EWM28520.1, cds2154 (CDS) Nannochloropsis gaditana

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|585110937|gb|EWM28520.1| (ubiquitin protein ligase e3a [Nannochloropsis gaditana])

HSP 1 Score: 2945.99 bits (7636), Expect = 0.000e+0
Identity = 1434/1434 (100.00%), Postives = 1434/1434 (100.00%), Query Frame = 0

Query:    1 MRRSLSETSIAVLFRLERLQVEVDSIAQQVTEIENAIEARRANVAPASVLEGLDALTCNLKGIEAQTHAHSHDHIESGHEEAGLLARRIQSKGRAVSERIARMKKELPLAGMEVADGVSAPVEDEEGSMLELPDVVPCNGHMDDQTALPPPFASVGGLDPGSVHRSKSCGLPARPGEKGTDMDRETGVEGATNIHADEARNGQDHLEDERSAWHSETELYSWGRTDLGSSFQCLGPFGSEGGGGLFQMEVEEGKREEAGTAAGHGPNMLASTRQYSRSLSLPTAPLFAPVQSWYSRKNVVNLCSTLYHTVAVTLTGEVYGAGVNEDGQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEVVQEPQQVLETAGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQSFAVGLGTGVATPSRRSELPVLSRDVDARELVRKFSVIKPAASCVGVGYLLSLIHGAARASDMDTLERTLIDVFSSPSLLNGSFLTLPTSSSRADKEELDGNGCIFPDSHVGNKIIDDIIRAPVGGAERAAGEKSNARSSSCGTLSASASGLAVQGLEAVYVRAMKLNSPPLLSRLVQAMSGALDNLVSDAKNLADPDSLRCLLAYWQCPLNSNTSLSKDTFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLRINFGKGRAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGLQ 1434
            MRRSLSETSIAVLFRLERLQVEVDSIAQQVTEIENAIEARRANVAPASVLEGLDALTCNLKGIEAQTHAHSHDHIESGHEEAGLLARRIQSKGRAVSERIARMKKELPLAGMEVADGVSAPVEDEEGSMLELPDVVPCNGHMDDQTALPPPFASVGGLDPGSVHRSKSCGLPARPGEKGTDMDRETGVEGATNIHADEARNGQDHLEDERSAWHSETELYSWGRTDLGSSFQCLGPFGSEGGGGLFQMEVEEGKREEAGTAAGHGPNMLASTRQYSRSLSLPTAPLFAPVQSWYSRKNVVNLCSTLYHTVAVTLTGEVYGAGVNEDGQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEVVQEPQQVLETAGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQSFAVGLGTGVATPSRRSELPVLSRDVDARELVRKFSVIKPAASCVGVGYLLSLIHGAARASDMDTLERTLIDVFSSPSLLNGSFLTLPTSSSRADKEELDGNGCIFPDSHVGNKIIDDIIRAPVGGAERAAGEKSNARSSSCGTLSASASGLAVQGLEAVYVRAMKLNSPPLLSRLVQAMSGALDNLVSDAKNLADPDSLRCLLAYWQCPLNSNTSLSKDTFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLRINFGKGRAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGLQ
Sbjct:    1 MRRSLSETSIAVLFRLERLQVEVDSIAQQVTEIENAIEARRANVAPASVLEGLDALTCNLKGIEAQTHAHSHDHIESGHEEAGLLARRIQSKGRAVSERIARMKKELPLAGMEVADGVSAPVEDEEGSMLELPDVVPCNGHMDDQTALPPPFASVGGLDPGSVHRSKSCGLPARPGEKGTDMDRETGVEGATNIHADEARNGQDHLEDERSAWHSETELYSWGRTDLGSSFQCLGPFGSEGGGGLFQMEVEEGKREEAGTAAGHGPNMLASTRQYSRSLSLPTAPLFAPVQSWYSRKNVVNLCSTLYHTVAVTLTGEVYGAGVNEDGQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEVVQEPQQVLETAGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQSFAVGLGTGVATPSRRSELPVLSRDVDARELVRKFSVIKPAASCVGVGYLLSLIHGAARASDMDTLERTLIDVFSSPSLLNGSFLTLPTSSSRADKEELDGNGCIFPDSHVGNKIIDDIIRAPVGGAERAAGEKSNARSSSCGTLSASASGLAVQGLEAVYVRAMKLNSPPLLSRLVQAMSGALDNLVSDAKNLADPDSLRCLLAYWQCPLNSNTSLSKDTFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLRINFGKGRAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGLQ 1434          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|545704607|ref|XP_005704626.1| (ubiquitin-protein ligase E3 [Galdieria sulphuraria] >gi|452821071|gb|EME28106.1| ubiquitin-protein ligase E3 [Galdieria sulphuraria])

HSP 1 Score: 488.034 bits (1255), Expect = 2.254e-147
Identity = 362/1159 (31.23%), Postives = 555/1159 (47.89%), Query Frame = 0

Query:  327 GQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECG--GHADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAG--------AGVEV---VQEPQQVLETAGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQS---FAVGLGTGVATPSRRSELPVLSRDVDARELVRKFSVIKPAASCVGV--GYLLSLIHGA-ARASDMDTLERTLIDVFSSPSLLNGSFL---TLPTSSSRADKEELDGNGCIFPDSHVGNKIIDDIIRAPVGGAERAAGEK-SNARSSSCGTLSASASGLAVQG--LEAVYVRAMKL---NSPPLLSRLVQAMSGALDNLVS-------DAKNLADPDSLRCLLAYWQCPLNSNTSLSKDTFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLR-INFGKGRAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQM---------------FQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGLQ 1434
             Q + +RQEP      +  P   +R   ++CG++     T SG +V++G    GQLG  A    S   GP  V  L ++ V +VA G    L LT  G+V+SCG+G  G  G  +  + ++   V+AL   PV  +A G +H++ IT TG  +A+G N++GQ G             E+   +  PQ+ L +    +AACG  H++ L  +G+V ACG    G LG+                          G+ +D           C  V   +  L    +  +A G  H+  L+  GE++  GSN  GQLG   P+   S+ +           +D   +F  +   E+S   F + + TG         + ++ ++ +           K A+S   +   YL++L     +   D+  L++ L    S+ S+ + SFL      T SS         N  IFP  +    I         G       E  S  + S+  T   S   L +Q   +E+ Y   + +   N+  LL+ + + +    +N+++       +  N +   ++    +  +  ++S     K    +LC  + SL L  +  ++  V + +P  L A RL+RPLH+ I+  L  +     RA+    V +   + ASE   W                  +++   +F N +VS L D  L  D   W    E  +  +++F+FC + ++L   AK R+L+ EA    ++A + +++R +               F  G   PY  L V R  L+   L+ +   D   L+K L+V FL EEGVDEGG++KEFFQL+TA+LF   YGMF   E  R  WF        D   ++LVGI++G+ALYN V+LDLHFPL+VYKKLL     L D+ E  PE+   L  +LDYEED            +E VF   F + +  F E++  EL PGG  I V  +N+ +++  Y++++   SI   F+AF  G+  ++ G ++ + RP+EL +LVCG  HL+   L+ V  YEGG+ AE  VIK FW +V EM L  + + L FVTGS +AP+GGLG+L  +IQRAGPD+D LPT+HTCFN LLLPEY SKEK + RL+ AI   +GFGLQ
Sbjct:   83 NQTATNRQEP----IRIQSPYTRERTQSIACGEDFVILATQSGTLVTWGGGAYGQLGRGAPMDWS---GPHPVKNLQNKRVVKVAAGAFHWLALTDAGQVYSCGLGRHGQTGLGNCEDVYEPCLVEALWAQPVVNIAAGENHSIVITSTGFAFAFGSNKYGQLGISRQRLPESETRAELPLRIYMPQE-LASERFLAAACGTHHTLFLTSNGKVLACGSGQDGVLGI--------------------------GNNKD---------EVCLVV---IFSLFEYTIVQIAAGARHSAALSSQGELFLWGSNEQGQLGFVEPTCVFSLPTQ----------MDTNIIFKDSFEKEESSSYFFLKIATGA-----YHTIGLIGKEKE-----------KCASSPFEIYPSYLMNLTSMQWSSVRDLLPLQKRL----SAASIFSHSFLYGTVTGTDSSEKHGRWRSINPYIFPFINWNTAI---------GKNNNQVNENASYVQDSNLLTSVVSKPELFIQATAVESFYKELLSVHGSNAAELLTFISKEIISQAENMLTFRIREKAEQGNHSTTVAIESTGSDLKSKVSSRQGTGKQLLTRLCLIIASLKLGAQRRIVSAV-SRFPAELVAVRLVRPLHSIIDSELNEVRRITERAIAATRV-LGLFYAASELNGWRY----------------AILPRQEFYNHSVSDLVD--LKEDYNRWLYSMENSSSGKSLFSFCAHSYVLDEAAKARVLHAEA----REAMKSEVVRSLWMNSQAVVVGPWGIPFIAGTDSPYCYLHVRRASLVQDALSVISNADPRELRKPLRVVFLGEEGVDEGGLQKEFFQLITAELFSADYGMFVYNEKTRFHWFRVDSLEVDD--NFKLVGIIIGMALYNGVILDLHFPLLVYKKLLNWSPDLDDVKEAFPEIGNSLQAMLDYEED-----------DMEQVFMTTFAIQYEFFGELRTWELVPGGIQIYVNQSNKEDFIRRYIEYLTDVSIHSQFDAFSKGFFWMLSGPALKIFRPDELEVLVCGQRHLDFEALKQVTVYEGGFNAESQVIKWFWDIVDEMTLEEKLHLLFFVTGSDRAPVGGLGQLRFVIQRAGPDTDRLPTSHTCFNILLLPEYSSKEKTRNRLLTAIQNAQGFGLQ 1119          

HSP 2 Score: 102.064 bits (253), Expect = 5.082e-18
Identity = 72/222 (32.43%), Postives = 112/222 (50.45%), Query Frame = 0

Query:  289 PVQSWYSRKNVVNLCSTLYHTVAVTLTGEVYGAGVNEDGQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHS----ADAASSQRVGPRTVV--GLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGANRFQAERVK--ALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEVVQEPQQV 502
            PV++    K VV + +  +H +A+T  G+VY  G+   GQ      E  ++ P LVE L +Q V  ++ G+NH+  +T++G   +FG+N+ GQLG S     ++ +   +  R  +   L S      ACG   TL LTS G+V +CG G+ G    G N+ +   V   +L    +  +A GA H+ A++  G L+ WG N  GQ G      V   P Q+
Sbjct:  147 PVKN-LQNKRVVKVAAGAFHWLALTDAGQVYSCGLGRHGQTGLGNCE-DVYEPCLVEALWAQPVVNIAAGENHSIVITSTGFAFAFGSNKYGQLGISRQRLPESETRAELPLRIYMPQELASERFLAAACGTHHTLFLTSNGKVLACGSGQDGVLGIGNNKDEVCLVVIFSLFEYTIVQIAAGARHSAALSSQGELFLWGSNEQGQLGFVEPTCVFSLPTQM 366          

HSP 3 Score: 85.1149 bits (209), Expect = 7.553e-13
Identity = 71/264 (26.89%), Postives = 116/264 (43.94%), Query Frame = 0

Query:  371 VVSFGNNEVGQLGHSADAASSQRVGPRTVV--GLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGA-NRFQAERVKA-LRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEVV-QEPQQVLETAGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLG 629
            +V +G N+ GQLG + D  + Q    + +V  G+   +V+ +ACG+  T++  +   ++  G    G +   A NR +  R+++         +A G    +  T +G L  WG   +GQ G GA ++     P + L+   V   A GAFH + L + G+V++CG    GQ GL + E V                                      + P LV  L    V  +A G+ H++V+T  G  ++ GSN +GQLG
Sbjct:   18 LVGWGRNDSGQLGSTIDDENQQVNKAQVLVDFGIAGDVVQSIACGFSSTVVCFNDNSLYVNG----GIYNQTATNRQEPIRIQSPYTRERTQSIACGEDFVILATQSGTLVTWGGGAYGQLGRGAPMDWSGPHPVKNLQNKRVVKVAAGAFHWLALTDAGQVYSCGLGRHGQTGLGNCEDV--------------------------------------YEPCLVEALWAQPVVNIAAGENHSIVITSTGFAFAFGSNKYGQLG 239          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|1210530258|dbj|GAX11174.1| (ubiquitin-protein ligase E3 A [Fistulifera solaris])

HSP 1 Score: 443.351 bits (1139), Expect = 2.196e-129
Identity = 263/631 (41.68%), Postives = 360/631 (57.05%), Query Frame = 0

Query:  824 LEAVYVRAMKLNSPPLLSRLV----QAMSGALDNLVSDAKNLADPDSLRCLLAYWQC-PLNSNTSLSKD----TFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLRINFGKG---------RAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGG-PVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSK-EKLKERLMKAISECRGFGLQ 1434
            L+ +++   KLN+  L   L     +++   L +L      +  P+S+RCLL Y +   LNS   +  D      + LCET++ LP  G   LL ++   YP  LF   L++PL + +   L I   +          RAVPI+V  + +L  ASE  +                    + +P+ F + AV  +P E L  DLR+ K+    K+     F  C  PFL+    KR LL  E+ +   + A +    ++   G  + P+ VL V+R +LL  TLA++ + D   L+ +L+V F  E+GVD GGV +EFF LL+ +LFD +  M+         WFNS  T   D Q Y+LVGIL GL LYN VLLD+HFP  VY+KLL  P+GL DL  ++PE+ +G+ +LLDY+ D            VED+FCL+FEV W++  E K+ ELKPGG DI VT+ N+ EYV LYV+W+L +SI   +EAFK G   +M  SS+ L RPEEL LLV GTP L+   LE  A+YEGGY  E   ++  W  +   DL ++  FL F TGS+KAPIGGLG L   +QRAG DSDHLPT+HTCFNTLLLP+YGS  EKL +RL +AI EC GFGL+
Sbjct:  703 LDHLFLELTKLNNKELQKSLADVAEKSIQQGLQSLSKSQARMIYPESVRCLLHYIRFFDLNSRHGVDFDPRGSCVMALCETLLGLPYEGFKALLVWMTL-YPSDLFVRMLVKPLLSQLGKALHIEIDENGVVHTKVSRRAVPIIVSVLRWLHRASERAN--------------------IAAPEDFYSEAVEKMPLEVLFDDLRSLKSYG--KDPVSRPFLICASPFLIPPTKKRDLLLIESQVNMMKTATESGRVEIRPDGLAIDPFYVLEVERENLLEQTLAKIKEADPKELRMKLRVKFKGEDGVDAGGVTREFFHLLSDELFDVNSSMWTRRFGDNITWFNSDCT--WDEQGYELVGILFGLGLYNGVLLDVHFPQAVYRKLLDLPLGLEDL--VDPEIRKGMQQLLDYDGD-----------DVEDIFCLSFEVSWMDLGEEKKVELKPGGADIPVTSFNKEEYVLLYVKWLLVDSIQPQWEAFKKGVLRIMESSSLDLFRPEELELLVVGTPELDFEALEKNAEYEGGYTKESDAVQNLWKYLKSADLDTKMKFLQFSTGSSKAPIGGLGSLQFKVQRAGIDSDHLPTSHTCFNTLLLPDYGSNYEKLCDRLGRAILECEGFGLE 1295          

HSP 2 Score: 135.191 bits (339), Expect = 4.635e-28
Identity = 117/409 (28.61%), Postives = 176/409 (43.03%), Query Frame = 0

Query:  308 HTVAVTLTGEVYGAGVNEDGQVSP--DRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGH----SADAASSQRVGPRTV-VGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGANR----FQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEV-----VQEPQQVLET------------------------AGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQSFAVGLGTG 676
            H+   T  GEV+  GVN  G V P   R   ++  P  +E L +  +  VSCG +HTA +T  G V+++G+NE GQLGH      D    +   P+ + +G G+R V  VACG  FTL+LTS+  V +CGV    G+   A +      AE+  AL  +P++ +A G  HA+ +T  G  +AWG N  G  G G   E+     V  P   LET                          V  AA G  H+V++   G +  CG N +GQLG+   E +      +R+E                                +VR  E  +      G  +TL+L  +G+VW +G+ + G+L                   ++V  L    +  ++AGG+Q+ A+    G
Sbjct:  284 HSAYATNQGEVFACGVNTAGAVDPLHPRDVTEIVRPSKLECLGTAFIRHVSCGWDHTAAITDMGVVLTWGSNEKGQLGHFRSVDQDNGLKEWCSPKAMFLGPGTRAV-SVACGNQFTLVLTSRMSVLACGVSTVAGYRVNAKQGDLGLPAEQ-PALVGLPLAAVAAGKDHAVVLTAHGSAYAWGMNTSGCCGRGYPTELLSPVPVLTPTSQLETPLGKTPFPNWSVSENNSKISLADDVAVVHAAAGNDHTVLVTRSGTLLVCGSNSKGQLGVSGKEII------NRVE--------------------------------IVRHPENGRFISAEAGCENTLLLDGSGDVWQMGATTGGEL-------------------KKV--LSGKNILTISAGGDQNVAIAAEKG 631          

HSP 3 Score: 67.781 bits (164), Expect = 1.853e-7
Identity = 67/241 (27.80%), Postives = 96/241 (39.83%), Query Frame = 0

Query:  407 VKQVACGYLFTLLLTSQGEVFSCGVGECGG-----HADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAG------AGAGVEVVQEPQQVLETAGVR--SAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPS 634
            V  V+CG   +   T+QGEVF+CGV   G        D     +  +++ L    +  ++ G  H  AIT  G +  WG N  GQ G         G++    P+ +    G R  S ACG   +++L     V ACG                S+  G R+              + GD+  G PA      P LV     L +  VA G  H +VLT  G  ++ G N+ G  G G P+
Sbjct:  275 VLSVSCGPCHSAYATNQGEVFACGVNTAGAVDPLHPRDVTEIVRPSKLECLGTAFIRHVSCGWDHTAAITDMGVVLTWGSNEKGQLGHFRSVDQDNGLKEWCSPKAMFLGPGTRAVSVACGNQFTLVLTSRMSVLACGV---------------STVAGYRVNA------------KQGDL--GLPAEQ----PALVG----LPLAAVAAGKDHAVVLTAHGSAYAWGMNTSGCCGRGYPT 478          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|1210514183|dbj|GAX26965.1| (E3 ubiquitin-protein ligase HERC4 [Fistulifera solaris])

HSP 1 Score: 437.958 bits (1125), Expect = 2.227e-127
Identity = 259/631 (41.05%), Postives = 359/631 (56.89%), Query Frame = 0

Query:  824 LEAVYVRAMKLNSPPLLSRLV----QAMSGALDNLVSDAKNLADPDSLRCLLAYWQC-PLNSNTSLSKD----TFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLRINFGKG---------RAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGG-PVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSK-EKLKERLMKAISECRGFGLQ 1434
            L+ +++   KL +  L   L     +++   L +L      +  P+S+RCLL Y +   LNS   +  D      + LCET++ LP  G   LL ++   YP  LF   L++PL + +   L +   +          RAVPI+V  + +L  ASE  +                    + +P+ F + AV  +P E L  DLR+ K+    K+     F  C  PFL+    KR LL  E+ +   + A +    ++   G  + P+ VL V+R +LL  TLA++ + D   L+ +L+V F  E+GVD GGV +EFF L++ +LFD +  M+         WFNS  T   D Q Y+LVGIL GL LYN VLLD+HFP  VY+KLL  P+GL DL  ++PE+ +G+ +LLDY+ D            VED+FCL+FEV W++  E K+ ELKPGG DI VT+ N+ EYV LYV+W+L +SI   ++AFK G   +M  SS+ L RPEEL LLV GTP L+   LE  A+YEGGY  E   ++  W  +   DL ++  FL F TGS+KAPIGGLG L   +QRAG DSDHLPT+HTCFNTLLLP+YGS  EKL +RL +AI EC GFGL+
Sbjct:  715 LDHLFLELTKLKNMELQKSLADVAEKSIQQGLQSLSKSQARMIYPESVRCLLHYIRFFDLNSRHGVDFDPRGSCVMALCETLLGLPYEGFKALLVWMTL-YPSDLFVRMLVKPLLSQLGKALHVEIDENGVVHTKVSRRAVPIIVSVLRWLHRASERAN--------------------IAAPEDFYSEAVEKMPLEMLFDDLRSLKSYG--KDPASRPFLICASPFLIPPSKKRDLLLIESQVNMMKTATESGRVEIRPDGLAIDPFYVLEVERENLLEQTLAKIKEADPKELRMKLRVKFKGEDGVDAGGVTREFFHLVSDELFDVNSSMWTRRFGDNITWFNSDCT--WDEQGYELVGILFGLGLYNGVLLDVHFPQAVYRKLLDLPLGLEDL--VDPEIRKGMQQLLDYDGD-----------DVEDIFCLSFEVSWMDLGEEKKVELKPGGADIPVTSFNKEEYVLLYVKWLLVDSIQPQWDAFKKGVLRIMESSSLDLFRPEELELLVVGTPELDFDALEQNAEYEGGYTKESEAVQNLWKYLKSADLDTKMKFLQFSTGSSKAPIGGLGSLQFKVQRAGVDSDHLPTSHTCFNTLLLPDYGSNYEKLCDRLGRAILECEGFGLE 1307          

HSP 2 Score: 139.043 bits (349), Expect = 2.406e-29
Identity = 116/412 (28.16%), Postives = 175/412 (42.48%), Query Frame = 0

Query:  307 YHTVAVTLTGEVYGAGVNEDGQVSP--DRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGH----SADAASSQRVGPRTV-VGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGH------ADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEV-----VQEPQQVLET------------------------AGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQSFAVGLGTG 676
            YH+   T  GEV+  G+N  G V P   R   ++  P  +E L +  V  VSCG +HTA +T  G V+++G+NE GQLGH      D    +   P+ + +G G+R V  VACG  FTL+LTS+  V +CGV    G+      +D      AE+  AL  +P++ +A G  HA+ +T  G  +AWG N  G  G G   E+     V  P   LET                          V  AA G  H+V++   G +  CG N +GQLG+   E +      +R+E                                +VR  E  +      G  +TL+L ++G+VW +G+ + G+L   +  +                      +  + AGG+Q+ A+    G
Sbjct:  293 YHSAYATNQGEVFACGINTSGAVDPLHSRDIAEIVRPSKLECLGTAFVRHVSCGWDHTAAITDMGVVLTWGSNEKGQLGHFRSSDQDNCLKEWCSPKAMFLGQGTRAVS-VACGNQFTLVLTSRMSVLACGVPTVAGYRVNLKQSDLGLNLPAEQ-PALVGLPLAAVAAGKDHAVVLTAHGSAYAWGLNTSGCCGRGYPTELLSPVPVLTPTSQLETPLGKTPFPNWSVSENNSKISLADDVAVVHAAAGNDHTVLVTRSGTLLVCGSNSKGQLGVSGKEII------NRVE--------------------------------IVRHPENGRFISAEAGCENTLLLDDSGDVWQMGATTSGELKKVLSGK---------------------NILTITAGGDQNVAIAAEKG 643          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|552839812|ref|XP_005849888.1| (hypothetical protein CHLNCDRAFT_21324 [Chlorella variabilis] >gi|307109548|gb|EFN57786.1| hypothetical protein CHLNCDRAFT_21324 [Chlorella variabilis])

HSP 1 Score: 409.068 bits (1050), Expect = 4.699e-126
Identity = 214/458 (46.72%), Postives = 288/458 (62.88%), Query Frame = 0

Query:  983 GSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGG--PVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLD----EEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGLQ 1434
            G +I   +F N  +S     +L  +   W+    +      + + C+ P+LL+ EAK  +++GEA ++QQQ      M+  F GG   V  +L +RV R+H++   L Q+   ++  LKK L+V+F+     E   DEGGV KEFFQLLT  LF   Y MF   E  R  WF+ +     D  EY LVG ++GLA+YN+V+LD+HFPL VYKKLLG     +DL    P+L  GL +LLD+E D            VE VFC +F   +  + E++  ELKPGG  I VTAANR EYVDLY  W+L  S+ + F AF+AG+  V GG +++L  P EL LLVCG PHL+   L+AVAKYEGGY AEH  ++ FW V+H + L  +R FLMF TGS +AP+GGLG+L LLIQRAGPDSD+LPT+HTCFN+LLLPEY SK++L+ +L+ A+   +GFGLQ
Sbjct:   17 GGIIPYTEFHNAQLSE--HANLQAEYVAWR----QNTSTTALCSICQMPYLLTPEAKSFIMHGEAAMQQQQHLSAAAMQAFFSGGSAAVAAFLQIRVRRSHVVEDALNQLAHLEAQDLKKPLRVTFITGGVPEPAQDEGGVTKEFFQLLTRDLFRPEYAMFTYNELTRTHWFSPASLEAED--EYALVGAVLGLAIYNAVILDVHFPLAVYKKLLGIRPTFADLKAAFPDLGRGLQQLLDFEGD------------VEAVFCRSFTAQYEFYGEMREEELKPGGASIPVTAANRREYVDLYTAWLLEGSVGRQFGAFRAGFLRVCGGPALTLFTPAELELLVCGLPHLDFEALQAVAKYEGGYSAEHQEVRWFWEVLHSLSLEQKRAFLMFTTGSDRAPVGGLGKLPLLIQRAGPDSDNLPTSHTCFNSLLLPEYASKDRLRAKLLTAVENAQGFGLQ 454          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|470527595|ref|XP_004357184.1| (o ubiquitin protein ligase E3A [Acanthamoeba castellanii str. Neff] >gi|440804151|gb|ELR25029.1| o ubiquitin protein ligase E3A [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 402.519 bits (1033), Expect = 1.614e-120
Identity = 234/600 (39.00%), Postives = 330/600 (55.00%), Query Frame = 0

Query:  846 AMSGALDNLVSDAK----NLADPDSLRCLLAYWQCPLNSNTSLSKDTFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEY-HLRI-------NFGKGRAVPILVVTMSYLFMASEGGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVKEEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGL 1433
             ++ A + LV+  K     L+ P+SLR ++   Q P+  +    K     L   +  LP   + +L+      +   + A R+   L    +Y  LRI       +      +P  V  +  L+ A+E               T+DK     +   +F N A++   D  L  D   W         N   F+FC Y F+  A  K ++L  E+++EQ    Q+ + R +F G    P LV ++ R HL+  TL+ + + D   LKK+L+V F+ EE +DEGGV+KE FQL+  Q+ D  +GMF  +E  R  WFNS  T F    E++L+GI++GLA+YN ++LDLHFP VVYKKL+G    L DL ++ P    GL KLL+++              VE+ F  NF++ +  +  V+ H+LK GG DI +T  NR EYVDLYV+W L +SIA  FEAF +G+  V    +  + R EEL  LVCG+P L+  ELE V +Y+ GY  +H VIK FWS+VH M L  ++  L F TGS ++PIGGLG+LA +I R G DSD LP AHTCFN LLLPEY S+EKL+ERLM AIS   GFG+
Sbjct:  146 VLASAFERLVASLKVAAPRLSQPESLRQVVIVLQSPMLLDLDYHKSILGPLIRAIPLLPERSKDVLV-----SWWSQMSAERMRTFLGIVQQYITLRILAAPHAFSLHNDDYIPAGVRVLQLLYTANER-------------ITKDK-----LDYPEFYNDAINESID--LKEDFMRWID-------NSGQFSFCNYSFVFDAGTKSKILQYESIVEQHHQRQEALRRTIFTGMVSAPALVFKIRREHLIQDTLSAIQRHDPADLKKELRVHFVGEEAIDEGGVQKELFQLILRQILDAKFGMFTYSEEARTSWFNSVSTDFD---EFKLIGIILGLAIYNGIILDLHFPFVVYKKLVGLEPTLDDLKDVNP----GLRKLLEFD------------GNVEETFMQNFQISYEAYGMVQTHDLKEGGADIPLTNDNRQEYVDLYVRWYLKDSIAAQFEAFLSGFRLVCESRAFDMFRAEELEQLVCGSPVLDFEELEKVTQYDNGYHQDHRVIKDFWSIVHSMTLEEKKRLLFFSTGSDRSPIGGLGKLAFVITRHGEDSDRLPQAHTCFNHLLLPEYASREKLQERLMTAISNAEGFGM 694          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|971509004|dbj|GAQ89192.1| (Putative ubiquitin-protein ligase [Klebsormidium nitens])

HSP 1 Score: 409.068 bits (1050), Expect = 5.853e-118
Identity = 242/619 (39.10%), Postives = 339/619 (54.77%), Query Frame = 0

Query:  821 VQGLEAVYVRAMKLNSPPLLSRLVQAMSGALDNLVSDAKNLADPDSLRCLLAYWQCPLNSNTSLSKDTFLKLCETVVSLPLSGRLLLLRFVRADYPPHLFATRLLRPLHTHIEYHLRINFGK---GRAVPILVVTMSYLFMASE-GGSWETTSTESGPWKTEDKQCGSLISPDQFANTAVSALPDESLLHDLRTWKAVK-EEKNMNRNVFTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGLQ 1434
            V+ ++ VY R +KL    ++ RL  +    LD L + A  +      +  L   Q PL     L  D   +L   +  LP       +R+ R  YP  +FA R +R    +I+    +          +   V+ +  L+ A+  GG  E                  ++    F +  VS     +L  + R W+ V+    +  + + ++C+ P+LL+ EAK  +L GEA L++ +      + Q+F GG   P+L+L+V R+H++   LAQ+G   +  LKK LKV F+ EE +DEGGV KEFFQL+  +LFD  +GMF   +  R++WF  S TS     E+ LVGI++GLA+YNSV+LD+H PLVVYKKLLG    L DL +  P +   L KLLD+E D            VE  F L+F+V +  F     H+L   G    VT ANR +YV+LYV++VL ESI   F+AF+ G+  V GG ++SL R EEL LLVCG PH +   L   A+YE G+  + PVIK FW +V  M L  ++  L F TGS++APIGGL  L  ++QR GPD+D LPTAHTCFN LLLPEY +KEKL +RL  AI    GFGLQ
Sbjct:  603 VEAIKRVYDRILKLYDTEMIQRLGNSTVRLLDGLAAHAATVPSERWAKIPLILMQSPLLGMKGLG-DLSSRLFGLIAQLPHEVHFAYVRWFRT-YPKDIFAGRFVRSAKNYIDTKQNMIAASEPPADDIAAAVMVLRLLYKANTLGGRPE------------------ILPYADFHSITVSQ--KAALREEYRRWRQVEGAPVSRTKRLVSYCQVPWLLTPEAKSEVLQGEANLQKTRELHNWQLGQIF-GGNSAPFLILQVRRSHIVPDALAQLGH-HTFDLKKPLKVVFVGEEAIDEGGVTKEFFQLVVRELFDVRFGMFTYDDESREFWF--SQTSMESESEFHLVGIILGLAIYNSVILDVHLPLVVYKKLLGGKPTLEDLKDARPAVGRSLQKLLDFEGD------------VESAFGLDFQVSYENFGAEVIHDLITNGGQTPVTNANRQQYVELYVKYVLEESIQTQFDAFRRGFLQVCGGPALSLFRHEELELLVCGLPHFDFDALSKEARYEAGFTKDSPVIKWFWELVKAMSLDEKKKLLFFTTGSSRAPIGGLSELRFVVQRMGPDTDRLPTAHTCFNVLLLPEYATKEKLADRLHLAIKNAEGFGLQ 1183          

HSP 2 Score: 152.525 bits (384), Expect = 1.778e-33
Identity = 118/384 (30.73%), Postives = 182/384 (47.40%), Query Frame = 0

Query:  295 SRKNVVNLCSTLYHTVAVTLTGEVYGAGVNEDGQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECG--GHADGANRFQAERVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAGAGVEVVQEPQQV--LETAGVRSAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQL-EPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGL--DKARVFMVAAGGEQSFAV 671
            + +NVV+  +  YH+  +T  GE+Y +G N+ GQ+    +E     PV V  L +  +T V+CG  HT  +T  GA+ ++G  + GQLGH     S+  V P+ V G       +VACG   TL LT  GEV+S G G+ G  GH D   +     V +L  + +  +A G +H+ A+TV G+++ WG+N++GQ G   G  + + P ++  L   GV   ACG  H++ +   G ++  G+   GQ G  +T+   S                                      PTLV  L + ++V   + G  HTL + E G+V+  G     QLG G+        S  Q    ++ GL  DK  +F+V AGG+ + AV
Sbjct:  102 AERNVVHAAAAEYHSAFLTSDGELYTSGSNDTGQLGVKGRESAA-EPVRVAALDTYTITHVACGRGHTVAVTDQGALAAWGEGDQGQLGHRDAMGSTAPVQPKIVKGTRELRFVRVACGAAHTLALTGSGEVYSFGQGQLGALGHGDLETKPTPTLVDSLWGLAIVQVAAGENHSAALTVDGQIFTWGRNKYGQLGHSDGGTICR-PTRLNGLSNEGVVHVACGGDHTLAITRKGELYTWGRGRWGQCGHGTTDDTFS--------------------------------------PTLVTGLPKGVRVAHASAGTRHTLAVLEKGDVYGWGDGELCQLGPGV--------SGVQTTPVKLQGLPQDKRLLFLV-AGGDHNIAV 436          

HSP 3 Score: 98.5969 bits (244), Expect = 6.314e-17
Identity = 73/218 (33.49%), Postives = 108/218 (49.54%), Query Frame = 0

Query:  283 TAPLFAPVQSWYSRKNVVNLCSTLYHTVAVTLTGEVYGAGVNEDGQVSPDRQEPQLWTPVLVEPLLSQRVTMVSCGDNHTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECG--GHADGANRFQAERVKAL-RYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGAG-AGVEVV 496
            TAP+   +         V +     HT+A+T +GEVY  G  + G +     E +  TP LV+ L    +  V+ G+NH+A LT  G + ++G N+ GQLGHS D  +  R  P  + GL +  V  VACG   TL +T +GE+++ G G  G  GH    + F    V  L + V V+  + G  H LA+   G ++ WG     Q G G +GV+  
Sbjct:  197 TAPVQPKIVKGTRELRFVRVACGAAHTLALTGSGEVYSFGQGQLGALGHGDLETKP-TPTLVDSLWGLAIVQVAAGENHSAALTVDGQIFTWGRNKYGQLGHS-DGGTICR--PTRLNGLSNEGVVHVACGGDHTLAITRKGELYTWGRGRWGQCGHGTTDDTFSPTLVTGLPKGVRVAHASAGTRHTLAVLEKGDVYGWGDGELCQLGPGVSGVQTT 410          

HSP 4 Score: 95.1301 bits (235), Expect = 7.195e-16
Identity = 89/317 (28.08%), Postives = 132/317 (41.64%), Query Frame = 0

Query:  361 HTACLTASGAVVSFGNNEVGQLGHSADAASSQRVGPRTVVGLGSRMVKQVACGYLFTLLLTSQGEVFSCGVGECGGHADGANRFQAE--RVKALRYVPVSGLATGASHALAITVTGRLWAWGQNRHGQAGA--GAGVEVVQEPQQVLETAGVR--SAACGAFHSVILGEDGRVWACGKNHRGQLGLPSTEAVGSSEGGDRIEGDGDRMEEGKGDEQDGDIEGGAPAASCYHVPTLVRQLEPLKVCMVACGDAHTLVLTEAGEVWSLGSNSHGQLGTGMPSRASSMASAWQWRAQRVPGLDKARVFMVAAGGEQSFAV 671
            + A L+ +    ++G  ++GQLG   ++       P  V  L  R V   A     +  LTS GE+++ G  + G          AE  RV AL    ++ +A G  H +A+T  G L AWG+   GQ G     G     +P+ V  T  +R    ACGA H++ L   G V++ G+   G LG                                GD+E           PTLV  L  L +  VA G+ H+  LT  G++++ G N +GQLG    S   ++      R  R+ GL    V  VA GG+ + A+
Sbjct:   63 YKALLSRAVFTYAWGRGDLGQLGLGVESNVDV---PTLVKALAERNVVHAAAAEYHSAFLTSDGELYTSGSNDTGQLGVKGRESAAEPVRVAALDTYTITHVACGRGHTVAVTDQGALAAWGEGDQGQLGHRDAMGSTAPVQPKIVKGTRELRFVRVACGAAHTLALTGSGEVYSFGQGQLGALG-------------------------------HGDLE-------TKPTPTLVDSLWGLAIVQVAAGENHSAALTVDGQIFTWGRNKYGQLGH---SDGGTIC-----RPTRLNGLSNEGVVHVACGGDHTLAI 330          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|857972377|emb|CEP01414.1| (hypothetical protein PBRA_002020 [Plasmodiophora brassicae])

HSP 1 Score: 386.341 bits (991), Expect = 1.084e-114
Identity = 200/414 (48.31%), Postives = 261/414 (63.04%), Query Frame = 0

Query: 1025 FTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQ---GGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLG--RLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGL 1433
            F F R P+LL+A  K  L+  +A+L+ +    + +MR +F     GP  P+L+L+V R+ L+  TL Q+   +S   KK LKV F+ E+G+DEGG++KEFFQLL  QLF+ +YGMF + +    +WFN    SF    EY+L+G ++GLA+YNSV+LDLHFPL VYKKLL  PV   DL    P L  GL +L D++ D            VE VFCL F +++  F EVKR EL P G++I VT  NR  YV+L+ ++ L  SI   F AFK G++ V GG+ + L RPEEL L +CG+P L+ H LEA AKYE  Y  + PVI  FW VVHEM    +R FL F TGS + PI GLG  R+  +I R G DSD LPT+HTCFN LLLP Y +  KL+ERL+ AI    GFGL
Sbjct:  283 FCFSRSPYLLNAATKSSLMQFDAMLQMRDQLDEALMRYLFHHSMDGPA-PFLILQVRRSDLVADTLHQIQGRNSDDFKKPLKVKFIGEDGIDEGGIQKEFFQLLVKQLFNPAYGMFIVDDETHTYWFNKD--SFESKHEYELIGAMLGLAIYNSVILDLHFPLCVYKKLLNSPVSFDDLRSFNPSLHRGLQQLRDFDGD------------VESVFCLTFSIEYSLFGEVKRVELCPNGQNIPVTKENRERYVELFTEYTLNTSIETQFSAFKYGFEMVCGGAWLHLFRPEELELAICGSPELDFHALEAAAKYE-NYTRDSPVIVNFWKVVHEMTEEQKRRFLSFCTGSDRVPINGLGSLRVPFIIARNGGDSDRLPTSHTCFNHLLLPAYSTLGKLRERLLTAIQNSEGFGL 680          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|1000780890|gb|KXN72081.1| (HECT-domain-containing protein [Conidiobolus coronatus NRRL 28638])

HSP 1 Score: 389.423 bits (999), Expect = 3.234e-113
Identity = 199/412 (48.30%), Postives = 258/412 (62.62%), Query Frame = 0

Query: 1025 FTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSSGTSFGDPQ---EYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGL 1433
            F+FC Y F+L+   K  +L  E++++ +   Q    R MF G    PYLVL V R HL+   L Q+    S  LKKQL+V F  EEGVDEGGV+KEFFQLL   LF+  YGMFK  E  R  WF S+     D Q   E++LVG L+GLA+YNSV+LDLHFPL +YKKLL  PV L DL E++  L +GL  LL +E D            VE V+   F++++  F +   ++L P G +I +T  NR ++VDLYV +VL +SI K FEAFK G+DHV   + I L RPEE+ L+VCG+  L+   LE V  Y+GG+  + PVIK FW  VHE  +  +R  L F TGS + P+GGLG+L  +I + GPDSD LPT+HTCFN LLLPEY   +KLK RL+ AI    GFG+
Sbjct:  517 FSFCNYAFILNPHTKSDILKVESMVQMRSELQDAFFRAMFVGMNS-PYLVLEVRREHLIRDALFQLVNKASQELKKQLRVQFTGEEGVDEGGVQKEFFQLLIRDLFEPQYGMFKRYEDTRRCWFVSNPNE-EDSQSLDEFKLVGRLIGLAIYNSVILDLHFPLALYKKLLNHPVTLKDLEELDMPLAQGLKGLLQFEGD------------VESVYSWTFQIEYEVFGQRHHYDLIPNGDNIPLTNQNRKQFVDLYVDFVLNKSIEKQFEAFKEGFDHVCANTVIKLFRPEEVELMVCGSSELDFEALEKVTHYDGGFTPDSPVIKYFWQTVHEFTMEQKRKLLFFATGSDRVPMGGLGKLQFVIAKNGPDSDRLPTSHTCFNVLLLPEYRDMDKLKSRLLTAIENSVGFGM 914          

BLAST of EWM28520.1 vs. NCBI_GenBank
Match: gi|754350014|ref|XP_004345557.2| (ubiquitin ligase E3A isoform 1 [Capsaspora owczarzaki ATCC 30864] >gi|765554897|gb|KJE95518.1| ubiquitin ligase E3A isoform 1 [Capsaspora owczarzaki ATCC 30864])

HSP 1 Score: 383.259 bits (983), Expect = 2.074e-112
Identity = 191/411 (46.47%), Postives = 255/411 (62.04%), Query Frame = 0

Query: 1025 FTFCRYPFLLSAEAKRRLLYGEAVLEQQQAAQKDMMRQMFQGGPVMPYLVLRVDRNHLLNSTLAQVGQCDSMTLKKQLKVSFLDEEGVDEGGVKKEFFQLLTAQLFDFSYGMFKMTESKRDWWFNSS--GTSFGDPQEYQLVGILVGLALYNSVLLDLHFPLVVYKKLLGRPVGLSDLGEIEPELMEGLLKLLDYEEDAKNTGEEGAMAKVEDVFCLNFEVDWVEFDEVKRHELKPGGKDIEVTAANRHEYVDLYVQWVLAESIAKPFEAFKAGYDHVMGGSSISLLRPEELMLLVCGTPHLNLHELEAVAKYEGGYEAEHPVIKAFWSVVHEMDLASQRNFLMFVTGSAKAPIGGLGRLALLIQRAGPDSDHLPTAHTCFNTLLLPEYGSKEKLKERLMKAISECRGFGL 1433
            FTFC +PF+++A AK  LL  E+ + Q +         +F      PYL + V R+H++   L Q+   D   LK +L+V F+DEEGVDEGGV+KEFFQL+   +    +GMF+  E  R+ WF  +   T  G   EY LVG+L+GLA+YN V+LDLHFPLV+YKKLLG+PV  +DL   +P L  GL +LL+Y+ D            VE  FC  F++ +  F   K H+LKP G  I VT  NRHE+V LY  W+L  SIA  F  FK G+D V   ++I+L  PEEL +L+CG P+ + H LE   +Y+GG++ + PVI+ FW VVH+M    ++  L F TGS + P+GGL  L L I R G D++ LPTAHTCFN L+L EY +KEKLKERL+ AI    GFGL
Sbjct:  386 FTFCHFPFVITATAKYNLLAEESYMIQSKRHTDAFFNSIFSEAQD-PYLRVLVRRDHIIEDVLLQLSHKDPSQLKHRLRVRFVDEEGVDEGGVQKEFFQLIFRDILAPKFGMFEYEEETRNMWFRKAIDDTLQG---EYVLVGLLIGLAIYNRVMLDLHFPLVIYKKLLGKPVTFADLQVAQPALAHGLQELLEYDGD------------VEATFCRTFQLSYDVFGATKTHDLKPNGDSIPVTNENRHEFVQLYTDWLLNTSIAASFNEFKRGFDMVTADTAITLFSPEELEILICGEPNFDFHSLENATEYDGGFDKDSPVIRFFWEVVHDMSEEEKKRLLFFATGSDRVPVGGLSHLNLTIVRNGTDAERLPTAHTCFNVLMLSEYPTKEKLKERLLTAIENSEGFGL 780