPSSNPESIVLLVRENVQPFEIRTPAELWKLPLTSLLTHLNPVDKEGGARACLLDGQGARSLLPWEMVSNVRTRQVAAVAPRKLALAVTGCRGIACVTSESNFLLLADLEEDEDEDEEEEGNEGEGQGVGRVTRLQEQLDGLTNEVMQVESTMRMEIVQGEENEDAKSAC 940
            MQSQHEGMKSARMQPPTFHSQAFSVLHEKSIAVSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAASKRLTFRHSTKHTRMLLSFLRKPVAASAPTDSEDAPGEKRAAGPTGNTEAVIAVHISSYFKDQPLPMPPSRIMTTSAPARSPGSCDAVDGGAKEGAKGLASVSLRTQVKWVLARFEAVFRRPMEAITDRLSSPYVLLLSQGPLASPPSLYAPARSDPSRILMAAVSSGGARTIVLSIEVGALASRMRLGAETPNGIPHVEVTGLSLDAPAGFSFQQAALYGSRPHIPSSNPESIVLLVRENVQPFEIRTPAELWKLPLTSLLTHLNPVDKEGGARACLLDGQGARSLLPWEMVSNVRTRQVAAVAPRKLALAVTGCRGIACVTSESNFLLLADLEEDEDEDEEEEGNEGEGQGVGRVTRLQEQLDGLTNEVMQVESTMRMEIVQGEENEDAKSAC
Sbjct:    1 MQSQHEGMKSARMQPPTFHSQAFSVLHEKSIAVSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAASKRLTFRHSTKHTRMLLSFLRKPVAASAPTDSEDAPGEKRAAGPTGNTEAVIAVHISSYFKDQPLPMPPSRIMTTSAPARSPGSCDAVDGGAKEGAKGLASVSLRTQVKWVLARFEAVFRRPMEAITDRLSSPYVLLLSQGPLASPPSLYAPARSDPSRILMAAVSSGGARTIVLSIEVGALASRMRLGAETPNGIPHVEVTGLSLDAPAGFSFQQAALYGSRPHIPSSNPESIVLLVRENVQPFEIRTPAELWKLPLTSLLTHLNPVDKEGGARACLLDGQGARSLLPWEMVSNVRTRQVAAVAPRKLALAVTGCRGIACVTSESNFLLLADLEEDEDEDEEEEGNEGEGQGVGRVTRLQEQLDGLTNEVMQVESTMRMEIVQGEENEDAKSAC 940          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|553196075|ref|XP_005856263.1| (anaphase promoting complex subunit 4, partial [Nannochloropsis gaditana CCMP526] >gi|422292796|gb|EKU20098.1| anaphase promoting complex subunit 4, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 291.967 bits (746), Expect = 3.774e-90
Identity = 140/142 (98.59%), Postives = 141/142 (99.30%), Query Frame = 0

Query:   40 PKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFA 181
            PKMDL+ALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPAS VQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFA
Sbjct:    1 PKMDLIALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASAVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFA 142          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|298711419|emb|CBJ32560.1| (Putative subunit of the Promoting Anaphase Complex [Ectocarpus siliculosus])

HSP 1 Score: 154.066 bits (388), Expect = 1.173e-34
Identity = 178/677 (26.29%), Postives = 278/677 (41.06%), Query Frame = 0

Query:   23 FSVLHEKSIAVSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQ-REPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPLGLQSGSFAVPRHVKQGSIEAKLAAGVASL--------DVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPS-ILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLL--RDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSA------LSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPT--------GTEEPPAASKRLTFRHSTKHTRMLLSFLRKPVAASAPTDSEDAPGEKRAAG--PTGNTEAVIAVHISSYFKD----QPLPMPPSRIMTTSAPARSPGSCDAVDGGAKEGAKGLASVSLRTQVKWVLARFEAVFRRP 667
            FS++HEK +    V +CP MDL+A L  DG + V RT+ WQR  TL    + + +  + ++WSP+G  LA+G+  G I    VE G           A    + + AL W  QQ R     S  D E +        Y DR          L   +G+       +     ++   G   L         VL +    G +++   G   ++++ LR    PT     A   GR   V SP   RQ R    P    A  +    S + +D            P L P  L++     S DLS +   +       G V  + V   +LW  RH+L+PL ++  A   ++  L+ ++    + W  +++ L+ KL  L  LL    Y  + +A++  +  V  G A  AL+Q+LS   +  Q   + R+ K +D     VE +LR  V+ +   + FRA  L  L  +      +            + A +K    L VK E+TLR+VQ +R  ++  +LWL    + + P         GTEE    S  LT  +S      +L+ LR+P+    P+D         AAG  P    E++I   +     D    + L  PP+       P   P   +      ++    LAS +LR QV+ + A  E VF+ P
Sbjct:    8 FSIIHEKLLHCRLVCMCPTMDLVASLHVDGQLSVHRTMGWQRSFTLTQEDMGN-RTFSCVTWSPNGKTLAVGYTDGTIQLFGVETGAEE-----CSLAFHEGRSLAALTWVAQQPRHGTARSRADEEADQLAEVAAAYADRCEHVLGPCDQLPGANGAPGGGVAGRSSEYSSQADDGFMLLQGMKERPVSVLASADSAGVVVLSICGAYRLITIDLRAHRKPTKGKGRASGGGR---VDSP---RQRRFRRSPGQDGAGFSGLTESEVDID---------GTGPALRPLQLTL-----SADLSVLTALVEFD----GQVELIQVDLPLLWRQRHELKPLAMQFTAFRAVIGRLVVSVPRLPQLWKGTLQALETKLGNLEDLLPRFGYDKDITAKDEFIHLVTCGYASAALAQFLSTGLTHVQ---LARMRKAVDTGTGQVEAMLRGDVSLLARTLLFRACDLHRLVIACTGGGGVDGDGGLGLGQAPVEAFLKDCEMLCVKVEQTLRDVQTSRSRLRFLLLWL----EQMSPVVTDSNSADGTEEHEPPSPCLTLANS------VLTILREPL----PSDKPSLSSRSTAAGASPRSRVESMIGTRVPGLAVDENPVESLEGPPA----ARGPLTGPNQKNRFVVLPEDPV--LASRTLRGQVQRLGASVEEVFQAP 631          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1002238088|ref|XP_015623248.1| (PREDICTED: anaphase-promoting complex subunit 4 isoform X1 [Oryza sativa Japonica Group])

HSP 1 Score: 119.783 bits (299), Expect = 5.816e-24
Identity = 124/523 (23.71%), Postives = 218/523 (41.68%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAAS----PTLSPPAL-SVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAAS 546
            +   E  P+ DLLA++ TD S VVL   +WQRL T+        K +T++ WSPDG ++ALG   G +   DVE G+  R+      A      +  L W     E   +S  D +  L+      Y DR A F+   P +    GL SG   +     + SI+   +A     ++L +  +GG I     G+ PV                                    + N+   P   + + +  S  L   S S +  + +      L P  L  + +L+ S  +S              G+  L + T++ +  +++L  ++ + +++  LV  +  +L M  +QW+ ++   + K   L  L+  +  E+S+ +  +S +      PAL Q+L  +  E   +   R+ K +D     +  ++ E +    E+I+FR  +LRGLAR    +Q +GL    +  + +  G L V+ E   R         + F  W+L  ++ L    T++ PAA+
Sbjct:   31 IKMAEWNPEKDLLAMV-TDDSKVVLHRFNWQRLWTISPG-----KCITSICWSPDGKIVALGTEDGLVLLHDVENGKMLRRIKSHDVA------IVCLNW----AEDELLSRTDKDGLLS------YEDRTARFFPPAPVIPRIGGLSSGDTGLSDE-NEESIQEFSSASCQRFNILCSGDKGGCICFSIFGIFPV-----------------------------------GKININEVPIHFQSSGNKTSYRLQDASISKVCLSGNLHQLVLLCPGKLIDIDNLSHSNHIST-------------GLHCLHLDTSIFFNRKNELHQISQQASSIQDLVEVVRSSLSMMAKQWSSAMNLFNEKFSALPSLIAAHGMESSSEDEFMSLLFGTRTSPALHQFLVSSLGEAALK---RIAKAVDSAGRELRVVVSEHLQPAVEIISFRLAELRGLARWRSRFQNVGLDEKLIYGVTEKIGMLVVQVERFSRVAATVLYLFQNFFSWVLKCVKILLSEPTDQVPAAN 479          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1002238090|ref|XP_015623249.1| (PREDICTED: anaphase-promoting complex subunit 4 isoform X2 [Oryza sativa Japonica Group])

HSP 1 Score: 119.398 bits (298), Expect = 6.328e-24
Identity = 124/523 (23.71%), Postives = 218/523 (41.68%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAAS----PTLSPPAL-SVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAAS 546
            +   E  P+ DLLA++ TD S VVL   +WQRL T+        K +T++ WSPDG ++ALG   G +   DVE G+  R+      A      +  L W     E   +S  D +  L+      Y DR A F+   P +    GL SG   +     + SI+   +A     ++L +  +GG I     G+ PV                                    + N+   P   + + +  S  L   S S +  + +      L P  L  + +L+ S  +S              G+  L + T++ +  +++L  ++ + +++  LV  +  +L M  +QW+ ++   + K   L  L+  +  E+S+ +  +S +      PAL Q+L  +  E   +   R+ K +D     +  ++ E +    E+I+FR  +LRGLAR    +Q +GL    +  + +  G L V+ E   R         + F  W+L  ++ L    T++ PAA+
Sbjct:   31 IKMAEWNPEKDLLAMV-TDDSKVVLHRFNWQRLWTISPG-----KCITSICWSPDGKIVALGTEDGLVLLHDVENGKMLRRIKSHDVA------IVCLNW----AEDELLSRTDKDGLLS------YEDRTARFFPPAPVIPRIGGLSSGDTGLSDE-NEESIQEFSSASCQRFNILCSGDKGGCICFSIFGIFPV-----------------------------------GKININEVPIHFQSSGNKTSYRLQDASISKVCLSGNLHQLVLLCPGKLIDIDNLSHSNHIST-------------GLHCLHLDTSIFFNRKNELHQISQQASSIQDLVEVVRSSLSMMAKQWSSAMNLFNEKFSALPSLIAAHGMESSSEDEFMSLLFGTRTSPALHQFLVSSLGEAALK---RIAKAVDSAGRELRVVVSEHLQPAVEIISFRLAELRGLARWRSRFQNVGLDEKLIYGVTEKIGMLVVQVERFSRVAATVLYLFQNFFSWVLKCVKILLSEPTDQVPAAN 479          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1142692091|gb|AQK56017.1| (Anaphase-promoting complex subunit 4 [Zea mays])

HSP 1 Score: 118.627 bits (296), Expect = 7.958e-24
Identity = 120/523 (22.94%), Postives = 221/523 (42.26%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTS-PASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAASKRLT 550
            +   E  P+ DLLA++ TD S V+L   +WQRL T+        K +T++ WSPDG ++ALG   G I   DVE G+  R       A      + +L W     E   +S  D ++ L+      Y DR   F+   P +    GL+SG   +    ++   E   +A     ++L +  + G +     G+ PV                     G+ I +T  P ++  +R + Q   +     S      L    Q F+            L    L  + +LS              G+  L + T++ +  +++L  ++ + +++  +V  +  ++ +  +QW+ ++     K   L  L+  +  E+S+ +  LS +      PAL  +L+ +  E     + R+ K +D     + G++ E +    E+I+FR  +LRGL+R    +Q +GL  + +  + ++ G L V+ E   R         + F  W+L +++ L    T++ PAA+  L 
Sbjct:   31 IKMAEWNPEKDLLAMV-TDDSKVLLHRFNWQRLWTISPG-----KCITSICWSPDGKIIALGTEDGLILLHDVENGKMLRTTKSHDVA------IVSLNW----AEDDPLSKSDKDEFLS------YEDRTTRFFPPPPVMPRIGGLRSGDTGLADENEEAIPEFS-SASCQRFNILCSGGKDGCVCFSVFGIFPV---------------------GK-INITKIPINVGSSRKSYQLQDASVSKVS------LSRNLQKFV-----------ILCFGKLVDTDNLS--------DSCENSGLHCLYLDTSIFFNRKNELHQVSQQASSIQDMVEVVRASVSLISKQWSNAMSLFHEKFSALPNLISTHGVESSSEDEFLSLLFGTRTSPALHHFLASSLGEA---GLKRIAKAVDSAGRDIRGIITEHLQPAVEIISFRLAELRGLSRWRSRFQTIGLDGNLIDGVTESIGMLVVQVERFSRVAATVVYLFQNFFAWVLKSVRILLNEPTDQVPAANSELV 480          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1002842731|ref|XP_015688990.1| (PREDICTED: anaphase-promoting complex subunit 4 isoform X1 [Oryza brachyantha])

HSP 1 Score: 118.627 bits (296), Expect = 1.257e-23
Identity = 122/519 (23.51%), Postives = 217/519 (41.81%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPAL-SVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAAS 546
            +   E  P+ DLLA++ TD S V+L   +WQRL T+        K +T++ WSPDG ++ALG   G +   DVE G+  R+        +    +  L W   + EP + + +D            Y DR   F+   P +    GL SG   +     + SI+   +A     ++L +  +GG I     G+ PV  + + D       P     +G                    T    + AS     L  +  Q  L       L P  L  + +L+ S  +S              G+  L + T++ +  +++L  ++ + +++  LV  +  ++ M  +QW+ ++   + K   L  L+  +  E+S+ +  +S +      PAL Q+L  +  E   +   R+ K +D     +  ++ E +    E+I+FR  +LRGLAR    +Q +GL    +  + +  G L V+ E   R         + F  W+L  ++ L    T++ PAA+
Sbjct:   31 IKMAEWNPEKDLLAMV-TDDSKVILHRFNWQRLWTISPG-----KCITSICWSPDGKIVALGTEDGLVLLHDVENGKMLRR------IKSHDVAIVCLNW--AEDEPLSRTDKD--------GLLSYEDRTTRFFPPAPVIPRIGGLNSGDTGLADE-NEESIQEFSSASCHRFNILCSGDKGGCICFSIFGIFPVGKININD------VPIHVQSSG------------------NKTCYQLQDASISKVCLSGNLHQLVL-------LCPGKLVDIDNLSLSNHISV-------------GLHCLHLDTSIFFNRKNELHQISQQASSIQDLVEVVRASISMMAKQWSSAMNLFNEKFSALPSLIAAHGMESSSEDEFMSLLFGTRTSPALHQFLVSSLGEAALK---RIAKAVDSAGRELRVVVSEHLQPAVEIISFRLAELRGLARWRSRFQNIGLDEKLIDGVTEKIGMLVVQVERFSRVAATVLYLFQNFFSWVLKCVKILLSEPTDQVPAAN 479          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1142692089|gb|AQK56015.1| (Anaphase-promoting complex subunit 4 [Zea mays])

HSP 1 Score: 118.627 bits (296), Expect = 1.416e-23
Identity = 120/523 (22.94%), Postives = 221/523 (42.26%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTS-PASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAASKRLT 550
            +   E  P+ DLLA++ TD S V+L   +WQRL T+        K +T++ WSPDG ++ALG   G I   DVE G+  R       A      + +L W     E   +S  D ++ L+      Y DR   F+   P +    GL+SG   +    ++   E   +A     ++L +  + G +     G+ PV                     G+ I +T  P ++  +R + Q   +     S      L    Q F+            L    L  + +LS              G+  L + T++ +  +++L  ++ + +++  +V  +  ++ +  +QW+ ++     K   L  L+  +  E+S+ +  LS +      PAL  +L+ +  E     + R+ K +D     + G++ E +    E+I+FR  +LRGL+R    +Q +GL  + +  + ++ G L V+ E   R         + F  W+L +++ L    T++ PAA+  L 
Sbjct:   31 IKMAEWNPEKDLLAMV-TDDSKVLLHRFNWQRLWTISPG-----KCITSICWSPDGKIIALGTEDGLILLHDVENGKMLRTTKSHDVA------IVSLNW----AEDDPLSKSDKDEFLS------YEDRTTRFFPPPPVMPRIGGLRSGDTGLADENEEAIPEFS-SASCQRFNILCSGGKDGCVCFSVFGIFPV---------------------GK-INITKIPINVGSSRKSYQLQDASVSKVS------LSRNLQKFV-----------ILCFGKLVDTDNLS--------DSCENSGLHCLYLDTSIFFNRKNELHQVSQQASSIQDMVEVVRASVSLISKQWSNAMSLFHEKFSALPNLISTHGVESSSEDEFLSLLFGTRTSPALHHFLASSLGEA---GLKRIAKAVDSAGRDIRGIITEHLQPAVEIISFRLAELRGLSRWRSRFQTIGLDGNLIDGVTESIGMLVVQVERFSRVAATVVYLFQNFFAWVLKSVRILLNEPTDQVPAANSELV 480          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1142692097|gb|AQK56023.1| (Anaphase-promoting complex subunit 4 [Zea mays])

HSP 1 Score: 118.242 bits (295), Expect = 1.514e-23
Identity = 120/523 (22.94%), Postives = 221/523 (42.26%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTS-PASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPALSVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAASKRLT 550
            +   E  P+ DLLA++ TD S V+L   +WQRL T+        K +T++ WSPDG ++ALG   G I   DVE G+  R       A      + +L W     E   +S  D ++ L+      Y DR   F+   P +    GL+SG   +    ++   E   +A     ++L +  + G +     G+ PV                     G+ I +T  P ++  +R + Q   +     S      L    Q F+            L    L  + +LS              G+  L + T++ +  +++L  ++ + +++  +V  +  ++ +  +QW+ ++     K   L  L+  +  E+S+ +  LS +      PAL  +L+ +  E     + R+ K +D     + G++ E +    E+I+FR  +LRGL+R    +Q +GL  + +  + ++ G L V+ E   R         + F  W+L +++ L    T++ PAA+  L 
Sbjct:   31 IKMAEWNPEKDLLAMV-TDDSKVLLHRFNWQRLWTISPG-----KCITSICWSPDGKIIALGTEDGLILLHDVENGKMLRTTKSHDVA------IVSLNW----AEDDPLSKSDKDEFLS------YEDRTTRFFPPPPVMPRIGGLRSGDTGLADENEEAIPEFS-SASCQRFNILCSGGKDGCVCFSVFGIFPV---------------------GK-INITKIPINVGSSRKSYQLQDASVSKVS------LSRNLQKFV-----------ILCFGKLVDTDNLS--------DSCENSGLHCLYLDTSIFFNRKNELHQVSQQASSIQDMVEVVRASVSLISKQWSNAMSLFHEKFSALPNLISTHGVESSSEDEFLSLLFGTRTSPALHHFLASSLGEA---GLKRIAKAVDSAGRDIRGIITEHLQPAVEIISFRLAELRGLSRWRSRFQTIGLDGNLIDGVTESIGMLVVQVERFSRVAATVVYLFQNFFAWVLKSVRILLNEPTDQVPAANSELV 480          

BLAST of EWM27580.1 vs. NCBI_GenBank
Match: gi|1002842733|ref|XP_015688991.1| (PREDICTED: anaphase-promoting complex subunit 4 isoform X2 [Oryza brachyantha])

HSP 1 Score: 118.242 bits (295), Expect = 1.674e-23
Identity = 122/519 (23.51%), Postives = 217/519 (41.81%), Query Frame = 0

Query:   33 VSKVELCPKMDLLALLRTDGSVVVLRTISWQRLNTLPAATLQDEKRVTAMSWSPDGNVLALGHAQGAISFLDVEKGERARQPLGARPASTVQQGVEALQWFTQQREPATMSSEDWEKNLTQAAGKYYLDRDAVFWEELPPL----GLQSGSFAVPRHVKQGSIEAKLAAGVASLDVLVACFQGGHILVFSSGLLPVVSLRLRDFFPPTPAPSLADENGRHIGVTSPASIRQARTNVQPTPSPAKPASSPPSILLDHPSQSFLPRAASPTLSPPAL-SVQSLAASRDLSAMVFTIAAGEGSRGGVSTLVVPTAVLWTHRHQLRPLTVELAAVSGLVSHLLEALDMAERQWNESVRTLDIKLELLNGLLRDYACEASAREALLSFVLSGVACPALSQYLSQNGSEPQQQNMFRLLKTMDDKIAGVEGLLREQVARVGEMIAFRAVKLRGLARSGPDYQALGLSTSALSALIKAAGDLSVKAEETLREVQRARQNIKVFVLWLLDTMQHLPPTGTEEPPAAS 546
            +   E  P+ DLLA++ TD S V+L   +WQRL T+        K +T++ WSPDG ++ALG   G +   DVE G+  R+        +    +  L W   + EP + + +D            Y DR   F+   P +    GL SG   +     + SI+   +A     ++L +  +GG I     G+ PV  + + D       P     +G                    T    + AS     L  +  Q  L       L P  L  + +L+ S  +S              G+  L + T++ +  +++L  ++ + +++  LV  +  ++ M  +QW+ ++   + K   L  L+  +  E+S+ +  +S +      PAL Q+L  +  E   +   R+ K +D     +  ++ E +    E+I+FR  +LRGLAR    +Q +GL    +  + +  G L V+ E   R         + F  W+L  ++ L    T++ PAA+
Sbjct:   25 IKMAEWNPEKDLLAMV-TDDSKVILHRFNWQRLWTISPG-----KCITSICWSPDGKIVALGTEDGLVLLHDVENGKMLRR------IKSHDVAIVCLNW--AEDEPLSRTDKD--------GLLSYEDRTTRFFPPAPVIPRIGGLNSGDTGLADE-NEESIQEFSSASCHRFNILCSGDKGGCICFSIFGIFPVGKININD------VPIHVQSSG------------------NKTCYQLQDASISKVCLSGNLHQLVL-------LCPGKLVDIDNLSLSNHISV-------------GLHCLHLDTSIFFNRKNELHQISQQASSIQDLVEVVRASISMMAKQWSSAMNLFNEKFSALPSLIAAHGMESSSEDEFMSLLFGTRTSPALHQFLVSSLGEAALK---RIAKAVDSAGRELRVVVSEHLQPAVEIISFRLAELRGLARWRSRFQNIGLDEKLIDGVTEKIGMLVVQVERFSRVAATVLYLFQNFFSWVLKCVKILLSEPTDQVPAAN 473