EWM29010.1, cds1665 (CDS) Nannochloropsis gaditana

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|585111517|gb|EWM29010.1| (squalene epoxidase-like protein [Nannochloropsis gaditana])

HSP 1 Score: 1518.06 bits (3929), Expect = 0.000e+0
Identity = 750/750 (100.00%), Postives = 750/750 (100.00%), Query Frame = 0

Query:    1 MVRAIGLRFLEGDVWAVRRVRHSQLRRQESLARLAMLGSLAALWRAHEQVSPASAHMSTSFALLDTPQVPMLGMVLVVSLTCLCLVYVRASKCAAWTTPDEPSTKTRLNEIKADRSSPSFRSLPSGGGKEEDVATSNADRLFLSGGVGMQRVPPRLSPAAGQSIIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFGVIDPSPSSPAADCPTLSLSPPRFSEKEGDKLVHGAGRAVTPPFPEGTKVTPAPNPPSSPCGHLSMPPSPSFPSAARQGPTSLHPQGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR 750
            MVRAIGLRFLEGDVWAVRRVRHSQLRRQESLARLAMLGSLAALWRAHEQVSPASAHMSTSFALLDTPQVPMLGMVLVVSLTCLCLVYVRASKCAAWTTPDEPSTKTRLNEIKADRSSPSFRSLPSGGGKEEDVATSNADRLFLSGGVGMQRVPPRLSPAAGQSIIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFGVIDPSPSSPAADCPTLSLSPPRFSEKEGDKLVHGAGRAVTPPFPEGTKVTPAPNPPSSPCGHLSMPPSPSFPSAARQGPTSLHPQGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR
Sbjct:    1 MVRAIGLRFLEGDVWAVRRVRHSQLRRQESLARLAMLGSLAALWRAHEQVSPASAHMSTSFALLDTPQVPMLGMVLVVSLTCLCLVYVRASKCAAWTTPDEPSTKTRLNEIKADRSSPSFRSLPSGGGKEEDVATSNADRLFLSGGVGMQRVPPRLSPAAGQSIIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFGVIDPSPSSPAADCPTLSLSPPRFSEKEGDKLVHGAGRAVTPPFPEGTKVTPAPNPPSSPCGHLSMPPSPSFPSAARQGPTSLHPQGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR 750          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|298715207|emb|CBJ27879.1| (Squalene Monooxygenase (N-terminal region) [Ectocarpus siliculosus])

HSP 1 Score: 470.7 bits (1210), Expect = 5.396e-154
Identity = 299/593 (50.42%), Postives = 368/593 (62.06%), Query Frame = 0

Query:  163 SIIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVF---TPPQHKKEGGEKVQSILLKYPAYDPATLKELFGV-IDPSPSSPAADCPTLSLSPPRFSEKEGDKLVHGAGRAVTPPFPEGTKVTPAPNPPSSPCGHLSMPPSPSFPSAARQGPTSLHPQGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFST---PAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPY 748
             +II+GAGTAGA+AA   A +G KVLL ER MS QDRIVGELLQPGG+RALERLGLD CAKEG DSV VDGY +             G +  S++L YP  DP    ELFG  + P+ ++ A               K G+     AGR                     PC              +R+G     P+GRSFHN RFVQRLR  A AEPNV VV G VS LL  +       V+GV Y     R     +G+E EE+  R   APLT+VADG+WS LR   +   +P +LS+FVG+++ H   A+P+PH HCGHVVLA PSP+L+YQISSTETRVLVD+ G MPSA+ G L+ +L     PQLP + +  F  AVARG+ KSMPNR+MPA++V R G L++GDALNMRHPLTGGGMTVAL+DVE L  LL       + AA+     RF   R+A AATIN+LANAL+ VFST   PA   TR DL+ ACFDYLS+GG  + GP+ LLSGL+P P +L  HFF+VAFHG R++L PVP+ GG+ R Y +L VA +IIMPLL  E ST LA WP R  + L+ P+
Sbjct:   90 EVIIVGAGTAGASAAATFARQGRKVLLVERCMSEQDRIVGELLQPGGLRALERLGLDACAKEGTDSVVVDGYAIIRSAEAGGAAAAPGGRRDSVMLYYPESDPENRSELFGKGVKPAAAATA---------------KGGE-----AGR--------------------RPC--------------SREGEV---PRGRSFHNCRFVQRLREAAQAEPNVRVVEGTVSALLEGD----GGEVVGVRY-----REAAKGEGQEEEERVTREVRAPLTVVADGIWSGLRKCAN-TNRPQKLSTFVGVLVKHAADAAPVPHKHCGHVVLAHPSPILIYQISSTETRVLVDVAGAMPSAADGGLSAHLREKSAPQLPAMFRPAFLEAVARGDVKSMPNRAMPASSVHRPGALLLGDALNMRHPLTGGGMTVALKDVELLSELLR-GVPFRDAAAVASAAARFHAGRRAHAATINILANALYHVFSTPEDPASKGTRVDLQAACFDYLSLGGCYSAGPVGLLSGLSPVPWVLTTHFFMVAFHGCREHLLPVPTPGGVLRVYRLLHVATKIIMPLLEEEGSTFLAWWPFRKAIQLVLPW 614          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|698776666|ref|XP_009821377.1| (hypothetical protein H257_00395 [Aphanomyces astaci] >gi|574123502|gb|ETV88977.1| hypothetical protein H257_00395 [Aphanomyces astaci])

HSP 1 Score: 349.362 bits (895), Expect = 6.318e-107
Identity = 190/405 (46.91%), Postives = 260/405 (64.20%), Query Frame = 0

Query:  355 QGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEP-DSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDG-VLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGN-------PTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR 750
             GR FHNG FVQRLRAR LAEPNVTVV G VS+LL  +P  S TS   G++++       R A  ++VE   V  A APL + +DGLWS LR  +     P Q+SSFV ++++HP M + +P  + GHV+LA PSP+L+YQIS TETRVLVD+ G++PS+S GDL  +L TH+ PQ+P   +A F  AV  G  KSMPNR          G V+M+GD  NMRHPLTGGGM+VAL+DV  L  LL+ + DL +   +  ++  F ++R   ++T+N+LANAL+ +FS P+ +         R  LR++C++YL +GG    GP+ LLS LTP+P +LA HFF+VA +   + L P P+   L   +++   AC IIMPLL  E  T+LA  P+RAL+ +IFPY +
Sbjct:  237 HGRGFHNGAFVQRLRARMLAEPNVTVVEGTVSKLLLEQPAKSSTSVCTGIQFR-------RKALSDDVE-MPVETASAPLVIASDGLWSGLRRDLSTDV-PKQISSFVAILMTHPAMEATVPFRNFGHVILAHPSPILMYQISPTETRVLVDVPGKVPSSSNGDLTKHLLTHVAPQIPDASRAAFVRAVETGPVKSMPNREFMTTQPAHIGRVVMLGDTFNMRHPLTGGGMSVALKDVVLLNNLLK-NVDLSSGKDVAAVRRAFEQDRVHHSSTVNILANALYHIFSVPSADDDDKHVLAARATLRESCYEYLKMGGVFMAGPLGLLSALTPKPFVLATHFFMVAGYAAIRCLAPFPTPYRLYHCFQVFHQACVIIMPLLERENVTILAWAPLRALINVIFPYRK 631          

HSP 2 Score: 88.1965 bits (217), Expect = 2.309e-14
Identity = 49/108 (45.37%), Postives = 63/108 (58.33%), Query Frame = 0

Query:  164 IIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFGV 271
            +II+GAGTAG + AVGLA++G KVL+ E+ +  QDR VGEL+QPGG+ AL  L L ECA+   D V   GY +  P             I+L YP   P T  E  G+
Sbjct:  129 VIIVGAGTAGCSMAVGLAKQGRKVLVIEKSLEYQDRFVGELMQPGGLEALRSLDLIECAETSTD-VKTLGYSILLPHS---------DHIMLPYPDRAPHTFLEYMGL 226          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|673017420|ref|XP_008862671.1| (hypothetical protein H310_01361 [Aphanomyces invadans] >gi|574481874|gb|ETW08866.1| hypothetical protein H310_01361 [Aphanomyces invadans])

HSP 1 Score: 343.969 bits (881), Expect = 7.087e-105
Identity = 184/406 (45.32%), Postives = 258/406 (63.55%), Query Frame = 0

Query:  355 QGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPA---ANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGN---------PTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPY 748
             GR FHNG FVQRLRAR LAEPN+TVV G VS+L+      PT++  G +  +  G + R     +  E  V  A+APL + +DGLWS LR  + K  +P Q+SSFV L+++HP M + +P    GHV+LA PSP+L+YQIS TETRVLVD+ G++PS++ GDL  +L +H+ PQ+P   +A F  AV  G  KSMPNR       AN+ R  V+M+GD  NMRHPLTGGGM+VAL+DV  L  LL+ + DL +   +  ++  F ++R   ++T+N+LANAL+ +FS P+ +           R  LR++C++YL +GG+   GP+ LLS LTP+P +LA HFF+VA     + L P P+   +    ++   AC IIMPLL  E  T+ A  P+RA++ +IFPY
Sbjct:  239 HGRGFHNGAFVQRLRARILAEPNITVVEGTVSKLVL----EPTTK--GSQSNVCKGIQFRRKSPSDDVEMPVETALAPLVIASDGLWSGLRRDLSKD-QPKQISSFVALLMTHPAMEATVPFRQFGHVILAQPSPILMYQISPTETRVLVDVPGKVPSSTNGDLTKHLLSHVAPQIPEASRAAFIRAVETGPVKSMPNREFMTTQPANIGR--VVMLGDTFNMRHPLTGGGMSVALKDVVLLNELLK-NVDLSSGKEVAAVRRAFEQDRVHHSSTVNILANALYHIFSVPSTDDDVVDKAVLAARATLRESCYEYLKMGGFFMAGPLGLLSALTPKPVVLATHFFMVAGFAASRCLAPFPTPSRVYHCCQVFHQACIIIMPLLERENVTIFAWAPLRAMINVIFPY 634          

HSP 2 Score: 87.0409 bits (214), Expect = 5.103e-14
Identity = 47/107 (43.93%), Postives = 64/107 (59.81%), Query Frame = 0

Query:  164 IIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFG 270
            +I++GAGTAG + A+GLA++G KVL+ E+ +  QDR VGEL+QPGG+ AL  L L +CA+  +D V   GY +  P             I+L YP   P TL E  G
Sbjct:  131 VIVVGAGTAGCSIAMGLAKQGRKVLVVEKSLEYQDRFVGELMQPGGLEALRSLDLIDCAETSMD-VKTLGYSILRPHH---------DHIMLPYPNCAPKTLIEYMG 227          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|1173953066|gb|OQS00634.1| (squalene monooxygenase-like isoform X1 [Achlya hypogyna])

HSP 1 Score: 330.487 bits (846), Expect = 5.885e-100
Identity = 179/398 (44.97%), Postives = 243/398 (61.06%), Query Frame = 0

Query:  356 GRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPT---RKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR 750
            GR FHNG+FV RLRA+  A  NVT + G V++L+    D+ T    GV+Y+       R A  E+V+   V +A APL +V DGLWS LR  +    +P Q+SSF+ +++ HP M + +P    GHV+LA+PSP+++YQIS TETRVLVD+ G++PS S GDL  +L   + PQ+P    A F  AV  G  KSMPNR            +M+GD+ NMRHPLTGGGM+VALRD   L  LL    D  +  A+   +  F   R   ++TIN+LANAL+ VF+ P G      R+DLR++C+ YL +GG  + GP+ LL  LTP+P +LA HFF+VA +   + L PVP+ G L   Y +   AC IIMPLL  E  T+LA  P+  L+ +IFP+ R
Sbjct:  239 GRGFHNGKFVDRLRAKVFAHENVTCIEGTVTKLI---SDTKTDICRGVQYR-------RKAPAEDVD-MPVEQAKAPLVIVCDGLWSGLRREL-STDQPKQISSFIAILMKHPAMEASVPFRGYGHVILAEPSPIVVYQISPTETRVLVDVPGKVPSQSNGDLTKHLLEVVAPQMPEASHAAFIQAVNEGPVKSMPNREFMTTQPSLGRCIMIGDSWNMRHPLTGGGMSVALRDAVLLSDLL-GKVDFVDADAIAQARHTFEETRVNHSSTINILANALYHVFAIPDGYSNVAAREDLRESCYQYLKLGGMFSSGPLGLLCALTPKPFVLATHFFMVAGYAASRVLGPVPTPGRLLHCYRVFHEACMIIMPLLERENVTILAWTPLACLINVIFPFRR 623          

HSP 2 Score: 93.2041 bits (230), Expect = 5.537e-16
Identity = 57/138 (41.30%), Postives = 75/138 (54.35%), Query Frame = 0

Query:  141 LFLSGGVGMQRVPP--------RLSPAAGQSIIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFG 270
            ++L G VGM  V P           P     +IIIG GT G A A+GLA++G KVL+FE+ +  QDR VGEL+QPGG+ AL  L L +CA+   D +  DGY +       K+G E    ++L YP   P T  E  G
Sbjct:   96 VYLLGFVGMWAVKPAPKYTNDKYAEPNPDADVIIIGGGTTGCAMAMGLAKQGKKVLVFEKTLEYQDRFVGELMQPGGLDALRSLDLLDCAETETD-IKADGYYIL----KTKKGSE--DHVVLPYPERIPKTFLEYLG 226          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|669149884|ref|XP_008610504.1| (hypothetical protein SDRG_06501 [Saprolegnia diclina VS20] >gi|530737500|gb|EQC35742.1| hypothetical protein SDRG_06501 [Saprolegnia diclina VS20])

HSP 1 Score: 329.717 bits (844), Expect = 1.443e-99
Identity = 179/399 (44.86%), Postives = 243/399 (60.90%), Query Frame = 0

Query:  355 QGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPT---RKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR 750
             GR FHNG FV RLRA+  A  NVT + G V++L+    DS T    GV+Y+       R A  E+VE      A APL LV DGLWS LR  +   + P Q+SSF+ +++ HPPM + +P+   GHV+LA+PSP+++YQIS TETR LVD+ G++PS S GDL  +L   + PQ+P   +A F  AV  G  KSMPNR            +M+GD+ NMRHPLTGGGM+VALRD   L  LLE  TDL +  A+   +  F  +R   ++TIN+LANAL+ VFS P G      R+++R++C+ YL +GG  + GP+ LL  LTP+P++L  HFF+VA +   + L P P+   L   Y +   AC IIMPL+  E  T LA  P+  L+ L+FP+ R
Sbjct:  238 HGRGFHNGLFVDRLRAKVFAHENVTCIEGTVTKLV---SDSKTDICRGVQYR-------RKATSEDVE-MPTEHAKAPLVLVCDGLWSGLRRELSTES-PKQISSFIAILMKHPPMEASVPYRGYGHVILAEPSPIVVYQISPTETRALVDVPGKVPSQSNGDLTKHLLNVVAPQMPEASRAAFVTAVQDGPVKSMPNREFMTTQPSLGRCIMIGDSWNMRHPLTGGGMSVALRDAVLLTSLLE-KTDLVDADAIAAARHAFEEQRVNHSSTINILANALYHVFSIPDGYSNAAAREEIRESCYQYLKLGGMFSSGPLGLLCALTPKPAVLVAHFFMVAGYAASRVLTPFPTPSKLLHCYRVFHEACLIIMPLVLREDVTFLAWAPLAGLINLLFPFRR 623          

HSP 2 Score: 90.5077 bits (223), Expect = 3.941e-15
Identity = 57/138 (41.30%), Postives = 78/138 (56.52%), Query Frame = 0

Query:  141 LFLSGGVGMQRVPP-------RLSPAAGQS-IIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFG 270
            ++L G VGM  V P       + + A   + +IIIG GT G A A+GLA++G KVL+FE+ +  QDR VGEL+QPGG+ AL  L L +CA+   D +  +GY +       K+G E    I+L YP   P T  E  G
Sbjct:   96 VYLLGFVGMWAVKPTPKYTNDKYTEANRDADVIIIGGGTTGCAMAMGLAKQGKKVLVFEKTLEYQDRFVGELMQPGGLDALRSLDLLDCAETETD-IKAEGYTIV----KTKKGTE--DHIMLPYPERVPKTFSEYMG 226          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|813179020|ref|XP_012205979.1| (hypothetical protein SPRG_11641 [Saprolegnia parasitica CBS 223.65] >gi|641529138|gb|KDO23327.1| hypothetical protein SPRG_11641 [Saprolegnia parasitica CBS 223.65])

HSP 1 Score: 326.635 bits (836), Expect = 2.361e-98
Identity = 178/399 (44.61%), Postives = 241/399 (60.40%), Query Frame = 0

Query:  355 QGRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPT---RKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVEKSTVLAAWPVRALVGLIFPYGR 750
             GR FHNG FV RLRA+  A  NVT + G V++LL    DS T    GV+Y+       R A  ++VE      A APL LV DGLWS LR  +   + P Q+SSF+ +++ HPPM + +P+   GHV+LA+PSP+++YQIS TETR LVD+ G++PS S GDL  +L   + PQ+P   +A F  AV  G  KSMPNR            +M+GD+ NMRHPLTGGGM+VALRD   L  LLE  TDL +  A+   +  F  +R   ++TIN+LANAL+ VFS P G      R+++R++C+ YL +GG  + GP+ LL  LTP+P +L  HF +VA +   + L P P+   L   Y +   AC I+MPL+  E  T LA  P+ A + L+FPY R
Sbjct:  238 HGRGFHNGLFVDRLRAKVFAHENVTCIEGTVTKLL---SDSKTDICRGVQYR-------RKATSDDVE-MPTEHAKAPLVLVCDGLWSGLRRELSTES-PKQISSFIAILMKHPPMEATVPYRGYGHVILAEPSPIVVYQISPTETRALVDVPGKVPSQSNGDLTKHLINVVAPQMPEASRAAFVQAVQDGPVKSMPNREFMTTQPSLGRCIMIGDSWNMRHPLTGGGMSVALRDAVLLSSLLE-KTDLVDADAIAAARHAFEEQRVNHSSTINILANALYHVFSIPDGYSNAAAREEIRESCYQYLKLGGMFSSGPLGLLCALTPKPFVLVAHFCMVAGYAASRVLTPFPTPSKLVHCYRVFHEACLILMPLVLREDVTFLAWTPIAAFINLLFPYRR 623          

HSP 2 Score: 92.0485 bits (227), Expect = 1.204e-15
Identity = 58/138 (42.03%), Postives = 78/138 (56.52%), Query Frame = 0

Query:  141 LFLSGGVGMQRVPP-------RLSPAAGQS-IIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKKEGGEKVQSILLKYPAYDPATLKELFG 270
            ++L G VGM  V P       + + A   + +IIIG GT G A A+GLA++G KVL+FE+ +  QDR VGEL+QPGG+ AL  L L +CA+   D +  DGY +       K+G E    I+L YP   P T  E  G
Sbjct:   96 VYLLGFVGMWAVKPTPKYTNDKYTEANRDADVIIIGGGTTGCAMAMGLAKQGKKVLVFEKTLEYQDRFVGELMQPGGLDALRSLDLLDCAETETD-IKADGYTIV----KTKKGAE--DHIMLPYPERVPKTFFEYMG 226          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|1035915715|gb|OAY45307.1| (hypothetical protein MANES_07G049600 [Manihot esculenta])

HSP 1 Score: 301.212 bits (770), Expect = 1.115e-91
Identity = 173/375 (46.13%), Postives = 231/375 (61.60%), Query Frame = 0

Query:  356 GRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDI--FGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVE 728
            GRSFHNGRF+QR+R +A + PNV +  G V+ LL  +       + GV+YK  TG+ +               A APLT+V DG +S+LR  +    K    S FV LIL +      LP+P+ GHV+LADPSP+L Y+ISS E R LVDI    +MPS S G++ +YL + + PQ+PP L   F +A+ +G  ++MPNRSMPAA     G L++GDA NMRHPLTGGGMTVAL D+  L  LL    D  +  A+      F   RK  A+TIN LA AL+ VFST + +P +K++R+ACFDYLS+GG  + GPI LLSGL PRP  L +HFF VA +GV + ++P+PS   +     M+ VA  II P++  E
Sbjct:   14 GRSFHNGRFIQRMREKAASLPNVRLEEGTVTSLLEVK-----GTIKGVQYKTRTGQELT--------------ACAPLTIVCDGCFSNLRRSLCNP-KVDIPSCFVALILEN----CELPYPNHGHVILADPSPILFYRISSLEIRCLVDIPACHQMPSISNGEMTNYLKSKVAPQIPPELFDAFISAINKGNIRTMPNRSMPAAPHPTPGALLLGDAFNMRHPLTGGGMTVALSDIVVLRNLLRPLCDFSDAKALCEYLKSFYTLRKPVASTINTLAGALYKVFST-SHDPAQKEMREACFDYLSLGGVFSNGPIALLSGLNPRPLNLVIHFFAVAIYGVGRLIFPLPSAKRMWMGARMISVALGIIFPIIRDE 363          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|747082567|ref|XP_011088611.1| (squalene monooxygenase-like [Sesamum indicum])

HSP 1 Score: 305.834 bits (782), Expect = 1.820e-91
Identity = 180/374 (48.13%), Postives = 234/374 (62.57%), Query Frame = 0

Query:  356 GRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDIFG-RMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVE 728
            GRSFH+GRF+QRLR +A + PNV +  G V+ L+          V GV+YK        N DG++V      +A APLT+V DG +S+LR ++    +   +S FVG++L+       LPHP+ GHV+LA+PSPVL Y +SSTE R LVD+ G +MPS S GD+A YL T + PQLPP L   F   + +G+ +SMPNRSMPA+ +   G +++GDA NMRHPLTGGGMTVAL DV  L  LL    DLGN AA+      F   RK  A+TIN LA AL+ VF  PA +   +++R+ACFDYLS+GG  + GPI+LLSGL PRP  L  HFF VA +GV + L P PS   L     +L  A  II P++  E
Sbjct:  155 GRSFHHGRFIQRLREKAASLPNVRMEQGTVTSLI-----EEKGTVKGVKYK--------NKDGQDV------KAYAPLTIVCDGCFSNLRKNL-CIPQVNIVSHFVGMVLNLE--NGKLPHPNHGHVILANPSPVLFYPVSSTEVRCLVDVPGQKMPSISNGDMAKYLKTKVAPQLPPELHDAFIDQIDKGDIRSMPNRSMPASPLPTPGAVLLGDAFNMRHPLTGGGMTVALADVVVLRDLLRPVEDLGNAAALTKHLESFYALRKPVASTINTLAGALYKVF-CPAPDQASREMREACFDYLSLGGMYSQGPISLLSGLNPRPMSLVAHFFAVAIYGVGRLLLPYPSPKRLLLGVRLLLSAYGIIFPIVKSE 505          

HSP 2 Score: 79.337 bits (194), Expect = 1.053e-11
Identity = 41/82 (50.00%), Postives = 54/82 (65.85%), Query Frame = 0

Query:  163 SIIIIGAGTAGAAAAVGLAERGFKVLLFERDMSLQDRIVGELLQPGGVRALERLGLDECAKEGIDSVHVDGYVVFTPPQHKK 244
             ++I+GAG AG+A A  LA+ G  V + ERD+S  DRIVGELLQPGG   L  LG++EC   GIDS  V GY ++   ++ K
Sbjct:   61 DVVIVGAGVAGSALAYSLAKDGRIVQVIERDLSQPDRIVGELLQPGGYLKLVELGMEECV-HGIDSQQVYGYALYKEGKYAK 141          

BLAST of EWM29010.1 vs. NCBI_GenBank
Match: gi|1035915716|gb|OAY45308.1| (hypothetical protein MANES_07G049600 [Manihot esculenta])

HSP 1 Score: 301.212 bits (770), Expect = 2.487e-91
Identity = 173/375 (46.13%), Postives = 231/375 (61.60%), Query Frame = 0

Query:  356 GRSFHNGRFVQRLRARALAEPNVTVVNGIVSRLLYAEPDSPTSRVIGVEYKIGTGRRVRNADGEEVEEKEVRRAMAPLTLVADGLWSSLRTHVDKAAKPTQLSSFVGLILSHPPMASPLPHPHCGHVVLADPSPVLLYQISSTETRVLVDI--FGRMPSASTGDLAHYLSTHIQPQLPPVLQAPFAAAVARGEFKSMPNRSMPAANVDRDGVLMVGDALNMRHPLTGGGMTVALRDVEALCYLLETSTDLGNPAAMRVLKGRFARERKAWAATINVLANALHAVFSTPAGNPTRKDLRDACFDYLSVGGYCAGGPINLLSGLTPRPSILALHFFLVAFHGVRKYLWPVPSWGGLGRTYEMLQVACEIIMPLLSVE 728
            GRSFHNGRF+QR+R +A + PNV +  G V+ LL  +       + GV+YK  TG+ +               A APLT+V DG +S+LR  +    K    S FV LIL +      LP+P+ GHV+LADPSP+L Y+ISS E R LVDI    +MPS S G++ +YL + + PQ+PP L   F +A+ +G  ++MPNRSMPAA     G L++GDA NMRHPLTGGGMTVAL D+  L  LL    D  +  A+      F   RK  A+TIN LA AL+ VFST + +P +K++R+ACFDYLS+GG  + GPI LLSGL PRP  L +HFF VA +GV + ++P+PS   +     M+ VA  II P++  E
Sbjct:   44 GRSFHNGRFIQRMREKAASLPNVRLEEGTVTSLLEVK-----GTIKGVQYKTRTGQELT--------------ACAPLTIVCDGCFSNLRRSLCNP-KVDIPSCFVALILEN----CELPYPNHGHVILADPSPILFYRISSLEIRCLVDIPACHQMPSISNGEMTNYLKSKVAPQIPPELFDAFISAINKGNIRTMPNRSMPAAPHPTPGALLLGDAFNMRHPLTGGGMTVALSDIVVLRNLLRPLCDFSDAKALCEYLKSFYTLRKPVASTINTLAGALYKVFST-SHDPAQKEMREACFDYLSLGGVFSNGPIALLSGLNPRPLNLVIHFFAVAIYGVGRLIFPLPSAKRMWMGARMISVALGIIFPIIRDE 393