EWM28099.1, cds2579 (CDS) Nannochloropsis gaditana

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|585110442|gb|EWM28099.1| (iduronate-2-sulfatase [Nannochloropsis gaditana])

HSP 1 Score: 1447.18 bits (3745), Expect = 0.000e+0
Identity = 690/690 (100.00%), Postives = 690/690 (100.00%), Query Frame = 0

Query:    1 MNMFASQSMHQGQHENLTYHTKFPKCVTAHPLPFPGNFLIRMFPRSGRPTGRPIGQEHTRNFNPSGRNTVVLVILVIMGLSNVYLYLGPTHSRRGIFTTDSSVNDGFSEANEHKLSARHPRSRPQLVDDPIVKEDTPSLAYPYAGRGNPFSSHKTRDRTKRKNVLFIMTDDLRPSLSIYNKPVITPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFEDTVASDYGNIFRYFKKEGGYKMLGTGKLWHWTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRSIVDTYNYKQWKLKSQHQSAKKVAEQRLRLQ 690
            MNMFASQSMHQGQHENLTYHTKFPKCVTAHPLPFPGNFLIRMFPRSGRPTGRPIGQEHTRNFNPSGRNTVVLVILVIMGLSNVYLYLGPTHSRRGIFTTDSSVNDGFSEANEHKLSARHPRSRPQLVDDPIVKEDTPSLAYPYAGRGNPFSSHKTRDRTKRKNVLFIMTDDLRPSLSIYNKPVITPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFEDTVASDYGNIFRYFKKEGGYKMLGTGKLWHWTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRSIVDTYNYKQWKLKSQHQSAKKVAEQRLRLQ
Sbjct:    1 MNMFASQSMHQGQHENLTYHTKFPKCVTAHPLPFPGNFLIRMFPRSGRPTGRPIGQEHTRNFNPSGRNTVVLVILVIMGLSNVYLYLGPTHSRRGIFTTDSSVNDGFSEANEHKLSARHPRSRPQLVDDPIVKEDTPSLAYPYAGRGNPFSSHKTRDRTKRKNVLFIMTDDLRPSLSIYNKPVITPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFEDTVASDYGNIFRYFKKEGGYKMLGTGKLWHWTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRSIVDTYNYKQWKLKSQHQSAKKVAEQRLRLQ 690          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|1198829889|ref|WP_086949091.1| (iduronate-2-sulfatase [Sphingobacterium sp. JB170] >gi|1145436089|emb|SJN46790.1| Choline-sulfatase [Sphingobacterium sp. JB170])

HSP 1 Score: 266.159 bits (679), Expect = 1.173e-77
Identity = 185/510 (36.27%), Postives = 261/510 (51.18%), Query Frame = 0

Query:  159 TKRKNVLFIMTDDLRPSLSIYNKPVITPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFED---TVASDYGNIFRYFKKEGGYKMLGTGKLWH----------WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLE-IGMLPDSEKTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDP--DIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKK 652
             +R NVLFI  DDLR +    N   ITP  +RLAK GVVF R Y Q  +C PSR S+L+GRRPD TKVW  +        D   + +YFK+ G Y     GK++H          W++  I+   +   PK     N  T+ +++  +         P+  + D ++AS  ++++RE    KD + FFL VGF +PH P+  P++YWDLY    +P     + P  AP +A  D   L     +PDS         G +P   I++   GY+ASIS++D QI RL+DELE+  L+E+T+IV  +DHG ++G+ G+W K T FE + RVPLII  P   + ES   N  AL E +DI+PT+ E+AGL  P G     LEGKS+VP+L++P                       RP KK  +      S   R W   NE  YMGYS+RT E+RYT WV  +          A   +ELY H  P   EK N    +     +   ++L+++
Sbjct:   29 ARRPNVLFIAVDDLRSNDG--NFAAITPNIDRLAKKGVVFNRAYCQQAVCGPSRASVLTGRRPDVTKVWDLKTHFRQALPDAVTLPQYFKQHG-YAARNVGKIYHDSKEAQDPVSWSAPEIKAITDAAGPKYVLPENQPTNGSWKKASWECL---EVPDTAYVDGQVASAAVNVIRE---LKDSS-FFLAVGFRRPHLPFSAPKRYWDLYNQSEIPPPSPDSAPEAAPKIALHDGVELRGYNDIPDS---------GAIPVTKIQQLRHGYYASISYIDAQIGRLIDELEQQGLMENTVIVLWSDHGFHLGELGLWAKTTNFEMDTRVPLIICSPHVGVKES---NTEALVELIDIYPTLAELAGLDIPDG-----LEGKSMVPLLKNP----------------------TRPWKKAAF------SQFPRPWMYKNEPKYMGYSVRTKEYRYTEWVNLDEG--------AVESKELY-HYSPSTLEKRNLAGSKEHRRIEKSMMKLLRQ 474          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|1133492910|ref|XP_003391778.2| (PREDICTED: iduronate 2-sulfatase-like [Amphimedon queenslandica])

HSP 1 Score: 255.373 bits (651), Expect = 5.389e-73
Identity = 182/539 (33.77%), Postives = 276/539 (51.21%), Query Frame = 0

Query:  155 TRDRTKRKNVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFED-----TV--ASDYGNIFRYFKKEGGYKMLGTGKLWH-WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTK---DGNPFFLGVGFHQPHRPWHMPQKYWDLY---EDRYVPVTPHTTWPIDAPGVA---------TGDINPLEIGMLPDSE-KTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDP------AGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERV--YMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRS 660
            T   + +KNVLF   DDLRP L+ Y    I +P  + LA   ++FER Y Q  +C+PSR SLL+GRRPDT  VW         TV  A++   I +YFK E GY  +G GK++H    S  +++   + P+    ++   D +     A    D+  P+ +  D +IA   ++++++    +   D  PFF+ VGFH+PH P++   KY+DLY   ED  +P  P    P   P VA           DI  +    L D++ +  +      VP + ++E  R Y++ +SF D QI R++ EL+     +DTIIV   DHG  +G+H  W K T FE    VP ++  P + ++ GM   AL E +DIFP++ E+AGL  P      +      +EG S+VP+L +P N + K  + +Q PR  +QG    P K  P F             D +E++   MGY++RTD++R+T W  FN +T TP +   W G ELYDH  P       F + E  N+A +  ++ V  EL   +++
Sbjct:   16 TSGASGKKNVLFFAVDDLRPQLNAYGVDFIKSPNIDALASKSMLFERAYCQIAVCSPSRASLLTGRRPDTNHVWQIAADEYWRTVPDATNATTIPQYFK-ENGYLSIGMGKIFHPGPPSGYDDYKYSWSPEGLPYFHGKEDSSIAKHAAWYSFDE--PDNKRVDGQIADNAVNVLQQIKKNRTEGDDRPFFVAVGFHKPHLPFYCSSKYYDLYPSIEDTKLPENPDV--PKGMPDVAFTIWKELKDYSDIKAIINETLCDTDAEASIYGKECHVPDNKMKELRRAYYSCVSFTDAQIGRVIKELDAQGFADDTIIVLWGDHGWQLGEHNQWGKCTNFEDATHVPFLLHVPGVTDN-GMTTKALVELIDIFPSLTELAGLEVPPMCTKNSPTSIACVEGSSVVPLLSNP-NAQWKKGSFSQYPRPSTQGLPEIPGK--PPF-------------DPSEKLESVMGYTVRTDKYRFTEWYKFNRNTSTPNFNDIW-GTELYDHSSPTV-----FFNDENVNLATESEMKQVVDELRQMLQA 526          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|1057424180|ref|WP_068770069.1| (hypothetical protein [Opitutaceae bacterium TSB47])

HSP 1 Score: 254.218 bits (648), Expect = 6.351e-73
Identity = 182/538 (33.83%), Postives = 254/538 (47.21%), Query Frame = 0

Query:  163 NVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVW----LFEDTVASDYGNIFRYFKKEGGYKMLGTGKLWH--------WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQP--DDNWPEERFWDYRIASEGISLMREAVNTKDGN--PFFLGVGFHQPHRPWHMPQKYWDLY--EDRYVPV---------TPH--TTWPIDAPGVATGDINPLEIGMLPDSEKTYVADP-TGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRSIVDTYNYKQ 669
            NVLFI++DDL+P L  Y  PV+ TP  +RLA+ G+VF+R Y Q  +C+PSR SLL+GRRPDTTKV+     F + +  D   + +YFK+ G Y     GK++H        WT     +   +Y P + +       EN      +  P    + P+    D  +A+E +  +      +  N  PFFL VGF  PH P+  P+KYWD+Y  ED  +P          TP+  TTW                        + Y   P TG +  +  R  + GY+A +S+LD QI RLLD LE+  L E+TI+V   DHG ++GDHG W K T FE     PLII  P   ++ G    AL E VD++PT+ E+ GLP PAG      EGKSLVP++  P   +    A +Q  R                      SGG            MGYS+RTD  RYT W+  NA T            ELYDH           D+ E  N+A       + ++L+A++ +++     K+
Sbjct:   35 NVLFIVSDDLKPVLGCYGDPVVRTPNIDRLAERGLVFQRAYAQQAVCSPSRTSLLTGRRPDTTKVYDLVTHFREAI-PDVVTLPQYFKQHG-YTTQSLGKIFHPGYDDPQSWTIQNTFKAAARYSPPAREILAKRLAENKPGAKIAGLPWESPDVPDNSLTDGSVATEAVKTLGSLAQARRQNDQPFFLAVGFLVPHLPFSAPKKYWDIYCPEDVRLPENYRRPTAGETPYSRTTW---------------------SELRKYAGIPATGPLTDEQARSMIHGYYACVSYLDAQIGRLLDALEQQGLAENTIVVLWGDHGWHLGDHGQWTKHTNFEQATHAPLIISAPGA-KTAGQKTRALVEFVDVYPTLAELCGLPAPAG-----AEGKSLVPLIESPAR-KWAPAAFSQYLR----------------------SGG-----------IMGYSIRTDRHRYTEWLGPNAETL---------ARELYDHET---------DATEDYNIAGRAEHAALVQQLHAQLEAVLHISTIKR 491          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|1099560252|emb|SFH83305.1| (iduronate 2-sulfatase [Parapedobacter indicus])

HSP 1 Score: 254.218 bits (648), Expect = 6.522e-73
Identity = 187/564 (33.16%), Postives = 278/564 (49.29%), Query Frame = 0

Query:  139 LAYPYAGRGNPFSSHKTRDR-TKRKNVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFEDTVASDYGNIF---RYFKKEGGYKMLGTGKLWH----------WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADP-TGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRSIVDTYNYKQWKLKSQHQSAKKVAEQR 686
            LA P  G      + K R + + R NVLFI  DDLRP L  Y   V  TP  +RLAK G+VF R Y Q  +C PSR S+L+GRRPD TKVW  +    +   ++    ++FK++G Y     GK++H          W++  I    ++  PK     N  T  +++   A+     + P+  + D ++ +  + L+ E  +T     FFL VGF +PH P+  P++YWDLY+   +P+  H   P + P +A    N +E+       + Y   P  G + S  IRE   GY+AS+S++DHQI RLLDELER +L+ +TIIV  +DHG ++G+  +W K T FE + RVPLII  P       ++  AL E VD++PT+ ++AGLP   G     LEG S+ P++ +P                      NR  KK       V S   R W    E   MGYS+RT ++RYT W+  N         ++    ELY +           +  ET N        LV +  Y+K ++++    +  WK K++  +A K A  +
Sbjct:   16 LALPTTGISVAQVNEKARSKDSSRPNVLFIAVDDLRPQLGCYGDTVAHTPNIDRLAKIGIVFNRAYCQQAVCAPSRASVLTGRRPDATKVWDLKTHFRTALPHVVTLPQHFKQQG-YDTRNVGKIYHDPKNAQDPISWSAPEIMAVTDEAGPKYVLTENLPTAGSWK---AAATECVDVPDSAYVDGKVGNAAVELIEELKDTT----FFLAVGFRRPHLPFTAPKRYWDLYDVDEIPLPAHDRAPENTPEIALH--NSVEL-------RGYTDIPDVGPMSSGKIRELRHGYYASVSYIDHQIGRLLDELERQNLLANTIIVLWSDHGYHLGELDLWSKTTNFELDTRVPLIIASPLFHHQH-VHTDALVELVDLYPTLADLAGLPITDG-----LEGTSMAPLIANP----------------------NRTWKKA------VFSQFPRPWMYQGEPEVMGYSVRTADYRYTEWISTN--------DKSVKAAELYHYN----------EGIETAN--------LVDQATYSKKQAVLRRLLHDGWK-KARPGNAGKSASSK 501          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|340378457|ref|XP_003387744.1| (PREDICTED: iduronate 2-sulfatase-like [Amphimedon queenslandica])

HSP 1 Score: 254.218 bits (648), Expect = 1.188e-72
Identity = 182/539 (33.77%), Postives = 276/539 (51.21%), Query Frame = 0

Query:  155 TRDRTKRKNVLFIMTDDLRPSLSIYNKPVIT-PGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFED-----TV--ASDYGNIFRYFKKEGGYKMLGTGKLWH-WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTK---DGNPFFLGVGFHQPHRPWHMPQKYWDLY---EDRYVPVTPHTTWPIDAPGVA---------TGDINPLEIGMLPDSE-KTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDP------AGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERV--YMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRS 660
            T   + +KNVLF   DDLRP L+ Y    IT P  + LA   ++FER Y Q  +C+PSR SLL+GRRPDT  VW         TV  A++   I +YFK E GY  +G GK++H    S  +++   + P+    ++   D +     A    D+  P+ +  D +IA   ++++++    +   D  PFF+ VGFH+PH P++   KY+DLY   ED  +P  P    P   P VA           DI  +    L D++ +  +      VP + ++E  R Y++ +SF D QI R++ EL+     +DTII+   DHG  +G+H  W K T FE    VP ++  P + ++ GM   AL E +DIFP++ E+AGL  P      +      +EG S+VP+L +P N + K  + +Q PR  +QG    P K  P F             D +E++   MGY++RTD++R+T W  FN +T TP +   W G ELYDH  P       F + E  N+A +  ++ V  EL   +++
Sbjct:   16 TSGASGKKNVLFFAVDDLRPQLNAYGVDFITSPNIDALASKSMLFERAYCQIAVCSPSRASLLTGRRPDTNHVWQIAADEYWRTVPDATNATTIPQYFK-ENGYLSIGMGKIFHPGPPSGYDDYKYSWSPEGRPYFHAKEDSSIAKGAAWYSFDE--PDNKRVDGQIADNAVNVLQQIKKNRTEGDDRPFFVAVGFHKPHLPFYCSSKYYDLYPSIEDTKLPENPDV--PKGMPDVAFTIWKELKDYSDIKAIINETLCDTDAEASIYGKECHVPDNKMKELRRAYYSCVSFTDAQIGRVIKELDAQGFADDTIIILWGDHGWQLGEHNQWGKCTNFEDATHVPFLLHVPGVTDN-GMTTKALVELIDIFPSLTELAGLEVPPMCTKNSPTSIACVEGSSVVPLLSNP-NAQWKKGSFSQYPRPSTQGLPEIPGK--PPF-------------DPSEKLESVMGYTVRTDKYRFTEWYKFNRNTSTPNFNDIW-GTELYDHSSPTV-----FFNDENVNLATESEMKPVVDELRQLLQA 526          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|1154908668|ref|WP_078814517.1| (iduronate sulfatase [Prosthecobacter debontii] >gi|1152227055|emb|SKB01198.1| iduronate 2-sulfatase [Prosthecobacter debontii])

HSP 1 Score: 252.677 bits (644), Expect = 1.303e-72
Identity = 179/529 (33.84%), Postives = 268/529 (50.66%), Query Frame = 0

Query:  150 FSSHKTRDRTKRKNVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFEDTV---ASDYGNIFRYFKKEGGYKMLGTGKLWH-WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMM----NASVQPD---DNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPR-CCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKE---LYAKMRSIV 662
            FS+  + D  ++ NVLFI  DDLR  L  Y   ++ TP  +RLA+ GVVF++ Y Q  +CNPSR S+++GRR D+ +VW         A D   + ++FK   GY     GK++H W      + ++   P      N G D+         N++  P     + P+E ++D R+A   +  + E    K   PFFL VGF +PH P++ P+KYWDLY+   VP+  +  WP  AP +A              + +  ++D   ++  +A+RE   GY A+IS++D QI R+L+EL+R  L E TIIVF +DHG ++G+  +W K + FE +ARVPLII  P    + G   ++LAE +D++PT+ E+ GLP P+G     +EG SL P+L D  +  V+D AL+Q PR    +G  +                             MGY++R+   RYT W  +                ELYDH+          D  ETRN+A+D   + + KE   L  KM+ IV
Sbjct:   16 FSTPVSAD--EKLNVLFIAADDLRNDLGCYGHALVKTPHLDRLAQRGVVFDKAYCQQAVCNPSRASIMTGRRLDSLRVWDLPTHFRQNAPDIVTLPQHFKN-NGYFTQNIGKIFHNWIHEIQGDPESWSVPAVMHFANHGADKPEVTGELPPNSAQDPKCECRDVPDEAYFDGRVAGLAVKALGEL--KKQEKPFFLAVGFWKPHSPFNAPKKYWDLYKREEVPMPKNPDWPEGAPRIAW------------HNSREILSDKQRQLTPEAVREIRHGYLANISYMDAQIGRVLEELDRQGLTEKTIIVFWSDHGYHLGEQTLWAKTSNFELDARVPLIIATPK-GVTAGQKTASLAELMDLYPTLTELCGLPAPSG-----VEGVSLTPILAD-TSASVRDAALSQHPRPAYYKGAPDA----------------------------MGYTLRSATHRYTEWRDWKTGQTV--------ATELYDHQT---------DPDETRNIAQDQDAEAIVKEHAKLLEKMKPIV 475          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|1133467010|ref|XP_019853892.1| (PREDICTED: iduronate 2-sulfatase-like isoform X1 [Amphimedon queenslandica] >gi|1133467012|ref|XP_019853893.1| PREDICTED: iduronate 2-sulfatase-like isoform X2 [Amphimedon queenslandica] >gi|1133467014|ref|XP_019853894.1| PREDICTED: iduronate 2-sulfatase-like isoform X3 [Amphimedon queenslandica])

HSP 1 Score: 253.832 bits (647), Expect = 1.624e-72
Identity = 184/537 (34.26%), Postives = 274/537 (51.02%), Query Frame = 0

Query:  155 TRDRTKRKNVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFED-----TV--ASDYGNIFRYFKKEGGYKMLGTGKLWH-WTSSFIEEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTK---DGNPFFLGVGFHQPHRPWHMPQKYWDLY---EDRYVPVTPHTTWPIDAPGVA---------TGDINPLEIGMLPDSEK-TYVADPTGRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDP------AGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAKMRS 660
            T   + +KNVLF   DDLRP ++ Y    I +P  + LA   ++FER Y Q  +C+PSR SLL+GRRPDT  VW         TV  A++   I +YFK E GY  +G GK++H    S  ++    + P+    ++   D +     A    D+  P+ +  D +IA   +S++++    +   D  PFF+ VGFH+PH P++   KY+DLY   ED  +P  P    P   P +A           DI  +    L D +    +      VP + ++E  R Y++ +SF D QI R++ EL+     +DTIIV  ADHG  +G+H  W K T FE    VP ++  P + ++ GM   AL E +DIFP++ E+AGL  P      +      +EG S+VP+L +P N + K  + +Q PR  +QG    P K  P F          D +D  E V MGY++RTD++R+T W  FN  T TP + + W G ELYDH  P       F + E  N+A +  ++ V  EL   +++
Sbjct:   16 TSTASGKKNVLFFAVDDLRPEINAYGVDFIKSPNIDALASKSMLFERAYCQIAVCSPSRASLLTGRRPDTNHVWQIAADEYWRTVPDATNATTIPQYFK-ENGYLSIGMGKIFHPGAPSGNDDRMYSWSPEGLPYFHGKEDSSIAKHAAWYSFDE--PDNKRVDGQIADNAVSVLQQIKKNRTEGDDRPFFVAVGFHKPHLPFYCSSKYYDLYPSIEDTKLPENPDA--PTGMPDIAFSIWKELKGYSDIKAIVNETLCDIDADASIHGKECHVPDNKMKELRRAYYSCVSFTDAQIGRVIKELDAQGFADDTIIVLWADHGWQLGEHNEWCKHTNFEDATHVPFLLHVPGVTDN-GMTTKALVELIDIFPSLTELAGLEVPPMCTKNSPTSIACVEGSSVVPLLSNP-NAQWKKGSFSQYPRPSTQGLPEIPGK--PPF----------DPSDKGESV-MGYTVRTDKYRFTEWYRFNRITSTPNFNEIW-GTELYDHSSPTV-----FFNDENVNLATESEMKPVVDELRQLLQA 526          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|910218751|ref|WP_050024602.1| (iduronate sulfatase [Verrucomicrobium sp. BvORR034])

HSP 1 Score: 252.292 bits (643), Expect = 1.657e-72
Identity = 178/535 (33.27%), Postives = 255/535 (47.66%), Query Frame = 0

Query:  150 FSSHKTRDRTKRKNVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFED---TVASDYGNIFRYFKKEGGYKMLGTGKLWH------------WTSSFI----------EEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADPT-GRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAK 657
            F S  +     R NVLFI  DDLRP    Y   ++ +P  +RLA  GV F+  Y Q  +C+PSR+SLL+G RPDTT+V+  +        D   + ++FK EG Y + G GKL+H            WT   +          +E   K   ++  A   G   N      + +  D+ P+  F D  +A   ++ +REA  +    PF+LGVGF +PH P+  P+KYWDLY+   + + P+     DAP  A  +   L         + Y   P  G +P    R+   GY+A+IS++D Q+ RLLDEL+RL L  +TIIV   DHG  +G+HG W K +  E +   PL++  P + +S G  ++AL E VDI+P++ E+ GLP PA      LEG S VPVL +P     K  A +Q PR   +                                 MGYSMRT+++R T WV    +T            ELYDH++         D  E  N+A D S   + K+L A+
Sbjct:    6 FISSISAADKARPNVLFIAVDDLRPEFGAYGSKIVKSPNLDRLAASGVTFQHAYCQQAVCSPSRSSLLTGARPDTTQVYDLQRHFRKFLPDVVTLPQHFKNEG-YFVQGMGKLYHGSLDDPASWSVPWTKPQLPHGVYGLPENQELGKKLTAQAKAA---GKKPNATKRGPAFESSDS-PDNAFHDGALADMAVTALREA--SAKNQPFWLGVGFIRPHLPFVAPKKYWDLYDPAQIELAPNPQPAKDAPPYAVTNFGEL---------RNYAGIPQQGPIPDALARQLKHGYYAAISYMDAQVGRLLDELDRLGLRNNTIIVLWGDHGWKLGEHGSWCKHSTVENDTNAPLLVSVPGL-KSAGQKSAALVEFVDIYPSLAELCGLPLPAH-----LEGTSFVPVLNEPTRAW-KSAAFSQYPRGAKEAG----------------------------VAVMGYSMRTEKYRLTRWVDRTDATKIV-------AVELYDHQN---------DPQENINLAPDPSKAELVKQLSAQ 473          

BLAST of EWM28099.1 vs. NCBI_GenBank
Match: gi|917338815|ref|WP_051945527.1| (iduronate sulfatase [Verrucomicrobium sp. BvORR106])

HSP 1 Score: 252.292 bits (643), Expect = 1.958e-72
Identity = 177/535 (33.08%), Postives = 255/535 (47.66%), Query Frame = 0

Query:  150 FSSHKTRDRTKRKNVLFIMTDDLRPSLSIYNKPVI-TPGFERLAKGGVVFERNYNQDPICNPSRNSLLSGRRPDTTKVWLFEDTVASDYGNIF---RYFKKEGGYKMLGTGKLWH------------WTSSFI----------EEFDNKYFPKSTDAWNDGTDENYQMMNASVQPDDNWPEERFWDYRIASEGISLMREAVNTKDGNPFFLGVGFHQPHRPWHMPQKYWDLYEDRYVPVTPHTTWPIDAPGVATGDINPLEIGMLPDSEKTYVADPT-GRVPSDAIRENVRGYHASISFLDHQIQRLLDELERLSLVEDTIIVFCADHGMNIGDHGMWDKRTLFETNARVPLIIRDPDIPESFGMNASALAENVDIFPTMIEMAGLPDPAGRLFPPLEGKSLVPVLRDPVNGRVKDFALTQIPRCCSQGCGNRPLKKVPWFAWEVCSGGNRDWNDLNERVYMGYSMRTDEWRYTAWVPFNASTHTPEWTQAWGGEELYDHRDPRCNEKGNFDSCETRNMAKDVSLQLVKKELYAK 657
             SS    D+  R NVLFI  DDLRP    Y   ++ +P  +RLA  GV F+  Y Q  +C+PSR+SLL+G RPDTT+V+  +        ++    ++FK EG Y + G GKL+H            WT   +          +E   K   ++  A   G   N      + +  D+ P+  F D  +A   ++ +REA  +    PF+LGVGF +PH P+  P+KYWDLY+   + + P+     DAP  A  +   L         + Y   P  G +P    R+   GY+A+IS++D Q+ RLLDEL+RL L  +TIIV   DHG  +G+HG W K +  E +   PL++  P + +S G  ++AL E VDI+P++ E+ GLP PA      LEG S VPVL +P     K  A +Q PR   +                                 MGYSMRT+++R T WV    +             ELYDH++         D  E  N+A D S   + K+L A+
Sbjct:    7 ISSTHAADKA-RPNVLFIAVDDLRPEFGAYGSKIVKSPNLDRLAASGVTFQHAYCQQAVCSPSRSSLLTGARPDTTQVYDLQRHFRKSLPDVVTLPQHFKNEG-YFVQGMGKLYHGELDDPGSWSVPWTKPKLPHGVYGLPENQELGKKLTAQAKAA---GKKPNAARRGPAFESSDS-PDNAFHDGALADMAVTALREA--SAKNQPFWLGVGFIRPHLPFVAPKKYWDLYDPAQIELAPNPQPAKDAPPFAVTNFGEL---------RNYAGIPQQGPIPDALARQLKHGYYAAISYMDAQVGRLLDELDRLGLRNNTIIVLWGDHGWKLGEHGSWCKHSTVENDTNAPLLVSVPGL-KSAGQKSAALVEFVDIYPSLAELCGLPLPAH-----LEGTSFVPVLNEPTRAG-KSAAFSQYPRGAKEAG----------------------------VAVMGYSMRTEKYRLTRWVDRADAAKVV-------AVELYDHQN---------DPQENLNLATDPSKAELVKQLSAQ 473