EWM28530.1, cds2147 (CDS) Nannochloropsis gaditana

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|585110948|gb|EWM28530.1| (voltage-dependent l-type ca channel alpha 1 subunit [Nannochloropsis gaditana])

HSP 1 Score: 2560.79 bits (6636), Expect = 0.000e+0
Identity = 1254/1254 (100.00%), Postives = 1254/1254 (100.00%), Query Frame = 0

Query:    1 MWPSHGAGHSEVPIRGGSSRHAVTEGLTPGLESEGDDCISSLPVSSDGPSTPNGSHSDATNSNTSPSHAPGRPWTLPAVDGRAPRRRAMPASPSQAPPRVMVHPFGVFPPQEDSAEVWAIRPRAGSGRTSLGRRRRYDSYVSTLDLDDLRELDDELLRRAIGGEGDIPEEENKNGEDESLGTPRRAGTGTESVHSCPRRERREEDGVENARSSSQRGSTGSHHRRELLCDEISGPQERRSHRQGLGRRAHVDLAGARPCRGIDHNADDSRDCRTRHSMPHFQQQPNIQAQSGHQQRMPGVNPNRLSVSQHHSPFRPHRDSISRGAPPPNPTFYDPKMGALPPAAPNDNELYEEGARTPPASYRHSSSRPALEGGIPQLPCVQGSTSNSRHSRRSSLRQTNDAADGYGASSYRNSRRDSRMSFGIGWLYDHEGRKEGDGERGGGGNEGSRPPSASGRRPNKRTLLHWLDQRLPRMSDEHRIARASLYLSDAIDTRPVRMRNGGTGNLFAYRLSKRTGWLWIMQILAVVHLLLALWEPPRALADSAQYHDSISRHTLTMAASTARHLLGLELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDKRWVEPKEEEENTEAPALTEGLGNSLPSRNGKEEERRFDAPPGAGASYDGEDSYEGGQSWHQPTTGPNRMGPVPSPTPGDDLEFQRRRRAGGDMGGGGQFFHASVI 1254
            MWPSHGAGHSEVPIRGGSSRHAVTEGLTPGLESEGDDCISSLPVSSDGPSTPNGSHSDATNSNTSPSHAPGRPWTLPAVDGRAPRRRAMPASPSQAPPRVMVHPFGVFPPQEDSAEVWAIRPRAGSGRTSLGRRRRYDSYVSTLDLDDLRELDDELLRRAIGGEGDIPEEENKNGEDESLGTPRRAGTGTESVHSCPRRERREEDGVENARSSSQRGSTGSHHRRELLCDEISGPQERRSHRQGLGRRAHVDLAGARPCRGIDHNADDSRDCRTRHSMPHFQQQPNIQAQSGHQQRMPGVNPNRLSVSQHHSPFRPHRDSISRGAPPPNPTFYDPKMGALPPAAPNDNELYEEGARTPPASYRHSSSRPALEGGIPQLPCVQGSTSNSRHSRRSSLRQTNDAADGYGASSYRNSRRDSRMSFGIGWLYDHEGRKEGDGERGGGGNEGSRPPSASGRRPNKRTLLHWLDQRLPRMSDEHRIARASLYLSDAIDTRPVRMRNGGTGNLFAYRLSKRTGWLWIMQILAVVHLLLALWEPPRALADSAQYHDSISRHTLTMAASTARHLLGLELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDKRWVEPKEEEENTEAPALTEGLGNSLPSRNGKEEERRFDAPPGAGASYDGEDSYEGGQSWHQPTTGPNRMGPVPSPTPGDDLEFQRRRRAGGDMGGGGQFFHASVI
Sbjct:    1 MWPSHGAGHSEVPIRGGSSRHAVTEGLTPGLESEGDDCISSLPVSSDGPSTPNGSHSDATNSNTSPSHAPGRPWTLPAVDGRAPRRRAMPASPSQAPPRVMVHPFGVFPPQEDSAEVWAIRPRAGSGRTSLGRRRRYDSYVSTLDLDDLRELDDELLRRAIGGEGDIPEEENKNGEDESLGTPRRAGTGTESVHSCPRRERREEDGVENARSSSQRGSTGSHHRRELLCDEISGPQERRSHRQGLGRRAHVDLAGARPCRGIDHNADDSRDCRTRHSMPHFQQQPNIQAQSGHQQRMPGVNPNRLSVSQHHSPFRPHRDSISRGAPPPNPTFYDPKMGALPPAAPNDNELYEEGARTPPASYRHSSSRPALEGGIPQLPCVQGSTSNSRHSRRSSLRQTNDAADGYGASSYRNSRRDSRMSFGIGWLYDHEGRKEGDGERGGGGNEGSRPPSASGRRPNKRTLLHWLDQRLPRMSDEHRIARASLYLSDAIDTRPVRMRNGGTGNLFAYRLSKRTGWLWIMQILAVVHLLLALWEPPRALADSAQYHDSISRHTLTMAASTARHLLGLELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDKRWVEPKEEEENTEAPALTEGLGNSLPSRNGKEEERRFDAPPGAGASYDGEDSYEGGQSWHQPTTGPNRMGPVPSPTPGDDLEFQRRRRAGGDMGGGGQFFHASVI 1254          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|923121548|ref|XP_013754245.1| (hypothetical protein AMSG_09817 [Thecamonas trahens ATCC 50062] >gi|906560622|gb|KNC53865.1| hypothetical protein AMSG_09817 [Thecamonas trahens ATCC 50062])

HSP 1 Score: 192.2 bits (487), Expect = 9.101e-46
Identity = 178/731 (24.35%), Postives = 319/731 (43.64%), Query Frame = 0

Query:  457 RPNKRTLLHWLDQRLPRMSDEHRIARASLYLSDAIDTRPVRMRNGGTGNLFAYRLSKRTGWLWIMQILAVVHLLLALWEPPRALADSAQYHDSISRHTLTMAASTARHLLGLELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASI------PYL----------NFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASE-WHQRERARFRAILGSRWVEVGSMALLILTCFFLTFY------GSPSISKLSLRRF-SYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFFLSSFSLCVLIS-YSWAVLGMALFAKFGDEIPTAIHQELVGWASVD---NFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDKRWVEPKEEEE 1159
            R  +R    W   R    S+  RI RA+L++ DA+ +RP++    G G + AYR      + WI  ++ V+H+ LA +E P+A A+ A            + A     LLG+EL  V ++ LD  L +YALG+  +                       W  F   +V+L   D+ AAL     S RF+R +R + L+  + +LK         + +L D+ +   + L  F+++G+ +F       +++      PY           NFD+   + L+L VL++T+N+  ++ PA T     +SP Y  FF+ F+LI +  L  +    +F  Y+     +   +R+ ER  ++  F+ LD  R G++    + +++   +P         E+  F      +F+ +       +   EF  + E++  +       ++   +   E W  R  AR   ++G  ++E  +    I  C F+  +       S S  +L++  F  Y  + +F+ E V+K+   G   +     N  DL IV  S +++ +   +              +  LF  LR VRL      F    + F  L + +   + CV+++ YS+A+LGM LF   G   P+ +       AS D   +F +F    + LFQ+ TT +W+ IMY          +A+YF++F +V  ++   +  A+  + F     +  VE +E ++
Sbjct:  332 RQRRRAAAFWQQTRRTVTSELERIQRAALFIEDAVSSRPIKATRLGAGPVAAYRFYHNKVYRWIFTVVVVIHMALAFFEAPQAGAEPA------------VGARARSGLLGIELACVCVYWLDTLLSLYALGIRQY-------------------AQSWWHLFHATVVVLMAIDVIAAL--AGASVRFTRVVRWVPLVLNTVSLKHLLYCTFHTVAKLYDMMVIMCVLLVSFAIVGVQMFGVNTVSPSALGTGNSNPYRDEATGKPLDGNFDNMGEALLSLLVLLSTENYPDIMYPALT-----TSPAYAIFFILFILIGIFLLFSVLTAIIFDTYKEIHEVRVIAARVEERKGLIAAFQILDKNRTGYLHTNQWVALMAEWQPSM-----SMERACF------LFKTMDHDGDNTIDLLEFFDILEIMRYE-------IEEMALVTEETWWSRLGARRVRVVG-EFIESKAFIWTINACIFINVFILCLYRSSMSQDELAVYEFLDYIFIGVFSFEQVVKVVGLGRTEYWIDVWNRFDLTIVVFSLVAVGLQVASNVEKSAGSILVSLRILRLFRTLRTVRLLNTARQFETLMATFLHLGYVVLPITACVVVAFYSYAILGMELFG--GKMSPSVV-------ASADPYTDFGTFPRTLLALFQLFTTSNWHEIMYTARE-GAGKVSALYFVSFFIVMILLFNNILLAIVFEIFT--FQRTMVEQEERKK 993          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|514700088|ref|XP_004997612.1| (hypothetical protein PTSG_01632 [Salpingoeca rosetta] >gi|326433084|gb|EGD78654.1| hypothetical protein PTSG_01632 [Salpingoeca rosetta])

HSP 1 Score: 175.637 bits (444), Expect = 1.455e-40
Identity = 188/709 (26.52%), Postives = 299/709 (42.17%), Query Frame = 0

Query:  458 PNKRTLLHWLDQRLPRMSDEHRIARASLYLSDAIDTRPVRMRNGGTGNLFAYRLSKR-----TGWLWIMQILAVVHLL--LALWEPPRALADSAQYHDSISRHTLTMAASTARHLLGLELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAAS----IPYL--NFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRA----ILGSRWVE----VGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPW-AAMYFLAFHLVANIVVYQLTAALFIDAFL 1144
            P K  L+H  D  +        IA A  ++ DA+  R   + NG     F   L +R     T  LW+  + AV  LL  L  +EPP A        DS    TL           GLE   +++F  D+ +++Y +G  A++  N                   W + ++  V LF  D      + N + RFSR +RP  L+     L+    ++  +L +L  V      F+GLF ++G+ +F+E  Y   S    I  L   FD+FL +FL L+VL TT+N+  ++ PAY    Q  S  + FFF AFV   V FL  + L  V   Y ++  +Q +  R  E   ++  F  LDP   G++ ++++  ++RY+RPD    Q  +            FE L R    R+   +FL + E++L    P   +     M   +W      RFR     I  S W             ++ C +        ++ L      +++V  F  E+VL I +     +++   NV DL I+ +   +LL H L+           +A  CLLF+ L    L+ +          + ++   L    +  ++ Y + V G  LF  +    PT  +    G      F S   A  TLFQV TT +W   M     +    W +A+YF+ F ++ N+V+  L  A+ I+AFL
Sbjct:  594 PTKTNLMHCKDAPV-------YIALACCFIDDAMHGR---LANGKNKMYFEKWLVQRIFLVHTSQLWLHFVYAVAILLATLTFFEPPSA--------DSFKPWTL-----------GLEFFCIMVFITDIGMKMYYMGAMAYWSKN-------------------WHKMQIVFVCLFLLDAVIFSITGN-TIRFSRFVRPAILLGRHRELRHTYFIISAMLPRLAKVFTMMFAFIGLFGIIGIHIFAEE-YTGVSNDEEISQLEGTFDNFLRAFLRLFVLFTTENYPYVVVPAY----QRDSATFIFFF-AFVYAGVFFLTAMLLGLVVSTYFDYTAKQIHAERKKEWRGLMRAFALLDPDGNGYVTLSTWFLLMRYLRPDIDRKQAKF-----------FFELLDRDGNNRLDCFDFLDLREIMLLKIRPTPASELNTTMQRRQW-----TRFRILAFNITTSSWFHPAILAALAINALVACVYHVSITQTELAALQSVHVVFALV--FVAEIVLTIVSEDWYTYIASKWNVADLFIIPS---ALLCHCLSFVSFEWGHFFTLAGNCLLFVRL----LWVSRDARFGLVLIWNIIEAVLHLSYIMAVVMYLFGVAGTELFHGYTPADPTTAYYFDFGCNM--GFRSMGCACFTLFQVMTTSNWQEPMNDL--IVEAGWGSAVYFVLFFIMINLVLTDLLVAVTIEAFL 1218          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|923125662|ref|XP_013756299.1| (hypothetical protein AMSG_12060 [Thecamonas trahens ATCC 50062] >gi|906558025|gb|KNC51268.1| hypothetical protein AMSG_12060 [Thecamonas trahens ATCC 50062])

HSP 1 Score: 117.472 bits (293), Expect = 5.157e-23
Identity = 170/701 (24.25%), Postives = 281/701 (40.09%), Query Frame = 0

Query:  467 LDQRLPRM----SDEHRIARASLYLSDAIDTRPVRMRNGGTGNLFAYRLSKRTGWLWIMQILAVVHLLLALWEPPRALADSAQYHDSISRHTLTMAASTARHLLGLELVL----VLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFS-ETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSP-------SISKLSLRRFSYS--------------IVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALA-PPHSPLHQSEQIANL-CLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFF----------LSSFSLCVLISYSWAVLGMALFAKFGDE--IPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAF 1123
            LD RL       S E  +ARA+ Y+  A+  R V++          Y L K   +LW +++  ++ L L L EP              SR T   A    +  L  E+++    V +  LD+ +  Y  G+  F                     + W      +V +   DI   L S   S RFSR  RPL LI  S  L++  SL+++ +  +  V L   +    ++ LG++LF  +  Y    IP  +F+ +  + L+L+ L+TT+N+  ++ PA+  +   S+     +FM+F+ +  + +A L L  VF  Y    R     S + E+  +++ F C+D    G++DV+ +  +LR +RPD    Q           +  MF  L     G +   EF  + +++   +                    ER   R +    W +V S+  + L   +L   G P       +I   ++    Y                V+ FTL L +K+   G   F S   N+ D  ++G ++I  + +     PH          N+ C L L L  VR   N  I +      R   F            S   +  L+ YS+A++GM LFA   D+   P   ++E        NFN F  A +T FQV  T ++  I     + T  P+ + YF++ 
Sbjct:  112 LDSRLHEALLVDSKEEALARAAHYIDVALTERKVQLGGLTRFQRNLYLLRKNWMYLWFIRLNILLQLGLVLIEPA-------------SRRT---AEDNHKQFLA-EVIIGSFSVFIQLLDVWIRAYPFGLSHF--------------------KEPWNSVNAAVVCINGLDIIFRL-SRVSSFRFSRAARPLLLIAQSRRLRQTFSLIVKSMPAVLGVGLIFFVITACYAHLGVMLFQGQYSYREDEIPAGDFNTWSAAMLSLFFLITTENYPMIMYPAFDVSQYASA-----YFMSFICVHFVLMA-LLLAVVFDAYSEEHRSHIVSSLVKEKRNLMLAFHCIDRDNSGFVDVSEWEDLLRVIRPDLDPEQ-----------SRLMFALLDTDGDGELNVDEFTMLADMLTWKF--------------------ERLPERDLWQRMWDKVASVIGVPLALLYLVAIGKPLEYVYKAAIIVNAILHLVYVEDTLPNFVAYLLLIFVIFFTLVLAIKLCGLGYSTFKSSAFNLWDTFVIGTTWILYICYKAGWVPH--------FYNVTCFLLLSLWNVRQRHNGLIAKMLGIDERFDSFLASVKQSGSIIFSLLVVVCLLLYSYAIIGMELFAGRFDKPANPPYDNEE-------ANFNDFGRALLTCFQVLATNNFNEIARYAAYSTGNPYTSWYFISL 722          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|1187613735|ref|XP_020759835.1| (two pore calcium channel protein 1 isoform X2 [Odocoileus virginianus texanus])

HSP 1 Score: 112.464 bits (280), Expect = 1.806e-21
Identity = 147/604 (24.34%), Postives = 263/604 (43.54%), Query Frame = 0

Query:  568 LELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLI-C-LSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRR-GWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTL---ELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRK-FSSAFRVLPFFLSSFSLCVLI-SYSWAVLGMALFAKFGDEIPTAIHQELV-------------------GWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFL 1144
            LEL  +++   +LC+++  LG+  F       R +  T                 ++++ F +    L       R +R LR ++L+ C     ++R    + + L    D+ +    F+ +F++LG  LFS       S PY  F    NS ++L+VL+TT NF  ++ P+Y+ NP         FF+ ++ I + F+ +L L  VF  + + ++R+     + +R AI   +R L  QRR   I    F  ++R+ +P              AG     F+ L +S+   +  ++F  + EV    +        +   +   W          I     V V  + +L+ T F L   G   +SK       +S +V  T+   EL LK+   GP  ++S   N+ D+ +   +F+ LL  ALA    P +         ++   L+++RLF     +R    + F +LP  ++S  L +LI  YS+A++GM  F   G   P   +   V                   G+  ++NF++  ++ VTLF++    +WY IM      T + W+ +YF+ F++V  +VV  +  A  ++AF+
Sbjct:  144 LELFALVVVVFELCMKLRWLGLHTFIRHR---RTMVKTS----------------VLVVQFVEAIVVLVRQTSHMRVTRALRCIFLVDCRYCGGVRRNLRQMFQSLPPFMDILMLLLFFMIIFAILGFYLFSPN----PSDPY--FSTLENSIISLFVLLTTANFPDVMMPSYSQNPWSC-----IFFIVYLSIELYFIMNLLLAVVFDTFNDVEKRKFKSLLLHKRTAIQHAYRLLVSQRRSAGISYRQFEGLMRFYKP-----------RMSAGERYLTFKALNQSNTPLLSLKDFHDIYEVAALKW--------KAKRNREHWFDELPRTAFLIFKDLVVAVNGVWMLVET-FMLK--GGNFVSK----HVPWSHLVFLTIYAVELFLKVAGLGPIKYLSSGWNLFDVCVTAFAFLGLL--ALAFNTEPFY-------FIVVLRPLQLLRLFKLKKRYRNVLDTMFELLP-RMASLGLTLLIFYYSFAIVGMEFFC--GILYPNCCNTSTVADAYRWLNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQT-SHWSRLYFMTFYIV-TMVVMTIIVAFILEAFV 677          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|573905623|ref|XP_006640532.1| (PREDICTED: two pore calcium channel protein 1 [Lepisosteus oculatus] >gi|973204175|ref|XP_015221885.1| PREDICTED: two pore calcium channel protein 1 [Lepisosteus oculatus])

HSP 1 Score: 112.079 bits (279), Expect = 2.564e-21
Identity = 152/612 (24.84%), Postives = 262/612 (42.81%), Query Frame = 0

Query:  568 LELVLVLLFSLDLCLEIYALGVGAFFGMNAPPRVLENTGSRSGLQAKLWGQFRLFLVILFFADICAALDSHNESPRFSRPLRPLYLI-C-LSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCL-DPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVI------------LTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRK-FSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELV-------------------GWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFL 1144
            LEL+ +L+ + +LC+++  LG   F       R +  T                 +++L F +    L       R +R LRP++L+ C     ++R    + + L    D+ L    F+ +FS+LG  LFS+      S PY  F+   NS ++L+VL+TT NF  ++ PAY+ N          FF+ ++ I + F+ +L L  VF  +   ++ +     + +R AI   F+ L   QR   +    F  ++R+ RP          +D F       F+ L R     +  ++F +  EVI              D  P  P L   G++          + +A   + +  V    + IL   F+   G  S+SKL    + + + + + +E++LKI   GP  + S   N+ D  +   +F+ LLV  LA    P +         ++   L+++RLF     +R    + F + P   S     ++  YS+A++GM  FA  G   P       V                   G+  ++NFN+  S+ VTLF++    +WY  M      T T W+ +YF+ F++V  +VV  +  A  +DAF+
Sbjct:  144 LELLALLMVAFELCMKLRWLGFQTFI---RHKRTMVKTT----------------VLLLQFVEAIVVLVRQTSHMRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFSILGFYLFSQN----TSDPY--FNTLENSIVSLFVLLTTANFPDVMMPAYSRNRWSC-----IFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIDHAFQLLVSRQRPNGVSFKQFDGLMRFYRPRM------NARDRFLT-----FKALNRCGSPLLSLKDFYNFYEVIGLKWKARRSGEHWFDDLPHTPFLIFKGINI-------LVKSKAFQYAMYAVVAVNGIWILVDTFMLNSGF-SLSKLVPWSYIFFLTI-YGVEMLLKISGLGPLEYFSSGWNLFDFSVTVFAFLGLLV--LAFDMEPFY-------FIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFA--GVVYPNCCVNSTVADSYRQVSITVGNRTVLDEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQT-THWSRLYFMTFYIVT-MVVMTIIVAFILDAFV 692          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|167516930|ref|XP_001742806.1| (hypothetical protein [Monosiga brevicollis MX1] >gi|163779430|gb|EDQ93044.1| predicted protein [Monosiga brevicollis MX1])

HSP 1 Score: 109.768 bits (273), Expect = 8.613e-21
Identity = 137/529 (25.90%), Postives = 244/529 (46.12%), Query Frame = 0

Query:  620 RLFLVILFFADICAALDSHNESPRFSRPLRPLYLIC--LSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFS----ETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRE--------RARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSY--------SIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRK-FSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHL 1125
            R  +++  FA+    L  + +  R  R LRP +LI       ++R S  +++ +  + D+      F+ + +++G  LFS    + G+   S           +F++L++L+TT N+  +  PA ++N       YF FF+ +++I +  L ++ L  V+  +++ +R +  +  +  R AI   +R L   R G I + SFR ++++ +  F  LQ            + MF  L +S  G +  RE        L ++Y     + +   +  E H R         R+R R  +       G M  +IL    L FY +  +S    R+  Y          +V+F LE+V+K    GP  ++    N LDLL+   S I LL+     P+S         N+ L    +R++RLF     FR+   + F ++P   S   + VL+ YS+A++G+ALFA  G       +   V    ++NF+  ++A VTLF++    +W+ IM  ++ +T T WA  YF+ F++
Sbjct:  121 RTLILVGLFAESLVVLFRNKDHFRVLRLLRPYFLIDNPWMANVRRVSRQIVQSMPPIIDMLFLLLFFMSMMAIMGYYLFSSNDQDDGFSTLS----------AAFVSLFILVTTANYPDVQMPALSSNRW-----YFVFFLVYLIIGLYCLLNILLAVVYDEFRSQERIKFQKLYLHRRAAIRHAYRLLKDDRYGGIPLDSFRGLMKHRKRRFTPLQ-----------VKLMFLALNQSESGAINLRE--------LYNFYMYETLVWKQIKNDDEHHGRYNPMTFRQIRSRIRDAVQHPIFNYG-MGFIILLNTCLVFYYAGIVSAEDARQSRYPETQTIDTIFLVVFWLEIVIKFVGLGPWDYLRDNWNRLDLLVTTLSTIGLLL-----PNS--------TNIFLSLRQIRMLRLFKVKRRFREVLGTVFSLVPMLSSYIVVLVLVYYSFAIMGIALFAGRGCCGDYYSNATAVDRYYLNNFDDIFNAYVTLFELMIVNNWFVIMNGFVAVTNT-WAHAYFMLFYI 600          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|1022770066|gb|KZS14480.1| (Two pore calcium channel protein 1 [Daphnia magna])

HSP 1 Score: 104.375 bits (259), Expect = 5.702e-19
Identity = 148/642 (23.05%), Postives = 271/642 (42.21%), Query Frame = 0

Query:  616 WGQFRLFL-----------VILFFADICAALDSHNESPRFSRPLRPLYLICLSET--LKRWSSLLLRLLGQLRDVALGTGIFLGLF-SLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFR-CLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERARFRAILGSR------WVEVGSMALLILT---CFFLTFYGSPSIS-----KLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVL----RVVRLFPNCTIFRK-FSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPTAIHQELV-------------GWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDKRWVEPKEEEENTEAPALTEGLGNSLPSRNGKEEERRFDAPPGAGASYDGEDSYEGGQSWHQ 1210
            W  F++F+           +++  A+    L   +   R +R LRP++L+       ++R+   +L+ +    D+ LG  +F+ LF S+LG  +F        + PY  F     +F++L++L+TT NF  ++ P+Y      S      FF+ ++ IV+ FL +L L  V+  + + +R++  +  +  R A    FR  L    RG I +  FR +LR V P     +T  E          MF  L  S  G +   EF  + +V+   +        R       W  R R+R  A +  +      W        L++       LT   + S+      +L +   +Y+ V  + LE  LK++  G  ++ S   N  D  + G S I L    L                   FLV+    R++R+F     +R  F + F +    L +  + +++ Y+++++GM LFA++  ++        V             G+  ++NF +  S+ VTLF++    +WY IM  +  +T + W+ +YF+ F+L   I++  + A++ +D F+ F     +  KE+    +   L E       + N +E ER          + +  +    G+ WH 
Sbjct:  165 WVGFKIFIHHPRSMVKALTLLIMIAESIVVLVRQSSHFRVTRALRPIFLLDNHHFGGVRRYLRQVLQSVPPAIDM-LGLLLFVILFYSVLGFYIFGSN----VNDPY--FSTLQQAFISLFILLTTANFPDVMMPSYAVTRWSSV-----FFITYLSIVLYFLMNLLLAAVYASFSSMERKKFQQLLLHRRKAAQHAFRLLLSRSNRGGITLQHFRGLLRNVDPS----RTYREMYL-------MFRLLNTSDSGIINLNEFYHIYDVLDMKW--------RIRNEEPFWFSRLRSRTLATMAQQVHRLVTWTFFDYFVCLVIFLGGILILTRTAAISVHMSNPLELGVTWETYAFVSFYALEACLKMFGMGFHVYFSSGWNCFDFAVTGVSLIGLAAEILGQWS---------------FLVVARHSRILRVFKLRKRYRDVFGTIFILGQRLLCAGIVFLILYYAYSIVGMELFAEY--DLTNCCKNSTVEANFRFENGSSSNGYYYLNNFENVISSYVTLFELTVINNWYIIMEGYA-VTTSQWSRIYFMCFYLTIMILLSIVVASV-LDGFM-FR----ISYKEQMTKDDEKRLVE----KTVTLNAEEYERLL--------AVESAEQLSKGKLWHN 739          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|1159624783|gb|OPJ58956.1| (two pore calcium channel protein 1-like [Patagioenas fasciata monilis])

HSP 1 Score: 102.449 bits (254), Expect = 2.417e-18
Identity = 140/588 (23.81%), Postives = 260/588 (44.22%), Query Frame = 0

Query:  641 SPRFSRPLRPLYLICLSET--LKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAAS-IPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYESRILERLAILMTFRCLDPQRRGWIDV--TSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTLDRPGMSASEWHQRERA----RF-RAILGSR-WVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPA--NVLDLLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKF-SSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKFGDEIPT-------------AIHQELVGWASV--DNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDKRWVEPKEEEENTEAPALTEGLGNSLPSRNGKEEERRFDAPPGAGASYDGE 1199
            S R+SR +RP++LI  +E+  ++R    +   L ++  V L     L +FSL+ L LF E     A  +PY  F ++L     LYVL+TT N   ++ PA+       S  Y  FF+AFV++       L L  V+  Y+   + +  E   ++R  +L  F  L  +      V    ++ +++ V PD     +   ++     ++           G V  + F+ + +++       V TL       ++W  R  A    RF R+++  R +V    + +LI   F      SP IS        +  + L+ +E++LK++ Y P+ F       N  D LI+ A+  +++++ +    +  + S+QI ++  +  VLR++R+  +   FR   ++   ++P  L+   L +++   +A++GM LF       P              A+   L   A    +NFN+F S+ + L ++     W+     + ++T  P A +YF+AFH+V  I++  +  +  ++AF   +    +E  E E   E      G+G      +   +E+  D   GA    +GE
Sbjct:  135 SIRWSRIVRPIFLINFAESRQIRRAFRSIRNTLPEITYVFLLFMFSLLMFSLMALKLFGERNLQTAEGLPY--FRNYLEIVFDLYVLVTTANSPDVMMPAF-----DFSSWYALFFIAFVIVNTYIFMSLFLAVVYNNYKKHLKNEIRELAYMKRRKMLEAFNLLKEEEAAQFVVREARWKQLVKLVAPDI----SNSHRELLLRISDD-------EEKGFVDKKSFVQLADLLNIQ----VVTLKIRSHPLAQWVPRVYASGVSRFLRSMVRHRGFVWTYDVIILINAVFIALDEESPYISYAE-----WVFLALYIIEILLKVYTYEPRAFFGKNQFWNWFDTLIIFAALTAMILN-ITLKSTVKYNSQQILDIVFILRVLRLIRIVDSIQRFRVIMNTLINIVPTMLTFGGLTLVVYCVFAIVGMELFHGKIQFFPANSNAPHALECGNPALKDSLFARAKYCKNNFNNFASSFIVLMELTVVNQWHVFANGFANVTAQP-AKLYFIAFHIVMVIIIVNIFVSFILEAFFVEYS---LEKSEVETAIERKIQELGMG---VQEDELRDEQLLDNMEGAEHDLEGE 687          

BLAST of EWM28530.1 vs. NCBI_GenBank
Match: gi|168027922|ref|XP_001766478.1| (predicted protein [Physcomitrella patens] >gi|162682387|gb|EDQ68806.1| predicted protein [Physcomitrella patens])

HSP 1 Score: 102.064 bits (253), Expect = 2.982e-18
Identity = 151/593 (25.46%), Postives = 244/593 (41.15%), Query Frame = 0

Query:  608 RSGLQAKLW-GQFRLFLVILFFADICAALDSHN------ESPRFSRPLRPLYLICLSETLKRWSSLLLRLLGQLRDVALGTGIFLGLFSLLGLILFSETGYYAASIPYLNFDDFLNSFLALYVLMTTQNFGALLNPAYTANPQGSSPLYFFFFMAFVLIVVLFLAHLALPRVFWVYQNFQRRQAYE--SRILERLAIL-MTFRCLDPQRRGWIDVTSFRSMLRYVRPDFFDLQTGYEKDAFAGATEHMFEELARSHPGRVYPREFLSVCEVILTDYYPCVPTL--DRPGMSASEWHQRERARFRAILGSRWVEVGSMALLILTCFFLTFYGSPSISKLSLRRFSYSIVVLFTLELVLKIWAYGPKLFVSLPANVLD----LLIVGASFISLLVHALAPPHSPLHQSEQIANLCLLFLVLRVVRLFPNCTIFRKFSSAFRVLPFFLSSFSLCVLISYSWAVLGMALFAKF---GDEIPTAIHQELVGWASVDNFNSFWSASVTLFQVATTYDWYRIMYLWMHLTRTPWAAMYFLAFHLVANIVVYQLTAALFIDAFLSFHDK----RWVEPKEEEENTEAPALTEGLGNSLPSR 1177
            R  L +K W  Q  ++ + L  A +C ++ + +      +  + S  LR L  I  S  ++      + ++    D+     IF+ L + L    FSE+         L F D+  S L L+VL+TT N     NP+  +    ++ L FFFF  ++L+   FL +LA   +   Y N++ + A E   R   R   L   F  LD + + WID  +  ++       F  +            T H+F  L R    +++  EF  +C+VI  +     P L   R  MS +E        +  +  +  + +GS+A  I     L   GS  +  LS+  F +  V  F ++ +LK++  G K + S   N  D    LLI+   FIS       P  + L     +    L+ L+ R  RL     I    ++A    P     F +C + S    VLGM LF      G+          +G+ +  N+N + SA  T F +     WY IM  +   T + W+ +YF+ F  +A +    +  A F +A     +K    R  E +  +E T AP      G +LP R
Sbjct:  255 RYFLGSKFWQNQHSVYKMALLLA-MCISISASSLGIAEYQDIKISMYLRILIPITFSRAIRGCFRRTVLIVHTFMDITGLVVIFVMLSAWLATTFFSEST--------LEFKDYGTSLLNLFVLLTTAN-----NPSVWSTAYRTNRLAFFFFSTYLLVGFFFLMNLAFSVI---YSNYKAQMAVEVAKRTTARQGNLKAAFSLLDVRHQEWIDGATMIAL-------FLAIGRYRHISDIRARTSHLFLALNRRGDFKIWSDEFEELCDVIAKEV-ESPPDLFQSRRRMSKNEMQFVTHPVYTYVTWA--LTLGSLAAAITQ---LNVGGSAQVLLLSIE-FLFGWV--FAMDALLKVYLQGWKSYWSSTLNAFDFVVTLLILALHFISFFNENARPWVTYLLLVRGLRTFALVSLITRW-RLMVQTLIIVIPATA----PILALQFLVCSMFS----VLGMHLFGGLVYEGNYALAGTEYLTLGFEAF-NYNDYASAMATSFNLCVVNKWYVIMDGYAAATNSRWSRIYFMTFWAIAVVFTLNVVVAFFTEALTCQMEKAERIRAREMRRNQEETSAPVRRRS-GLNLPFR 803