EWM28757.1, cds1935 (CDS) Nannochloropsis gaditana

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|585111180|gb|EWM28757.1| (sphingosine kinase [Nannochloropsis gaditana])

HSP 1 Score: 1199.11 bits (3101), Expect = 0.000e+0
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0

Query:    1 MLGGWAKPCSVYVIICHTNARSALLAATRPCLESPIAFMREAREERVLLSLTLPRVLAARQAGGARRASAKFKRGLGTYGRHSFSFSHPSKGLFRPNIFTRLRQCDKYHTILLMENGDAFIDLISKKRVQLVIKGGDPCIEVGGGLQVSLYDVIGSAVIETQQSEGTKLLVYAYPRSVGRQYPCGKVYPIAKRRPQYLHFMTPKAQLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPPPRAAALPPLRARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRILGSAVLERQEK 586
            MLGGWAKPCSVYVIICHTNARSALLAATRPCLESPIAFMREAREERVLLSLTLPRVLAARQAGGARRASAKFKRGLGTYGRHSFSFSHPSKGLFRPNIFTRLRQCDKYHTILLMENGDAFIDLISKKRVQLVIKGGDPCIEVGGGLQVSLYDVIGSAVIETQQSEGTKLLVYAYPRSVGRQYPCGKVYPIAKRRPQYLHFMTPKAQLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPPPRAAALPPLRARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRILGSAVLERQEK
Sbjct:    1 MLGGWAKPCSVYVIICHTNARSALLAATRPCLESPIAFMREAREERVLLSLTLPRVLAARQAGGARRASAKFKRGLGTYGRHSFSFSHPSKGLFRPNIFTRLRQCDKYHTILLMENGDAFIDLISKKRVQLVIKGGDPCIEVGGGLQVSLYDVIGSAVIETQQSEGTKLLVYAYPRSVGRQYPCGKVYPIAKRRPQYLHFMTPKAQLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPPPRAAALPPLRARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRILGSAVLERQEK 586          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|1072224766|gb|OEU09515.1| (DAGK_cat-domain-containing protein [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 228.024 bits (580), Expect = 9.457e-64
Identity = 128/341 (37.54%), Postives = 196/341 (57.48%), Query Frame = 0

Query:  241 RQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEA-------WDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPPPRAAALPPLRARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMR 574
             + RLLV++NP +G+    + Y  ++ PMLE+AGVAH+ ++T   + A+  M    T +       +  +V +GGDG + EI+QG++ +D      L  + L ++ +GT NG +AS  +   E   P+++ F+I+K  S+   DLS  +       SFL  S+ ++AD+D+ESE IRWAG  RMD++ +  +L LR YRA+ SYLP  E    +   LPPL   L +  GWV +E DF++  +S V+H  E    +P  +M DG  QI V+R   ++SRLRL  + LA+E G HV  + VE     AYRLE +T+     LDGE +  G IQ  +LP +++
Sbjct:  194 EEERLLVIVNPCSGQKMGVSEYEKMLLPMLEEAGVAHDCLITTHTKHAEERMRKQCTPSDFKDVSKYTGIVVVGGDGTIHEIMQGIHQRDDRD-KILESIKLGMIGAGTSNGFSASLAHASKESMSPLDSAFVIVKGMSSW-IDLSRYDTKSSSYTSFLTFSWAMIADIDIESECIRWAGFLRMDIWGVVRVLFLRKYRAKFSYLPPTED--KKTVILPPLSTPLSENEGWVTVEDDFVLFWVSQVTHAGEQMFQSPSCKMNDGVFQIFVVRG--NVSRLRLALILLAMEHGGHVGMNGVEFIQCSAYRLEPITQGSYNDLDGEVIESGPIQASVLPSSIQ 528          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|223995917|ref|XP_002287632.1| (sphingosine kinase [Thalassiosira pseudonana CCMP1335] >gi|220976748|gb|EED95075.1| sphingosine kinase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 213.772 bits (543), Expect = 1.615e-58
Identity = 146/427 (34.19%), Postives = 225/427 (52.69%), Query Frame = 0

Query:  168 KLLVYAYPRSVGRQYPCGKVYPIAKRRPQYLHFMTPKAQLAKRWD-----QRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQA------VYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRM--SFLALSFGLVADVDLESEVIRWAGSFRMDLFALY-AILRLRSYRARLSYLPSKEGPPPRAAALPPLRARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRILGSAV 580
            KLLVY+YP+S   +   G+   I KR  ++  ++          D     Q I+ LA  E  L  ++    +  + +    + LV+LNP +G G   +      +Y +++ PMLEQAGV H+ +VTR+                  ++A+GGDG+L EI+QG++S+       + +L   IV  GT NG+A S L+   E   P+ ++F I K  +T P D++  + ++  +   SFL  S+GL+AD DL+SE +RW G  R D++A+Y  IL  + YRAR SYLP     P   + +P +   LP    WV +E DF++  + +VSH + + ++ P  +M DG   IL++R   S SRL+L+ + L LE GYH+   E+E+ +  AYRLE LT      LDGE +  G IQ  +LP         V
Sbjct:  134 KLLVYSYPKSSNTKKKGGEC--IGKREARHKTYVADPRCCENFSDVRSIVQAIRSLASLEHNLPIRNANIASSNNNK--PVKYLVILNPFSGGGGESSKTGARHIYETMLKPMLEQAGVEHDALVTRRGA----------------IIAMGGDGILFEIMQGVHSRLDEK-ELMQKLKFGIVGCGTSNGLAKSLLHWSGEKYGPLESIFQICKG-NTSPLDIASYQLANTTKTYTSFLTFSWGLIADCDLDSECLRWLGPIRSDIWAVYRGILCRKKYRARFSYLP-----PNTKSTVPKIEEYLPKE--WVTIEDDFIVFWVCNVSHASYNMYNCPMAKMNDGLFHILIVR--ASCSRLQLLLMLLKLETGYHIGCKELEVIDCTAYRLEPLTHNSHNDLDGELIEAGPIQAHVLPGGAHFFSGEV 529          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|1210225790|dbj|BAZ44818.1| (putative sphingosine kinase 2 [Chondrocystis sp. NIES-4102])

HSP 1 Score: 202.216 bits (513), Expect = 4.779e-55
Identity = 124/425 (29.18%), Postives = 227/425 (53.41%), Query Frame = 0

Query:  148 VSLYDVIGSAVIETQQSEGTKLLVYAYPRSVGRQYPCGKVYPIAKRRPQYLHFMTPKAQLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPPPRAAALPPLR-ARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQ 571
            ++L DV+G ++++ Q++E   L+V+AYP         G ++   +R  +  +F  P  ++  +W + I             + L G         R L +++NP +G+GQ+  ++  V+ P+L+++ + + +  T  A + K+L+  LN    D +V +GGDG + + + GL S+         ++PL ++P GTGNG+  + L   A    P++A FLI+K K     DL+ V+ ++    SFL+L++GL++DVD+ESE  ++ GS R DL+AL  I  LR+Y+ + +++P      P     P L+ AR      W  +E +F+ +   +    A   +  P  ++ DG + +L++R+    SRL L+   + L +G H++   +E Y   ++RLE LT++G+  +DGEAV    I+  ++P 
Sbjct:   40 LALKDVVGVSLVK-QKAELPGLVVHAYPIVT-----TGYIFKKQQRVLKEYYFTCPNLEVRSQWQRAIN------------NTLVGQPPDLNIPPRHLQIIINPISGKGQAPRIFQQVL-PLLDRSNLTYTVTETLSAADTKNLVAKLNLADTDGIVIVGGDGTIHDAIAGLMSRSDRDLA--IKIPLGVIPGGTGNGLCKTILELSATSYDPLDAAFLIVKGKQQ-SFDLAAVKQNNQDYYSFLSLAWGLISDVDIESEKFKFLGSLRFDLYALMLICLLRTYKGKFAFIPH-----PNCQLSPDLKTAR---QGKWHIIEDEFIFLWAMNTPWAAHDMNVAPYAQLNDGAMDVLLMRK--GTSRLELLKALMRLGKGKHLSLPHMEYYQVRSFRLEPLTKKGILVVDGEAVDYAPIEMTVIPN 432          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|299469584|emb|CBN76438.1| (Sphingosine kinase [Ectocarpus siliculosus])

HSP 1 Score: 201.83 bits (512), Expect = 1.616e-53
Identity = 143/475 (30.11%), Postives = 230/475 (48.42%), Query Frame = 0

Query:  168 KLLVYAYPRS--------VGRQYPCGKVYPIAKRRPQYLHFMTPKAQLAKRW----DQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVV-TRKAQEAKSLMH---NLNTEA--------------------WDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEG-------PPPRAAALPPLR-------------------ARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADE--VEIYNAVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRILGS 578
            +L V+AY  S         GR  PC K     KRR  +  ++      A+ W    + R+++   RE  L R           R   RRLLV++NP +G G+S++ +   + PMLEQA     +V  TR+ + A+ + +   N +T+A                     D +V +GGDG   E++QG+Y++ P     L+RL L IVP+G+GNG+A +   +  E    ++A FL  K + T   DL L E++D + ++FL + +G+++DVD+ESE  RW GS R  +  +  I  L+ YR R+S+LP  +        P   A+A  P R                   A  P   GW  +E +F++  ++  SHIA      P  R+GDG + +  +++    SR  +I +FLA+E+G H++     +  +   A+R+E LT  G  T+DGE V    +Q  +  +A  +  S
Sbjct:  135 QLQVFAYVASGEGKEMGCTGRGGPCRK----NKRRATHQTWLASDPDTAQHWATEINNRLRQSQGREEDLER---------GNRGPGRRLLVLVNPVSGTGESRSTWEKTLRPMLEQAMTTANVVFSTRRGELAEIITNAGDNSSTDATRGASAGGGGGGSTVGSLDDLDGIVVVGGDGTFFEVLQGMYAR-PDCARQLSRLSLGIVPAGSGNGLAKTVSVESGEWFGAVSASFLAAKGQ-TKAMDLLLTESADKKYLAFLNVGWGMISDVDIESEAYRWMGSLRFTVGTIVRIANLKHYRGRISFLPDLDAAAATTPTPSKNASASAPFRMPPLSEPVHQDGAGSESAAAGAPALEGWTSVEGEFILADMTQASHIAHDMPMAPHSRIGDGLIDLFFIKK--GASRFAMIQMFLAMEKGGHISPRFPCISWHRVRAFRIEPLTSSGRITVDGELVDYAPLQQHVWRKAAHVFCS 592          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|62858273|ref|NP_001016918.1| (sphingosine kinase 1 [Xenopus tropicalis] >gi|89271372|emb|CAJ83231.1| sphingosine kinase 1 [Xenopus tropicalis])

HSP 1 Score: 196.438 bits (498), Expect = 2.738e-52
Identity = 134/403 (33.25%), Postives = 205/403 (50.87%), Query Frame = 0

Query:  206 QLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLY----QQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRM-SFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGP--PPRAAA---------------------LPPLRARLPDASGWVHMEHD-FLMVLISHVSHIAESAHSNPG-KRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYN--AVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRIL 576
             +AK W  RI E+A  +          G   S     RR LV+LNP AG G++   + + V P L +A     L+ T + ++A+ L+ + +   WD +V + GDGLL E+V GL  +   +C    + PLA++P G+GN +AAS  Y    +QA     +N    IL     VP DL  +  S G+R+ SFL+ ++GL++DVD+ESE  R+ GS R        +  LR+YR RLSYLP++  P   P + +                     L PL   +P    W  +  D F++VL+ + SH+     + P  +  G+G +Q+     +  +SR  L+ LFL +E+G H+      I N    A+RLE L   G+ T+DGE++PC S+QG+I     RI+
Sbjct:  117 NVAKTWANRIWEMAEEQ----------GPPSSILPPSRRFLVLLNPFAGTGKASGHFQTHVIPTLTEANATFTLLETERPKQAQELVRDEDLSGWDAIVVMAGDGLLFEVVNGLMERPDWACA--IKKPLAVLPGGSGNALAASVSYYSGHKQASGTKLLNNCTFILCKGQPVPLDLVSLTTSSGRRIFSFLSFAWGLISDVDIESERYRFMGSARFSFGTFVRLTALRTYRGRLSYLPARTSPDSTPESDSHRHNLNSSTDTVNPNVLEDSLLVPLNEPVPPH--WTTVTEDQFVLVLLLYQSHLGADLFTAPMVQSPGEGVMQLFYATSR--ISRASLLKLFLGMEKGSHMGESIPHITNVPVTAFRLEPLENVGIMTVDGESIPCCSLQGQIHKGLGRII 503          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|584026601|ref|XP_006807867.1| (PREDICTED: sphingosine kinase 2-like [Neolamprologus brichardi])

HSP 1 Score: 195.667 bits (496), Expect = 4.660e-52
Identity = 138/397 (34.76%), Postives = 215/397 (54.16%), Query Frame = 0

Query:  206 QLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLI----LKAKSTV-PADLSLVEASDGQRM-SFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGP-----------PPRA------AALPPLRARLPDASGW-VHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVE-IYNAV-AYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRIL 576
            Q A+RW   I++ + R++  SR+D +   +   R R  R+++++NP +GRGQ+  ++T  +  ML +A V + LV+T     A+ L+   +   WD +V + GDGLL E++ GL  ++        ++PL I+P G+GN +AAS ++  ++ P   N   L+    +  K  V P DL  V  +  QR+ SFL+L++G VADVD+ESE  R  G+ R  +  L  +  LR Y+ RL+YLP KE P           PP        + LP L   +PD   W V  E DF++VL  + SH+AE   + PG    DG + +  +     +SR  L+ LFLA+E+G H+       +Y  V A RLE +T +G+ T+DGE V  G +Q +I P   R++
Sbjct:  121 QEAERWAHAIRDASARQA--SRRDSV--AYMEVR-RPCRIMILVNPQSGRGQALQLFTGHIQGMLTEASVPYTLVITEHQNHARELVRKADLSQWDALVILSGDGLLFEVINGLMDRE--DWQEAIQIPLGILPGGSGNALAAS-VHHYSQSPPAWNEELLLSCGFMLCKGLVGPLDLVSVHLASKQRLFSFLSLAWGFVADVDIESEKYRHVGAIRFLMGTLVRLASLRVYQGRLAYLPVKEAPKLSKGSFTTNHPPSTPQAPVDSLLPDLEQPVPDT--WTVVKEEDFVLVLAIYQSHLAEDLWTVPGAMADDGLIHLFYV--TAGISRPALLRLFLAMEKGGHLACGCPHLVYEKVKALRLEPITPQGMITVDGEVVEYGPVQAQIHPGIARLM 505          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|928073676|ref|XP_013886703.1| (PREDICTED: sphingosine kinase 1-like [Austrofundulus limnaeus])

HSP 1 Score: 193.741 bits (491), Expect = 1.682e-51
Identity = 130/387 (33.59%), Postives = 204/387 (52.71%), Query Frame = 0

Query:  200 FMTPKAQL--AKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFL----ILKAKSTVPADLSLVEASDGQRM-SFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPPPRAAALPPLRARLPDASGWVHM-EHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAV--AYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRIL 576
               P+A L  A++W + ++E + ++  L      DG       R  R+L+++NP +G+GQ+  ++ + +  +L +AGV+  L+ T +   A+  +   +   WD +V + GDGLL E+V GL  +    C    + PL I+PSG+GN +AAS  Y     P     + L    +L        DL  V  S G R+ SFL+L++G VADVD+ESE  R  G+ R  +  L  +  LR Y+ RL+YLP+     P    LPPL    P +  WV + E DF++VL  + SH+AE   + PG R  DG + +  ++    +SR  L+ LFLA+E+G H+  +   + +A   A RLE  +  G+ T+DGE V  G +Q  +     RI+
Sbjct:  114 LQDPRANLEEAEKWARAVRERSAQQQALR-----DGVSLHSLPRPCRMLLLVNPQSGKGQALTLFKNHIQRLLSEAGVSLTLITTERQDHAREHVKEADLSQWDAIVIMSGDGLLYEVVNGLLERS--DCEEAIQTPLGILPSGSGNALAASVHYYSGAPPVTNEELLLSCGFLLCQGQVSRMDLVSVHLSSGPRLFSFLSLAWGFVADVDVESEKYRHLGAARFTVGTLVRLASLRVYKGRLAYLPAS--GEPSDLQLPPLDQ--PVSGDWVVVPEEDFVLVLAMYQSHLAEDLLAAPGARPDDGVIHLFYVK--AGISRAALLRLFLAMEKGTHLDTNCQHLVHAKVRALRLEPRSPEGIITVDGEVVEHGPLQAEVQRGRARII 487          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|156381134|ref|XP_001632121.1| (predicted protein [Nematostella vectensis] >gi|156219172|gb|EDO40058.1| predicted protein, partial [Nematostella vectensis])

HSP 1 Score: 188.348 bits (477), Expect = 6.750e-51
Identity = 105/337 (31.16%), Postives = 186/337 (55.19%), Query Frame = 0

Query:  243 RRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLYQQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRMSFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGPP----------PRAAALPPLRARLPDASGWVHMEHDFLMVLISHVSHIAESAHSNPGKRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYNAVAYRLE-SLTERGVYTLDGEAVPCGSIQGRI 568
            R++LV +NP +G+G+S  ++ + VAPM E A + ++LV+T  A  AK+    L    WD VV   GDGL+ E++ G  ++         ++P+ ++P+G+GN +  S L+   E    + A++ +++  +    D++ +     +  SFL++++G+++DVD+ESE  R+ G+ R  + A+  IL LR Y+ R+SYLP +E             P    LP L   LP  S W  +E  F++     +SH+     + P  + GDG L   V   +  +S+++L+ +F+ +E G H+  +E+    A A+RLE  LT+ G   +DGE +P  ++QG++
Sbjct:    5 RKMLVFVNPFSGKGKSLKIFRNKVAPMFENADIDYKLVITEYAGHAKAYASQLCIPEWDGVVICSGDGLVFEVLNGFMNRQ--DWEVAIKMPIGVIPTGSGNALCFSSLHASGEPMEVVCAIYAVIRG-NIHEMDIASIVTPTSRFYSFLSMTWGIMSDVDIESEKYRYLGNARFTVGAVVRILNLRLYQGRISYLPYEEDGQFTRSSGNQFGPELNLLPALEENLP--SSWKSVEGKFILCSSVMMSHLGPDICAAPQSKFGDGIL--YVAYAEAGISKMQLLDMFMKMEDGSHIDCEEIVCVRACAFRLEPDLTQLGTIAIDGELIPYSAVQGQV 334          

BLAST of EWM28757.1 vs. NCBI_GenBank
Match: gi|140833127|gb|AAI35625.1| (sphingosine kinase 1 [Xenopus tropicalis] >gi|1043408779|gb|OCA21414.1| hypothetical protein XENTR_v90025591mg [Xenopus tropicalis])

HSP 1 Score: 192.2 bits (487), Expect = 9.862e-51
Identity = 133/403 (33.00%), Postives = 204/403 (50.62%), Query Frame = 0

Query:  206 QLAKRWDQRIKELAWRESWLSRQDGLDGTHKSKRWRQRRLLVVLNPAAGRGQSQAVYTSVVAPMLEQAGVAHELVVTRKAQEAKSLMHNLNTEAWDCVVAIGGDGLLSEIVQGLYSQDPVSCHPLTRLPLAIVPSGTGNGMAASFLY----QQAEVPCPINAMFLILKAKSTVPADLSLVEASDGQRM-SFLALSFGLVADVDLESEVIRWAGSFRMDLFALYAILRLRSYRARLSYLPSKEGP--PPRAAA---------------------LPPLRARLPDASGWVHMEHD-FLMVLISHVSHIAESAHSNPG-KRMGDGTLQILVLRRQPSLSRLRLISLFLALERGYHVTADEVEIYN--AVAYRLESLTERGVYTLDGEAVPCGSIQGRILPQAMRIL 576
             +AK W  RI E+A  +          G   S     RR LV+LNP AG G++   + + V P L +A     L+ T + ++A+ L+ + +   WD +V + GDGLL E+V GL  +   +C    + PLA++P G+GN +AAS  Y    +QA     +N    IL     V  DL  +  S G+R+ SFL+ ++GL++DVD+ESE  R+ GS R        +  LR+YR RLSYLP++  P   P + +                     L PL   +P    W  +  D F++VL+ + SH+     + P  +  G+G +Q+     +  +SR  L+ LFL +E+G H+      I N    A+RLE L   G+ T+DGE++PC S+QG+I     RI+
Sbjct:  117 NVAKTWANRIWEMAEDQ----------GPPSSILPPSRRFLVLLNPFAGTGKASGHFQTHVIPTLTEANATFTLLETERPKQAQELVRDEDLSGWDAIVVMAGDGLLFEVVNGLMERPDWAC--AIKKPLAVLPGGSGNALAASVSYYSGHKQASGTKLLNNCTFILCKGQPVHLDLVSLTTSSGRRIFSFLSFAWGLISDVDIESERYRFMGSARFSFGTFVRLTALRTYRGRLSYLPARTSPDSTPESDSHRHNLNSSTDTVNPNVLEDSLLVPLNEPVPPH--WTTVTEDQFVLVLLLYQSHLGADLFTAPMVQSPGEGVMQLFYATSR--ISRASLLKLFLGMEKGSHMGESIPHITNVPVTAFRLEPLENVGIMTVDGESIPCCSLQGQIHKGLGRII 503