EWM28746.1, cds1954 (CDS) Nannochloropsis gaditana

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|585111169|gb|EWM28746.1| (dna topoisomerase 6 subunit b [Nannochloropsis gaditana])

HSP 1 Score: 1520.37 bits (3935), Expect = 0.000e+0
Identity = 729/729 (100.00%), Postives = 729/729 (100.00%), Query Frame = 0

Query:    1 MARKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHKATGKRRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRKRIVNSENWTRSNTDGFSQPQSARHKQAKLFDFIERKWDTNPHTFMSSHLNLVEEARNMDLVDKKLQNRLKSAIDRVDLESALEVAAINRSNGGLISKERLFVAPLQLKTSLTTSLLSPLHSVHFYSSALVAICTPHNEKSHKTTSSIEVPK 729
            MARKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHKATGKRRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRKRIVNSENWTRSNTDGFSQPQSARHKQAKLFDFIERKWDTNPHTFMSSHLNLVEEARNMDLVDKKLQNRLKSAIDRVDLESALEVAAINRSNGGLISKERLFVAPLQLKTSLTTSLLSPLHSVHFYSSALVAICTPHNEKSHKTTSSIEVPK
Sbjct:    1 MARKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHKATGKRRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRKRIVNSENWTRSNTDGFSQPQSARHKQAKLFDFIERKWDTNPHTFMSSHLNLVEEARNMDLVDKKLQNRLKSAIDRVDLESALEVAAINRSNGGLISKERLFVAPLQLKTSLTTSLLSPLHSVHFYSSALVAICTPHNEKSHKTTSSIEVPK 729          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|1072232153|gb|OEU16885.1| (topo-VIb_trans-domain-containing protein [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0
Identity = 332/688 (48.26%), Postives = 454/688 (65.99%), Query Frame = 0

Query:    4 KNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHKATGK-RRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNK-KKGDRGAR------NYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRKRIVNSENWTRSNTDGFSQPQSARHKQAKLFDFIERKWDTNPHTFMSSHLNLVEEARNMDLVDKKLQNRLKSAIDRVDLESALEVAAINRSNGGLISKERLFVAPL 683
            + R E+   K+PAEFF+++Q+I GFDN G+SLYT++RELVEN+LDA ESI  LP + V IEE+ +DE N++RG+    + D+ LF +  GK   S G +            S A +K+ G N +  ++K  K  +  R       Y+++  +DNGCGM HEKIPD+LGRVLS SKYGVRQTRGKFGLG+KMALIWSKKSTG+PI++ +AHS         +S CVLDI+I  N P +  H K  N   W GT +++++ GNW+TY++ I++YFQQLAIITPY  +E  +   S   R +  ++ RRSE MPP  +E+KHHPSS+N++I+ QLI+R++ KT ++FL  EL+CVTRPLA RIV E G    + + P D+ + +  +L  +L+++ +FK PDGSCLSP GEYNL LGI K L P++VAT  ++ +A+EGHPF+VEA VSLGG   KDG+NV RFANRIPLLFE G DV T+VAN +I W  YKID KRDRIGVFVSIVSTKIP+KGTGKEYIGDD   I  SVK A+Q CC+QL+ HLL RNA R+ K+R+  L++Y+PDVSR++  +L+ + KR+          S  D  S P+  R +Q       E++W       + S L L+E+    ++    L+ RL+ A+D  +L    E A  + S   +     L++ P+
Sbjct:   12 EGRGEIQRQKSPAEFFADNQAIAGFDNYGKSLYTSIRELVENSLDACESIGVLPTIQVYIEELNQDEFNKIRGISVATK-DVELFQQKGGKGNSSAGAV------------SSAKKKKAGVNGSGGNDKDSKPTKTPRKKASQEGYFQLKVKDNGCGMNHEKIPDLLGRVLSGSKYGVRQTRGKFGLGAKMALIWSKKSTGVPIKITTAHS---------VSICVLDINITNNCPNIIQHTKSKNTTEWVGTELELLIAGNWTTYRSRIVSYFQQLAIITPYCQLELVYENRSEPQRNMTLRHDRRSEQMPPLPREIKHHPSSVNNIIVSQLIERSNCKTIIDFLTKELSCVTRPLAQRIVTECGWDEDDIVNPSDMTSRQLTKLVQVLRTVKMFKAPDGSCLSPLGEYNLNLGIRKVLEPEFVATARDKPSAYEGHPFLVEAAVSLGGKEAKDGLNVVRFANRIPLLFEGGADVCTRVANNKIKWSLYKIDQKRDRIGVFVSIVSTKIPYKGTGKEYIGDDITEISVSVKKAIQSCCQQLRVHLLKRNALRNQKDRRSKLVKYIPDVSRSLYTLLDGMRKRR------EEHPSGDDPMSSPRK-RPRQ-------EQQWQGQ-RKHVDSVLGLLEKG---EITASVLKKRLEEAVDVQNLVEEEEEAKKSHSKEAI----PLYIKPI 655          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|223999337|ref|XP_002289341.1| (topoisomerase [Thalassiosira pseudonana CCMP1335] >gi|220974549|gb|EED92878.1| topoisomerase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 626.32 bits (1614), Expect = 0.000e+0
Identity = 307/579 (53.02%), Postives = 407/579 (70.29%), Query Frame = 0

Query:    8 EVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMES-------RERVDLGLFHKATGKRRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSIS-IFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRK 578
            E+ +SK+PAEFF+E+QSI GFDNPG+SLYTT+RELVEN+LDA ES+  LP +SVVIEE+T+D+ NE+RG+ +       ++ VD G    A G   SG +      +N K ++    +K+                G   Y+ VT  DNGCGMAH+ IPD+LGRVLS SKYGVRQTRGKFGLG+KMALIWSKKSTG+PI ++++H         YI      IDIY+N P +  H++  N + W GT + V+V GNW+TYK+ ++ Y QQLAIITPYA +E ++   S+  + +  ++ RRSE MP  A+EVKHHPSS+N+L++QQL++ + +KT L FL  +L+ ++  +A R+++ LG+ F E+M P ++D  +  RL  +L+S   +FK PDG CLSP GEYNL LGI K + P+ +AT  ++  A++GHPF+VEA VSLGG   K+GI V RFANRIPLLFE G DV T+VAN +I W SYKID KRD+IGVFVSIVSTK+PFKGTGKEYIGDD   I+ SVK ALQ CC+QL+ HL  RNA RDVKERK  LL+Y+PDVSR++  +L  ++KRK
Sbjct:   12 EIQQSKSPAEFFAENQSIAGFDNPGKSLYTTLRELVENSLDACESVDVLPDISVVIEEMTQDQFNEMRGVPTSGGPGKKKKGVD-GSDDAAVGDAESGDK------DNGKGVNEKGAKKRRA--------------GGDAYFLVTVRDNGCGMAHDAIPDLLGRVLSGSKYGVRQTRGKFGLGAKMALIWSKKSTGVPIRIKTSHV-----KGIYI------IDIYKNCPRIIEHKQKDNTENWIGTEMQVLVSGNWTTYKSRVVQYLQQLAIITPYARLEMSYSNRSDEKKGMNLRFERRSEQMPSQAREVKHHPSSVNNLLVQQLLENSKSKTLLKFLTGDLSGISPSVAKRLIERLGDAFEEDMSPAEMDDKQITRLVQLLRSTDDLFKSPDGGCLSPLGEYNLNLGIQKVVEPEIIATARDKPGAYDGHPFIVEAAVSLGGKEAKEGITVVRFANRIPLLFEGGADVATRVANSKIKWSSYKIDHKRDKIGVFVSIVSTKVPFKGTGKEYIGDDITEIQLSVKRALQNCCQQLRVHLAKRNALRDVKERKSRLLKYIPDVSRSLFGILEGMHKRK 558          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|729353427|ref|XP_010544238.1| (PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Tarenaya hassleriana])

HSP 1 Score: 625.55 bits (1612), Expect = 0.000e+0
Identity = 311/574 (54.18%), Postives = 412/574 (71.78%), Query Frame = 0

Query:   10 VESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFH-----KATGKRRSG-GEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVIT-QYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINK 576
            ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI +LP V + IEEI + + N + G+  RERVD  L+      KA  KR +     QE+ A+N       A  K++ +   A+S   KG RG  +YY+VTC+DNG GM H+ IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S+   +     +Y++ C LDIDI+RN P V LHEK  N + W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA  +F FR +S+   + IT ++ARR++ MPP   E KHHPSS++ L+I++LI  TS KT L FL  E   + + LAAR++ E+G  F+ NM  + + + + +R+  + +    F  P G CLSPAGEYNLRLG++KELHPD VAT S  A  FEGHPF+VEAGVSLGG  +K GIN++RFANRIPLLFE G DVVT+ A +RINW +YKI+  +D+IGVF+SIVSTKIPFKGTGKEYIGDD   I  +VKSA+Q+CC QLK  ++ R  AR+ +ERKR+L RY+PDVS A+  VL  +++
Sbjct:   26 LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISQLPEVEITIEEIGKSKFNSMIGLVDRERVDEKLYDDYETVKAREKRLAKEARAQEIQAKNA------ASGKKVKE--PAVSKVLKG-RGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQ-----SYVTFCRLDIDIHRNIPHVHLHEKKGNTERWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYA--QFLFRFISDTPEKNITIKFARRTDVMPPVPVETKHHPSSVDLLLIKRLIADTSKKTLLQFLQNEFVNINKSLAARLIGEMGPDFSPNMAVKSLTSQQMVRIHQLFRQAK-FDDPSGDCLSPAGEYNLRLGVIKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGKDVKQGINIFRFANRIPLLFEQGGDVVTRTALKRINWSTYKINQTQDKIGVFISIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCNQLKSKIVKRIQAREQQERKRNLSRYIPDVSGAVYDVLKEMSR 582          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|219125348|ref|XP_002182945.1| (type II DNA topoisomerase 6 subunit [Phaeodactylum tricornutum CCAP 1055/1] >gi|217405739|gb|EEC45681.1| type II DNA topoisomerase 6 subunit [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 625.165 bits (1611), Expect = 0.000e+0
Identity = 302/573 (52.71%), Postives = 403/573 (70.33%), Query Frame = 0

Query:    8 EVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMES--RERVDLGLFHKATGKRRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNASREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRK 578
            EV   K+PAEFF+E+Q+I GFDN G+SLYT++RELVEN+LDA ESI ELP +S+ I+E T++E N L  M++  R++ D+ LF      ++   E             +D  E    +NS     +KK  + A  Y+++T +DNGCGM+H  IP++LGRVLS SKYGVRQTRGKFGLG+KMALIW+KKSTG PI + ++H  +        S CVLDIDIY+N P +  H    N  GW GT + V++ GNW+TYK+ I+ Y QQLAIITPYA +E  +  +S++ R +  +Y RRS+ MPPPA+ +KHHP+S+N+L+IQQLI+ T T+T   FL TEL+ V+ PLA R+V EL  GF E+M P  ++  +  RL  +L+ + +FK PDGSCLSP GEYNL LGI K L PD +AT  ++  A+EGHPF+VEA VSLGG  +K+GI V RFANRIPLLFE G DV T+VAN +I W +YK+D KRDRIGVFVSIVSTK+PFKGT KEYIGDD   I++SVK ALQ CC+QL+ +L  R+A +D + RK  + +Y+PDV R++  +L+S+  R+
Sbjct:   15 EVQVQKSPAEFFAENQAIAGFDNLGKSLYTSLRELVENSLDACESIHELPEISIEIKEYTQEEFNALDVMKTSPRKKRDMQLFETKKKDKKPPKE------------ATDVDEITTVENSG--KKRKKAPQDA--YFKLTVKDNGCGMSHSAIPNLLGRVLSGSKYGVRQTRGKFGLGAKMALIWAKKSTGQPIRIMTSHRPDGGVAPERASACVLDIDIYKNAPRILEHTTRKNTDGWMGTEMSVLIAGNWTTYKSRIVQYLQQLAIITPYAVLELAYINISDSKRNLHVRYDRRSDQMPPPAKTIKHHPASVNNLVIQQLIQFTKTRTLFKFLSTELSTVSPPLARRLVTEL--GFDESMPPSSLEDKEITRLVQLLRQVQLFKAPDGSCLSPLGEYNLNLGIRKVLEPDLIATSRDKPGAYEGHPFLVEAAVSLGGKEVKEGITVIRFANRIPLLFEGGADVATRVANTKIRWSNYKMDYKRDRIGVFVSIVSTKVPFKGTSKEYIGDDATEIQQSVKRALQSCCQQLRGYLAKRSALKDAQTRKSRMAKYVPDVGRSLFGILDSMRARQ 569          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|1072959506|ref|XP_018490667.1| (PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Raphanus sativus])

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 310/587 (52.81%), Postives = 410/587 (69.85%), Query Frame = 0

Query:    2 ARKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHK-ATGKRRSGGEIQEVLAENM--KELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNA-SREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSI-----NKRKR 579
            +++++   ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD++ESI ELP V V IEEI + + N + G+  RERVD  L+    T K R    ++E  A  +  K L S    K+ G +    +      RG  +YY+VTC+DNG GM H+ IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S+   +      Y++ C LDIDI+RN P + LHEK AN + W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA  +F FR +S    + V  ++ RR++ MPP   E KHHPSS++ L+I++L+  TS KT L FL  E   + + LAAR++ E+G  F+  M  + + A + +R+  + +    F  P G CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPF+VEAGVS+GG  +K G+N++RFANRIPLLFE G DVVT+ A +RINW SYKI+  +D+IGVFVSIVSTKIPFKGTGKEYIGDD   I  +VKSA+Q+CC QLK  ++ R  AR+ +ERKRSL RY+PD + A+  VL  +     NKRKR
Sbjct:   21 SKESKESTLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSSESISELPEVEVTIEEIGKSKFNSMIGLIDRERVDTQLYDDYETDKAREKRLVKEARASEIQAKNLASGKKNKEPGVSKVLKA------RGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQN-----YVTFCRLDIDIHRNIPHIHLHEKKANKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYA--QFLFRFISETPEKNVTVKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLVTDTSKKTLLQFLQNEFVNINKNLAARLIGEMGPDFSPGMAVKSVTAQQMVRIHQLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATHSGSAQVFEGHPFIVEAGVSVGGRDVKQGMNIFRFANRIPLLFEQGGDVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVKRLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHTNKRKR 593          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|357454399|ref|XP_003597480.1| (DNA topoisomerase VI, B subunit [Medicago truncatula] >gi|355486528|gb|AES67731.1| DNA topoisomerase VI, B subunit [Medicago truncatula])

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 309/574 (53.83%), Postives = 407/574 (70.91%), Query Frame = 0

Query:    3 RKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHK-ATGKRRSGGEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNA-SREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSI 574
            RK +  V++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI ELP V + IEEI + + N + G+  RERVD  L+    T K R     +E  A+ ++  ++ AL K++    A  S   KG RG  ++YRVTC+DNG GM H+ IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S+   +      YIS C LDIDI++N P V LHEK  N + W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA  +F F  +S+A  + V  ++ARR++ MPP   E KHHPSS++ L+I++LI  TS +  L FL  E   +++P A R++ E+G  F+  M  + + + + +R+  + +    F  P G CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPF+VEAGVS+GG  +K G+N++RFANRIPLLFE G DVVT+ A +RINW SYKI+  +D+IGVFVSIVSTKIPFKGTGKEYIGDD   I  +VKSA+Q+CC QLK  ++ R  AR+ +ERKR+L RY+PD S A   VL  +
Sbjct:   20 RKPKETVLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPVVEITIEEIIKSKFNSMIGLVDRERVDAALYDDFETAKAREKRLAKEARAQELQAKNA-ALGKKVKDTPA--SKGMKG-RGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKKQN-----YISFCRLDIDIHKNIPHVHLHEKRENNQNWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFQFKF--VSDAPDKNVTVRFARRTDVMPPIPLETKHHPSSVDLLLIKRLITETSKQNLLQFLQHEFVNISKPHAERLIGEMGPDFSSKMAVKSLTSQQLVRIHQLFRQAK-FDDPSGHCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSVGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWSSYKINQVQDKIGVFVSIVSTKIPFKGTGKEYIGDDITEIASAVKSAIQQCCIQLKSKIMKRIQAREQQERKRNLSRYIPDASGAFYNVLKQM 581          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|30685928|ref|NP_188714.2| (topoisomerase 6 subunit B [Arabidopsis thaliana] >gi|75169001|sp|Q9C5V6.1|TOP6B_ARATH RecName: Full=DNA topoisomerase 6 subunit B; Short=AtTOP6B; AltName: Full=Protein BRASSINOSTEROID INSENSITIVE 3; AltName: Full=Protein ELONGATED HYPOCOTYL 6; AltName: Full=Protein ROOT HAIRLESS 3 >gi|12331188|emb|CAC24690.1| topoisomerase 6 subunit B [Arabidopsis thaliana] >gi|332642901|gb|AEE76422.1| topoisomerase 6 subunit B [Arabidopsis thaliana])

HSP 1 Score: 622.083 bits (1603), Expect = 0.000e+0
Identity = 313/582 (53.78%), Postives = 405/582 (69.59%), Query Frame = 0

Query:   10 VESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLF-----HKATGKRRSG-GEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNA-SREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSI-----NKRKR 579
            ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI ELP V V IEEI + + N + G+  RERVD  L+      KA GKR +      E+ A+N   L S    K+ G +    +      RG  +YY+VTC+DNG GM H+ IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S+   +      Y++ C LDIDI+RN P + LHEK  N + W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA  +F FR +S    + V  ++ RR++ MPP   E KHHPSS++ L+I++LI  TS KT L FL  E   + + LAAR++ E+G  F  +M  + + + + +R+  + +    F  P G CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPF+VEAGVSLGG  +K GIN++RFANRIPLLFE G DVVT+ A +RINW SYKI+  +D+IGVFVSIVSTKIPFKGTGKEYIGDD   I  +VKSA+Q+CC QLK  ++ R  AR+ +ERKRSL RY+PD + A+  VL  +      KRKR
Sbjct:   25 LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQAKN---LASGKKNKEPGVSKVLKA------RGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQN-----YVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYA--QFLFRFISETPEKNVTIKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGPDFGPSMAVKSVTSQQMVRIHQLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVKRLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKR 589          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|922479461|ref|XP_013637802.1| (PREDICTED: DNA topoisomerase 6 subunit B [Brassica oleracea var. oleracea])

HSP 1 Score: 619.387 bits (1596), Expect = 0.000e+0
Identity = 310/592 (52.36%), Postives = 409/592 (69.09%), Query Frame = 0

Query:    2 ARKNRIEVVESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLFHK-ATGKRRSGGEIQEVLAENM--KELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNA-SREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSIN-----KRKRIVNSE 584
            ++ ++   ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD++ESI ELP V V IEEI + + N + G+  RERVD  L+    T K R    ++E  A  +  K L S    K+ G +    +      RG  +YY+VTC+DNG GM H+ IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S+   +      Y++ C LDIDI+RN P + LHEK AN + W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA  +F FR +S    + V  ++ RR++ MPP   E KHHPSS++ L+I++L+  TS KT L FL  E   + + LAAR++ E+G  F+  M  + + + + +R+  + +    F  P G CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPF+VEAGVS+GG  +K GIN++RFANRIPLLFE G DVVT+ A +RINW SYKI+  +D+IGVFVSIVSTKIPFKGTGKEYIGDD   I  +VKSA+Q+CC QLK  ++ R  AR+ +ERKRSL RY+PD + A+  VL  +      KRKR    E
Sbjct:   18 SKNSKESTLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSSESISELPEVEVTIEEIGKSKFNSMIGLIDRERVDTKLYDDYETDKAREKRLVKEARASEIQAKNLASGKKNKEPGVSKVLKA------RGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQN-----YVTFCRLDIDIHRNIPHIHLHEKKANKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYA--QFLFRFISETPEKNVTVKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLVTDTSKKTLLQFLQNEFVNINKKLAARLIGEMGPDFSPGMAVKSVTSQQMVRIHQLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATHSGSAQVFEGHPFIVEAGVSVGGRDVKQGINIFRFANRIPLLFEQGGDVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVKRLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHTKKRKRYKEEE 595          

BLAST of EWM28746.1 vs. NCBI_GenBank
Match: gi|727415190|ref|XP_010509333.1| (PREDICTED: DNA topoisomerase 6 subunit B-like [Camelina sativa])

HSP 1 Score: 619.387 bits (1596), Expect = 0.000e+0
Identity = 306/577 (53.03%), Postives = 405/577 (70.19%), Query Frame = 0

Query:   10 VESKTPAEFFSEHQSICGFDNPGRSLYTTVRELVENALDAAESIQELPYVSVVIEEITEDELNELRGMESRERVDLGLF-----HKATGKRRSG-GEIQEVLAENMKELHSDALEKQMGQNSAALSNKKKGDRGARNYYRVTCEDNGCGMAHEKIPDMLGRVLSSSKYGVRQTRGKFGLGSKMALIWSKKSTGLPIEVRSAHSLEENHPAAYISHCVLDIDIYRNQPFVKLHEKVANIKGWRGTRIDVVVEGNWSTYKTHIMTYFQQLAIITPYAHMEFTFRTLSNA-SREVITQYARRSEHMPPPAQEVKHHPSSINHLIIQQLIKRTSTKTFLNFLCTELACVTRPLAARIVKELGNGFAENMVPEDIDANKTIRLTSMLKSISIFKLPDGSCLSPAGEYNLRLGIMKELHPDYVATVSEQAAAFEGHPFVVEAGVSLGGNALKDGINVYRFANRIPLLFEAGNDVVTQVANRRINWQSYKIDPKRDRIGVFVSIVSTKIPFKGTGKEYIGDDTEAIRKSVKSALQRCCKQLKQHLLTRNAARDVKERKRSLLRYMPDVSRAIAAVLNSINKRKR 579
            ++ K+PAEFF+E+++I GFDNPG+SLYTTVRELVENALD+AESI ELP V V IEEI + + N + G+  RERVD  L+      KA  KR +    + E+ A+N   L S    K+ G +    +      RG  +YY+VTC+DNG GM H+ IP+M GRVLS +KYG++QTRGKFGLG+KMALIWSK STGLPIE+ S+   +      Y++ C LDIDI+RN P + LHEK  N + W G  I VV+EGNW+TY++ I+ Y +Q+A+ITPYA  +F FR +S    + VI ++ RR++ MPP   E KHHPSS++ L+I++LI  T+ KT L FL  E   + + LA R++ E+G  F+ +M  + + + + +R+  + +    F  P G+CLSPAGEYNLRLGI+KELHPD VAT S  A  FEGHPF+VEAGVS+GG  +K GIN++RFANRIPLLFE G DVVT+ A +RINW SYKI+  +D+IGVFVSIVSTKIPFKGTGKEYIGDD   I  +VKSA+Q+CC QLK  ++ R  AR+ +ERKRSL RY+PD + A+  VL  + +  +
Sbjct:   26 LKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAESISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKAREKRLAKEARVSEIQAKN---LASGKKNKEPGVSKVLKA------RGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQN-----YVTFCRLDIDIHRNIPHIHLHEKKGNKEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYA--QFLFRFISETPEKNVIIKFTRRTDVMPPIPIETKHHPSSVDLLLIKRLITDTAKKTLLQFLQNEFVNINKTLATRLIGEMGPDFSPSMAVKSVTSQQMVRIHQLFRQAK-FDDPSGNCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSVGGRDVKQGINIFRFANRIPLLFEQGGDVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCVQLKSKIVKRLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHK 585