EWM27685.1, cds2995 (CDS) Nannochloropsis gaditana

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|585109957|gb|EWM27685.1| (two-pore calcium channel [Nannochloropsis gaditana])

HSP 1 Score: 1340.48 bits (3468), Expect = 0.000e+0
Identity = 643/643 (100.00%), Postives = 643/643 (100.00%), Query Frame = 0

Query:    1 MTDKDLPNKQTSEDHDSAGVNSSFQKGADRHEKLTIISQNSVEDDEVRRESQLKKNDKSVLSLRSNDSPPIAKHEIVTPSWGPVSVERDGEVFKAVLFLMDAKKKKQPNFLDNYKSMRWYHARTKFMWKMGLFVACVIYLLVSATDTVSFNSSEDPMHWFWYQVSMQLLCDCVFIGDICAQIQAKGYRTTLNNRWTQFFCLTLVFHSVGIFWPGLQALKVTGIVRAYPIIYYNQHARNAMISYMKTLPSVTLWIGLEFFVIIIYACFCVVLYAHLDRYGESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLRRLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCYSPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            MTDKDLPNKQTSEDHDSAGVNSSFQKGADRHEKLTIISQNSVEDDEVRRESQLKKNDKSVLSLRSNDSPPIAKHEIVTPSWGPVSVERDGEVFKAVLFLMDAKKKKQPNFLDNYKSMRWYHARTKFMWKMGLFVACVIYLLVSATDTVSFNSSEDPMHWFWYQVSMQLLCDCVFIGDICAQIQAKGYRTTLNNRWTQFFCLTLVFHSVGIFWPGLQALKVTGIVRAYPIIYYNQHARNAMISYMKTLPSVTLWIGLEFFVIIIYACFCVVLYAHLDRYGESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLRRLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCYSPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT
Sbjct:    1 MTDKDLPNKQTSEDHDSAGVNSSFQKGADRHEKLTIISQNSVEDDEVRRESQLKKNDKSVLSLRSNDSPPIAKHEIVTPSWGPVSVERDGEVFKAVLFLMDAKKKKQPNFLDNYKSMRWYHARTKFMWKMGLFVACVIYLLVSATDTVSFNSSEDPMHWFWYQVSMQLLCDCVFIGDICAQIQAKGYRTTLNNRWTQFFCLTLVFHSVGIFWPGLQALKVTGIVRAYPIIYYNQHARNAMISYMKTLPSVTLWIGLEFFVIIIYACFCVVLYAHLDRYGESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLRRLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCYSPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|553191663|ref|XP_005855427.1| (two-pore calcium channel [Nannochloropsis gaditana CCMP526] >gi|422293634|gb|EKU20934.1| two-pore calcium channel [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 441.81 bits (1135), Expect = 3.158e-139
Identity = 242/587 (41.23%), Postives = 360/587 (61.33%), Query Frame = 0

Query:   79 PSWGPV-SVERDGEVFKAVLFLMDAKKKKQPNFLDNYKSMRWYHARTKFMWKMGLFVACVIYLLVSATDTVSFN--------SSEDPMHWFWYQVSMQLLCDCVFIGDICAQIQAKGYRTTLNNRWTQFFCLTLVFHSVGIFWPGLQALKVTGIVRAYPIIYYNQHARNAMISYMKTLPSVTLWIGLEFFVIIIYACFCVVLYAHLDRYGESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEAL---DETKDGRLPRALVIHVLRRLWPRYDRAKIEAIMRHTHQYAPREYT--------FDQFEMLMTAL-NDRVSKHRSVRCYSPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLL-PAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            P+ GP+ S  R+ E F+A+L+L+D  +K+Q +F D+ KSMR+Y+ R    ++  + VAC++YL +S T+  +F          S D  H   Y+++  ++    F+ DI  Q QA G + T   RWTQ F + L F  VG+ WP  + L VTGIVR YPI+YY++ ARNAM+ Y++T+PSV  WIG+EF  ++++ACF V+LYA +DR G S Q AF +   AF+SLY LSLTVN+PD+YLPYY+   L+ I+F +FL++T+FL+HNIIL  +++IY  KL++ AV+R   R  AL LAF AL   ++   GR+ + L+ HVL+R++P +   KI A M    QYA RE +        +D+F +LM+A+ +D +   R    Y       +   V A LLV VV++  +W  FY +     ++ FR IF  D+IY + ++ G K ++K   +   + A+L  LVA+V  C+ L  D  +      +  +K A + +L +   D+ R+   +PEY AL+ VF +LL PA L QA VI  LMH+ CY GM ++GG++ P+ P+W   PFPG+LYY LNFN+Y EGM T
Sbjct:  114 PTQGPLLSPRRNLEFFRAILYLLDGGRKQQSHFQDDAKSMRFYYLRQTLAYRTCIIVACIMYLSISLTEAPNFQIYSRKREQVSRDTSHG--YELTFNVISVLFFVLDIWIQFQAMGKKKTFGYRWTQLFVVLLAFDVVGMAWPQARLLMVTGIVRCYPIVYYSKKARNAMLDYLRTVPSVIQWIGIEFLSVLVFACFAVILYASVDRGGASEQTAFLSLNRAFISLYELSLTVNDPDIYLPYYRIDALNAILFVAFLILTFFLLHNIILASVFRIYSAKLRDTAVRRNSYRESALNLAFRALVGENQGPTGRVHKDLLAHVLKRVFPHHSSEKISAFM----QYADREDSNDDDQDYDYDRFLILMSAIADDCLHVIRPSVAYPGRLHSFLDVLVLAELLVAVVAVARYWHIFYKIWLVATLAAFRFIFALDIIYGLLQIKGFKIYLKSSVHKCKLFALLFFLVALV--CM-LTNDRVLGESKEVNMTQKAASMFLLLSVFADVVRLSTRVPEYAALVQVFRDLLVPALLSQATVIFFLMHLTCYAGMYLFGGRIYPSQPIWATLPFPGSLYYLLNFNTYREGMLT 691          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|585109052|gb|EWM26920.1| (two-pore calcium channel [Nannochloropsis gaditana])

HSP 1 Score: 441.425 bits (1134), Expect = 7.085e-139
Identity = 242/587 (41.23%), Postives = 360/587 (61.33%), Query Frame = 0

Query:   79 PSWGPV-SVERDGEVFKAVLFLMDAKKKKQPNFLDNYKSMRWYHARTKFMWKMGLFVACVIYLLVSATDTVSFN--------SSEDPMHWFWYQVSMQLLCDCVFIGDICAQIQAKGYRTTLNNRWTQFFCLTLVFHSVGIFWPGLQALKVTGIVRAYPIIYYNQHARNAMISYMKTLPSVTLWIGLEFFVIIIYACFCVVLYAHLDRYGESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEAL---DETKDGRLPRALVIHVLRRLWPRYDRAKIEAIMRHTHQYAPREYT--------FDQFEMLMTAL-NDRVSKHRSVRCYSPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLL-PAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            P+ GP+ S  R+ E F+A+L+L+D  +K+Q +F D+ KSMR+Y+ R    ++  + VAC++YL +S T+  +F          S D  H   Y+++  ++    F+ DI  Q QA G + T   RWTQ F + L F  VG+ WP  + L VTGIVR YPI+YY++ ARNAM+ Y++T+PSV  WIG+EF  ++++ACF V+LYA +DR G S Q AF +   AF+SLY LSLTVN+PD+YLPYY+   L+ I+F +FL++T+FL+HNIIL  +++IY  KL++ AV+R   R  AL LAF AL   ++   GR+ + L+ HVL+R++P +   KI A M    QYA RE +        +D+F +LM+A+ +D +   R    Y       +   V A LLV VV++  +W  FY +     ++ FR IF  D+IY + ++ G K ++K   +   + A+L  LVA+V  C+ L  D  +      +  +K A + +L +   D+ R+   +PEY AL+ VF +LL PA L QA VI  LMH+ CY GM ++GG++ P+ P+W   PFPG+LYY LNFN+Y EGM T
Sbjct:  114 PTQGPLLSPRRNLEFFRAILYLLDGGRKQQSHFQDDAKSMRFYYLRQTLAYRTCIIVACIMYLSISLTEAPNFQIYSRKREQVSRDTSHG--YELTFNVISVLFFVLDIWIQFQAMGKKKTFGYRWTQLFVVLLAFDVVGMAWPQARLLMVTGIVRCYPIVYYSKKARNAMLDYLRTVPSVIQWIGIEFLSVLVFACFAVILYASVDRGGASEQTAFLSLNRAFISLYELSLTVNDPDIYLPYYRIDALNAILFVAFLILTFFLLHNIILASVFRIYSAKLRDTAVRRNSYRESALNLAFRALVGENQGPTGRVHKDLLAHVLKRVFPHHSSEKISAFM----QYADREDSNDDDQDYDYDRFLILMSAIADDCLHVIRPSVAYPGRLHSFLDVLVLAELLVAVVAVARYWHIFYKIWLVATLAAFRFIFALDIIYGLLQIKGFKIYLKSSVHKCKLFALLFFLVALV--CM-LTNDRVLGESKEVNMTQKAASMFLLLSVFADVVRLSTRVPEYAALVQVFRDLLVPALLSQATVIFFLMHLTCYAGMYLFGGRIYPSQPIWATLPFPGSLYYLLNFNTYREGMLT 691          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|1201315338|gb|OVA14926.1| (EF-hand domain [Macleaya cordata])

HSP 1 Score: 107.842 bits (268), Expect = 1.190e-20
Identity = 89/392 (22.70%), Postives = 157/392 (40.05%), Query Frame = 0

Query:  280 ESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLRRL-----WPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCY--------------------SPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLIS-IFRAIFTADVIYRIKRMGGIKYFI--KDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            + G+  F ++      ++ L  T NNPDV++P YK    + + F  ++++  + + N+IL  +Y  +  +L     +R   R+R LC AF  +D+ K+G L +   I +   L      P+    + E I          +   D+F  L  A+  R  K     C+                    SP    ++AF +  +L+ VV+            + W  +  IF  I+  ++  +I   G   Y+   ++RF+ +    I++   A  A+    +   G    Y            +L A    + R+   +  Y A +  F  L+P  +     I  ++ ++C +G+ I+GG V   NP   E     N Y   NFN Y  GM T
Sbjct:  259 KQGKTTFYSYGVTLYQMFVLFTTSNNPDVWIPAYKESRWNSLFFVIYVLLGVYFLTNLILAVVYDSFKAQLAKQVAERDLMRKRILCKAFNLIDKYKNGFLHKDQCIRLFEELNKYRSLPKISGEEFELIFHELDDSGDFKINLDEFADLCNAIGLRFKKEAIPSCFENYPSFYRSYFSERLKDFVRSPTFGYIIAFILMLNLVAVVIETTLDIENNSGQKVWQEVEFIFGWIYVLEMALKIYSYGFENYWTEGQNRFDFVVTWIIVIGETATFAFPDVPFLSNGEWIRY------------LLIARMLRLIRLLMHVQRYRAFVATFLTLIPTLMPYLGTIFCILCIYCSLGLQIFGGIVNSGNPKLEESALYENDYLLFNFNDYPNGMVT 638          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|747078409|ref|XP_011086365.1| (two pore calcium channel protein 1A [Sesamum indicum])

HSP 1 Score: 102.064 bits (253), Expect = 7.239e-19
Identity = 87/395 (22.03%), Postives = 157/395 (39.75%), Query Frame = 0

Query:  280 ESGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLR-----RLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCYSPCAKRLMAFAVSAHLLVVVVSIIEFWRFFYSVRFWVLIS--------------------------IFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            E G+  F ++ A    ++ L  T NNPDV++P YK    + + F  ++++  + + N+IL  +Y  +  +L     ++ + R R L  AF  +D    G L +   I +       R  P+  R   E I          +   D+F  L  A+  R  K  S   +  C        VS  L   V S     +F Y V F ++++                          +F  ++  ++  ++   G + Y+ +D  N  + +   ++ V ++          G++ L     ++ L     L A    + R+   + +Y A +  F  L+P+ +     I  +M ++C +G+ I+GG V   NP  T      + Y   NFN Y  GM T
Sbjct:  247 EQGKSIFTSYGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVAEKDRTRTRILKKAFNLIDNNNLGYLNKDQCIRLFEELNKYRTLPKISREDFELIFYELDDSHDVKINLDEFTDLSHAIGLRFQKEDSEPIFESCCPTFYHSYVSEKLKEFVRSP----KFGYVVAFILIMNFVAVVIETTLDIENNSAQEAWQKVEFVFGWLYVLEMALKVYAYGFVNYW-RDGQNRFDFI---ITWVIVIGETTTFLSPRGLTFLSNGEWIRYL-----LIARMLRLIRLLMHVQQYRAFVATFLTLIPSLMPYLGTIFCVMCIYCSLGVQIFGGIVNAGNPNLTGTDLSDDDYLLFNFNDYPNGMVT 628          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|697130047|ref|XP_009619087.1| (PREDICTED: two pore calcium channel protein 1A [Nicotiana tomentosiformis])

HSP 1 Score: 101.293 bits (251), Expect = 8.228e-19
Identity = 91/397 (22.92%), Postives = 161/397 (40.55%), Query Frame = 0

Query:  281 SGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPR---ALVIHVLR--RLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCY--------SPCAKRLMAFAVSAH--------LLVVVVSII-------------EFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
             G+  F ++      ++ L  T NNPDV++P YK    + + F  ++++  + + N+IL  +Y  +  +L      + + R R L  AF  +DE  +G L      L+   L   R  P+      ++I          +   D+F  L TA+  R  K  S+  +        SP +++L  F   A         LLV +V++I              FW+    V F      F  ++  ++  ++   G   Y+ +D  N  + V   ++ V ++          G++ L     ++ L     L A    + R+   +  Y A +  F  L+P+ +     I  ++  +C +G+ I+GG V   NP   +    GN Y   NFN Y  GM T
Sbjct:   97 QGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLFEELNKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQ---KVEF-----TFGWLYVIEMALKVYTYGFENYW-RDGQNRFDFV---VTWVIVIGETATFVAPDGLTFLSNGEWIRYL-----LIARMLRLIRLLMHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIVNTGNPNLAQTDLAGNDYLLFNFNDYPNGMVT 476          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|567909991|ref|XP_006447309.1| (hypothetical protein CICLE_v10014388mg [Citrus clementina] >gi|557549920|gb|ESR60549.1| hypothetical protein CICLE_v10014388mg [Citrus clementina])

HSP 1 Score: 101.679 bits (252), Expect = 8.511e-19
Identity = 92/396 (23.23%), Postives = 158/396 (39.90%), Query Frame = 0

Query:  281 SGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLR-----RLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCY--------SPCAKRLMAF--------AVSAHLLVVVVSII----------EFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYF--IKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
             G   F +F      ++ L  T NNPDV++P YK    + + F  F++I  + + N+IL  +Y  +  +L     +  + RRR L  AF  +D    G L +   I +       R  P   R + E I          +   D+F  L  A+  R  K     C+        SP +++L AF         +S  L+V +V++I               +  V F     +F  I+  ++  +I   G   Y+   ++RF+       L++ V ++   + L    G + L     ++ L     L A    + R+   + +Y   +  F  L+P+ +     I  +  ++C +G+ I+GG V   N    E     + Y   NFN Y  GM T
Sbjct:  210 QGNMVFTSFGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLIGVYFVTNLILAVVYDSFKSQLAKQVSEMDRMRRRTLGKAFNLIDNYNVGFLNKEQCIKLFEELNKYRTLPNISREEFELIFDELDDTHDFKINLDEFADLCNAIALRFQKEDVPSCFENLPSIYHSPFSEKLKAFIRSTKFGYMISIILIVNLVAVIVETTLDIQESSLQSVWQEVEF-----VFGWIYVLEMALKIYSYGFENYWRDGQNRFD------FLVTWVIVIGETITLASPNGQTFLSNGEWIRYL-----LLARMLRLIRLLMHVQQYRGFVATFLTLIPSLMPYLGTIFCVQCIYCSLGVQIFGGIVNAGNAKLEETDLADDDYLLFNFNDYPNGMVT 589          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|567909985|ref|XP_006447306.1| (hypothetical protein CICLE_v10014388mg [Citrus clementina] >gi|568877173|ref|XP_006491622.1| PREDICTED: two pore calcium channel protein 1 isoform X1 [Citrus sinensis] >gi|557549917|gb|ESR60546.1| hypothetical protein CICLE_v10014388mg [Citrus clementina])

HSP 1 Score: 102.064 bits (253), Expect = 8.709e-19
Identity = 92/396 (23.23%), Postives = 158/396 (39.90%), Query Frame = 0

Query:  281 SGQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLR-----RLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCY--------SPCAKRLMAF--------AVSAHLLVVVVSII----------EFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYF--IKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
             G   F +F      ++ L  T NNPDV++P YK    + + F  F++I  + + N+IL  +Y  +  +L     +  + RRR L  AF  +D    G L +   I +       R  P   R + E I          +   D+F  L  A+  R  K     C+        SP +++L AF         +S  L+V +V++I               +  V F     +F  I+  ++  +I   G   Y+   ++RF+       L++ V ++   + L    G + L     ++ L     L A    + R+   + +Y   +  F  L+P+ +     I  +  ++C +G+ I+GG V   N    E     + Y   NFN Y  GM T
Sbjct:  258 QGNMVFTSFGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLIGVYFVTNLILAVVYDSFKSQLAKQVSEMDRMRRRTLGKAFNLIDNYNVGFLNKEQCIKLFEELNKYRTLPNISREEFELIFDELDDTHDFKINLDEFADLCNAIALRFQKEDVPSCFENLPSIYHSPFSEKLKAFIRSTKFGYMISIILIVNLVAVIVETTLDIQESSLQSVWQEVEF-----VFGWIYVLEMALKIYSYGFENYWRDGQNRFD------FLVTWVIVIGETITLASPNGQTFLSNGEWIRYL-----LLARMLRLIRLLMHVQQYRGFVATFLTLIPSLMPYLGTIFCVQCIYCSLGVQIFGGIVNAGNAKLEETDLADDDYLLFNFNDYPNGMVT 637          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|1027852023|ref|NP_001311995.1| (two pore calcium channel protein 1A [Nicotiana tabacum] >gi|1025041854|ref|XP_016445355.1| PREDICTED: two pore calcium channel protein 1A [Nicotiana tabacum] >gi|75326539|sp|Q75VR1.1|TPC1A_TOBAC RecName: Full=Two pore calcium channel protein 1A; AltName: Full=Voltage-dependent calcium channel protein TPC1A; Short=NtTPC1A >gi|46275792|dbj|BAD15099.1| two-pore calcium channel [Nicotiana tabacum])

HSP 1 Score: 101.679 bits (252), Expect = 1.048e-18
Identity = 91/396 (22.98%), Postives = 161/396 (40.66%), Query Frame = 0

Query:  282 GQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPR---ALVIHVLR--RLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCY--------SPCAKRLMAFAVSAH--------LLVVVVSII-------------EFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYFIKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            G+  F ++      ++ L  T NNPDV++P YK    + + F  ++++  + + N+IL  +Y  +  +L      + + R R L  AF  +DE  +G L      L+   L   R  P+      ++I          +   D+F  L TA+  R  K  S+  +        SP +++L  F   A         LLV +V++I              FW+    V F      F  ++  ++  ++   G   Y+ +D  N  + V   ++ V ++          G++ L     ++ L     L A    + R+   +  Y A +  F  L+P+ +     I  ++  +C +G+ I+GG V   NP   +    GN Y   NFN Y  GM T
Sbjct:  247 GKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLFEELNKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQ---KVEF-----TFGWLYVIEMALKVYTYGFENYW-RDGQNRFDFV---VTWVIVIGETATFVAPDGLTFLSNGEWIRYL-----LIARMLRLIRLLMHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIVNTGNPNLAQTDLAGNDYLLFNFNDYPNGMVT 625          

BLAST of EWM27685.1 vs. NCBI_GenBank
Match: gi|567909989|ref|XP_006447308.1| (hypothetical protein CICLE_v10014388mg [Citrus clementina] >gi|557549919|gb|ESR60548.1| hypothetical protein CICLE_v10014388mg [Citrus clementina])

HSP 1 Score: 101.679 bits (252), Expect = 1.072e-18
Identity = 92/395 (23.29%), Postives = 158/395 (40.00%), Query Frame = 0

Query:  282 GQKAFENFAAAFVSLYSLSLTVNNPDVYLPYYKRGFLHIIVFASFLVITYFLIHNIILVRIYQIYCVKLKNNAVQRRQNRRRALCLAFEALDETKDGRLPRALVIHVLR-----RLWPRYDRAKIEAIMRHTHQYAPREYTFDQFEMLMTALNDRVSKHRSVRCY--------SPCAKRLMAF--------AVSAHLLVVVVSII----------EFWRFFYSVRFWVLISIFRAIFTADVIYRIKRMGGIKYF--IKDRFNVLNIVAILLSLVAIVAWCVGLYQDGGVSRLYPTSHMKKLAQLSVLFASCCDIARIFETIPEYNALLVVFGNLLPAFLGQAAVILTLMHVFCYIGMNIYGGKVEPTNPLWTEEPFPGNLYYTLNFNSYVEGMCT 643
            G   F +F      ++ L  T NNPDV++P YK    + + F  F++I  + + N+IL  +Y  +  +L     +  + RRR L  AF  +D    G L +   I +       R  P   R + E I          +   D+F  L  A+  R  K     C+        SP +++L AF         +S  L+V +V++I               +  V F     +F  I+  ++  +I   G   Y+   ++RF+       L++ V ++   + L    G + L     ++ L     L A    + R+   + +Y   +  F  L+P+ +     I  +  ++C +G+ I+GG V   N    E     + Y   NFN Y  GM T
Sbjct:  236 GNMVFTSFGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLIGVYFVTNLILAVVYDSFKSQLAKQVSEMDRMRRRTLGKAFNLIDNYNVGFLNKEQCIKLFEELNKYRTLPNISREEFELIFDELDDTHDFKINLDEFADLCNAIALRFQKEDVPSCFENLPSIYHSPFSEKLKAFIRSTKFGYMISIILIVNLVAVIVETTLDIQESSLQSVWQEVEF-----VFGWIYVLEMALKIYSYGFENYWRDGQNRFD------FLVTWVIVIGETITLASPNGQTFLSNGEWIRYL-----LLARMLRLIRLLMHVQQYRGFVATFLTLIPSLMPYLGTIFCVQCIYCSLGVQIFGGIVNAGNAKLEETDLADDDYLLFNFNDYPNGMVT 614