EWM28417.1, cds2260 (CDS) Nannochloropsis gaditana

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|585110802|gb|EWM28417.1| (multidrug resistance-associated protein 4 [Nannochloropsis gaditana])

HSP 1 Score: 3715.62 bits (9634), Expect = 0.000e+0
Identity = 1810/1810 (100.00%), Postives = 1810/1810 (100.00%), Query Frame = 0

Query:    1 MASTASGGLKATTHEGSKEGCDEEASTPSTLPSGNRSFFRRFDRADSKQESFGGKEKEEAALEVALTAAEKEVSPKAKVERTRPPSYLEHASWWNILTFGWMNPVIKEGNKRLLTDMDMPALARRDQARHLKELVQSHWESEMKLHPDKPQLWLAMIKAFFPIQLPMYLPFAIVTTCKIVQAHVGIKGLIHVLDRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAVEMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSRPTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFFPDANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVESPTSVAVVKKSKGPRNLPPFLPTRTMSTFSANDGDTSDRDSPGVVEANDGPVLLSTKPTSLTGRIFQGILSLRTPHRSGSDQSDSSRGRSSSRDTAVEMGSKKRGASIQSQALLSDTSVTRTRRTTSALKVVMPNLEFSQDGGMQDILSDEDSDASESGKDEFNFHHQHARPSSPSNLRTSGDSRDRLLSQVLSIKEEEEESSEDEEEDNDGERLLGPVIASTAAESALAVTAGDQEGNKESESVDGEGNGKDGTEEEAHNVEEEQSKREAAERDSIIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMARRQPLAFSFSVGYCECAALSSLWAAYWVFPVRCKGLSDFVHNTLLEVIYMGSRSTCTRTCFDKKLTDRPLSYSLNITRPEPKPDLAYIPPPRKC 1810
            MASTASGGLKATTHEGSKEGCDEEASTPSTLPSGNRSFFRRFDRADSKQESFGGKEKEEAALEVALTAAEKEVSPKAKVERTRPPSYLEHASWWNILTFGWMNPVIKEGNKRLLTDMDMPALARRDQARHLKELVQSHWESEMKLHPDKPQLWLAMIKAFFPIQLPMYLPFAIVTTCKIVQAHVGIKGLIHVLDRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAVEMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSRPTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFFPDANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVESPTSVAVVKKSKGPRNLPPFLPTRTMSTFSANDGDTSDRDSPGVVEANDGPVLLSTKPTSLTGRIFQGILSLRTPHRSGSDQSDSSRGRSSSRDTAVEMGSKKRGASIQSQALLSDTSVTRTRRTTSALKVVMPNLEFSQDGGMQDILSDEDSDASESGKDEFNFHHQHARPSSPSNLRTSGDSRDRLLSQVLSIKEEEEESSEDEEEDNDGERLLGPVIASTAAESALAVTAGDQEGNKESESVDGEGNGKDGTEEEAHNVEEEQSKREAAERDSIIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMARRQPLAFSFSVGYCECAALSSLWAAYWVFPVRCKGLSDFVHNTLLEVIYMGSRSTCTRTCFDKKLTDRPLSYSLNITRPEPKPDLAYIPPPRKC
Sbjct:    1 MASTASGGLKATTHEGSKEGCDEEASTPSTLPSGNRSFFRRFDRADSKQESFGGKEKEEAALEVALTAAEKEVSPKAKVERTRPPSYLEHASWWNILTFGWMNPVIKEGNKRLLTDMDMPALARRDQARHLKELVQSHWESEMKLHPDKPQLWLAMIKAFFPIQLPMYLPFAIVTTCKIVQAHVGIKGLIHVLDRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAVEMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSRPTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFFPDANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVESPTSVAVVKKSKGPRNLPPFLPTRTMSTFSANDGDTSDRDSPGVVEANDGPVLLSTKPTSLTGRIFQGILSLRTPHRSGSDQSDSSRGRSSSRDTAVEMGSKKRGASIQSQALLSDTSVTRTRRTTSALKVVMPNLEFSQDGGMQDILSDEDSDASESGKDEFNFHHQHARPSSPSNLRTSGDSRDRLLSQVLSIKEEEEESSEDEEEDNDGERLLGPVIASTAAESALAVTAGDQEGNKESESVDGEGNGKDGTEEEAHNVEEEQSKREAAERDSIIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMARRQPLAFSFSVGYCECAALSSLWAAYWVFPVRCKGLSDFVHNTLLEVIYMGSRSTCTRTCFDKKLTDRPLSYSLNITRPEPKPDLAYIPPPRKC 1810          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|585110793|gb|EWM28408.1| (multidrug resistance-associated protein 4 [Nannochloropsis gaditana])

HSP 1 Score: 1604.73 bits (4154), Expect = 0.000e+0
Identity = 879/1375 (63.93%), Postives = 1025/1375 (74.55%), Query Frame = 0

Query:  400 MRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSRPTLRHISFDAPVGKILGLAGPVGSGKSTLLLAL-----------------------------------LGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFFPDANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVE---SPTSVAVVKKSKGPRNLPPFLPTRTMSTFSANDGDTSDRDSPGVVEANDGPVLLSTKPTSLTGRIFQGILSLRTPHRSGSDQSDSSRGRSSSRDTAVEMGSKKRGASIQSQAL-LSDTSVTRTRRTTSALKVVMPNLEFSQDGGMQDILSDEDSDASESGKDEFNFHHQHARPSSPSNLRTSGDSRDRLLS-----QVLSIKEE----------EEESSEDEEEDNDGERLLGPVIASTAAESALAVTAGDQEGNKESESVDGEGNGKDGTEEEAHNVEEEQSKREAAERDSIIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFE--TPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMARR 1718
            MRVLIAAGL+RGIHEGI FFSPVLVGAVTFLT W LG QL AG+VFS LTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLE S  P S+S  +PSPP+ TS PPP  VHVNGLSFNWSG  SKSR TLR++S  AP GKILGLAGPVG G  T+++ L                                   LGELG ++  ++++G G +AYASQEPWILTGSVRENILMGRPY+  KY+AVL+AACL+ DL QW+HADYTVIGDRG+NMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELL +GFFPDA +++  +      +   +      + + E   +E   SPT +A+ K++     LPPFLPTRTMS      GD SD +S    E  D  +L    PT L  R  + +  +R    S S+      G S S      +   +  +S Q   L  S ++++  R  +S L V +P+++  Q  GMQ IL++ED D  ++ +DEFN         +P     SG S+  +LS     QV  +++E          EEE   DE+E     ++ G   ++T     + +T  +  G      VD E   K         V+EE+ K+EAA R SII++E Q++G LGAG   AY++GMGG  V +AVL L+ AGQVAAILSN+FLSYWV   +EKQR+  F  IYV LV +VGVL VAR M++FDRML AS  LHDDM+H+VLRAPISFFDTNPTGRIINRT+KDVL+CDV LP++++SF++TML+CLGTI V++SVNPWL+I+L+PL  +FIPLRRQFLNTSR ARRLEA ARSP+YA  SE MDGLPTIRSLVAG+LFQ+EFS+RLD+F RPYFIFFSAGRHL FRLD LCWL++TAT FSSL    A+ G SGVS WL+G+SLMYVLQLA++FQWATRQSS+VENQIVSVERI+EYCQL  EAALDS PG+AP S WPE GAIVATNLQA+YRPGLPLV+K MSFSIPGGTRVGIVGRTGCGKS+LIQ LFRLMEPSGGSLCIDGID S +GLHDLRRRLSVIPQNPFL SGTLREN+DPLG+NDDA IWSALRAVQMEDQ++ MG +   + S+ +  +PR K    P   R R ++ +    A+A     G   +   GPGLDM+VTENGGNFS+GERQLLCLARAILSSTRILVCDEATANVDVETD KIQRAIRSRFGSATVLM+AHRLNTIIDSDILLVLQAGEVLEMGHPHELLNT KGLPESKGGFLSMVMETGPDSAASLKEMA++
Sbjct:    1 MRVLIAAGLLRGIHEGISFFSPVLVGAVTFLTYWSLGRQLKAGQVFSALTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEVSKPPSSDSSLSPSPPLSTSLPPPTPVHVNGLSFNWSGSDSKSRSTLRNVSLAAPPGKILGLAGPVGCGTGTIVIRLSCCRFCFPLACLSCARYLTLTNFATKGKSTLLLALLGELGHFESDIIDVGHGSIAYASQEPWILTGSVRENILMGRPYEKEKYIAVLKAACLETDLTQWEHADYTVIGDRGLNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLVRGFFPDAMVEMNAT------ESTVKLTEMATEAQPEASDIEATLSPT-LALKKQASHLHKLPPFLPTRTMSNIQ-ESGDNSDTESLDT-EEEDVEMLPPKIPT-LVKRFSESLKRIRPSPHSISENPPRLHGPSKS------LSVLRAKSSTQLPVLGRSPSNLSLAREASSYLNVSIPDMDLFQPAGMQRILTEEDFDVEDAEEDEFN---------NPRPQLLSGKSQSTMLSVLERAQVARLQQELSISRERESSEEEDGTDEKEIPVDAKVHG---SATTYAHGVEITELEGNGGARLLQVDKENPKKQEIAANISCVDEEKVKKEAALRASIISQEKQQSGNLGAGVLAAYVRGMGGPIVTVAVLLLLIAGQVAAILSNMFLSYWVHQSYEKQREKKFALIYVALVVAVGVLPVARGMIAFDRMLEASHVLHDDMVHSVLRAPISFFDTNPTGRIINRTSKDVLVCDVFLPISLYSFMKTMLMCLGTIAVIISVNPWLIIILIPLFGSFIPLRRQFLNTSRAARRLEAMARSPIYALLSEVMDGLPTIRSLVAGSLFQNEFSKRLDDFGRPYFIFFSAGRHLAFRLDFLCWLLVTATAFSSLG---ALTGASGVSTWLIGVSLMYVLQLASVFQWATRQSSEVENQIVSVERIVEYCQLSTEAALDSLPGKAPPSAWPEKGAIVATNLQAAYRPGLPLVIKDMSFSIPGGTRVGIVGRTGCGKSSLIQVLFRLMEPSGGSLCIDGIDVSGMGLHDLRRRLSVIPQNPFLLSGTLRENLDPLGLNDDATIWSALRAVQMEDQVRAMGGECEEVESNLDILSPRAK----PPLFRRRSSAMSFMPPAKA-GIRAGSIAVISCGPGLDMYVTENGGNFSIGERQLLCLARAILSSTRILVCDEATANVDVETDHKIQRAIRSRFGSATVLMVAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTSKGLPESKGGFLSMVMETGPDSAASLKEMAQQ 1339          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|553185110|ref|XP_005854201.1| (ATP-binding cassette, subfamily C (CFTR/MRP), member 4 [Nannochloropsis gaditana CCMP526] >gi|422294855|gb|EKU22155.1| ATP-binding cassette, subfamily C (CFTR/MRP), member 4 [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1104.74 bits (2856), Expect = 0.000e+0
Identity = 604/1004 (60.16%), Postives = 726/1004 (72.31%), Query Frame = 0

Query:  735 MQELLAQGFFPDANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVE---SPTSVAVVKKSKGPRNLPPFLPTRTMSTFSANDGDTSDRDSPGVVEANDGPVLLSTKPTSLTGRIFQGILSLRTPHRSGSDQSDSSRGRSSSRDTAVEMGSKKRGASIQSQAL-LSDTSVTRTRRTTSALKVVMPNLEFSQDGGMQDILSDEDSDASESGKDEFNFHHQHARPSSPSNLRTSGDSRDRLLS-----QVLSIKEE----------EEESSEDEEEDNDGERLLGPVIASTAAESALAVTAGDQEGNKESESVDGEGNGKDGTEEEAHNVEEEQSKREAAERDSIIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFE--TPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMAR 1717
            MQELL +GFFPDA +++  +      +   +      + + E   +E   SPT +A+ K++     LPPFLPTRTMS    + GD SD +S    E  D  +L    PT L  R  + +  +R    S S+      G S S      +   +  +S Q   L  S ++++  R  +S L V +P+++  Q  GMQ IL++ED D  ++ +DEFN         +P     SG S+  +LS     QV  +++E          EEE   DE+E     ++ G   ++T     + +T  +  G      VD E   K         V+EE+ K+EAA R SII++E Q++G LGAG   AY++GMGG  V +AVL L+ AGQVAAILSN+FLSYWV   +EKQR+  F  IYV LV +VGVL VAR M++FDRML AS  LHDDM+H+VLRAPISFFDTNPTGRIINRT+KDVL+CDV LP++++SF++TML+CLGTI V++SVNPWL+I+L+PL  +FIPLRRQFLNTSR ARRLEA ARSP+YA  SE MDGLPTIRSLVAG+LFQ+EFS+RLD+F RPYFIFFSAGRHL FRLD LCWL++TAT FSSL    A+ G SGVS WL+G+SLMYVLQLA++FQWATRQSS+VENQIVSVERI+EYCQL  EAALDS PG+AP S WPE GAIVATNLQA+YRPGLPLV+K MSFSIPGGTRVGIVGRTGCGKS+LIQ LFRLMEPSGGSLCIDGID S +GLHDLRRRLSVIPQNPFL SGTLREN+DPLG+NDDA IWSALRAVQMEDQ++ MG +   + S+ +  +PR K    P   R R ++ +    A+A     G   +   GPGLDM+VTENGGNFS+GERQLLCLARAILSSTRILVCDEATANVDVETD KIQRAIRSRFGSATVLM+AHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMAR
Sbjct:    1 MQELLVRGFFPDAMVEMNAT------ESTVKLTEMATEAQPEASDIEATLSPT-LALKKQASHLHKLPPFLPTRTMSNIQES-GDNSDTESLDT-EEEDVEMLPPKIPT-LVKRFSESLKRIRPSPHSISENPPRLHGPSKS------LSVLRAKSSTQLPVLGRSPSNLSLAREASSYLNVSIPDMDLFQPAGMQRILTEEDFDVEDAEEDEFN---------NPRPQLLSGKSQSTMLSVLERAQVARLQQELSISRERESSEEEDGTDEKEIPVDAKVHG---SATTYAHGVEITELEGNGGARLLQVDKENPKKQEIAANISCVDEEKVKKEAALRASIISQEKQQSGNLGAGVLAAYVRGMGGPIVTVAVLLLLIAGQVAAILSNMFLSYWVHQSYEKQREKKFALIYVALVVAVGVLPVARGMIAFDRMLEASHVLHDDMVHSVLRAPISFFDTNPTGRIINRTSKDVLVCDVFLPISLYSFMKTMLMCLGTIAVIISVNPWLIIILIPLFGSFIPLRRQFLNTSRAARRLEAMARSPIYALLSEVMDGLPTIRSLVAGSLFQNEFSKRLDDFGRPYFIFFSAGRHLAFRLDFLCWLLVTATAFSSLG---ALTGASGVSTWLIGVSLMYVLQLASVFQWATRQSSEVENQIVSVERIVEYCQLSTEAALDSLPGKAPPSAWPEKGAIVATNLQAAYRPGLPLVIKDMSFSIPGGTRVGIVGRTGCGKSSLIQVLFRLMEPSGGSLCIDGIDVSGMGLHDLRRRLSVIPQNPFLLSGTLRENLDPLGLNDDATIWSALRAVQMEDQVRAMGGECEEVESNLDILSPRAK----PPLFRRRSSAMSFMPPAKA-GIRAGSIAVISCGPGLDMYVTENGGNFSIGERQLLCLARAILSSTRILVCDEATANVDVETDHKIQRAIRSRFGSATVLMVAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMAR 968          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|553180722|ref|XP_005853451.1| (multidrug resistance-associated protein, partial [Nannochloropsis gaditana CCMP526] >gi|422295608|gb|EKU22907.1| multidrug resistance-associated protein, partial [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 587.415 bits (1513), Expect = 0.000e+0
Identity = 288/390 (73.85%), Postives = 325/390 (83.33%), Query Frame = 0

Query:   82 TRPPSYLEHASWWNILTFGWMNPVIKEGNKRLLTDMDMPALARRDQARHLKELVQSHWESEMKLHPDKPQLWLAMIKAFFPIQLPMYLPFAIVTTCKIVQAHVGIKGLIHVLDRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAVEMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGE 471
            TRPP+YLE AS  +ILTFGWMNPVI EGN+R+LT+ DMP L +RD+A  LK ++QS W++E +LH   PQLW A  KA+FPIQLP+Y+PFAIVT  KIVQAHVGI GLI VL  G   +EG VYA VI   G+I+LML+HQVQFM WRLGL +RTS+E AIFRKILRMPLS+FSS+GA         PG EED DAYKKT+T+AT+LNL TTDVERFQPV+TTSHYL +APFEI AI WCGV +TG PFLIG+ ALL+LIP+Q ++AK+LQ   K ASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRI EARAVEMRVLIAAGL+RGIHEGI FFSPVLVGAVTFLT W LG QL AG+VFS LTLFNILQWDVVNFGGKAMQSLGE
Sbjct:   28 TRPPAYLETASLCSILTFGWMNPVINEGNRRMLTEKDMPELTKRDKAVSLKGVLQSSWDAEKELHSKNPQLWRAAFKAYFPIQLPLYVPFAIVTAAKIVQAHVGITGLIDVLIYGRGFKEGVVYASVIALMGFIVLMLYHQVQFMAWRLGLDMRTSIESAIFRKILRMPLSAFSSEGAVECRA----PG-EEDADAYKKTMTVATILNLVTTDVERFQPVSTTSHYLFMAPFEIAAIAWCGVQQTGFPFLIGIAALLMLIPVQAKIAKLLQQASKHASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIMEARAVEMRVLIAAGLLRGIHEGISFFSPVLVGAVTFLTYWSLGRQLKAGQVFSALTLFNILQWDVVNFGGKAMQSLGE 412          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|299473495|emb|CBN77891.1| (multidrug resistance-associated protein-like protein [Ectocarpus siliculosus])

HSP 1 Score: 516.538 bits (1329), Expect = 5.606e-152
Identity = 288/722 (39.89%), Postives = 417/722 (57.76%), Query Frame = 0

Query: 1020 NKESESVDGEG---NGKDGTEEEAHNVEEEQSKREAAERDS---IIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLME--PSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLS----------------MVMETGPDSAASLKEMAR 1717
             ++ E  DG G   N +D    +A+    E       +R +   ++  E Q++G+L   TY  Y++   GV   + +   +A GQV  +   ++L+ W R   E+Q +   V +  +L  +  V++++RA+++F  +++AS  LH+ M+  V+RAP+ FFD+NP GRI+NR TKD+   D LLP+T++  +    + +G   ++  VNPW+++ L+P    F+ L   +L TSREA+RLEA  RSPVY+  SE +DGL TIR+      F  +F+ R++  +R YF +    R LGFR+D +  +++TA+ F S+A        S V   L+G +L+YVLQL  +FQWA RQ+++VENQ+VSVER++ YC++P EA+L+S PG  P   WP  G I   +L   YR  LP VLKG++ SI GG+RVG+VGRTG GKS+LI ALFRL+E     G + IDG+D S +GLHDLR ++SV+PQ PFLFSG++R N+DP     DA++W++L AVQM+  +Q +                                                    GG  LD  V E GGN S+G+RQLLCLARA+L  ++ILV DEATAN+D  TD  IQ  +R+ F   TV+M+AHRLNT+ID D ++VL  G V+E GHPH LL  P   P S G   +                MV ETG  SA  L+ +A+
Sbjct:  786 QQQQEKKDGWGVGCNREDDFRGDANGDSNEGGLVTKGDRGAPPELVVSEDQESGRLKRSTYWEYIREAAGVWQVVLIFLSMAGGQVLVMGVTVWLARWSRQSEEEQDRSRNVIVLALLSAAAVVVALSRAVLAFFSLVKASHRLHNRMLKRVVRAPVLFFDSNPVGRILNRFTKDMHFMDDLLPMTLYDCITCSFMVMGNTLIIFFVNPWVVLSLLPAMWYFVHLMGFYLKTSREAKRLEAVTRSPVYSQLSETLDGLVTIRAFGKQHRFLGQFTERVNLNTRAYFAWVYTARWLGFRMDMVVIIVLTASCFFSVAVNEY---SSSVDAGLLGAALVYVLQLGGLFQWAVRQAAEVENQMVSVERVLSYCRVPQEASLESEPGYQPSDGWPAKGNIEVRDLSMRYREDLPPVLKGLTLSIKGGSRVGVVGRTGAGKSSLIAALFRLVEYDRDRGGIEIDGVDISKVGLHDLRPKMSVVPQTPFLFSGSMRLNLDPFAKQSDAQMWASLEAVQMKTYVQSLA---------------------------------------------------GG--LDAPVAEGGGNLSVGQRQLLCLARAVLQRSQILVMDEATANIDQHTDSLIQDVVRTSFKGKTVIMVAHRLNTVIDCDQVVVLSEGSVVEAGHPHVLLRDP---PASCGSVAAARETTASAAADATLSSMVDETGVSSAKHLRRLAQ 1448          

HSP 2 Score: 191.43 bits (485), Expect = 4.141e-45
Identity = 113/314 (35.99%), Postives = 172/314 (54.78%), Query Frame = 0

Query:  175 TTCKIVQAHVGIKGLIHVLDRGGPAR-EGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAVEMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPE 487
            + C+++QA + +  LI   D    A  EG+ YA V+V  G ++    H   F+G+ LG+ +R S    ++ KILR+ LS                             I+   ++NL TTDVE FQ   T  +YL  AP + L I++ G+ + G+ FL G  AL L++P+QG  ++      +R + LTD+RV LT QA+ GAR +K++ WE  LEK I+  RA E+RVL+ A L+R I+E ++F  P ++  + F T   LG+ L   +VF+TL L NI Q+ +  F   A+Q+  E WVS+ R+E +L M E
Sbjct:   52 SICRVIQA-LALGWLIGYFDDETSASWEGWTYASVVVLGGLLIGFFLHHFNFVGYMLGMQLRISTTTILYDKILRLRLSLLGQ-------------------------ISTGHLVNLTTTDVEAFQKGGTFVNYLWGAPLQSLVILYFGLDQVGVSFLAGFAALALIVPMQGAFSRRFSQVRQRVTVLTDERVNLTNQAVAGARLMKINAWEPALEKEIRRVRAEEIRVLLKATLLRAINEAMFFVQPAVISCLVFATYHLLGNVLAPRQVFTTLALLNITQFTLGKFLYLAVQTSIESWVSIKRIETILLMEE 339          

HSP 3 Score: 187.963 bits (476), Expect = 4.958e-44
Identity = 100/212 (47.17%), Postives = 139/212 (65.57%), Query Frame = 0

Query:  540 TLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYAS-------QEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFF 744
            TL  I      G+++G+ GPVGS KS+LL+A+L E+    P+  + G G  A          +EPWI +G++RENIL GR  D  +Y AV+R   L+ DL      D T IGD+GVN+SGGQ+AR+GLARMAY  A  YL+DD LSA+DP VGR +FS+++CG LKGSTR+L THQ+ Y   + EV +++ ++ GK+ G+G+ + L A G F
Sbjct:  460 TLDAIDLRIEPGQLVGVIGPVGSSKSSLLMAMLREI---TPERTSGGAGTAAVHGGAGHGHGEEPWIQSGTIRENILFGRAMDQKRYDAVIRDCALERDLSLLPSGDQTGIGDKGVNLSGGQKARVGLARMAYAHADTYLMDDPLSAVDPAVGRELFSKVVCGRLKGSTRVLVTHQVHY-FRDPEVDQIIVMHHGKIVGKGSYEALEASGSF 667          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|299473494|emb|CBN77890.1| (multidrug resistance-associated protein-like protein [Ectocarpus siliculosus])

HSP 1 Score: 514.612 bits (1324), Expect = 1.634e-151
Identity = 290/724 (40.06%), Postives = 416/724 (57.46%), Query Frame = 0

Query: 1016 DQEGNKESESVDGEGNGKDGTEE---EAHNVEEEQSKREAAERDS---IIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLME--PSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGG--------------FLSMVMETGPDSAASLKEMAR 1717
            DQ+ +++ +  DG G G DG  +   E+     E        +D+   ++  E Q++G+L   TY  Y++   GV   + +   +A GQV  +   ++L+ W R   E+Q +   V +  +L  +  V+S+ RA+++F  +++AS  LH+ M+  V+RAP+ FFD+NP GRI+NR TKDV   D LLP+T++  +    + +G   ++  VNPW+++ L+P    F+ L   ++ TSREA+RLEA  RSPVY+  SE +DGL TIR+      F  +F+  ++  +R YF +    R LGFR+D +  +++TA+ F S+A        S V   L+G +++YVLQL  +FQWA RQ+++VENQ+VS ER++ YC++P EAAL S P   P   WP  G I   ++   YR  LP VLKG++ SI GG+RVG+VGRTG GKS+LI ALFRL+E     G + IDG+D S +GLHDLR R+SV+PQ PFLFSG++R N+DP G   DA +W++L AVQM+  +Q +                                                    GG  LD  V E GGN S+G+RQLLCLARA+L  ++ILV DEATAN+D  TD  IQ  +R+ F   TV+M+AHRLNT+ID D ++VL  G V+E GHPH LL  P   P S G                 SMV ETG  SA  L+ +A+
Sbjct:  753 DQQQHQQEK--DGGGVGHDGEADCWSESDGDHNEGGLLTEGAKDAPPELVVSEDQESGRLKRSTYWEYIREAAGVWQVVLIFFSMAGGQVLVMGVTVWLARWSRQSEEEQDRSRNVIVLALLTAAAVVVSLFRAVLTFFSLVKASHRLHNRMLKRVVRAPVLFFDSNPVGRILNRFTKDVHFMDDLLPMTLYDCIMCGFMVMGNTLIIFFVNPWVVLSLLPAMWYFVHLMGFYMKTSREAKRLEAVTRSPVYSQLSETLDGLVTIRAFGKQHRFLGQFTEVVNMNTRAYFAWVYTARWLGFRMDMVVIIVLTASCFFSVAVNEY---SSSVDAGLLGAAMVYVLQLGGLFQWAVRQAAEVENQMVSAERVLSYCRVPQEAALASEPVYKPADGWPAEGNIEVRDMSMRYRDDLPPVLKGLTLSIKGGSRVGVVGRTGAGKSSLIAALFRLVEYDRERGGIEIDGVDISKVGLHDLRPRMSVVPQTPFLFSGSMRLNLDPFGKQSDAHMWASLEAVQMKTYVQSLA---------------------------------------------------GG--LDAPVAEGGGNLSVGQRQLLCLARAVLQRSQILVMDEATANIDQHTDSLIQDVVRTSFKGKTVIMVAHRLNTVIDCDQVVVLSEGSVVEAGHPHVLLRDP---PASCGTTAAACETSAAAYATLSSMVDETGVSSAMHLRRLAQ 1415          

HSP 2 Score: 347.821 bits (891), Expect = 2.917e-94
Identity = 236/693 (34.05%), Postives = 340/693 (49.06%), Query Frame = 0

Query:  194 DRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAVEMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQLDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQA-----PSPPMPTS-------------------SPPPASVHVNGLSFNWSG---------------PASKSRP--------------------------TLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGEL----------------------GEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFFP--------------DANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVESPTS 785
            D    + EG+ YA V+V  G  +    H   F+G+ LG+ +R      ++ KILR+ LSS                            I+   ++NL TTDVE FQ   T  +YL  AP E L I++ G+++ G+ FL G  AL L++P QG  ++      +R + LTD+RV+LT QA+ GAR +K++ WE  LEK I+  RA E+RVL+ A L+R ++E ++F  P ++  + F T   LG+ L   +VF+TL L +I Q+ V  F   A+Q+  E WVS+ R+E +L M    +    AS   P+            P M  S                      P  V + G SF W+                   K R                           TL  I      G+++G+ GPVGS KS+LL+A+L E+                      GE + + +    G LAY SQEPWI + ++RENIL GR  D  +Y AV+R   L+ DL      D T IGDRGVN+SGGQ+AR+GLARMAY  A  YL+DD LSA+DP VGR +FS+++CG LK STR+L THQ+ Y L + EV +++ +++GK+ G+G+ +EL A G F               +AN+D G S         +R+ +G V R     ++ S  S
Sbjct:   24 DEASASWEGWTYASVVVLGGVFICFFIHHFFFVGYTLGMQLRICTTTILYDKILRLRLSSLGQ-------------------------ISTGHLVNLTTTDVEAFQIAGTFVNYLWGAPLESLVILYFGLNQVGVSFLAGFAALALMVPAQGAFSRRFSQVRQRVTVLTDERVKLTNQAVAGARLMKINAWEPALEKEIRRVRAEEIRVLLTATLLRAVNEAMFFIQPAVISCLVFATYHLLGNVLAPRQVFTTLALLSITQFTVGKFLYLAVQTSSESWVSIKRIETILLMEAKTAEAGTASAPTPAAGVAVAGEAREGPSMAGSVRGVREPVGGRDSDGGNGAEEWPGLVSLKGASFRWTSGREEDQHAKVFRGLIEGKKKRKDLGISDGADAAPGEGDNGAETNAGGLTLDAIDLRIEPGQLVGVVGPVGSSKSSLLMAMLREITPERTSGGAGTAAVDGGAGHGHGERNCQPLVSVSGSLAYTSQEPWIQSSTIRENILFGRAMDQKRYDAVIRDCALERDLSLLPSGDQTGIGDRGVNLSGGQKARVGLARMAYAHADTYLMDDPLSAVDPAVGRELFSKVVCGRLKESTRVLVTHQVHY-LRDPEVDQIIVMHQGKIVGKGSYEELEASGSFAALERPNSPTKATRGEANVDHGSST-------VNRNAAGGVSRSTRVRSISSTGS 683          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|871235147|ref|XP_005099201.2| (PREDICTED: multidrug resistance-associated protein 4-like [Aplysia californica])

HSP 1 Score: 484.952 bits (1247), Expect = 4.045e-143
Identity = 279/690 (40.43%), Postives = 385/690 (55.80%), Query Frame = 0

Query: 1061 REGQKAGQLGAGTYGAYMK-GMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKF----HFVA----------------------------IYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAASLKEMAR 1717
            +E + +G +G G Y  Y K G G +     VL  IAA QV  + S+ +LS W  L  ++        HF+A                            I+  ++  V +  + RA++ F   + ASQ+LH+ M   +LR+PISFFD NP GRI+NR +KDV   D LLPVT F F+Q  L+ LG + V   VNP++ I  VPL   F+ +RR +L TSR  +RLE T+RSPV++Y S  + GL T+RS+     F  EF    D  S  +F+F +  R L  RLD LC   + A TF S   V A + M   S  LVG+S+ Y + L  +FQW  RQS++VENQ++SVER++EY +LP EAAL+S P + P  TWP +GAI  T++   Y P  PLVLK  SF I G  +VGIVGRTG GKS+LI  LFR++EP+ G++ IDGI+   +GLHDLR  +++IPQ+P LF+G+LR+N+DP   + D  +W AL  V++ +Q++             E P                                         GL   V+E G NFS+G+RQL+CLARA+L   +IL+ DEATANVD  TD+ IQ  IR++F   TVL IAHRL+TI+DSD ++VL  G ++E   P+ LL       E +G F  M  +TG      L E+A+
Sbjct:  385 QEERASGTVGFGVYLEYFKAGTGVLKFTFLVLVNIAA-QVFYVGSDWWLSRWSNLEEDRYAAIKAHEHFLADNNITDLANTSLSSNITIPYVDSTFNIGIFSAIIAVVFLFGLVRALLFFKIAVDASQTLHNQMFARILRSPISFFDNNPVGRILNRFSKDVGHMDDLLPVTFFDFIQCSLLILGIVLVAGVVNPYVFIPTVPLGIMFVIVRRYYLQTSRGIKRLEGTSRSPVFSYLSATLQGLHTVRSMKMEEKFMQEFDNYQDKHSEAWFLFLATSRWLAVRLDWLCAGFVIAVTFCS---VFAAESM---SPGLVGLSITYTMTLMGMFQWGVRQSAEVENQMISVERVLEYTRLPVEAALESAPDKKPPPTWPSSGAITGTDVNLRYSPDGPLVLKDFSFHINGMEKVGIVGRTGAGKSSLITTLFRMVEPT-GAMVIDGINIQGIGLHDLRNTIAIIPQDPVLFTGSLRKNLDPFSHHKDDVLWKALDEVKLREQVK-------------ELPE----------------------------------------GLGAEVSEGGINFSVGQRQLICLARAVLRHNKILMIDEATANVDPITDELIQATIRTKFRDCTVLTIAHRLHTIMDSDRVMVLDNGRIVEFEEPYTLLK------EGQGYFYEMAQQTGKAEFDHLMEIAK 1007          

HSP 2 Score: 193.356 bits (490), Expect = 7.111e-46
Identity = 107/279 (38.35%), Postives = 160/279 (57.35%), Query Frame = 0

Query:  465 AMQSLGEVWVSLGRLEHLLRMPELP-SYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSRPTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQG 742
            A+ ++ + +V+  R+++ L + EL  S  +E S   P               P  A V ++G++  W G  +  + TL  IS     G+++ + GPVG GKS+LL+++LGEL      V   GR  +AY SQ+PWI +GSVR+NI+ G  +D ++Y  V++ + LK D       D T+IGDRGV++SGGQRAR+ LAR  Y  A +YL+DD LSA+D  VGR +F + I G LK   RIL THQ+Q+L   +    +  +  GK+  QG+  +L   G
Sbjct:   62 AVFTIADTYVAKVRVQNFLLLEELDHSSNVEKSNLRPK--------------PEDAFVQLDGVTAKWEGEGTSEQNTLESISLSVKAGQLVAVIGPVGCGKSSLLMSILGELPTQKGDVRVSGR--VAYVSQQPWIFSGSVRQNIVFGGVFDKSRYDKVVKVSALKRDFEIMPQGDATLIGDRGVSLSGGQRARVSLARALYMDADIYLLDDPLSAVDTAVGRHIFEKCIVGYLKHKPRILVTHQVQFLPVAD---NIFILKEGKIESQGSYDDLSQSG 321          

HSP 3 Score: 65.855 bits (159), Expect = 7.357e-7
Identity = 77/319 (24.14%), Postives = 137/319 (42.95%), Query Frame = 0

Query:  447 TLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSRPTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVN------IG----RGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRA---ACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLICGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKV-----------RGQGTMQELLAQ 741
            T+TL  + QW V        +   EV   +  +E +L    LP   +EA+     +  + P P  P+S     +  VN L ++  GP       L+  SF     + +G+ G  G+GKS+L+  L   +      V++      IG    R  +A   Q+P + TGS+R+N+    P+ H K   + +A     L+  + +        + + G+N S GQR  I LAR       + +ID+A + +DP +   +    I    +  T +   H+L  +++ +   +V+ ++ G++            GQG   E+  Q
Sbjct:  702 TMTLMGMFQWGV--------RQSAEVENQMISVERVLEYTRLP---VEAALESAPD--KKPPPTWPSSGAITGT-DVN-LRYSPDGPL-----VLKDFSFHINGMEKVGIVGRTGAGKSSLITTLFRMVEPTGAMVIDGINIQGIGLHDLRNTIAIIPQDPVLFTGSLRKNL---DPFSHHKDDVLWKALDEVKLREQVKELPEGLGAEVSEGGINFSVGQRQLICLARAVLRHNKILMIDEATANVDP-ITDELIQATIRTKFRDCTVLTIAHRLHTIMDSD---RVMVLDNGRIVEFEEPYTLLKEGQGYFYEMAQQ 993          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|552816363|ref|XP_005844664.1| (hypothetical protein CHLNCDRAFT_138549 [Chlorella variabilis] >gi|307104308|gb|EFN52562.1| hypothetical protein CHLNCDRAFT_138549 [Chlorella variabilis])

HSP 1 Score: 440.654 bits (1132), Expect = 1.945e-125
Identity = 260/661 (39.33%), Postives = 369/661 (55.82%), Query Frame = 0

Query: 1058 IIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAG----QVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVLSVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSLCWLMMTATTFSSLATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIR--SRFGS--ATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGLPESKGGFLSMVMETGPDSAA 1710
            +I  E    G +    YG Y     G  +G+ V+TLI AG    Q   +  + +LS W     E QR+ ++V  Y I+VG++  +S +R+++ F   LRAS  LHD+M+  VLRAP+SFF T+PTGRI+NR +KD    D  LP   F  V  +++ LG   +++   P+++ + +PL A F  +R ++L TSRE +R EAT+RSPV+A FS  + GLPTIR+  AG  F+  F + L +    +F F +  R +GFRLD L  L+MTA     +A          +S  L G++L   LQLA + QW  RQS++VEN + SVER++ Y +L  E A  +  G  P   WP  G I   ++ A YR GLP VL+ ++F++ GG   G+VGRTG GKS+L+  LFRL+  + G++ IDG+DTS + L  LRR++++IPQ+P LFSGTLR N+DP G  +D ++W ALRA Q+   +  +G                                               G+D        H+ E G N S+G+RQL CLARA+L    I+  DEATANVD +TD  IQ A+R  +R G    T+L+IAHR++TI+D D LLVL AG +LE G P +       L +S G F  +V      SAA
Sbjct:  838 LIVAEDHAVGSVPWAVYGRY-----GARMGLPVVTLITAGLLLGQAIYLFGDYWLSLWASKDGEAQREVYWVWGYAIMVGAILAISFSRSLLFFMASLRASTQLHDEMVQRVLRAPLSFFHTSPTGRILNRFSKDQGQVDEQLPQVFFDCVLALMMVLGAFALLMVSVPFIIPLFLPLGAAFFLVRARYLRTSREIKRWEATSRSPVFASFSAILKGLPTIRAFGAGPRFRSAFLQELSDNGAWWFCFLTTARWIGFRLDLLVALLMTAAPLLMMAV------HDRLSARLAGLALTQSLQLAGMLQWMVRQSAEVENNMTSVERMLHYTRLEQEPATLAGGGPKPPPDWPAAGRIEYRDVTAIYRSGLPPVLRDLTFTLEGGVSCGVVGRTGSGKSSLMLTLFRLIPVTSGAILIDGLDTSGVALDALRRQVAIIPQDPVLFSGTLRSNLDPWGTFEDGRLWEALRAAQLGGVVAALG-----------------------------------------------GLD-------AHMQEAGDNLSVGQRQLFCLARALLQDAAIVALDEATANVDRQTDALIQEAVRQCTRRGDRRRTLLVIAHRIDTIMDCDQLLVLSAGRLLEQGAPGQ-------LAQSGGMFARLVAAARGSSAA 1426          

HSP 2 Score: 150.214 bits (378), Expect = 1.685e-32
Identity = 97/218 (44.50%), Postives = 134/218 (61.47%), Query Frame = 0

Query:  541 LRHISFDAPVGKILGLAGPVGSGKSTLLLALLGEL--------------GEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLIC--GLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQG 742
            LR + F    G ++G+ G VGSGKS++L ALLGEL                  P V    RG +AY+SQ PWI++ +V+ENIL G+ Y+  +Y AVLRA  L+ D+      D T +G+RG+N+SGGQ+AR+ LAR AY  A + L+DD LSA+DP+VGR +F   I   GL+ G+TR+L THQ Q+L   +E   VL +  G++  +GT  EL A G
Sbjct:  455 LRGLRFSVARGSLVGIVGTVGSGKSSVLAALLGELQPTQRGGASAAGAGVAAAPPVTV--RGSVAYSSQVPWIVSSTVKENILFGQRYEATQYEAVLRACALEDDITALPAGDETELGERGINLSGGQKARLALARAAYSQAEIQLLDDPLSAVDPRVGRILFDDCIAGTGLMAGTTRLLVTHQRQFLPGCDE---VLVLRGGEIAHRGTHAELAAAG 667          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|1134812280|gb|OMJ19580.1| (Metal resistance protein YCF1 [Smittium culicis] >gi|1134818976|gb|OMJ24151.1| Metal resistance protein YCF1 [Smittium culicis])

HSP 1 Score: 421.009 bits (1081), Expect = 9.531e-118
Identity = 260/750 (34.67%), Postives = 410/750 (54.67%), Query Frame = 0

Query:  963 GDSRDRLLSQVLSIKEEEEESSEDEEEDNDGERLLGPVIASTAA---ESALA-------VTAGDQEGNKESESVDGEG--NGKDGTEEEAHNVEEEQSKREAAERDSIIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRL---PFEKQRKFHFVAIYVILVGSVGVL-----SVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSL-CWLMMTATTFSSL-ATVSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGL 1690
            G S  R+L      K  E +S+E  E++ +    L   + ST A   E++L+       +  G    N +S ++D +      D    E++   +E+   +A +   +I  E  K+G++    +  Y+K  G +S  +  L  +      ++++N++L YW      P + +   +++ IY    G  G+L     S     +     +RA++  H  ++ AV+R+P+SFFDT P GRI+NR +KD    D  +P +  S+  +M+  L T+ V+V+  P  L+  VP+   +  +   ++ +SRE +RLE+T RSP+Y+ F E + G+ TIR+      F+   ++ L   +R  F + S  R +  RL+S+   ++  A+ F+ + A ++  KG S +S   VG+++ Y L +     W  RQ++++EN IVSVER  EYC+LPPEA  +  P   P + WP  G IV +N    YR GL L+L+ ++ +IP G++VG+VGRTG GKS+L  ALFR++E +GG + IDGID ST+GL DLR ++ +IPQ+P LFS TLR N+DP     D +IWSAL   +++               S+   +    GT ++  S  ++                     G  GL++ + + G N SLG+RQL+CLARA+L  ++IL+ DEATA +DVETD+ IQ  IR+ F + TV+ IAHRLNTIIDSD +LVL+ GE  E  +P  LL  P  L
Sbjct:  871 GGSLVRILGDEHFTKINENDSNEVSEQNVES---LNSGLNSTQASLKENSLSRRASISTIGTGFDFSNFDSSTLDSQSMIESPDLIMPESNVGSQEKGASQAGQ---LIEDEVSKSGRIKLRNFVDYVKA-GSISGMLIYLVSLVLTNTFSVVANVWLQYWSNQNVKPGDSRSATYYILIY----GLFGLLQAIFLSTQNFTIWVFIAIRAAKKTHSLLLKAVMRSPMSFFDTTPLGRILNRFSKDQETIDHQIPRSFDSWASSMMSVLATLIVIVTSFPLFLVAAVPIMIAYYFVYDYYIVSSRELKRLESTTRSPIYSMFQECLSGVSTIRAYGHVNRFKRLNNKLLTINTRAQFPYISLNRWISIRLESIGALIIFFASLFAVITALINHNKGNSSISAARVGLAVSYSLSVTQNLSWGIRQTAELENSIVSVERNKEYCELPPEAP-EIIPDNRPPTEWPTRGEIVLSNYSTRYREGLDLILRNINLTIPAGSKVGVVGRTGAGKSSLSLALFRIIESAGGKILIDGIDISTIGLKDLRSKIVIIPQDPVLFSETLRANLDPFNEFSDVEIWSALEKSKLK---------------SWVIQQDTQTGTDATGSSETSAVPTPTTTS-------------GSLGLNVMIQQGGANMSLGQRQLVCLARALLRRSKILILDEATAAIDVETDRFIQDVIRTEFATCTVITIAHRLNTIIDSDYILVLEKGEAAEFDNPQALLRNPDSL 1580          

HSP 2 Score: 244.973 bits (624), Expect = 1.644e-61
Identity = 201/791 (25.41%), Postives = 360/791 (45.51%), Query Frame = 0

Query:   90 HASWWNILTFGWMNPVIKEGNKRLLTDMDMPALARRDQARHLKELVQSHWESEMKLHPDKPQLWLAMIKAF-FPIQLPMYLPFAIVTTCKIVQAHV--GIKGLIHVL----DRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAV-EMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQ----LDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSR----------------------------------PTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLI--CGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQGFFPDANLDVGLSPRHGDDDHRDRSVSGLVKRKEEEETVESPTS-VAVVKKSKGPRNLPPFLPTRTMST-FSANDGDTSDRDSPGVVEAND 830
             A+ ++ + F WM+ ++K G KR+L   D+  L       +  E  +  W+ E+  +P+ P LW  M ++F  P+ + M+L   I       Q  +   + G I +     D      +G++ +  ++F      M   Q   + +  G+  +T++   +F+K   M LS                       ++ ++ ++   + NL + D  +   V +  + +   P + +  +       G+  L G++ +++ +PI   ++K+++D  KR   + D+R+RL  + +   ++IK+  WE     +  E R   E++ L + G + G    +      LV  +TF     L ++    L+A  VF +L+LFN+L++ +      A   + E  VS  R+  LL   +L S  ++    P S                   V V   +F W   A+ S                                   P++++I+F+A  G+++G+ G VGSGKS+L+ ALLG++ +    V  + RG +AY  Q+PWI+  ++RENIL G+ YD   Y  V++   L+ D+   +  D   IG++G+N+SGGQ+AR+ LAR  Y  A +YL+DD LSA+D  VGR + + +I   GLL    RIL TH +QYL     + +V+ ++ G++  +G   EL+ +         VG S      D     ++     +  E+ VES  S +   + S    +L       T+ T F  ++ D+S  DS  ++E+ D
Sbjct:  204 EANIYSRIYFLWMSDLLKTGRKRILAIKDVWPLPDYLDVENTTETFEKEWDVEIARYPNSPNLWRVMYRSFGTPLLISMFLKL-IQDILNFTQTLLFSQLLGFIAIYTSQTDNSISITKGFIISFAMLFNSLFQTMFLQQYFHLAFNTGIKAKTAITNVVFKK--SMNLS-----------------------NSARQKLSTGDIFNLMSVDANKISDVVSYINTIWSGPLQAIVCVILLYKTIGVSALTGVVVMVIGVPINIYMSKMMRDIQKRQMTIKDKRLRLIDEILSSIKSIKLYSWESAFIDKTTEVRNKGELKNLRSYGKIYGYQSFVTSIVAFLVSFLTFAIYSALDNKSRGPLNAQLVFVSLSLFNLLKFPLFILPMIA-SFVIEANVSCKRVIKLLVSEDLQSDAVDKQ--PFSREEGNLKIKAGAIENRDLMVSVTNGTFTWGFNANNSNDSKNDKKKLSKKEKKEMKEKKSQPSDENVYDEDTPSIKNINFNAYYGELIGIVGIVGSGKSSLVSALLGDMKKLSGDV--MLRGRVAYVPQQPWIINSTLRENILFGQKYDKEFYERVIKNCELEKDIAMLEGGDMAEIGEKGINLSGGQKARLSLARAVYSRAHVYLLDDPLSAVDAHVGRNIMTNVIGPSGLLHNKCRILVTHAVQYL---PLIDQVIVMDNGEICEKGHYNELINK---------VGGSLVRILGDEHFTKINENDSNEVSEQNVESLNSGLNSTQASLKENSLSRRASISTIGTGFDFSNFDSSTLDSQSMIESPD 951          

BLAST of EWM28417.1 vs. NCBI_GenBank
Match: gi|1134826229|gb|OMJ29407.1| (Metal resistance protein YCF1 [Smittium culicis])

HSP 1 Score: 410.223 bits (1053), Expect = 3.804e-114
Identity = 233/640 (36.41%), Postives = 361/640 (56.41%), Query Frame = 0

Query: 1058 IIAREGQKAGQLGAGTYGAYMKGMGGVSVGIAVLTLIAAGQVAAILSNLFLSYWVRLPFEKQRKFHFVAIYVILVGSVGVL-----SVARAMVSFDRMLRASQSLHDDMIHAVLRAPISFFDTNPTGRIINRTTKDVLMCDVLLPVTMFSFVQTMLICLGTIGVVVSVNPWLLIVLVPLAATFIPLRRQFLNTSREARRLEATARSPVYAYFSEAMDGLPTIRSLVAGTLFQDEFSRRLDNFSRPYFIFFSAGRHLGFRLDSL-CWLMMTATTFSSLAT-VSAVKGMSGVSEWLVGMSLMYVLQLAAIFQWATRQSSDVENQIVSVERIMEYCQLPPEAALDSTPGRAPRSTWPETGAIVATNLQASYRPGLPLVLKGMSFSIPGGTRVGIVGRTGCGKSTLIQALFRLMEPSGGSLCIDGIDTSTLGLHDLRRRLSVIPQNPFLFSGTLRENMDPLGMNDDAKIWSALRAVQMEDQIQVMGVDFGMIGSSFETPRGKHKGTPSSRRSRVASFAISHDARADSALQGFKGMDLGGPGLDMHVTENGGNFSLGERQLLCLARAILSSTRILVCDEATANVDVETDQKIQRAIRSRFGSATVLMIAHRLNTIIDSDILLVLQAGEVLEMGHPHELLNTPKGL 1690
            +I  E  K+G++    +  Y+K      +GI +++L+       +++N++L YW      K         Y+++ G  G+L     S     +     +RA++  H  ++ AV+R+P+SFFDT P GRI+NR +KD    D  +P +  S+  +M+  L T+ V+V+  P  L   VP+   +  +   ++ +SRE +RLE+T RSP+Y+ F E ++G+ TIR+      F+   ++ L   +R  F + S  R +  RL+S+   ++  A+ F+ + T ++  KG + +S   VG+++ Y L +     W  RQ++++EN IVSVER  EYC+LPPEA  +  P   P + WP  G IV +N    YR GL L+L+ ++ +IP G++VG+VGRTG GKS+L  ALFR++E + G + IDGID S++GL DLR ++ +IPQ+P LFS TLR N+DP     D +IWSAL   +++               S+   +  H G  ++  S  A+   S                     L++ + + G N SLG+RQL+CLARA+L  ++IL+ DEATA +DVETD+ IQ  IR+ F + TV+ IAHRLNTIIDSD +LVL+ GE  E   P  LL  P  L
Sbjct:  972 LIESEVSKSGRIKMKNFVDYVKAGSLSGMGIYLVSLVLTNTFG-VVANVWLQYWSNQNI-KTTDSRSSTYYILIYGLFGLLQAIFLSTQNFTIWVFIAIRAAKKTHSLLLKAVMRSPMSFFDTTPLGRILNRFSKDQETIDHQIPRSFDSWASSMMSVLATLIVIVTSFPLFLAAAVPIMVAYYFVYDYYIVSSRELKRLESTTRSPIYSMFQECLNGVSTIRAYGHVNRFKKLNTKLLTINTRAQFPYISLNRWISIRLESIGALIIFFASLFAVITTLINYNKGNTSISAARVGLAVSYSLSVTQNLSWGIRQTAELENSIVSVERNKEYCELPPEAP-EIIPDNRPPTEWPTRGEIVLSNYSTRYREGLDLILRNINLTIPAGSKVGVVGRTGAGKSSLSLALFRIIESAEGKILIDGIDISSIGLKDLRSKIVIIPQDPVLFSETLRANLDPFDEFSDVEIWSALEKSKLK---------------SWVIQQDTHTGAETTGSSETAAVTTSTTTSGSLG-------------LNVMIQQGGANMSLGQRQLVCLARALLRRSKILILDEATAAIDVETDRFIQDVIRTEFANCTVITIAHRLNTIIDSDYILVLEKGEAAEFDDPQALLRNPDSL 1580          

HSP 2 Score: 239.58 bits (610), Expect = 7.492e-60
Identity = 179/700 (25.57%), Postives = 321/700 (45.86%), Query Frame = 0

Query:   90 HASWWNILTFGWMNPVIKEGNKRLLTDMDMPALARRDQARHLKELVQSHWESEMKLHPDKPQLWLAMIKAF-FPIQLPMYLPFAIVTTCKIVQAHV--GIKGLIHVL----DRGGPAREGYVYAGVIVFTGWIMLMLHHQVQFMGWRLGLAIRTSLECAIFRKILRMPLSSFSSQGAEGKEGDEVVPGKEEDVDAYKKTITMATVLNLATTDVERFQPVATTSHYLVLAPFEILAIMWCGVHETGLPFLIGMLALLLLIPIQGRVAKVLQDYGKRASQLTDQRVRLTKQAIQGARTIKMSGWEVLLEKRIKEARAV-EMRVLIAAGLVRGIHEGIYFFSPVLVGAVTFLTDWGLGSQ----LDAGRVFSTLTLFNILQWDVVNFGGKAMQSLGEVWVSLGRLEHLLRMPELPSYRLEASTTPPSNSFQAPSPPMPTSSPPPASVHVNGLSFNWSGPASKSR----------------------------------PTLRHISFDAPVGKILGLAGPVGSGKSTLLLALLGELGEYDPKVVNIGRGCLAYASQEPWILTGSVRENILMGRPYDHAKYLAVLRAACLKPDLLQWDHADYTVIGDRGVNMSGGQRARIGLARMAYGAASLYLIDDALSALDPKVGRRVFSRLI--CGLLKGSTRILATHQLQYLLEEEEVPKVLFINRGKVRGQGTMQELLAQ 741
             A+ ++ + F WM+ ++K G +R+L   D+  L       +  E  +  W+ E+  +P  P LW  M ++F  P+ + M+L   I       Q  +   + G I +     D      +G++ +  ++F      M   Q   + +  G+  +T++   +F+K L +  S+                         ++ ++   + NL + D  +   V +  + +   P + +  +       G+  L G++ +++ +PI   ++K ++D  KR   + D+R+RL  + +   ++IK+  WE     +  E R   E++ L + G + G    +      LV  +TF       ++    L+A  VF +L+LFN+L++ +      A   + E  VS  R+  LL   +L S  +E    P S                   V V   +F W   A+ S                                   P++++I+F+A  G+++G+ G VGSGKS+L+ ALLG++ +    V  + RG +AY  Q+PWI+  + RENIL G+ YD   Y  V++   L+ D+   +  D   IG++G+N+SGGQ++R+ LAR  Y  A +YL+DD LSA+D  VGR + + +I   GLL    RIL TH +QYL     + +V+ ++ G+V  +G   EL+ +
Sbjct:  204 EANVYSRIYFLWMSDLLKTGRQRVLAIKDVWPLPDYLDVDYTTETFEKEWDVEIAKYPKSPNLWRVMYRSFGTPLLISMFLKL-IQDILNFTQTMLFSQLLGFIAIYTSQTDNSISITKGFIISFAMLFNSLFQTMFLQQYFHLSFNTGIKAKTAITNVVFKKSLNLSNSA-------------------------RQKLSTGDIFNLMSVDANKISDVVSYINTIWSGPLQAVVCVILLYKTIGVSALTGVVVMVIGVPINIYMSKKMRDIQKRQMTIKDKRLRLIDEILSSIKSIKLYSWESAFIDKTTEVRNKGELKNLRSYGKIYGYQSFVTSIVAFLVSFLTFAIYSAFDNKSRGPLNAQLVFVSLSLFNLLKFPLFILPMIA-SFVIEANVSCKRVIKLLVSEDLQSDAVERQ--PFSREEGNLKIKAGDIKNRDLMVSVTNGTFTWGFNANNSNNSTQDNKKLSKKEKKEKKEKKSQPSDENLYDEDSPSIKNINFNAYYGELIGIVGIVGSGKSSLVSALLGDMKKLSGDV--MLRGRVAYVPQQPWIINSTFRENILFGQKYDKEFYDRVIKNCELEKDIAMLEGGDMAEIGEKGINLSGGQKSRLSLARAVYSRAHVYLLDDPLSAVDAHVGRNIMTNVIGPSGLLHNKCRILVTHAVQYL---PLIDQVIVMDNGEVCEKGHYNELINK 869