EWM28880.1, cds1798 (CDS) Nannochloropsis gaditana

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|585111312|gb|EWM28880.1| (2-isopropylmalate synthase [Nannochloropsis gaditana])

HSP 1 Score: 2926.73 bits (7586), Expect = 0.000e+0
Identity = 1417/1417 (100.00%), Postives = 1417/1417 (100.00%), Query Frame = 0

Query:    1 MLKCTKNTSNACQIFIKTRAATRIALDRSLQEQPYLRQGRNCSAAHPSAIRAIQGERYNQNTMHPSNLQPGLASSMAISSASSHGRRRQRVAVASSLLVLAAAAVSTSTTQAFILPIPPSAKSSPSFSSSAPRFARPNAVVSGKRVLRMATTLTDASSSDVISPAELDVVRPVPTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAGVLFLSTLSLGRFDLQEDRVVWQDGKECCVRRVVSSFRSSWRHTWITTLYEKAFRDYGYVKKTRARWKLPMKETIRSHPHVRGKYRDRLSMPSIDEEHAVHTALVPPTSAEVSIPSAGAASTAHPSSTEAMRSLQELEADDTEYSQTHTHPRPGHDHEKPPTAGILSFLYAFEGGDPDSEVDVVHAFQEEGGVPERKPPVFSFRTLLSYTGPGFLMCIAYLDPGNLEADLQVGAYTGYDLLWMLALATFMGFALQCMAVRLGVVTGKNLARVCKEQYNRATSMTLWILIELAIVGCDLQEIVGSAIAFQILFGFPLWLGCLITFIDTFTFLGIHYFGVRKLESVFVLLVAIMTVCFFINFGKSPPPSRDIAEGFVPQIRPYAVLSAVGLIGAVIMPHNIYLHSALVLSRKVDRRRPAHVKEANKYFAIDSAIALTVSFVINLAVVSVFAYHFFDHGCATAPGGPLACLSRTEEGVCDCQLIGLAGAHDALAEALGKSAKYIWAVGLLAAGQASTMTGTYAGQYVMEGFLDLQIPLWARVTFTRFVAIGPGLVVAILTENNQNLSDRFQEAINVFNSVLLSFSLLPVLHFTSSSVIMGEHVSSLPLTITIWVLAFGVLAINIYLVVLRIGAGQPWWVYVVTAVVGLIYLAFSYSLVQEDIENGAWAVVEGLPVGWPLKRRLLAWRERRKVRKEGLIGGDGISNVSPKHQRLIESVGDSDGSEPEST 1417
            MLKCTKNTSNACQIFIKTRAATRIALDRSLQEQPYLRQGRNCSAAHPSAIRAIQGERYNQNTMHPSNLQPGLASSMAISSASSHGRRRQRVAVASSLLVLAAAAVSTSTTQAFILPIPPSAKSSPSFSSSAPRFARPNAVVSGKRVLRMATTLTDASSSDVISPAELDVVRPVPTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAGVLFLSTLSLGRFDLQEDRVVWQDGKECCVRRVVSSFRSSWRHTWITTLYEKAFRDYGYVKKTRARWKLPMKETIRSHPHVRGKYRDRLSMPSIDEEHAVHTALVPPTSAEVSIPSAGAASTAHPSSTEAMRSLQELEADDTEYSQTHTHPRPGHDHEKPPTAGILSFLYAFEGGDPDSEVDVVHAFQEEGGVPERKPPVFSFRTLLSYTGPGFLMCIAYLDPGNLEADLQVGAYTGYDLLWMLALATFMGFALQCMAVRLGVVTGKNLARVCKEQYNRATSMTLWILIELAIVGCDLQEIVGSAIAFQILFGFPLWLGCLITFIDTFTFLGIHYFGVRKLESVFVLLVAIMTVCFFINFGKSPPPSRDIAEGFVPQIRPYAVLSAVGLIGAVIMPHNIYLHSALVLSRKVDRRRPAHVKEANKYFAIDSAIALTVSFVINLAVVSVFAYHFFDHGCATAPGGPLACLSRTEEGVCDCQLIGLAGAHDALAEALGKSAKYIWAVGLLAAGQASTMTGTYAGQYVMEGFLDLQIPLWARVTFTRFVAIGPGLVVAILTENNQNLSDRFQEAINVFNSVLLSFSLLPVLHFTSSSVIMGEHVSSLPLTITIWVLAFGVLAINIYLVVLRIGAGQPWWVYVVTAVVGLIYLAFSYSLVQEDIENGAWAVVEGLPVGWPLKRRLLAWRERRKVRKEGLIGGDGISNVSPKHQRLIESVGDSDGSEPEST
Sbjct:    1 MLKCTKNTSNACQIFIKTRAATRIALDRSLQEQPYLRQGRNCSAAHPSAIRAIQGERYNQNTMHPSNLQPGLASSMAISSASSHGRRRQRVAVASSLLVLAAAAVSTSTTQAFILPIPPSAKSSPSFSSSAPRFARPNAVVSGKRVLRMATTLTDASSSDVISPAELDVVRPVPTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAGVLFLSTLSLGRFDLQEDRVVWQDGKECCVRRVVSSFRSSWRHTWITTLYEKAFRDYGYVKKTRARWKLPMKETIRSHPHVRGKYRDRLSMPSIDEEHAVHTALVPPTSAEVSIPSAGAASTAHPSSTEAMRSLQELEADDTEYSQTHTHPRPGHDHEKPPTAGILSFLYAFEGGDPDSEVDVVHAFQEEGGVPERKPPVFSFRTLLSYTGPGFLMCIAYLDPGNLEADLQVGAYTGYDLLWMLALATFMGFALQCMAVRLGVVTGKNLARVCKEQYNRATSMTLWILIELAIVGCDLQEIVGSAIAFQILFGFPLWLGCLITFIDTFTFLGIHYFGVRKLESVFVLLVAIMTVCFFINFGKSPPPSRDIAEGFVPQIRPYAVLSAVGLIGAVIMPHNIYLHSALVLSRKVDRRRPAHVKEANKYFAIDSAIALTVSFVINLAVVSVFAYHFFDHGCATAPGGPLACLSRTEEGVCDCQLIGLAGAHDALAEALGKSAKYIWAVGLLAAGQASTMTGTYAGQYVMEGFLDLQIPLWARVTFTRFVAIGPGLVVAILTENNQNLSDRFQEAINVFNSVLLSFSLLPVLHFTSSSVIMGEHVSSLPLTITIWVLAFGVLAINIYLVVLRIGAGQPWWVYVVTAVVGLIYLAFSYSLVQEDIENGAWAVVEGLPVGWPLKRRLLAWRERRKVRKEGLIGGDGISNVSPKHQRLIESVGDSDGSEPEST 1417          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|551636547|ref|XP_005821098.1| (2-isopropylmalate synthase [Guillardia theta CCMP2712] >gi|428165114|gb|EKX34118.1| 2-isopropylmalate synthase [Guillardia theta CCMP2712])

HSP 1 Score: 750.355 bits (1936), Expect = 0.000e+0
Identity = 391/629 (62.16%), Postives = 484/629 (76.95%), Query Frame = 0

Query:   95 SSLLVLAAAAVSTSTTQAFILPIPPSAKSSPSFSSSAPRFARPNAVVSGKRVLRMATTLTDASSSDVISPAELDVVRPV---PTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAK-NGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIE--------KIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAG----VLFLSTLSLGRFDL 707
            SS L+L  + +S+  T +F+ P  P     P+      RF RP +       LRM             S  E+++ +P+   P++HD ETGRDP RVK+FDTTLRDGEQSPGC+MT++EK+QVAKQLA++GVDVIEAGFPIAS  DF AV++IA+ VGN ENPPIICGLARA + DI  C +AVK A FPRIHTFIATSDIHM HKL+KSREEVL IT++MVT A+SL +DVEFSAEDALRSD AFL QV+SVA++AGATT+NVPDTVG+TTP+EFK  I  LR + +G+E VTISVHGHDDLGMAVANF+ AI GGARQVE T+NGIGERAGNAA+EE+VMALHVRKS+YNP FGR  +   ALTNI   EI ++SRLV++LTGM++QPNKAIVGANAFAHESGIHQDG+LKNK TYEI+DA +IGL   +G+VLGKHSGR AF++RL +LG   L++ E NKAF+RFK++ADKKKEITN DLESIVN+E +    A +++++  QV CG S VPTAT+TL +++D+ E+T A+TGTGPVDAA+ AV +  GI+         +KL+E+ VSSVTAGIDALGEVTVRL+D  SG+++ GR+A+TDVV A     V  L+ L L R DL
Sbjct:   38 SSALLLPQSRLSS--TCSFVKPSSP-----PTARCRGMRFVRPGS-------LRM-------------SGGEMNM-KPINVKPSKHDLETGRDPTRVKVFDTTLRDGEQSPGCSMTSDEKMQVAKQLARMGVDVIEAGFPIASNDDFLAVKQIADVVGNYENPPIICGLARATEGDIRRCADAVKGARFPRIHTFIATSDIHMEHKLRKSREEVLKITKQMVTLAKSLVDDVEFSAEDALRSDRAFLAQVFSVAIKAGATTINVPDTVGFTTPTEFKETIEYLRKHTEGIEDVTISVHGHDDLGMAVANFLGAIEGGARQVEVTVNGIGERAGNAAMEEVVMALHVRKSYYNPMFGRPADSNDALTNIVLKEIHRSSRLVTSLTGMMVQPNKAIVGANAFAHESGIHQDGMLKNKLTYEIIDASTIGLDNSDGIVLGKHSGRAAFKARLADLGI-VLNEDETNKAFIRFKDLADKKKEITNADLESIVNDEAQ-DLAANRFKVMHVQVTCGISMVPTATVTLRDEKDDLELTDASTGTGPVDAAFQAVNRLCGIQGGGQANAADVKLMEYSVSSVTAGIDALGEVTVRLQDPRSGRVFLGRAADTDVVVASCVAYVNALNRLILQRSDL 636          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|1189389599|gb|OSX76579.1| (hypothetical protein BU14_0184s0006 [Porphyra umbilicalis])

HSP 1 Score: 744.577 bits (1921), Expect = 0.000e+0
Identity = 379/570 (66.49%), Postives = 448/570 (78.60%), Query Frame = 0

Query:  142 SGKRVLRMATTLTDASSSDV-ISPAELDVVRPVPTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLL--------------NKEDNQEVTVAATGTGPVDAAYVAVTKALG-IEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAGVL 695
            + +R L  A  ++ A +SDV ++P         PT+HD  TGRDP RVKIFDTTLRDGEQSPG T+T EEK+ +A+QLAKLGVDVIEAGFPIAS GDF AV  IAE+VGN  +PPIICGLARAL  DI+ CW A+K AAFPRIHTFIATSD+HM HKLKKSR EVL I + MVT+ARSLCEDVEFSAEDA+RSDP FLY+V+SVAV AGATTLNVPDTVGYTTP EF  LIRG+RANV GVE VTISVHGHDDLGMAVANF++A+  GARQ+ECTINGIGERAGNAALEE+VMALHVR+++YN R GR       LTNI  TEI++TSRLVS+LTGM++QPNKAIVGANAF+HESGIHQDG+LKN  TYEIMDA+S+GL  N +VLGK SGRHAFRSRLTELGYE LSD E+N+AFVRFKE+AD KK +++ D+ESIVN+E+R   DA +  L R QV CG+  VPT+T+TL                   + EVT +ATGTGPVDA + A+  A+G +  + L E+ VSSVT GIDALGEVTVR+ D ++G+ Y G S++TDVV A  +
Sbjct:   43 AAERPLMTAAKMSPAQASDVDVAPTAK------PTKHDLATGRDPNRVKIFDTTLRDGEQSPGATLTGEEKMIIARQLAKLGVDVIEAGFPIASVGDFRAVHAIAETVGNRPDPPIICGLARALTADIDRCWEAIKPAAFPRIHTFIATSDVHMEHKLKKSRAEVLAIAKAMVTHARSLCEDVEFSAEDAIRSDPEFLYEVFSVAVAAGATTLNVPDTVGYTTPFEFSKLIRGIRANVVGVENVTISVHGHDDLGMAVANFLSAVESGARQMECTINGIGERAGNAALEELVMALHVRRNYYNSRLGRVVGGP-PLTNINHTEIYQTSRLVSSLTGMLVQPNKAIVGANAFSHESGIHQDGVLKNPETYEIMDAKSVGLKDNSMVLGKLSGRHAFRSRLTELGYE-LSDEEINRAFVRFKELADTKKTVSSWDIESIVNDEVR-AVDAPRVALHRVQVSCGNLSVPTSTVTLSVVSGAAMPRMSGSPEPMADGEVTQSATGTGPVDATFKAINAAVGELVDVALEEYTVSSVTKGIDALGEVTVRVRDGATGRRYTGNSSDTDVVVASAM 603          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|546314367|ref|XP_005715553.1| (2-isopropylmalate synthase [Chondrus crispus] >gi|507112034|emb|CDF35734.1| 2-isopropylmalate synthase [Chondrus crispus])

HSP 1 Score: 743.421 bits (1918), Expect = 0.000e+0
Identity = 360/522 (68.97%), Postives = 438/522 (83.91%), Query Frame = 0

Query:  174 PTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKAL-GIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAGV 694
            PT+HD  TGRDP RVKIFDTTLRDGEQSPG ++TA+EK+ +A+QLAKLGVDVIEAGFPIAS GDF AVR IA+SVGN +NPPIICGLARAL+KDI  C+ AVK+AAFPRIHTFIATSD+HM +KLKK+REEV+ IT+E VTYARSLCEDVEFSAEDA+RSDP FL +V+S A+EAGATT+NVPDTVGYTTPSEF +LIR LR NV+G++ VTISVHGHDDLGMAVANF++A+  GARQ+ECTINGIGERAGNA+LEE+VMALHVR+ FYN R G+  + +  LTNI   EI  TSR+VS LTGM++QPNKAIVGANAFAHESGIHQDG+LK++ TYEIMDA+SIGL++N +VLGKHSGRHAFR+RL  +GYE ++D EL +AF RFKE+AD KKE++  DL+S+VN+E+RL  +A K  L R Q+ CG   +PTATI L+  +D+ E TV +TGTGPVD+AY A+ + +  I  + LLE+ VSSV+ GIDALGEV VR++D ++G  Y G +ANTD+V A V
Sbjct:   18 PTKHDIATGRDPDRVKIFDTTLRDGEQSPGASLTADEKMVIARQLAKLGVDVIEAGFPIASEGDFTAVREIAKSVGNRDNPPIICGLARALEKDISRCYEAVKHAAFPRIHTFIATSDLHMAYKLKKTREEVVEITKETVTYARSLCEDVEFSAEDAIRSDPDFLCEVFSAAIEAGATTINVPDTVGYTTPSEFASLIRYLRRNVRGIDDVTISVHGHDDLGMAVANFLSAVENGARQMECTINGIGERAGNASLEEVVMALHVRRQFYNARMGKDNKVDAPLTNIVHKEIHHTSRMVSNLTGMLVQPNKAIVGANAFAHESGIHQDGVLKHRQTYEIMDAQSIGLSENSIVLGKHSGRHAFRTRLVNMGYE-VTDEELERAFRRFKELADIKKEVSEADLQSLVNDEVRLVKEAVK--LTRIQIQCGYHIIPTATIGLILVDDDAEKTVTSTGTGPVDSAYNAINQVIEDIIHVTLLEYKVSSVSKGIDALGEVAVRVQDGATGNQYIGAAANTDIVVASV 536          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|545703726|ref|XP_005704188.1| (2-isopropylmalate synthase [Galdieria sulphuraria] >gi|452820628|gb|EME27668.1| 2-isopropylmalate synthase [Galdieria sulphuraria])

HSP 1 Score: 724.161 bits (1868), Expect = 0.000e+0
Identity = 350/522 (67.05%), Postives = 427/522 (81.80%), Query Frame = 0

Query:  174 PTRHDRETGRDPMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRL-GYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSAGV 694
            PT+HD  TGRDP RVK FDTTLRDGEQSPG T+T EEKL +A+QLAKLGVD+IEAGFP+AS GDF+AVR IA +VG  ENPPIICGLARA++ DI  CW AVK AAF RIHTFIATSDIHM HKLKKSR +VL      V YA+SLCED+EFSAEDA RSDP FLY+V+S A+EAGATT+NVPDTVGYTTP+EF +L+RG+R NV+G+E V +SVHGHDDLG+AV+NF++AI  GA+QVECT+NGIGERAGNA+LEEIVMAL+VR+ +YN + GR P+    LT + T EIWKTS+LVS LTGM++QPNKAIVG+NAFAHESGIHQDGILKN+ TYEIMDA  IG+ +N LVLGKHSGRHAFR RL ELG++ L++ ELN+AFVRFKE+ADKKKE+T  DLESIVN+E+RL G +  K E  R QV CGD  + TAT+T+++ E ++E+T  + GTGPVDAA+ A+    G+ K+ L E++V+S+T GIDALGEVTVR+   +  + + G +ANTD+V A V
Sbjct:  118 PTKHDLVTGRDPTRVKFFDTTLRDGEQSPGATLTGEEKLVIARQLAKLGVDIIEAGFPVASNGDFEAVRNIARTVGTRENPPIICGLARAVESDIARCWEAVKDAAFARIHTFIATSDIHMEHKLKKSRSQVLETVTRTVQYAKSLCEDIEFSAEDATRSDPEFLYEVFSRAIEAGATTINVPDTVGYTTPAEFGSLMRGIRRNVRGIENVCLSVHGHDDLGLAVSNFLSAIENGAQQVECTVNGIGERAGNASLEEIVMALYVRRQYYNNKLGR-PDPNALLTCVDTQEIWKTSKLVSNLTGMIVQPNKAIVGSNAFAHESGIHQDGILKNRLTYEIMDARLIGMQENQLVLGKHSGRHAFRKRLGELGFD-LTEDELNRAFVRFKELADKKKEVTLWDLESIVNDEVRLQGTEYLKLE--RVQVQCGDKLISTATVTIMHMELSRELTAVSIGTGPVDAAFKALNSIEGVPKVVLQEYMVNSITQGIDALGEVTVRIRGETDSRSFIGSAANTDIVVASV 635          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|299115810|emb|CBN74373.1| (2-Isopropylmalate synthase [Ectocarpus siliculosus])

HSP 1 Score: 691.034 bits (1782), Expect = 0.000e+0
Identity = 346/488 (70.90%), Postives = 403/488 (82.58%), Query Frame = 0

Query:  206 MTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKN-GLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692
            MT EEKL VAKQL KLGVD+IEAGFPIAS GDF+AV+ IA  VG   NPPIICGLARALKKDI+ C NAV  A FPRIHTFIATSDIHM HKLKKSRE+VL ITREMVT+ARS  +DVEFSAEDALRSD  FL +VYSVA+EAGATT+NVPDTVGYTTP EF  L+  LR  V+G++ VTISVHGHDDLGMAVANFM+AI GGARQVECTINGIGERAGNAALEEIVMALHVRK++YNP F R    E ALTNI+T EI K+S+LVS++TGM++Q NKAIVGANAF+HESGIHQDG+LKNK TYEIMDAE +GL ++  LVLGKHSGRHAFRSRL E GY +L+D ELN+AF+RFK++ADKKKEIT+LDL SIVN+E+R   +  +YEL+  QV  G +  PTAT+TL + +  +E  VAA GTGPVDAAY A+   +G    KLLE+ V+SVTAGIDALGEVTVR++D  S + Y G++A+TDV+ A
Sbjct:    1 MTREEKLMVAKQLGKLGVDIIEAGFPIASQGDFEAVQEIATVVGQGGNPPIICGLARALKKDIDVCANAVAPARFPRIHTFIATSDIHMKHKLKKSREQVLEITREMVTHARSHVDDVEFSAEDALRSDYDFLSKVYSVAIEAGATTINVPDTVGYTTPKEFMGLMHHLRGTVRGIDKVTISVHGHDDLGMAVANFMSAIEGGARQVECTINGIGERAGNAALEEIVMALHVRKAYYNPSFDRPVLSETALTNIKTKEIVKSSKLVSSMTGMMVQANKAIVGANAFSHESGIHQDGVLKNKETYEIMDAELVGLHQDTSLVLGKHSGRHAFRSRLQETGY-SLTDDELNRAFLRFKDLADKKKEITSLDLASIVNDEIR-DVNIRRYELVGMQVAAGTTNKPTATVTLYDTDLEKEGCVAAIGTGPVDAAYKAIDDIVGTRSTKLLEYSVASVTAGIDALGEVTVRVQDELSKREYIGKAADTDVIHA 486          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|493668026|ref|WP_006618368.1| (2-isopropylmalate synthase [Arthrospira platensis] >gi|291565673|dbj|BAI87945.1| 2-isopropylmalate synthase [Arthrospira platensis NIES-39] >gi|1015660302|gb|AMW26664.1| 2-isopropylmalate synthase [Arthrospira platensis YZ])

HSP 1 Score: 680.633 bits (1755), Expect = 0.000e+0
Identity = 340/508 (66.93%), Postives = 416/508 (81.89%), Query Frame = 0

Query:  185 PMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692
            P RV IFDTTLRDGEQ PG T+  +EKL +A+QLA+LGVDVIEAGF IAS+GDF+AV+RIAE+VG  EN PIIC LAR++K DIE    A+K AA PRIHTFIATSDIH+ +KLKK+R+EVL IT EMV YA+SLCED+EFS EDA RSDP FLYQV   A+ AGATT+N+PDTVGYTTPSEF  LIRG++ NV  ++   ISVHGH+DLG+AVANF+ A++ GARQ+ECTINGIGERAGNAALEE+VMALHVR+ F+NP FGR  + E+ +TNI T EI+KTSRLVS LTGM++QPNKAIVGANAFAHESGIHQDG+LKNK TYEIMDA+SIGL  N +VLGKHSGRHAF++RL ELG++ LSD E+NKAFVRFK+VADKKKE+TN D+E+IVN+E++   +  + EL+  QV CG++  PTAT+TL   E  QE+T AATGTGPVDA Y A+ + + +   +L+EF V SVTAGIDA+GEVT+RL     G+++ G +ANTD++ A
Sbjct:    7 PDRVIIFDTTLRDGEQCPGATLNVDEKLVIARQLARLGVDVIEAGFAIASSGDFEAVQRIAETVG-TENGPIICSLARSVKADIEAAAKALKPAAHPRIHTFIATSDIHLKYKLKKTRQEVLDITSEMVAYAKSLCEDIEFSPEDAGRSDPEFLYQVLERAIAAGATTVNIPDTVGYTTPSEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAALEELVMALHVRRQFFNPFFGRPADSEVPITNINTREIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQSIGLNDNLIVLGKHSGRHAFQNRLQELGFD-LSDDEINKAFVRFKDVADKKKEVTNWDIEAIVNDEIQQTPELFRLELV--QVSCGNNARPTATVTLRTPE-GQELTDAATGTGPVDAVYKAINRVVNVPN-ELIEFAVQSVTAGIDAIGEVTIRLR--YQGRVFSGHAANTDIIVA 506          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|647979219|gb|KDR56307.1| (2-isopropylmalate synthase [Arthrospira platensis str. Paraca])

HSP 1 Score: 680.248 bits (1754), Expect = 0.000e+0
Identity = 340/508 (66.93%), Postives = 416/508 (81.89%), Query Frame = 0

Query:  185 PMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692
            P RV IFDTTLRDGEQ PG T+  +EKL +A+QLA+LGVDVIEAGF IAS+GDF+AV+RIAE+VG  EN PIIC LAR++K DIE    A+K AA PRIHTFIATSDIH+ +KLKK+R+EVL IT EMV YA+SLCED+EFS EDA RSDP FLYQV   A+ AGATT+N+PDTVGYTTPSEF  LIRG++ NV  ++   ISVHGH+DLG+AVANF+ A++ GARQ+ECTINGIGERAGNAALEE+VMALHVR+ F+NP FGR  + E+ +TNI T EI+KTSRLVS LTGM++QPNKAIVGANAFAHESGIHQDG+LKNK TYEIMDA+SIGL  N +VLGKHSGRHAF++RL ELG++ LSD E+NKAFVRFK+VADKKKE+TN D+E+IVN+E++   +  + EL+  QV CG++  PTAT+TL   E  QE+T AATGTGPVDA Y A+ + + +   +L+EF V SVTAGIDA+GEVT+RL     G+++ G +ANTD++ A
Sbjct:    5 PDRVIIFDTTLRDGEQCPGATLNVDEKLVIARQLARLGVDVIEAGFAIASSGDFEAVQRIAETVG-TENGPIICSLARSVKADIEAAAKALKPAAHPRIHTFIATSDIHLKYKLKKTRQEVLDITSEMVAYAKSLCEDIEFSPEDAGRSDPEFLYQVLERAIAAGATTVNIPDTVGYTTPSEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAALEELVMALHVRRQFFNPFFGRPADSEVPITNINTREIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQSIGLNDNLIVLGKHSGRHAFQNRLQELGFD-LSDDEINKAFVRFKDVADKKKEVTNWDIEAIVNDEIQQTPELFRLELV--QVSCGNNARPTATVTLRTPE-GQELTDAATGTGPVDAVYKAINRVVNVPN-ELIEFAVQSVTAGIDAIGEVTIRLR--YQGRVFSGHAANTDIIVA 504          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|809068397|ref|WP_046319062.1| (2-isopropylmalate synthase [Arthrospira sp. TJSD091])

HSP 1 Score: 677.552 bits (1747), Expect = 0.000e+0
Identity = 339/508 (66.73%), Postives = 415/508 (81.69%), Query Frame = 0

Query:  185 PMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692
            P RV IFDTTLRDGEQ PG T+  +EKL +A+QLA+LGVDVIEAGF IAS+GDF+AV+RIAE+VG  EN PIIC LAR++K DIE    A+K AA PRIHTFIATSDIH+ +KLKK+R+EVL IT EMV YA+SLC+D+EFS EDA RSDP FLYQV   A+ AGATT+N+PDTVGYTTPSEF  LIRG++ NV  ++   ISVHGH+DLG+AVANF+ A++ GARQ+ECTINGIGERAGNAALEE+VMALHVR+ F+NP FGR  + E  +TNI T EI+KTSRLVS LTGM++QPNKAIVGANAFAHESGIHQDG+LKNK TYEIMDA+SIGL  N +VLGKHSGRHAF++RL ELG++ LSD E+NKAFVRFK+VADKKKE+TN D+E+IVN+E++   +  + EL+  QV CG++  PTAT+TL   E  QE+T AATGTGPVDA Y A+ + + +   +L+EF V SVTAGIDA+GEVT+RL     G+++ G +ANTD++ A
Sbjct:    7 PDRVIIFDTTLRDGEQCPGATLNVDEKLVIARQLARLGVDVIEAGFAIASSGDFEAVQRIAETVG-TENGPIICSLARSVKADIEAAAKALKPAAHPRIHTFIATSDIHLKYKLKKTRQEVLDITSEMVAYAKSLCQDIEFSPEDAGRSDPEFLYQVLERAIAAGATTVNIPDTVGYTTPSEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAALEELVMALHVRRQFFNPFFGRPADSEAPITNINTREIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQSIGLNDNLIVLGKHSGRHAFQNRLQELGFD-LSDDEINKAFVRFKDVADKKKEVTNWDIEAIVNDEIQQTPELFRLELV--QVSCGNNARPTATVTLRTPE-GQELTDAATGTGPVDAVYKAINRVVNVPN-ELIEFSVQSVTAGIDAIGEVTIRLR--YQGRVFSGHAANTDIIVA 506          

BLAST of EWM28880.1 vs. NCBI_GenBank
Match: gi|493672064|ref|WP_006622399.1| (MULTISPECIES: 2-isopropylmalate synthase [Arthrospira] >gi|375324498|emb|CCE20079.1| 2-isopropylmalate synthase [Arthrospira sp. PCC 8005] >gi|406714423|gb|EKD09588.1| 2-isopropylmalate synthase [Arthrospira platensis C1] >gi|585308762|emb|CDM98586.1| 2-isopropylmalate synthase [Arthrospira sp. PCC 8005])

HSP 1 Score: 676.781 bits (1745), Expect = 0.000e+0
Identity = 338/508 (66.54%), Postives = 415/508 (81.69%), Query Frame = 0

Query:  185 PMRVKIFDTTLRDGEQSPGCTMTAEEKLQVAKQLAKLGVDVIEAGFPIASTGDFDAVRRIAESVGNMENPPIICGLARALKKDIETCWNAVKYAAFPRIHTFIATSDIHMLHKLKKSREEVLGITREMVTYARSLCEDVEFSAEDALRSDPAFLYQVYSVAVEAGATTLNVPDTVGYTTPSEFKALIRGLRANVKGVEGVTISVHGHDDLGMAVANFMAAIRGGARQVECTINGIGERAGNAALEEIVMALHVRKSFYNPRFGRAPEDEMALTNIQTTEIWKTSRLVSTLTGMVIQPNKAIVGANAFAHESGIHQDGILKNKSTYEIMDAESIGLAKNGLVLGKHSGRHAFRSRLTELGYEALSDAELNKAFVRFKEVADKKKEITNLDLESIVNEEMRLGYDAGKYELLRCQVICGDSQVPTATITLLNKEDNQEVTVAATGTGPVDAAYVAVTKALGIEKIKLLEFVVSSVTAGIDALGEVTVRLEDLSSGKIYYGRSANTDVVSA 692
            P RV IFDTTLRDGEQ PG T+  +EKL +A+QLA+LGVDVIEAGF IAS+GDF+AV+RIAE+VG  EN PIIC LAR++K DIE    A+K AA PRIHTFIATSDIH+ +KLKK+R+EVL IT EMV YA+SLC+D+EFS EDA RSDP FLYQV   A+ AGATT+N+PDTVGYTTPSEF  LIRG++ NV  ++   ISVHGH+DLG+AVANF+ A++ GARQ+ECTINGIGERAGNAALEE+VMALHVR+ F+NP FGR  + E  +TNI T EI+KTSRLVS LTGM++QPNKAIVGANAFAHESGIHQDG+LKNK TYEIMDA+SIGL  N +VLGKHSGRHAF++RL ELG++ LSD E+NKAFVRFK+VADKKKE+TN D+E+IVN+E++   +  + EL+  QV CG++  PTAT+TL   +  QE+T AATGTGPVDA Y A+ + + +   +L+EF V SVTAGIDA+GEVT+RL     G+++ G +ANTD++ A
Sbjct:    7 PDRVIIFDTTLRDGEQCPGATLNVDEKLVIARQLARLGVDVIEAGFAIASSGDFEAVQRIAETVG-TENGPIICSLARSVKADIEAAAKALKPAAHPRIHTFIATSDIHLKYKLKKTRQEVLDITSEMVAYAKSLCQDIEFSPEDAGRSDPEFLYQVLERAIAAGATTVNIPDTVGYTTPSEFGGLIRGIKENVPNIDQAIISVHGHNDLGLAVANFLEAVKNGARQLECTINGIGERAGNAALEELVMALHVRRQFFNPFFGRPADSEAPITNINTREIYKTSRLVSNLTGMLVQPNKAIVGANAFAHESGIHQDGVLKNKLTYEIMDAQSIGLNDNLIVLGKHSGRHAFQNRLQELGFD-LSDDEINKAFVRFKDVADKKKEVTNWDIEAIVNDEIQQTPELFRLELV--QVSCGNNARPTATVTLRTPQ-GQELTDAATGTGPVDAVYKAINRVVNVPN-ELIEFSVQSVTAGIDAIGEVTIRLR--YQGRVFSGHAANTDIIVA 506