EWM27974.1, cds2687 (CDS) Nannochloropsis gaditana

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|585110315|gb|EWM27974.1| (ppgpp synthetase [Nannochloropsis gaditana])

HSP 1 Score: 1758.04 bits (4552), Expect = 0.000e+0
Identity = 855/855 (100.00%), Postives = 855/855 (100.00%), Query Frame = 0

Query:    1 MTSSTTRIKVLRRRPSGGGSMCRALPLLRAVVGYVTLLCLLLEPSSVAAFAPRGLSSQRCRTAVPVGSGTSQDMVIASSVATTASSSTGADPPRGMKARLPEHTLPPSSRLAAKKSRRPKLPTMYGLWDLVVGPVAADFKLVGMPSELTALAIEVHKATDHLTRPARLAVLQALVRSYHAYRFHKPCQITLDETGRRPSTTTAPQRAVAKALTVGAYMEQNVAVARLLGASEVDGDTMVAALLCHSPLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIERGQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDFVKASCLQPKAGTAQGKRGTRNKRVEEGRGCGRLHPQGLWRDDSAHEEHGGYQTVPVSRRKSKRSTQGMLLLNGQSAPALDPPTHVKSVNIAKTGSSCKISGPFVVGRRRAKSISKAPRDFFLSKRGPFCTRRLILLEGQRLKVYRVKSIDLRSLRGLCNAALRSADMLCLVTSVYVGPLHYEIRPRNTHRSRKIAASVFESK 855
            MTSSTTRIKVLRRRPSGGGSMCRALPLLRAVVGYVTLLCLLLEPSSVAAFAPRGLSSQRCRTAVPVGSGTSQDMVIASSVATTASSSTGADPPRGMKARLPEHTLPPSSRLAAKKSRRPKLPTMYGLWDLVVGPVAADFKLVGMPSELTALAIEVHKATDHLTRPARLAVLQALVRSYHAYRFHKPCQITLDETGRRPSTTTAPQRAVAKALTVGAYMEQNVAVARLLGASEVDGDTMVAALLCHSPLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIERGQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDFVKASCLQPKAGTAQGKRGTRNKRVEEGRGCGRLHPQGLWRDDSAHEEHGGYQTVPVSRRKSKRSTQGMLLLNGQSAPALDPPTHVKSVNIAKTGSSCKISGPFVVGRRRAKSISKAPRDFFLSKRGPFCTRRLILLEGQRLKVYRVKSIDLRSLRGLCNAALRSADMLCLVTSVYVGPLHYEIRPRNTHRSRKIAASVFESK
Sbjct:    1 MTSSTTRIKVLRRRPSGGGSMCRALPLLRAVVGYVTLLCLLLEPSSVAAFAPRGLSSQRCRTAVPVGSGTSQDMVIASSVATTASSSTGADPPRGMKARLPEHTLPPSSRLAAKKSRRPKLPTMYGLWDLVVGPVAADFKLVGMPSELTALAIEVHKATDHLTRPARLAVLQALVRSYHAYRFHKPCQITLDETGRRPSTTTAPQRAVAKALTVGAYMEQNVAVARLLGASEVDGDTMVAALLCHSPLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIERGQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDFVKASCLQPKAGTAQGKRGTRNKRVEEGRGCGRLHPQGLWRDDSAHEEHGGYQTVPVSRRKSKRSTQGMLLLNGQSAPALDPPTHVKSVNIAKTGSSCKISGPFVVGRRRAKSISKAPRDFFLSKRGPFCTRRLILLEGQRLKVYRVKSIDLRSLRGLCNAALRSADMLCLVTSVYVGPLHYEIRPRNTHRSRKIAASVFESK 855          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|502921949|ref|WP_013156925.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Meiothermus silvanus] >gi|296849303|gb|ADH62318.1| (p)ppGpp synthetase I, SpoT/RelA [Meiothermus silvanus DSM 9946])

HSP 1 Score: 193.741 bits (491), Expect = 3.441e-48
Identity = 148/451 (32.82%), Postives = 233/451 (51.66%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAH-------FLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLE-DPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAVA +L    +D +T++A LL  +     +  E +E  FG AV  I         VEG+ ++  L ++ AH        L   + ++LR + + +A+D R + +++A +   + T  F    ++       L +YA LA   G+ QIK  LE+ +F++L P+ +  ++  L++ R   +  + +  KE L+A+L+ D V  ++++  EV GR K  YS+WKKM+R+              G  LE +YD++ALRV+  P                   PTP  L+ +A  +KQ+C +VL  +VH L     GRVKDY+     K NGYQSLHTT+   + G   P+E+QIRT EMH +AE+G+AAH LYK   T  E I+R     ++ Q+ Q E         F+   EF+    + L    V VF+P G+++ L RGA+ +DF
Sbjct:   53 YIVHPVAVAEILAELGLDAETLMAGLLHDTVEDTEVKPEDIEERFGTAVRRI---------VEGETKVSKL-YKLAHQVAEERPSLEEQRAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPHKQTRIARETLEIYAPLAHRLGIGQIKWELEDLSFRYLEPEAYADLLGRLKSHRAEREAAVERG-KEALEAVLQRDSVLGASIQGYEVTGRTKHLYSIWKKMQRE--------------GRALEQIYDLLALRVILEPR------------------PTP-NLEEKAMREKQVCYHVL-GLVHALWQPIPGRVKDYIAVP--KPNGYQSLHTTV---IAGSGLPLEVQIRTREMHQIAEYGIAAHWLYKQGLTDPEEIKRRVDWLRSIQEWQAE---------FSSSREFVDAVTRDLLGGRVFVFTPKGRIINLPRGASPVDF 444          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|503222356|ref|WP_013457017.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Oceanithermus profundus] >gi|313151996|gb|ADR35847.1| (p)ppGpp synthetase I, SpoT/RelA [Oceanithermus profundus DSM 14977])

HSP 1 Score: 193.741 bits (491), Expect = 3.582e-48
Identity = 141/442 (31.90%), Postives = 224/442 (50.68%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS-----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIERGQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAV  +L   ++D D+++A LL  +      ++ E +   FG+ V  I         VEG+ ++  L  + A  +   Q +NLR + + + +D R + +++A +   M T  F   +++       L ++A +A   G+ QIK  LE+ AF++L P+E+  ++  L      +++V+  A+  +  AL  D V   +V+   V GR K  +S+WKKM+R+                 LE +YD++ALRV+  P               PV+ P  A     A  +KQ+C +VL  +VH L P   GRVKDY+ +   K NGYQSLHTT+   +     P+E+QIRT EMH VAE+G+AAH LYK   T    ++R  +  E  +QE       QR F    +F+   Q  L    V VF+P G+++ L +G+T +DF
Sbjct:   52 YITHPVAVTEILAELQMDPDSLIAGLLHDTVEDVESVSFETIRQRFGEDVARI---------VEGETKVSKL-SKMATSVEDQQAENLRQMFIAMTRDLRIIIVKLADRTHNMRTLEFMPPEKQRRISKETLEIFAPMAHRLGIGQIKLELEDLAFRYLHPEEYAALVERLAHHSQAHREVVDNAKGALEHALENDYVLGLSVDGFSVSGRTKHLFSIWKKMQRE--------------EKTLEQIYDLLALRVILDP--------------KPVASPEEA-----ASREKQVCYHVL-GLVHALWPPIPGRVKDYIAQP--KPNGYQSLHTTV---ITPGGLPLEVQIRTREMHRVAEYGVAAHWLYKEGLTDPNQLKRRVKWIE-LLQEW------QRDFTTSRDFVEAVQHDLLSGRVFVFTPKGRVIDLPKGSTPVDF 437          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|545706020|ref|XP_005705328.1| (guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Galdieria sulphuraria] >gi|452821782|gb|EME28808.1| guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Galdieria sulphuraria])

HSP 1 Score: 193.741 bits (491), Expect = 4.575e-48
Identity = 150/456 (32.89%), Postives = 224/456 (49.12%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIERGQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDG-------KLLKLNRGATLLD--FVKASCL 659
            ++   VAVA +L    +D DT+VA LL  +     +TL  +E  FG  V  I         VEG+ ++  L  + A      Q +NLR L + + +DWR + +++A +   M T  F  + +        L+++A LA   GM QIK  LE   F +L P E+ ++   +E K  +Y +VL ++R+ +  AL  D +   +V +VEV  RIK  YS+WKKM++               G  +E +YD+VALRV+ TP     E  S+ +     S       +    L+K +C Y L  +VH +   +  RVKDY+     K NGYQSLHTT+     G   P+E+QIRT EMH VAE+G+AAH +YK +     VI     +    + E  D I   R      EF+   ++ L  S V VF+          ++  L RG+T++D  F   SCL
Sbjct:  128 FITHPVAVANILAELRMDRDTIVAGLLHDTVEDTEVTLSTIEQLFGMEVRRI---------VEGETKVSKL-PKMADISIDKQAENLRQLFIAMTEDWRIIIVKLADRLHNMRTLQFMKQAKRHKIAKETLDIFAPLAHRLGMWQIKAELERLGFMYLYPSEYEEIRQLVEKKLPKYMRVLEESRQALKTALQNDVILQQSVAQVEVSARIKELYSLWKKMQQH--------------GKDIEDIYDLVALRVLITPKPMQLECCSSDA-----SQCNEWNRKMLETLEKSLCYYTLG-IVHAMWQPFPCRVKDYIA--FPKENGYQSLHTTVM--AGGAQAPLEVQIRTLEMHLVAEYGMAAHWMYKDNSV---VIASKATRWMSTIVEWGDEIANSR------EFVELVRRELLGSRVFVFAKFESYSDRCLEIFNLPRGSTIVDIAFQLDSCL 540          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|503470115|ref|WP_013704776.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Marinithermus hydrothermalis] >gi|328451830|gb|AEB12731.1| (p)ppGpp synthetase I, SpoT/RelA [Marinithermus hydrothermalis DSM 14884])

HSP 1 Score: 192.971 bits (489), Expect = 5.967e-48
Identity = 149/443 (33.63%), Postives = 229/443 (51.69%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQGDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            ++   VAVA +L   ++D DT++A LL  +     +TL+A+E  FG  V  I         VEG+ ++  L  + A  +   Q +NLR + + + +D R + +++A +   M T  F   +++       L ++A LA   G+ QIK  LE+ AF++L P+E   +   L   R  +Q ++ +A+  + +AL +D V    V+   V GR K  YS+WKKM+R               G  LE +YD++ALRV+  P               P S P  A  +AR   +KQ+C +V+  +VH L     GRVKDY+     K NGYQSLHTT+   +  +  P+E+QIRT EMH +AE+G+AAH LYK   T  E ++R     +A Q+ Q+E         F+   EF+    K L    V VF+P G+++ L +GAT +DF
Sbjct:   52 FITHPVAVAGILAELKMDEDTLIAGLLHDTVEDTDVTLDAIEARFGPTVRRI---------VEGETKVSKL-PKIAESIEDEQAENLRQMFIAMTEDVRIIIVKLADRLHNMRTLEFMPPEKQQRISRETLEIFAPLAHRLGIGQIKWELEDLAFRYLHPEECAALEVRLSHHRAEHQAIVERAKAALEEALAKDYVLKLAVQEYTVTGRTKHLYSIWKKMER--------------DGKTLEQIYDLLALRVILDP--------------RPSSDPEEA--KAR---EKQVCYHVI-GLVHALWQPIPGRVKDYIAMP--KPNGYQSLHTTV---ITWQGMPLEVQIRTREMHRIAEYGIAAHWLYKEGLTNPEEVKRRVSWLRAIQEWQQE---------FSSSREFVEAVTKDLLGGRVFVFTPKGQIINLPKGATPVDF 436          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|927054342|ref|WP_053767443.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Thermus aquaticus] >gi|926448983|gb|KOX89664.1| GTP pyrophosphokinase [Thermus aquaticus])

HSP 1 Score: 191.045 bits (484), Expect = 2.213e-47
Identity = 143/445 (32.13%), Postives = 236/445 (53.03%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQ--GDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAVA +L +  +D DT+ A LL  +     +  E +E  FG AV  I         VEG+ ++  L +++A+ L G +   ++LR + + +A+D R + +++A +   + T     + ++       L +YA LA   GM QIK  LE+ +F++L P+ ++ ++  L+  ++  ++++ +A + + +AL +D +  S ++  EV GR K  YS+WKKM+R+              G  LE +YD++A+RV+  P                   P+P T +A+   +KQ+C +VL  +VH L     GRVKDY+     K NGYQSLHTT+   +  E  P+E+QIRT EMH +AE+G+AAH LYK   T  E ++R     +  Q+ Q+E         F+   EF+    + L    V VF+P G+++ L +GAT +DF
Sbjct:   48 YITHPVAVAEILASLRMDADTVAAGLLHDTMEDCGVAGEELERRFGPAVRRI---------VEGETKVSKL-YKFAN-LEGEEKRAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEHMPEGKQKRIAQETLEIYAPLAHRLGMGQIKWELEDLSFRYLHPEAYQALLARLQETQEARERMVKRAMEVLEEALKQDALLQSQLQGFEVTGRPKHLYSIWKKMERE--------------GKALEQIYDLLAVRVILDP------------------KPSP-TEEAKGLREKQVCYHVL-GLVHALWQPIPGRVKDYIAVP--KPNGYQSLHTTV---IALEGLPLEVQIRTREMHRIAEYGIAAHWLYKEGLTDPEEVKRRVSWLKNIQEWQQE---------FSSSREFVEAVTRDLLGGRVFVFTPKGRIINLPKGATPVDF 433          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|489137290|ref|WP_003047072.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Thermus aquaticus] >gi|218243478|gb|EED10007.1| (p)ppGpp synthetase I, SpoT/RelA [Thermus aquaticus Y51MC23] >gi|939576104|gb|ALJ90012.1| GTP pyrophosphokinase (pppGpp) synthetase II [Thermus aquaticus Y51MC23])

HSP 1 Score: 190.66 bits (483), Expect = 3.781e-47
Identity = 143/445 (32.13%), Postives = 236/445 (53.03%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQ--GDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAVA +L +  +D DT+ A LL  +     +  E +E  FG AV  I         VEG+ ++  L +++A+ L G +   ++LR + + +A+D R + +++A +   + T     + ++       L +YA LA   GM QIK  LE+ +F++L P+ ++ ++  L+  ++  ++++ +A + + +AL +D +  S ++  EV GR K  YS+WKKM+R+              G  LE +YD++A+RV+  P                   P+P T +A+   +KQ+C +VL  +VH L     GRVKDY+     K NGYQSLHTT+   +  E  P+E+QIRT EMH +AE+G+AAH LYK   T  E ++R     +  Q+ Q+E         F+   EF+    + L    V VF+P G+++ L +GAT +DF
Sbjct:   48 YITHPVAVAEILASLRMDADTVAAGLLHDTMEDCGVAGEELERRFGPAVRRI---------VEGETKVSKL-YKFAN-LEGEEKRAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEHMPEGKQKRIAQETLEIYAPLAHRLGMGQIKWELEDLSFRYLHPEAYQALLARLQETQEARERMVKRAMEVLEEALKQDALLQSQLQGFEVTGRPKHLYSIWKKMERE--------------GKALEQIYDLLAVRVILDP------------------KPSP-TEEAKGLREKQVCYHVL-GLVHALWQPIPGRVKDYIAVP--KPNGYQSLHTTV---IALEGLPLEVQIRTREMHRIAEYGIAAHWLYKEGLTDPEEVKRRVSWLKNIQEWQQE---------FSSSREFVEAVTRDLLGGRVFVFTPKGRIINLPKGATPVDF 433          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|917553993|ref|WP_052155196.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Thermus sp. 2.9])

HSP 1 Score: 190.66 bits (483), Expect = 3.853e-47
Identity = 144/446 (32.29%), Postives = 235/446 (52.69%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHSPL-----TLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQ--GDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAVA +L +  +D DT+ A LL H  L       E +E  FG AV  I         VEG+ ++  L ++ A+ L G +   ++LR + + +A+D R + +++A +   + T  F   +++       L +YA LA   GM Q+K  LE+ +F++L P+ ++ +++ ++  ++  ++++ +A   + +AL  D +  + ++  EV GR K  YS+WKKM+R+                 LE +YD++A+RV+  P                   P+P T + RA  +KQ+C +VL  +VH L     GRVKDY+     K NGYQSLHTT+   +  E  P+E+QIRT EMH +AE+G+AAH LYK   T  E I+R     ++ Q+ Q+E         F+   EF+    + L    V VF+P G+++ L +GAT +DF
Sbjct:   48 YITHPVAVAEILASLRMDADTVAAGLL-HDTLEDCGVAAEELERRFGPAVRRI---------VEGETKVSKL-YKLAN-LEGEEKRAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPEKQKRIAQETLEIYAPLAHRLGMGQLKWELEDLSFRYLHPEAYQALLSRIQETQEARERLVKKAMAALEEALKRDELLQAQLQGFEVTGRPKHLYSIWKKMERE--------------KKALEQIYDLLAVRVILDP------------------KPSP-TEEGRALREKQVCYHVL-GLVHALWQPIPGRVKDYIA--VPKPNGYQSLHTTV---IALEGLPLEVQIRTREMHRIAEYGIAAHWLYKEGLTDPEEIKRRVSWLKSIQEWQQE---------FSSSREFVEAVTRDLLGGRVFVFTPKGRIINLPKGATPVDF 433          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|729023109|gb|KHG64853.1| (GTP pyrophosphokinase [Thermus sp. 2.9])

HSP 1 Score: 190.274 bits (482), Expect = 3.970e-47
Identity = 144/446 (32.29%), Postives = 235/446 (52.69%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHSPL-----TLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQ--GDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAVA +L +  +D DT+ A LL H  L       E +E  FG AV  I         VEG+ ++  L ++ A+ L G +   ++LR + + +A+D R + +++A +   + T  F   +++       L +YA LA   GM Q+K  LE+ +F++L P+ ++ +++ ++  ++  ++++ +A   + +AL  D +  + ++  EV GR K  YS+WKKM+R+                 LE +YD++A+RV+  P                   P+P T + RA  +KQ+C +VL  +VH L     GRVKDY+     K NGYQSLHTT+   +  E  P+E+QIRT EMH +AE+G+AAH LYK   T  E I+R     ++ Q+ Q+E         F+   EF+    + L    V VF+P G+++ L +GAT +DF
Sbjct:   49 YITHPVAVAEILASLRMDADTVAAGLL-HDTLEDCGVAAEELERRFGPAVRRI---------VEGETKVSKL-YKLAN-LEGEEKRAEDLRQMFIAMAEDVRIIIVKLADRLHNLRTLEFMPPEKQKRIAQETLEIYAPLAHRLGMGQLKWELEDLSFRYLHPEAYQALLSRIQETQEARERLVKKAMAALEEALKRDELLQAQLQGFEVTGRPKHLYSIWKKMERE--------------KKALEQIYDLLAVRVILDP------------------KPSP-TEEGRALREKQVCYHVL-GLVHALWQPIPGRVKDYIA--VPKPNGYQSLHTTV---IALEGLPLEVQIRTREMHRIAEYGIAAHWLYKEGLTDPEEIKRRVSWLKSIQEWQQE---------FSSSREFVEAVTRDLLGGRVFVFTPKGRIINLPKGATPVDF 434          

BLAST of EWM27974.1 vs. NCBI_GenBank
Match: gi|654411577|ref|WP_027883090.1| (bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [Meiothermus rufus])

HSP 1 Score: 190.274 bits (482), Expect = 5.053e-47
Identity = 142/449 (31.63%), Postives = 228/449 (50.78%), Query Frame = 0

Query:  217 YMEQNVAVARLLGASEVDGDTMVAALLCHS----PLTLEAVENEFGKAVGTILAGWKAMLGVEGQLEIKSLIHEYAHFLWGGQ------GDNLRNLLLVVAKDWRAVALRVAWKKVEMETTVFGAKKEEIDACWACLNVYASLAEAAGMSQIKNALEERAFKFLMPQEHRKVMTALEAKRDRYQKVLGQARKEVLQALLEDPVFMSNVERVEVRGRIKSPYSVWKKMKRKGMLVEEGEGGGEGSGMGLEGVYDMVALRVVCTPLYADKEASSASSATLPVSPPTPATLQARADLDKQMCDYVLREVVHKLRPHYKGRVKDYVGEENRKANGYQSLHTTLAYRLRGETTPVEIQIRTSEMHSVAEHGLAAHCLYKGDRTLAEVIER--GQQQAEQQMQEEEDAIGPQRGFAGPGEFLTWCQKMLDKSHVIVFSPDGKLLKLNRGATLLDF 653
            Y+   VAVAR+L    +D +T++A LL  +     +  E +E  FG AV  I         VEG+ ++  L ++ AH     +       ++LR + + +A+D R + +++A +   + T  F   +++       L +YA LA   G+ Q+K  LE+ +F +L P+ ++ +   L++ R   +  +  AR++++QAL  D +   +++R EV GR K  YS+WKKM+R+              G  LE +YD++ALRV+  P                 S P    L+      KQ+C +VL  +VH +     GRVKDY+     K NGYQSLHTT+   +     P+E+QIRT EMH VAE G+AAH LYK   T  E ++R  G  +  Q+ Q+E         ++   +F+    + L    V VF+P GK++ L +GA+ +DF
Sbjct:   45 YITHPVAVARILAELGLDAETLMAGLLHDTIEDTEVRPEELEERFGPAVRKI---------VEGETKVSKL-YKLAHAAAEDKPAEERHAEDLRQMFIAMAEDVRIIVVKLADRLHNLRTLEFMPPEKQRRIARETLEIYAPLAHRLGIGQVKLELEDLSFCYLEPERYQDLEARLKSHRAEREAAVQDAREKLIQALQRDFILRQSIQRFEVTGRTKHLYSIWKKMQRE--------------GKALEQIYDLLALRVILEPRQG--------------SDPEENALRG-----KQVCYHVL-GLVHAIWQPIPGRVKDYIAVP--KPNGYQSLHTTV---ISVNGLPLEVQIRTKEMHQVAEFGIAAHWLYKEGVTDPEELKRRVGWLKNIQEWQQE---------YSSSRDFVEAVTRELLGGRVFVFTPKGKIINLPKGASPVDF 435