EWM28975.1, cds1700 (CDS) Nannochloropsis gaditana

Overview
NameEWM28975.1
Unique Namecds1700
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002457.1:1293263..1294126 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM28975.1
Product4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis
Orig transcript idgnl|cribi|Naga_100773g3.7445.mrna
GenethiJ
GbkeyCDS
Noteencoded by transcript variant 2; alternatively spliced
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002457.1supercontigCM002457.1:1293263..1294126 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Cellular Component
TermDefinition
GO:0016020membrane
Vocabulary: INTERPRO
TermDefinition
IPR002818ThiJ/PfpI
IPR029062Class_I_gatase-like
IPR006287DJ1
Homology
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|585111464|gb|EWM28975.1| (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis [Nannochloropsis gaditana])

HSP 1 Score: 579.326 bits (1492), Expect = 0.000e+0
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0
Query:    1 MCLSRSLFPCYFFPARSSSFPLRFSKTHMMTLSRLYFSTTAIAILCMYSIILPTSSAFLLRARPFPSPAATSTWSRCFLSSSSTPAAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN 287
            MCLSRSLFPCYFFPARSSSFPLRFSKTHMMTLSRLYFSTTAIAILCMYSIILPTSSAFLLRARPFPSPAATSTWSRCFLSSSSTPAAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN
Sbjct:    1 MCLSRSLFPCYFFPARSSSFPLRFSKTHMMTLSRLYFSTTAIAILCMYSIILPTSSAFLLRARPFPSPAATSTWSRCFLSSSSTPAAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN 287          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|585111463|gb|EWM28974.1| (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis [Nannochloropsis gaditana])

HSP 1 Score: 520.776 bits (1340), Expect = 0.000e+0
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0
Query:   29 MMTLSRLYFSTTAIAILCMYSIILPTSSAFLLRARPFPSPAATSTWSRCFLSSSSTPAAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN 287
            MMTLSRLYFSTTAIAILCMYSIILPTSSAFLLRARPFPSPAATSTWSRCFLSSSSTPAAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN
Sbjct:    1 MMTLSRLYFSTTAIAILCMYSIILPTSSAFLLRARPFPSPAATSTWSRCFLSSSSTPAAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN 259          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|553194432|ref|XP_005855953.1| (dj-1 family protein [Nannochloropsis gaditana CCMP526] >gi|422293096|gb|EKU20396.1| dj-1 family protein [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 404.831 bits (1039), Expect = 3.348e-141
Identity = 200/202 (99.01%), Postives = 201/202 (99.50%), Query Frame = 0
Query:   86 AAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN 287
            +APKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLP SWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN
Sbjct:    3 SAPKKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPRSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDYPGKGN 204          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|1129182245|gb|OLP89380.1| (Protein DJ-1-like B [Symbiodinium microadriaticum])

HSP 1 Score: 200.675 bits (509), Expect = 5.630e-61
Identity = 106/193 (54.92%), Postives = 134/193 (69.43%), Query Frame = 0
Query:   90 KVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDY 282
            KVLVPVADGSEEIE+  I D L R G  VTVASVMP           QV  SRG+ +VAD  I+DC G EWD IA PGG+PGAEHLRD  AL  LL+ Q    ++ AA+CASPAVV  THG L  +++TC+P  + V   A ++D +   V DGH++TS+GPGT+++FAL + ++LCG+ KA EVAKG+LVDY
Sbjct:    3 KVLVPVADGSEEIETTCITDTLTRCGCQVTVASVMPG--------KLQVKMSRGINVVADCAIEDCKGTEWDAIALPGGMPGAEHLRDCAALTELLKDQSGKGKVTAAVCASPAVVFGTHGLLP-EKSTCYPKFKDVVG-AGWQDAQ--AVMDGHIITSQGPGTSIQFALKIGEKLCGKDKAVEVAKGMLVDY 183          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|1088337091|gb|OHB86084.1| (dihydroxyacetone kinase [Planctomycetes bacterium RIFCSPHIGHO2_02_FULL_40_12])

HSP 1 Score: 200.29 bits (508), Expect = 7.232e-61
Identity = 106/194 (54.64%), Postives = 131/194 (67.53%), Query Frame = 0
Query:   89 KKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVDY 282
            KKVLVPVADGSEEIE+  I+DVL RA A VTVASV          +S Q+T SRG+KLVAD+ I +C+   +DLI  PGG+PGAEHLRDS  L+ +L+RQ    RL AAICASPAVVLH HG L  ++AT +P   F       E  +  VV DG+ +TSR PGT + FAL LV+ L G  KA E+A+ +LVD+
Sbjct:    3 KKVLVPVADGSEEIETACIVDVLRRAEADVTVASV----------NSLQITASRGMKLVADQLISECVQNTYDLIVLPGGMPGAEHLRDSKELVEMLKRQQNDGRLYAAICASPAVVLHHHGLLAQRKATAYPG--FAEQFENTEAIDSRVVVDGNCITSRAPGTAMEFALKLVELLYGEQKAREIAQSMLVDW 184          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|931396254|gb|KPJ85360.1| (dihydroxyacetone kinase [Spirochaetes bacterium DG_61])

HSP 1 Score: 200.29 bits (508), Expect = 7.235e-61
Identity = 110/192 (57.29%), Postives = 132/192 (68.75%), Query Frame = 0
Query:   89 KKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLV 280
            K+VL+P+ADGSEEIE+V IIDVL RAGA+VTVASV             QV  SRGVKL+AD  I +C+   +DLIA PGG+PGAEH+RDS  L  +L++Q K  RL  AICASPAVVL  HG L+GKRATC P   F   L   E+ +  VV DG+ VTSRGPGT + FAL LV+ L G  KA EVAK ++V
Sbjct:    3 KQVLIPIADGSEEIEAVCIIDVLRRAGASVTVASV----------DGLQVIASRGVKLIADNLISECVDRTFDLIALPGGMPGAEHMRDSRELTEMLKQQQKEGRLYGAICASPAVVLMHHGLLSGKRATCHP--DFSDRLENREEVDSRVVVDGNCVTSRGPGTAIEFALKLVELLFGSEKAAEVAKPMIV 182          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|1072840303|gb|OEU44596.1| (dihydroxyacetone kinase [Desulfobacterales bacterium S7086C20])

HSP 1 Score: 199.134 bits (505), Expect = 2.363e-60
Identity = 108/191 (56.54%), Postives = 133/191 (69.63%), Query Frame = 0
Query:   89 KKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLL 279
            K+VLVP+A+G+EE+E+VTI+DVL RAGA VTVASV          S  QVT SRGVKLVAD+ I DC    +DL+  PGG+PGAE+LRDSP L  LL+RQ K  RL  AICASPAVVL  HG L G+RATC+P   F + L   E  +  VV D   VTS+GPGT L F+LTLV+ L G+ KA E+A+ ++
Sbjct:    3 KEVLVPIAEGTEELEAVTIVDVLRRAGAVVTVASV----------SGLQVTASRGVKLVADRLIADCATNVYDLVVLPGGMPGAENLRDSPELEILLRRQQKEGRLFGAICASPAVVLEHHGLLAGRRATCYPT--FATKLKSAEAAQSRVVVDDKCVTSQGPGTALDFSLTLVELLFGKEKAGEIAEAMV 181          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|470423694|ref|XP_004337623.1| (DJ1 family protein [Acanthamoeba castellanii str. Neff] >gi|440794449|gb|ELR15610.1| DJ1 family protein [Acanthamoeba castellanii str. Neff])

HSP 1 Score: 196.052 bits (497), Expect = 2.426e-59
Identity = 105/192 (54.69%), Postives = 135/192 (70.31%), Query Frame = 0
Query:   89 KKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLV 280
            KKVLVP+A+GSEEIESV IID L RAGA VTVASV          ++ +VTCSR V+++AD  I DCLG  +DL+A PGG+PGAE LRDS  L  LL +Q ++ RL AA+CASP            K+ATC+PA++FV +L + ++ E+ VV DG+ VTSRGPGT L FAL LV++L G AKA+E+ K +LV
Sbjct:    2 KKVLVPIANGSEEIESVCIIDTLRRAGADVTVASV---------EATREVTCSRSVRILADALITDCLGNVYDLVALPGGMPGAERLRDSDQLKELLTQQKQSGRLYAAVCASP----------DDKQATCYPAQKFVDALKQKDNLEQRVVVDGNCVTSRGPGTALEFALQLVEQLYGAAKAQEIQKAMLV 174          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|298708461|emb|CBJ30585.1| (4-methyl-5(b-hydroxyethyl)-thiazole monophosphate biosynthesis protein, putative [Ectocarpus siliculosus])

HSP 1 Score: 197.593 bits (501), Expect = 4.287e-59
Identity = 105/193 (54.40%), Postives = 135/193 (69.95%), Query Frame = 0
Query:   89 KKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLARYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLVD 281
            K VLVPVADGSEEIESVTIID LVRAGA VTVASV P           +VTCSRGVK+ AD  I +C   +WD + CPGG+PGA  L+++  L  +L++Q+   R+V AICA+PAVVL +HG L GK+ATC+PA  F S + +     E VV D +++TS+GP T++ FAL LV  L G+ K++EVAKGLL +
Sbjct:   57 KHVLVPVADGSEEIESVTIIDTLVRAGALVTVASVGPEV---------EVTCSRGVKIKADCKIAECEARDWDAVVCPGGMPGAVSLKENETLEAILRKQNSEGRIVGAICAAPAVVLASHGLLEGKQATCYPASVFQSKIPQLVS--EKVVVDQNLITSQGPATSMAFALQLVGSLFGQEKSDEVAKGLLFN 238          
BLAST of EWM28975.1 vs. NCBI_GenBank
Match: gi|1148813952|ref|WP_077539720.1| (hypothetical protein [Phycisphaerae bacterium L21-RPul-D3] >gi|1146073943|gb|AQQ09196.1| Chaperone protein YajL [Phycisphaerae bacterium L21-RPul-D3])

HSP 1 Score: 194.126 bits (492), Expect = 1.709e-58
Identity = 105/193 (54.40%), Postives = 134/193 (69.43%), Query Frame = 0
Query:   89 KKVLVPVADGSEEIESVTIIDVLVRAGAAVTVASVMPSASTATLPSSWQVTCSRGVKLVADKGIDDCLGEEWDLIACPGGLPGAEHLRDSPALLTLLQRQDKAQRLVAAICASPAVVLHTHGFLTGKRATCFPAERFVSSLA-RYEDGEEGVVEDGHVVTSRGPGTTLRFALTLVDRLCGRAKAEEVAKGLLV 280
            K  L+P+ADGSEE+E+VTIIDVL RAG  +T+ASV          +   VT SRG KLVAD  I+ C G E+DLI  PGG+PGAEHLRDS  L  +L+ Q +A+RL AAICASPA+VL +HG + GK+ TC+P      SLA  Y +GE+ VV DG+ +TSRGPGT + FAL L + L G+  AE+ A+G+LV
Sbjct:    3 KTALLPIADGSEELEAVTIIDVLRRAGTELTIASV----------ADISVTMSRGTKLVADAPIEVCSGNEYDLIVLPGGMPGAEHLRDSRILTEMLKTQAEARRLYAAICASPAIVLASHGLMAGKKGTCYPG--LEHSLADSYLEGED-VVADGNCITSRGPGTAMAFALKLAELLEGKENAEKTAQGMLV 182          
The following BLAST results are available for this feature:
BLAST of EWM28975.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585111464|gb|EWM28975.1|0.000e+0100.004-methyl-5(b-hydroxyethyl)-thiazole monophosphate ... [more]
gi|585111463|gb|EWM28974.1|0.000e+0100.004-methyl-5(b-hydroxyethyl)-thiazole monophosphate ... [more]
gi|553194432|ref|XP_005855953.1|3.348e-14199.01dj-1 family protein [Nannochloropsis gaditana CCMP... [more]
gi|1129182245|gb|OLP89380.1|5.630e-6154.92Protein DJ-1-like B [Symbiodinium microadriaticum][more]
gi|1088337091|gb|OHB86084.1|7.232e-6154.64dihydroxyacetone kinase [Planctomycetes bacterium ... [more]
gi|931396254|gb|KPJ85360.1|7.235e-6157.29dihydroxyacetone kinase [Spirochaetes bacterium DG... [more]
gi|1072840303|gb|OEU44596.1|2.363e-6056.54dihydroxyacetone kinase [Desulfobacterales bacteri... [more]
gi|470423694|ref|XP_004337623.1|2.426e-5954.69DJ1 family protein [Acanthamoeba castellanii str. ... [more]
gi|298708461|emb|CBJ30585.1|4.287e-5954.404-methyl-5(b-hydroxyethyl)-thiazole monophosphate ... [more]
gi|1148813952|ref|WP_077539720.1|1.709e-5854.40hypothetical protein [Phycisphaerae bacterium L21-... [more]
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Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna1704rna1704Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications