EWM28421.1, cds2247 (CDS) Nannochloropsis gaditana

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|585110808|gb|EWM28421.1| (mdn1 midasin family protein [Nannochloropsis gaditana])

HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0
Identity = 318/318 (100.00%), Postives = 318/318 (100.00%), Query Frame = 0

Query:    1 MNPANDAGKKELPPALRARFTEVEVTELVDPQDLRAVVERAWSARGGGGGKGGCGVDPIDLVQLYLRCRQLAESVLVDGPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRSMRGP 318
            MNPANDAGKKELPPALRARFTEVEVTELVDPQDLRAVVERAWSARGGGGGKGGCGVDPIDLVQLYLRCRQLAESVLVDGPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRSMRGP
Sbjct:    1 MNPANDAGKKELPPALRARFTEVEVTELVDPQDLRAVVERAWSARGGGGGKGGCGVDPIDLVQLYLRCRQLAESVLVDGPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRSMRGP 318          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|224000641|ref|XP_002289993.1| (predicted protein, partial [Thalassiosira pseudonana CCMP1335] >gi|220975201|gb|EED93530.1| predicted protein, partial [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 291.967 bits (746), Expect = 4.049e-91
Identity = 150/235 (63.83%), Postives = 176/235 (74.89%), Query Frame = 0

Query:   80 PFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRS 314
            PFW+ +G     DW  +  S   G  +F+LTPSA   LR L +AVA G  + ILL+GPTSAGKTTLVEYLAAR GH CVRINNHEHTD+ EYTG+YA D   G + FQ+G+LV+ALR+G W+ILDELNLAP EVLE LNRLLDDNREL +PE  E + PH  FRLFATQN P GAYGGRKPLSRAFRNRF+E+ M  I  +EM  IL  R G  PSHA+LLVKVM +L+ RR+R+
Sbjct:    6 PFWIVTGNEEQLDW-ADPSSSFDGRPKFVLTPSATANLRRLCQAVASGPWS-ILLEGPTSAGKTTLVEYLAARVGHRCVRINNHEHTDIQEYTGSYAADS-KGKISFQEGILVQALRKGHWVILDELNLAPSEVLEALNRLLDDNRELYLPEINETVKPHKNFRLFATQN-PAGAYGGRKPLSRAFRNRFVEIHMADIPEDEMILILERRCGCPPSHAKLLVKVMKSLRQRRSRT 236          

HSP 2 Score: 65.855 bits (159), Expect = 2.587e-8
Identity = 41/96 (42.71%), Postives = 64/96 (66.67%), Query Frame = 0

Query:  188 DGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPET-----QEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIA 278
            DG LV+++++G+ L+LDE++LA   VLE LN +L+ +R L++ E       E+I  +  FR+FAT N PGG +G R+ LS A R+RF E+ + P+ 
Sbjct:  493 DGPLVRSMKDGSMLLLDEMSLAEDAVLERLNSVLEPSRTLVLAEKGGEGPSEVIA-NDKFRIFATMN-PGGDFGKRE-LSPALRSRFTEIWVPPVT 585          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|397646202|gb|EJK77168.1| (hypothetical protein THAOC_01019 [Thalassiosira oceanica])

HSP 1 Score: 290.426 bits (742), Expect = 2.763e-85
Identity = 148/236 (62.71%), Postives = 176/236 (74.58%), Query Frame = 0

Query:   80 PFWLPSGPLPLRDWNQEEGSGGP-GTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRS 314
            PFW+ SG     DW   + S  P G  +F+LTPSA   LR L +AVA G  + +LL+GPTSAGKTTLVEYLA R GH CVRINNHEHTD+ EYTG+YA D   G + FQ+G+LV+ALR+G W+ILDELNLAP EVLE LNRLLDDNREL + E  E++ PH  FRLFATQN P GAYGGRKPLSRAFRNRF+E+ +  I  +EM  IL +R    PSHA+LLVKVM TL+ RR+R+
Sbjct: 1270 PFWIKSGEEKQIDWT--DASLSPDGKPKFVLTPSASGNLRRLCQAVASGPWS-VLLEGPTSAGKTTLVEYLARRCGHKCVRINNHEHTDIQEYTGSYAADS-KGKISFQEGILVQALRKGYWVILDELNLAPSEVLEALNRLLDDNRELYLAEINEVVKPHENFRLFATQN-PAGAYGGRKPLSRAFRNRFVEIHLSDIPEDEMVTILEKRCACPPSHAKLLVKVMKTLRQRRSRT 1500          

HSP 2 Score: 121.324 bits (303), Expect = 9.484e-27
Identity = 82/215 (38.14%), Postives = 117/215 (54.42%), Query Frame = 0

Query:  102 PGTKRFILT-PSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGA---YAPDP---ITG-HLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQ 308
            P    F LT P+  L LR + R +      PILL+GP   GKT+L+  LA  +GH  VRIN  E TD+S+  G+   Y+ D     +G   ++ DG+L+KA++ G W++LDELNLA   VLEGLN  LD    + +PE        P FR+FA QNP     GGRK L ++F NRF ++ +  +   ++  I+ ++    PS    LV+ M    
Sbjct: 2074 PTNLNFDLTAPTTGLNLRRVLRGMQ--ISKPILLEGPPGVGKTSLIAALAKASGHRLVRINLSEQTDISDLMGSDLPYSGDSEGSTSGPSFKWCDGVLLKAIKRGDWVLLDELNLASQSVLEGLNSCLDHRASVYIPELGRSFDCPPTFRVFAAQNPLAQG-GGRKGLPKSFLNRFTKVYIEALTDSDLFSIVLKQF---PSIPENLVRSMICFN 2282          

HSP 3 Score: 72.7886 bits (177), Expect = 1.789e-10
Identity = 41/90 (45.56%), Postives = 56/90 (62.22%), Query Frame = 0

Query:  186 FQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNR-ELLVPET--QEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            F +G LV A+R G W++LDE+NLA  E L+ L  LLDD++  +++ E    E +  HP FRLFA  NP   A  G+K L  + R+RF EL
Sbjct: 1034 FTEGALVDAIRTGKWVLLDEINLASSETLQRLCGLLDDSQGSIILTEKGDAEAVSRHPDFRLFAAMNPATDA--GKKDLPSSIRSRFTEL 1121          

HSP 4 Score: 65.4698 bits (158), Expect = 4.610e-8
Identity = 38/85 (44.71%), Postives = 50/85 (58.82%), Query Frame = 0

Query:    1 MNPANDAGKKELPPALRARFTEVEVTELVDPQDLRAVVERAWSARGGGGGKGGCGV------DPIDLVQLYLRCRQLAESVLVDG 79
            MNPA DAGKK+LP ++R+RFTE+ V EL+D   LR+V     +AR   G     GV        +  V LYL+CR L++  L DG
Sbjct: 1099 MNPATDAGKKDLPSSIRSRFTELYVDELLDSTQLRSV-----AARYLEGAVVTSGVPLEHTESVLSSVDLYLKCRSLSDQSLADG 1178          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|298706627|emb|CBJ29565.1| (midasin-like protein [Ectocarpus siliculosus])

HSP 1 Score: 285.804 bits (730), Expect = 1.016e-83
Identity = 150/236 (63.56%), Postives = 175/236 (74.15%), Query Frame = 0

Query:   79 GPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRS 314
            GPFWL  GP    D  + + +     +RF+LT S    LR LARAVA    +P+LLQGPTSAGKT++VEYLAAR GH CVRINNHEHTD+ EYTG+YA D   G L FQ+G+LV++LR+G W+ILDELNLAP EVLE LNRLLD NREL + ETQE I PHP FRLFATQNPP GAYGGRKPLSRAFRNRF+E+ +  I   E E IL ER G+ PSH +LLVKV+  LQ +R  S
Sbjct:  306 GPFWLEGGPHDPVDAAELDPT--TNQRRFVLTASVGGTLRCLARAVASSD-SPVLLQGPTSAGKTSIVEYLAARLGHRCVRINNHEHTDIQEYTGSYAADG-NGKLAFQEGVLVESLRKGYWIILDELNLAPSEVLEALNRLLDHNRELYLSETQETIKPHPNFRLFATQNPP-GAYGGRKPLSRAFRNRFMEVHVDDIPGAEWEIILAERCGLPPSHCKLLVKVLHELQAQRQSS 536          

HSP 2 Score: 122.094 bits (305), Expect = 4.929e-27
Identity = 83/223 (37.22%), Postives = 114/223 (51.12%), Query Frame = 0

Query:   80 PFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPIT---------------GHLRFQ--DGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQI 285
            PF+ P GP  +       GS        +  P+    LR + RA+       +LL+G    GKT+L+  LAA TGH  VRIN  E TDVS+  G+  P P T                + RF   DG+ + AL+ G W++LDELNLA   VLEGLN  LD   E+ +PE        P F++FA QNP G   GGRK L R+F +RF ++ + P+  +++  I
Sbjct: 1236 PFFTPCGPAKVL------GSESANLDFSVEAPTTSKNLRRVLRALQIRKA--VLLEGSPGVGKTSLISALAAATGHELVRINLSEQTDVSDLFGSDLPVPDTDSGEQTGQPTEGSAGNNARFSWCDGVFLSALKAGRWVLLDELNLATQSVLEGLNACLDHRAEVYIPELGRSFKCPPTFKVFAAQNPLGQG-GGRKGLPRSFLSRFTKVYVDPLTPDDLLYI 1449          

HSP 3 Score: 68.9366 bits (167), Expect = 4.061e-9
Identity = 43/111 (38.74%), Postives = 62/111 (55.86%), Query Frame = 0

Query:  165 HTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNR-ELLVPETQEI--IVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            H++   +    A    +    F +G+LVKA+REG W++LDE+NLA  E L+ L+ LL+     L + E  ++  +V H  FRLFA  NPP  A   +K L  A R RF E+
Sbjct:   55 HSNAERFERQRAAVEGSFAFSFVEGVLVKAIREGKWVLLDEINLASAETLQRLSGLLEGAEGSLCLTERGDMNPVVRHKDFRLFAAMNPPTDA--SKKDLPPALRRRFSEV 163          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|914569470|gb|KOB73106.1| (Midasin, partial [Operophtera brumata])

HSP 1 Score: 283.493 bits (724), Expect = 6.995e-83
Identity = 153/315 (48.57%), Postives = 202/315 (64.13%), Query Frame = 0

Query:    1 MNPANDAGKKELPPALRARFTEVEVTELVDPQDLRAVVERAWSARGGGGGKGGCGVDPIDLVQLYLRCRQLAESVLVDGP-FWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRTRS 314
            MNPA D GKK+LP  LR RFTE  + EL +  DL  ++          G      ++ I     Y   ++ A+  LVDG   W+P G L +   N  EG        +ILT + R  LR +AR +  G   P+LLQG TS GKT+L+ Y+A  +G+ CVRINNHEHTD+ EY G+YA D  +G L F++G+LV+A+R+G W+ILDELNLAP +VLE LNR+LDDNREL +PETQ+++   P F LFATQNPP G YGGRK LSRAFRNRF+EL    I   E+E ILH+R  + P++++ ++ VM+ LQ RR  S
Sbjct:  700 MNPATDVGKKDLPVGLRNRFTEFFIDELTERNDLMLLIGDYLYHMNLSGAT----LEAI--YSFYATIKKEAKLTLVDGAGHWIPQGKLEI---NIPEG--------YILTQTVRKNLRDIARIICLGR-LPVLLQGDTSVGKTSLITYIAKASGNYCVRINNHEHTDLQEYIGSYATDS-SGKLVFKEGVLVEAMRKGYWIILDELNLAPSDVLEALNRVLDDNRELFIPETQQVVRADPNFMLFATQNPP-GLYGGRKMLSRAFRNRFVELHFDEIPKNELETILHQRCHVPPAYSKKMINVMSDLQIRRRGS 994          

HSP 2 Score: 114.005 bits (284), Expect = 2.673e-24
Identity = 70/180 (38.89%), Postives = 97/180 (53.89%), Query Frame = 0

Query:  117 LRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNP--PGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAP 294
            LRGL    A      ILL+G    GKT+LV  LA  +GH  VRIN  + TD+++  G   P    G   F++G  + AL+ G W++LDELNLAP  VLEGLN  LD   E+ +PE        P  +LFA QNP   GGA   R+ L  +F NRF ++ +  +  +++  I+  +    P
Sbjct: 1468 LRGLQLNKA------ILLEGNPGVGKTSLVTALAKSSGHKVVRINLSDQTDITDLFGTDLPTE-DGSFTFKEGAFLNALQNGDWILLDELNLAPQPVLEGLNACLDHRGEVFIPELGRAFKVKPQTKLFACQNPLKQGGA---RRGLPLSFLNRFTQVYIDTLTKQDLIYIVESQYPSIP 1637          

HSP 3 Score: 87.0409 bits (214), Expect = 4.155e-15
Identity = 58/153 (37.91%), Postives = 80/153 (52.29%), Query Frame = 0

Query:  131 PILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAP---DPITGHL-RFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQE-------IIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            P+LL G T  GKTT+ + LAA      + +N H HT+ S++ G   P       G L  + DG L+KA+  G   + DE++LA   VLE LN LL+  R+L++ E          +I     F    T N PGG + G+K LS A RNRF E+
Sbjct: 1125 PVLLVGETGCGKTTICQVLAALKQRQLMSVNCHMHTESSDFLGGLRPVRQYKNDGRLFEWVDGPLIKAMETGDMFLADEISLADDSVLERLNSLLEPERQLVLAERGMEDNSDIVVITAETNFHFIGTMN-PGGDF-GKKELSPALRNRFTEI 1275          

HSP 4 Score: 76.6406 bits (187), Expect = 1.118e-11
Identity = 42/93 (45.16%), Postives = 56/93 (60.22%), Query Frame = 0

Query:  182 GHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEI--IVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            G   + D LLVK L EG+WL+LD +NL    VL+ LN LL+ N  L +PE  ++  I PHP FR+F T +P  G       +SRA RNR +E+
Sbjct: 1948 GKFEWVDSLLVKTLVEGSWLLLDNVNLTSAAVLDRLNGLLEPNGVLSIPERGDVSDIKPHPNFRVFFTMDPKYGE------ISRAMRNRGVEI 2034          

HSP 5 Score: 70.0922 bits (170), Expect = 1.641e-9
Identity = 41/90 (45.56%), Postives = 52/90 (57.78%), Query Frame = 0

Query:  186 FQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRE---LLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            F +G LVKA+++G W++LDE+NLA  E+LE L  LL+D  E   LL    +  I  HP F LFA  NP      G+K L    RNRF E 
Sbjct:  635 FIEGSLVKAMQKGHWVLLDEINLASAEILECLAGLLEDKNESIDLLEKGDKVPIQRHPDFTLFACMNPATDV--GKKDLPVGLRNRFTEF 722          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|302845014|ref|XP_002954046.1| (hypothetical protein VOLCADRAFT_121252 [Volvox carteri f. nagariensis] >gi|300260545|gb|EFJ44763.1| hypothetical protein VOLCADRAFT_121252, partial [Volvox carteri f. nagariensis])

HSP 1 Score: 282.337 bits (721), Expect = 1.994e-82
Identity = 180/350 (51.43%), Postives = 213/350 (60.86%), Query Frame = 0

Query:    1 MNPANDAGKKELPPALRARFTEVEVTELVDPQDLRAVVERAWSARGGGGGKGGCGVDPID-LVQLYL--RCRQLAESV---------------------LVDGP-------------FWLPSGPLPL-RDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRRT 312
            MNPA DAGK ELP ALR RFTE+ V E    +DL AV+  A+ A       G     P+D +V  YL  +   LA S                      L+  P             FWL  GPL L R   +++GSG    +RF++TPS R  L  LARA A     PILLQGPTSAGKT+LV YLAA+TGH  VRINNHEHTD+ EY G+Y  D   G L F++G LV+A+R G W++LDELNLAP EVLE LNRLLDDNRELLVPE  E++ PHP F LFATQNPPGGAY GRK LSRAFR+RFLEL +  I   E+ +IL +   +APS+A  LV V   LQ RR 
Sbjct: 1058 MNPATDAGKHELPAALRNRFTEMWVPEPAAREDL-AVLVAAYLA-------GVGAAPPVDAVVDFYLAAKAEALAPSAAPRMEALIAKHLLPGVKNAKALLRAPPEPAGGTHVLFEAFWLERGPLELPRGGREDDGSG----RRFVMTPSVRQHLTNLARA-ALIRKHPILLQGPTSAGKTSLVAYLAAQTGHTFVRINNHEHTDLQEYLGSYVSD-AAGRLVFREGPLVQAVRRGWWVVLDELNLAPTEVLEALNRLLDDNRELLVPELGEVVRPHPHFLLFATQNPPGGAYAGRKVLSRAFRSRFLELHIDDIPDSELAEILEKCCAIAPSYAAKLVAVQRELQRRRN 1393          

HSP 2 Score: 87.0409 bits (214), Expect = 3.567e-15
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 0

Query:  103 GTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGH--VCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFAT 247
            G   F+ T + R  L   A A+    G P+LL+GP  AGKT +VE LAARTG+    VR++  +  D     GAY    + G   +Q G L +A+ EG W++++++N+AP +VL  L  LL ++R L VP    ++   P F++ AT
Sbjct:  322 GGHCFVATETVRRNLE--AAALVLCQGLPLLLEGPPGAGKTRMVEELAARTGNGSSMVRVHLDDQMDAKSLLGAYVCTAVPGEFAWQPGPLTQAVSEGRWVLVEDINMAPGDVLAALVPLL-ESRVLHVPSRGLVVPASPGFQIIAT 465          

HSP 3 Score: 69.3218 bits (168), Expect = 3.081e-9
Identity = 58/164 (35.37%), Postives = 76/164 (46.34%), Query Frame = 0

Query:  132 ILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDP--ITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQI---LHERV 290
            +LL+G    GKT+LV  +A             + TD+ +  GA  P P    G   + DG L+ ALR G W++LDELNLA   VLEGLN LLD   E                        P G  GGRK L R+F NRF  + +  +  +++  I   LH RV
Sbjct: 1998 VLLEGSPGVGKTSLVAAMA-------------KATDMMDLLGADLPAPGGAPGQFAWCDGPLLGALRAGHWVLLDELNLAGQSVLEGLNALLDHRSE-----------------------NPLGEGGGRKGLPRSFLNRFTRVSVELLRRDDLLFIAGALHPRV 2125          

HSP 4 Score: 65.855 bits (159), Expect = 3.373e-8
Identity = 49/121 (40.50%), Postives = 60/121 (49.59%), Query Frame = 0

Query:  186 FQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDD-------------------NRELLVPETQEIIVP-HPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQIL 286
            F +G+LVKALR G WL+LDE+NLAP EVLE L  LL+                        L+     + VP HP FRL A  NP   A  G+  L  A RNRF E+ +   AA E   +L
Sbjct:  976 FVEGILVKALRNGWWLLLDEINLAPAEVLERLAGLLETAGGDSSAAQADAAGGGGSGGSITLLERGDTVAVPRHPNFRLVAAMNPATDA--GKHELPAALRNRFTEMWVPEPAAREDLAVL 1094          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|343426303|emb|CBQ69834.1| (related to MDN1-Huge dynein-related AAA-type ATPase (midasin) [Sporisorium reilianum SRZ2])

HSP 1 Score: 280.026 bits (715), Expect = 1.010e-81
Identity = 142/233 (60.94%), Postives = 167/233 (71.67%), Query Frame = 0

Query:   79 GPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRR 311
            GPFWLP+GP PL              + +ILTPS +  L GL+RA A     P+L+QGPTSAGKT+ VEYLA RTGH  VRINNHEHTD+ EY G+YA DP +G L FQ+GLLVKALR+G W++LDELNLAP +VLE LNRLLDDNREL++PET E++ PHP F LFATQNPP G Y GRK LSRAFRNRFLEL    +   E+E IL  R  +APS+A  +V V   LQ RR
Sbjct: 1179 GPFWLPTGPAPL-----------DAAEDYILTPSVQSKLVGLSRA-ALTRRFPVLIQGPTSAGKTSAVEYLARRTGHRFVRINNHEHTDIQEYIGSYASDPDSGKLSFQEGLLVKALRKGDWIVLDELNLAPTDVLEALNRLLDDNRELVIPETGEVVKPHPHFMLFATQNPP-GLYAGRKVLSRAFRNRFLELHFDDVPRAELETILTNRCKIAPSYASRIVGVFEELQKRR 1398          

HSP 2 Score: 113.235 bits (282), Expect = 5.269e-24
Identity = 77/208 (37.02%), Postives = 112/208 (53.85%), Query Frame = 0

Query:   79 GPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAP----DPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEM 282
            GPF +  G L L        S G G+  F    SA  A+R L RA++   G  +LL+G   AGKT+L+  LAA  G    RIN  + T++ +  G+  P     P  G   ++D   + A+++G W++LDE+NLA   VLEGLN  LD    + VPE       HP FR+FA QNP     GGRK L ++F NRF+++ +  +  +++
Sbjct: 1892 GPFAISRGSLDL-------PSQGTGSFSFGAKTSASNAMRVL-RALSVP-GRSVLLEGSPGAGKTSLITALAAAAGRPLTRINLSDQTELVDLFGSDMPLEGGGP--GEFAWRDAAFLTAMQQGEWVLLDEMNLASQTVLEGLNSCLDHRGTVYVPELGRSFTKHPNFRIFAAQNPHHQG-GGRKGLPKSFLNRFIKVHIEELTGDDI 2087          

HSP 3 Score: 68.1662 bits (165), Expect = 6.161e-9
Identity = 42/116 (36.21%), Postives = 64/116 (55.17%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGP---IAAEEMEQILH 287
            TG   + DG L++AL+EG WL+LD  NL    VL+ LN L + N  L++ E         ++ PHP FR+F   +P  G       LSRA RNR +E+ + P    A+ ++E+++ 
Sbjct: 2395 TGKFEWIDGPLLRALQEGHWLMLDNANLCSASVLDRLNSLFEVNGSLVISERGVVDGKVPVVRPHPNFRVFMCLDPRHG------ELSRAMRNRGIEIVLLPQQGTASSDLERLVQ 2504          

HSP 4 Score: 66.2402 bits (160), Expect = 2.849e-8
Identity = 40/92 (43.48%), Postives = 53/92 (57.61%), Query Frame = 0

Query:  184 LRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDD-NRELLVPETQEI--IVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
              F +G LVKALR+G W++LDE+NLA  E L+ L  LL      + + E  ++  I  HP FRLFA  NP      G+K L  + R+RF EL
Sbjct:  947 FSFVEGPLVKALRQGDWILLDEINLAAAETLDSLTGLLQSPTSSITLTERGDLEPIPRHPNFRLFACMNPATDV--GKKDLPASLRSRFTEL 1036          

HSP 5 Score: 62.3882 bits (150), Expect = 5.157e-7
Identity = 42/106 (39.62%), Postives = 58/106 (54.72%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE--------------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            T    +QDG LV+A+R G  ++LDE++LA   VLE LN +L+  R L++ E              +   I     F++ AT N PGG Y G+K LS A RNRF E+
Sbjct: 1646 TALFEWQDGPLVQAMRAGDHILLDEISLADDSVLERLNSVLEPARTLVLAERATSSSQLNSEADLSASQITGADGFQVLATMN-PGGDY-GKKELSPALRNRFTEI 1749          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|913889942|gb|KNZ64278.1| (hypothetical protein VP01_1047g1, partial [Puccinia sorghi])

HSP 1 Score: 275.018 bits (702), Expect = 3.188e-81
Identity = 140/239 (58.58%), Postives = 172/239 (71.97%), Query Frame = 0

Query:   73 ESVLVDGPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRR 311
            +S +  G FWL           Q      P T+ ++LT S +  +  LARAV  G   P+L+QGPTS+GKT++V Y+A  TGH  VRINNHEHTD+ EY G+YA DP TG L F++G LV+ALR+GAW++LDELNLAP +VLE LNRLLDDNRELL+PETQEI+ PHP F LFATQNPP G YGGRK LSRAFRNRFLEL  G +  +E+E IL +R  +APSHA+ +V+V A LQ RR
Sbjct:  326 DSYIQVGSFWLKKA--------QVLDESVP-TQEYVLTESVKSKIVDLARAVTTGRW-PVLIQGPTSSGKTSIVAYIARLTGHPFVRINNHEHTDIQEYIGSYATDPETGRLCFKEGALVRALRQGAWVVLDELNLAPSDVLEALNRLLDDNRELLIPETQEIVKPHPDFMLFATQNPP-GLYGGRKVLSRAFRNRFLELHFGDVPTDELEVILCQRCAIAPSHAKKIVQVFAELQRRR 553          

HSP 2 Score: 71.2478 bits (173), Expect = 5.207e-10
Identity = 41/98 (41.84%), Postives = 62/98 (63.27%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            T    + DG L++A++EG +++LDE++LA   VLE LN LL+  R +++ E       +  I  H +F++FAT N PGG +G R+ LS A RNRF E+
Sbjct:  773 TALFDWSDGPLIQAMQEGNYVLLDEISLADDSVLERLNSLLEPERSIVLAERGGESLDKMQITAHKSFQIFATMN-PGGDFGKRE-LSPALRNRFTEI 868          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|443900021|dbj|GAC77348.1| (translation initiation factor 2C [Moesziomyces antarcticus T-34])

HSP 1 Score: 278.1 bits (710), Expect = 6.126e-81
Identity = 141/233 (60.52%), Postives = 166/233 (71.24%), Query Frame = 0

Query:   79 GPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRR 311
            GPFWLP+GP PL              + +ILTPS +  L GL+RA A     P+L+QGPTSAGKT+ VEYLA RTGH  VRINNHEHTD+ EY G+YA DP +G L FQ+GLLVKALR G W++LDELNLAP +VLE LNRLLDDNREL++PET E++ PHP F LFATQNPP G Y GRK LSRAFRNRFLEL    +   E+E IL  R  +APS++  +V V   LQ RR
Sbjct: 1205 GPFWLPTGPAPL-----------DAAEDYILTPSVQSKLVGLSRA-ALVRRFPVLIQGPTSAGKTSAVEYLARRTGHRFVRINNHEHTDIQEYIGSYASDPDSGKLSFQEGLLVKALRRGDWIVLDELNLAPTDVLEALNRLLDDNRELVIPETGEVVKPHPHFMLFATQNPP-GLYAGRKVLSRAFRNRFLELHFDDVPRAELETILTNRCKIAPSYSSRIVGVFEELQKRR 1424          

HSP 2 Score: 102.449 bits (254), Expect = 2.779e-20
Identity = 75/209 (35.89%), Postives = 111/209 (53.11%), Query Frame = 0

Query:   79 GPFWLPSG-PLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAP----DPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEM 282
            GPF +P G P P         + G G+  F    SA  A+R L RA++      +LL+G   AGKT+L+  LAA       RIN  + T++ +  G+  P     P  G   ++D   + A+++G W++LDE+NLA   VLEGLN  LD    + VPE       HP FR+FA QNP     GGRK L ++F NRF+++ +  +  +++
Sbjct: 1918 GPFSVPRGQPDP--------STQGSGSFSFGAKTSASNAMRVL-RALSVP-AKSVLLEGSPGAGKTSLITALAAAAARPLTRINLSDQTELVDLFGSDMPLEGGGP--GEFAWRDAAFLTAMQQGEWVLLDEMNLASQTVLEGLNSCLDHRGTVYVPELGRSFAKHPDFRIFAAQNPHHQG-GGRKGLPKSFLNRFIKVHIEELDGDDI 2113          

HSP 3 Score: 67.3958 bits (163), Expect = 1.356e-8
Identity = 41/102 (40.20%), Postives = 56/102 (54.90%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGP 276
            TG   + DG L++AL+EG WL+LD  NL    VL+ LN L + N  L++ E         +I PHP FR+F   +P  G       LSRA RNR +E+ + P
Sbjct: 2421 TGRFEWIDGPLLRALQEGHWLMLDNANLCSASVLDRLNSLFEPNGCLVISERGVVDGKVPVIRPHPNFRVFMCLDPRHG------ELSRAMRNRGIEIALLP 2516          

HSP 4 Score: 66.6254 bits (161), Expect = 2.443e-8
Identity = 40/92 (43.48%), Postives = 53/92 (57.61%), Query Frame = 0

Query:  184 LRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDD-NRELLVPETQEI--IVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
              F +G LVKALR+G WL+LDE+NLA  E L+ L  LL      + + E  ++  I  HP FR+FA  NP      G+K L  + R+RF EL
Sbjct:  973 FSFVEGPLVKALRQGDWLLLDEINLAAAETLDSLTGLLQSPTSSITLTERGDLEPIPRHPNFRIFACMNPATDV--GKKDLPASLRSRFTEL 1062          

HSP 5 Score: 62.3882 bits (150), Expect = 5.797e-7
Identity = 42/106 (39.62%), Postives = 57/106 (53.77%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE--------------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            T    +QDG LV+A+R G  ++LDE++LA   VLE LN +L+  R L++ E                  I     F++ AT N PGG Y G+K LS A RNRF E+
Sbjct: 1672 TALFEWQDGPLVQAMRAGDHILLDEISLADDSVLERLNSVLEPARTLVLAERATSSSHLGSEADLMSSQITGAEGFQVLATMN-PGGDY-GKKELSPALRNRFTEI 1775          

BLAST of EWM28421.1 vs. NCBI_GenBank
Match: gi|955486254|ref|XP_014655203.1| (huge dynein-related AAA-type ATPase Mdn1p [Moesziomyces antarcticus] >gi|674217934|dbj|GAK66788.1| huge dynein-related AAA-type ATPase Mdn1p [Moesziomyces antarcticus])

HSP 1 Score: 277.715 bits (709), Expect = 6.428e-81
Identity = 141/233 (60.52%), Postives = 166/233 (71.24%), Query Frame = 0

Query:   79 GPFWLPSGPLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAPDPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEMEQILHERVGMAPSHARLLVKVMATLQGRR 311
            GPFWLP+GP PL              + +ILTPS +  L GL+RA A     P+L+QGPTSAGKT+ VEYLA RTGH  VRINNHEHTD+ EY G+YA DP +G L FQ+GLLVKALR G W++LDELNLAP +VLE LNRLLDDNREL++PET E++ PHP F LFATQNPP G Y GRK LSRAFRNRFLEL    +   E+E IL  R  +APS++  +V V   LQ RR
Sbjct: 1203 GPFWLPTGPAPL-----------DAAEDYILTPSVQSKLVGLSRA-ALVRRFPVLIQGPTSAGKTSAVEYLARRTGHRFVRINNHEHTDIQEYIGSYASDPDSGKLSFQEGLLVKALRRGDWIVLDELNLAPTDVLEALNRLLDDNRELVIPETGEVVKPHPHFMLFATQNPP-GLYAGRKVLSRAFRNRFLELHFDDVPRAELETILTNRCKIAPSYSSRIVGVFEELQKRR 1422          

HSP 2 Score: 102.064 bits (253), Expect = 3.384e-20
Identity = 75/209 (35.89%), Postives = 111/209 (53.11%), Query Frame = 0

Query:   79 GPFWLPSG-PLPLRDWNQEEGSGGPGTKRFILTPSARLALRGLARAVAGGHGAPILLQGPTSAGKTTLVEYLAARTGHVCVRINNHEHTDVSEYTGAYAP----DPITGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPETQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGPIAAEEM 282
            GPF +P G P P         + G G+  F    SA  A+R L RA++      +LL+G   AGKT+L+  LAA       RIN  + T++ +  G+  P     P  G   ++D   + A+++G W++LDE+NLA   VLEGLN  LD    + VPE       HP FR+FA QNP     GGRK L ++F NRF+++ +  +  +++
Sbjct: 1916 GPFSVPRGQPDP--------STQGSGSFSFGAKTSASNAMRVL-RALSVP-AKSVLLEGSPGAGKTSLITALAAAAALPLTRINLSDQTELVDLFGSDMPLEGGGP--GEFAWRDAAFLTAMQQGEWVLLDEMNLASQTVLEGLNSCLDHRGTVYVPELGRSFAKHPDFRIFAAQNPHHQG-GGRKGLPKSFLNRFIKVHIEELDGDDI 2111          

HSP 3 Score: 67.0106 bits (162), Expect = 1.419e-8
Identity = 41/102 (40.20%), Postives = 56/102 (54.90%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLELDMGP 276
            TG   + DG L++AL+EG WL+LD  NL    VL+ LN L + N  L++ E         +I PHP FR+F   +P  G       LSRA RNR +E+ + P
Sbjct: 2419 TGRFEWIDGPLLRALQEGHWLMLDNANLCSASVLDRLNSLFEPNGCLVISERGVVDGKVPVIRPHPNFRVFMCLDPRHG------ELSRAMRNRGIEIALLP 2514          

HSP 4 Score: 66.6254 bits (161), Expect = 2.421e-8
Identity = 40/92 (43.48%), Postives = 53/92 (57.61%), Query Frame = 0

Query:  184 LRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDD-NRELLVPETQEI--IVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
              F +G LVKALR+G WL+LDE+NLA  E L+ L  LL      + + E  ++  I  HP FR+FA  NP      G+K L  + R+RF EL
Sbjct:  971 FSFVEGPLVKALRQGDWLLLDEINLAAAETLDSLTGLLQSPTSSITLTERGDLEPIPRHPNFRIFACMNPATDV--GKKDLPASLRSRFTEL 1060          

HSP 5 Score: 62.3882 bits (150), Expect = 5.694e-7
Identity = 42/106 (39.62%), Postives = 57/106 (53.77%), Query Frame = 0

Query:  181 TGHLRFQDGLLVKALREGAWLILDELNLAPPEVLEGLNRLLDDNRELLVPE--------------TQEIIVPHPAFRLFATQNPPGGAYGGRKPLSRAFRNRFLEL 272
            T    +QDG LV+A+R G  ++LDE++LA   VLE LN +L+  R L++ E                  I     F++ AT N PGG Y G+K LS A RNRF E+
Sbjct: 1670 TALFEWQDGPLVQAMRAGDHILLDEISLADDSVLERLNSVLEPARTLVLAERATSSSHLGSEADLMSSQITGAEGFQVLATMN-PGGDY-GKKELSPALRNRFTEI 1773