EWM29221.1, cds1523 (CDS) Nannochloropsis gaditana

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|585111741|gb|EWM29221.1| (polyketide synthase [Nannochloropsis gaditana])

HSP 1 Score: 6178.59 bits (16028), Expect = 0.000e+0
Identity = 2980/2980 (100.00%), Postives = 2980/2980 (100.00%), Query Frame = 0

Query:    1 MPLDEPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGRLILYMQGFYTVFASFSDNYKLCDLLWQPFAAPMPPPANELIAYSEEALAHTADWTSSDNSCMTAWLVERLRSNGALELPPPVGDAHANKSRKVKEVVPLVRKVLEALEARLSEVHDSAITDKVPAIPKVANLHERPEELVKTLTLKIQEKKVPAAEEGGADLVELTADEIKELVATSVQRSAETATVAAALKAQMEAQLRLLGHNCVFRVFEITQEATETVVEALSRLELPAHIIVEYFVGSHNSGLLKSLATKINRRATNIRLRRVLINDMLFNEIKDITFEAVVFDEWTRSGAAAYAEGRFESPAANALEALRTFMMPGAPVFVRGLYDLPLWVPLISLASASQLPLINTLSAEEAVADWTQQLLAHGKVYSTEVGATCVLIGEQGPALPRAPSRYLLFVDKQSHGEEFGRLLRLDHALAEAEVVLVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGDDIAHEEEEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDKKGGETADENDNHEEMMVLHNKAEMNSMLATLFEEDDDAANNHKAFHLRPLTSSAKLLFGATGLTGSHLLRYMIKAGVPKVFCIVRGSTSEGGLARIEKTMRLLKIWKPSFAANIVVIPGDMALTNFGIKPDMYARLVHEVDAVMHAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAATESDRAILRGMPYVQIKRRAEDILHFAARHYKLACFAYRIPLISVNTKCGFTGDFVAFSVMQTLYESRMMPKDMCESSCYPIMPADLAAKYVVRQMSRAPRKTNGKALLYSASAFSEIVSTEKLAEWVEELKNVKISRTATMEQVKEHYAKRLVNEVQDMQASFTDFLVALGRASERLRLQVRRESSLRFLVDKALYFNTKVPPSELLRDYIKNHPEVLLMEQAWFVDGEEDEGRDAEEGPGKDHPAEKDVA 2980
            MPLDEPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGRLILYMQGFYTVFASFSDNYKLCDLLWQPFAAPMPPPANELIAYSEEALAHTADWTSSDNSCMTAWLVERLRSNGALELPPPVGDAHANKSRKVKEVVPLVRKVLEALEARLSEVHDSAITDKVPAIPKVANLHERPEELVKTLTLKIQEKKVPAAEEGGADLVELTADEIKELVATSVQRSAETATVAAALKAQMEAQLRLLGHNCVFRVFEITQEATETVVEALSRLELPAHIIVEYFVGSHNSGLLKSLATKINRRATNIRLRRVLINDMLFNEIKDITFEAVVFDEWTRSGAAAYAEGRFESPAANALEALRTFMMPGAPVFVRGLYDLPLWVPLISLASASQLPLINTLSAEEAVADWTQQLLAHGKVYSTEVGATCVLIGEQGPALPRAPSRYLLFVDKQSHGEEFGRLLRLDHALAEAEVVLVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGDDIAHEEEEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDKKGGETADENDNHEEMMVLHNKAEMNSMLATLFEEDDDAANNHKAFHLRPLTSSAKLLFGATGLTGSHLLRYMIKAGVPKVFCIVRGSTSEGGLARIEKTMRLLKIWKPSFAANIVVIPGDMALTNFGIKPDMYARLVHEVDAVMHAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAATESDRAILRGMPYVQIKRRAEDILHFAARHYKLACFAYRIPLISVNTKCGFTGDFVAFSVMQTLYESRMMPKDMCESSCYPIMPADLAAKYVVRQMSRAPRKTNGKALLYSASAFSEIVSTEKLAEWVEELKNVKISRTATMEQVKEHYAKRLVNEVQDMQASFTDFLVALGRASERLRLQVRRESSLRFLVDKALYFNTKVPPSELLRDYIKNHPEVLLMEQAWFVDGEEDEGRDAEEGPGKDHPAEKDVA
Sbjct:    1 MPLDEPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGRLILYMQGFYTVFASFSDNYKLCDLLWQPFAAPMPPPANELIAYSEEALAHTADWTSSDNSCMTAWLVERLRSNGALELPPPVGDAHANKSRKVKEVVPLVRKVLEALEARLSEVHDSAITDKVPAIPKVANLHERPEELVKTLTLKIQEKKVPAAEEGGADLVELTADEIKELVATSVQRSAETATVAAALKAQMEAQLRLLGHNCVFRVFEITQEATETVVEALSRLELPAHIIVEYFVGSHNSGLLKSLATKINRRATNIRLRRVLINDMLFNEIKDITFEAVVFDEWTRSGAAAYAEGRFESPAANALEALRTFMMPGAPVFVRGLYDLPLWVPLISLASASQLPLINTLSAEEAVADWTQQLLAHGKVYSTEVGATCVLIGEQGPALPRAPSRYLLFVDKQSHGEEFGRLLRLDHALAEAEVVLVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGDDIAHEEEEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDKKGGETADENDNHEEMMVLHNKAEMNSMLATLFEEDDDAANNHKAFHLRPLTSSAKLLFGATGLTGSHLLRYMIKAGVPKVFCIVRGSTSEGGLARIEKTMRLLKIWKPSFAANIVVIPGDMALTNFGIKPDMYARLVHEVDAVMHAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAATESDRAILRGMPYVQIKRRAEDILHFAARHYKLACFAYRIPLISVNTKCGFTGDFVAFSVMQTLYESRMMPKDMCESSCYPIMPADLAAKYVVRQMSRAPRKTNGKALLYSASAFSEIVSTEKLAEWVEELKNVKISRTATMEQVKEHYAKRLVNEVQDMQASFTDFLVALGRASERLRLQVRRESSLRFLVDKALYFNTKVPPSELLRDYIKNHPEVLLMEQAWFVDGEEDEGRDAEEGPGKDHPAEKDVA 2980          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|585111904|gb|EWM29376.1| (polyketide synthase [Nannochloropsis gaditana])

HSP 1 Score: 5317.67 bits (13793), Expect = 0.000e+0
Identity = 2568/2983 (86.09%), Postives = 2750/2983 (92.19%), Query Frame = 0

Query:    4 DEPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGRLILYMQGFYTVFASFSDNYKLCDLLWQPFAAPMPPPANELIAYSEEALAHTADWTSSDNSCMTAWLVERLRSNGALELPPPVGDAHANKSRKVKEVVPLVRKVLEALEARLSEVHDSA-ITDKVPAIPKVANLHERPEELVKTLTLKIQEKK-----VPAAEEGGA---DLVELTADEIKELVATSVQRSAETATVAAALKAQMEAQLRLLGHNCVFRVFEITQEATETVVEALSRLELPAHIIVEYFVGSHNSGLLKSLATKINRRATNIRLRRVLINDMLFNEIKDITFEAVVFDEWTRSGAAAYAEGRFESPAANALEALRTFMMPGAPVFVRGLYDLPLWVPLISLASASQLPLINTLSAEEAVADWTQQLLAHGKVYSTEVGATCVLIGEQGPALPRAPSRYLLFVDKQSHGEEFGRLLRLDHALAEAEVVLVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGDDI--AHEEEEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDKKGGETADENDNHEEMMVLHNKAEMNSMLATLFEEDDDAANN---HKAFHLRPLTSSAKLLFGATGLTGSHLLRYMIKAGVPKVFCIVRGSTSEGGLARIEKTMRLLKIWKPSFAANIVVIPGDMALTNFGIKPDMYARLVHEVDAVMHAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAATESDRAILRGMPYVQIKRRAEDILHFAARHYKLACFAYRIPLISVNTKCGFTGDFVAFSVMQTLYESRMMPKDMCESSCYPIMPADLAAKYVVRQMSRAPRKTNGKALLYSASAFSEIVSTEKLAEWVEELKNVKISRTATMEQVKEHYAKRLVNEVQDMQASFTDFLVALGRASERLRLQVRRESSLRFLVDKALYFNTKVPPSELLRDYIKNHPEVLLMEQAWFVDGEEDEGRDAEEGPGKD 2972
            DE IAVIGASIVLPANI+ RE+FFEI+R KNQVSVDTVAA RYHASDIDD+D  HPWKL+SKYSM+FTEEEL  YDSHLFNIQGKVSRNMHLQQLGVLQS F+GLEDAGIPLH+IYRTRTG+FVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRR+ DAE+HGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEY   EPVRRA+LTSASSLWALP EDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGP ISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDS+YWVDNVRRPVLFQKAIETIFSQDT PDIVIEIGPHKTLCSPIAQVLSA+GKSA +LCPLKRNDTCS+RFLE+LGQ+FEKGL VDCK WYQDLGY F+EDLPKHPFIK+RLHE+HPVLKNDMRNG+YIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSL+ALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKV+K DLL EDGSI+ GFKPEAYGLLA + LKDIG+ GLK+LIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTG+L+L+MQGF+TVFASF+DNYKLCDLLWQPFAAP       L AY++EAL HT  WT++DN  +T+WL+E+LR+NGALELP P  +    K  K KE VPLVRKVLEALEAR+SE+ +S   + K   +P +A L ER  EL+  L  KI+E+K     V  A+E  A   D V++TA+E+KEL + ++Q  AET  VA +LK Q E Q +LLGHN VFRVFEI QEATETVVEA SRL+LP++IIVEYFVGSHN+GLLKSLATK+NR+ATN+RLRRVLIND LFNE+KDITFEAVVFDEWTRSGAAA+ EG+ E+P ANA+EA+R+FMMPGAP++VRGL ++PLW  L SL SA+ LPL N L AEEA+A+WTQ  L  G+V+  +VGA  +L+G      P AP+RYLL VD+Q+ GEE  R ++  +  AEAE++LVGE G++TDEST+DDYA L+A++L P+  EE+ E      A EE E P  +PFKGV+F AGL+D+SVIGDVAFSRLLKL Q LLKNGDLLK+LR +E+AEP SLWV+TKGAYVG++ PAQG+VQG+ATVLCGEL DMVTKHVD+ASS+ KD+  L++LV+ D+REKSYTV+ G AQVARFNA+DKT+ARA+QV ATD KL YFADI S ASVPGEI VT+QLRDVP PKENEVQIDI AAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVV+A+G+DV DL+VGDRVMTGEP CFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVI+ATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPI+TFPMNE++EALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAH ISPPVKSIFHVAGVSVDIVLPEMKPE+FYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVL+TSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGS+DKKGGET  E+ N + MMVLHNKAEMNSM+ TLFE+ +             L  L S AKLLFGATGLTG+HLLRY+IKAG+PKVFC+VRG+T+EGGLARIEK MRLLKIWKPSFAA+IV +PGDMA  NFG+KP++YARLV EVDAV+HAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAAT+ DR +LR MPYVQIKRRAEDILHFAARHYKL C AYRIPLISVNTK GFTGDFVAFSVMQTL ES MMPK+MCE SCYPIMPAD+AAKY+VRQMSRAPRKTNG ALLYSA+A+SEI+ST+KL EWVEEL+ +KI R A+M+ +KE+Y KRLV +V DMQASFTDFL ALGRASERLR QVRRES++R LVD+AL++N KV PSELL+DY+++HPEVLLMEQAWF D EE+E  DA   P  D
Sbjct:    6 DESIAVIGASIVLPANIHDREKFFEIVRAKNQVSVDTVAAQRYHASDIDDTDMNHPWKLRSKYSMVFTEEELNCYDSHLFNIQGKVSRNMHLQQLGVLQSVFQGLEDAGIPLHEIYRTRTGVFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRLVDAEQHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYKKVEPVRRAVLTSASSLWALPAEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPAISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSNYWVDNVRRPVLFQKAIETIFSQDTCPDIVIEIGPHKTLCSPIAQVLSALGKSATVLCPLKRNDTCSLRFLESLGQVFEKGLMVDCKAWYQDLGYNFEEDLPKHPFIKKRLHEMHPVLKNDMRNGVYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLVALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVIKTDLLQEDGSIRSGFKPEAYGLLADINLKDIGEDGLKNLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGQLVLFMQGFFTVFASFTDNYKLCDLLWQPFAAPEMTTEAALHAYAKEALTHTESWTATDNDRLTSWLIEQLRANGALELPAPAVEDAKPKKGKGKEAVPLVRKVLEALEARMSEIQESTEESKKSVGLPTIAKLDERASELITALVAKIKEEKEAAKPVTEADEEDADESDFVQITAEEMKELTSAAIQLPAETLAVATSLKMQAEKQQQLLGHNYVFRVFEIAQEATETVVEAFSRLDLPSNIIVEYFVGSHNAGLLKSLATKVNRKATNLRLRRVLINDTLFNELKDITFEAVVFDEWTRSGAAAFMEGKLEAPVANAVEAVRSFMMPGAPIYVRGLNNIPLWTALTSLVSATPLPLTNALPAEEALAEWTQLFLVQGEVHQVKVGAGTLLMGRLTVPAPAAPARYLLVVDEQAEGEEMLRRMQARNPAAEAEIILVGEKGKMTDESTSDDYATLIAELLTPKKIEEVSEATSAVPAEEESEEPVASPFKGVVFSAGLNDSSVIGDVAFSRLLKLGQALLKNGDLLKSLRENEVAEPASLWVVTKGAYVGELRPAQGSVQGMATVLCGELHDMVTKHVDMASSSGKDLAALAELVLADAREKSYTVVEGVAQVARFNAIDKTDARALQVSATD-KLCYFADIKSTASVPGEIGVTFQLRDVPAPKENEVQIDIHAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVTAVGKDVTDLQVGDRVMTGEPCCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIYATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIMTFPMNEMREALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHEISPPVKSIFHVAGVSVDIVLPEMKPEDFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLITSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSSDKKGGETEGEDANQDGMMVLHNKAEMNSMITTLFEDGEKLVVQPLKRGNMSLSSLGSGAKLLFGATGLTGAHLLRYLIKAGIPKVFCVVRGATAEGGLARIEKAMRLLKIWKPSFAAHIVALPGDMASPNFGLKPELYARLVLEVDAVVHAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAATDKDREVLRAMPYVQIKRRAEDILHFAARHYKLNCLAYRIPLISVNTKGGFTGDFVAFSVMQTLSESHMMPKEMCEKSCYPIMPADMAAKYIVRQMSRAPRKTNGHALLYSAAAYSEIISTQKLGEWVEELQGIKIDRLASMDNMKEYYRKRLVPQVLDMQASFTDFLAALGRASERLRQQVRRESTVRNLVDRALFYNPKVLPSELLKDYVRDHPEVLLMEQAWFKDEEEEEEEDAGAHPEAD 2987          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|585108747|gb|EWM26659.1| (type i polyketide synthase [Nannochloropsis gaditana])

HSP 1 Score: 3326.57 bits (8624), Expect = 0.000e+0
Identity = 1676/2996 (55.94%), Postives = 2173/2996 (72.53%), Query Frame = 0

Query:    6 PIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDN-EPVTVGVNSFGIGGAYGHVVMQEYTGP-EPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMADVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKT-WGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGRLILYMQGFYTVFASFSDNYKLCDLLWQPFAAPMPPPANELIAYSEEALAHTAD--WTSSDNSCMTAWLVERLRSNGALE----LPPPVGDAHANKSRKVKEVV--PLVRKVLEALEARLSEVHDSAITDKVPAIPKVANLHERPEELVKTLTLKIQEKKVPAAEEGGAD----LVELTADEIKELVATSVQRSAETATVAAALKAQMEAQLRLLGHNCVFRVFEITQEATETVVEALSRLELPAHIIVEYFVGSHNSGLLKSLATKINRRATNIRLRRVLINDMLFNEIKDITFEAVVFDEWTRSGAAAYAEGRFESPAANALEALRTFMMPGAPVFVRGLYDLPLWVPLISLASASQLPLINTL-SAEEAVADWTQQLLAHGKVYSTEVGATCVLIGEQGPALPRAPS-RYLLFVDKQSHGEEFGRLLRLDHALAEAEVVLVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGDDIAHEE----EEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMN-GAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQ-RVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNY--LEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSR-GSNDKKGGETADENDNHEEMMVLHNKAEMNSMLATLFEEDDDAAN--NHKAFHLRPLTSSAKLLFGATGLTGSHLLRYMIKAGVPKVFCIVRGSTSEGGLARIEKTMRLLKIWKPSFAANIVVIPGDMALTNFGIKPDMYARLVHEVDAVMHAGGSRKWHMDTESVACNISGLMNIITLARKNNASVHYVSSGWLDAEEAATESDRAILRGMPYVQIKRRAEDILHFAARHYKLACFAYRIPLISVNTK----CGFTGDFVAFSVMQTLYESRMMPKDMCESSCYPIMPADLAAKYVVRQMSRAPRKTNGKALLYSASAFSEIVSTEKLAEWVEELKNVKISRTATMEQVKEHYAKRLVNEVQDMQAS-FTDFLVALGRASERLRLQVRRE-SSLRFLVDKALYFNTKVPPSELLRDYIKNHPEVLLMEQAWFVDGEEDEGRDAEE 2967
            P+A+IG +I LPAN+ SRE  F++I++K Q+SVDTVA GRYHA+D+D+ D  HPWKLKSK +M FT EE+ +YD+H+FNIQGK+++ MH+ QL  LQ+A+EGLEDAGIPLH+I++TRTG+FVA Y  FLP+ D PDET LR   MSS+AD VSYFLGT+GPSVTLETACSSSLVA+A+A  +L  GDCDTA+V G+NYL+EKDFHLSLQACGV+VQGP  HPFDEDG KG+ R EGMG VVLRRM+DAE+ G+R+L K+ RA+AASAG ADN+L+G GRVYEQPCP GMR M+ R +  A +P  +L+YMECHATGTAVGDVIE+ A+G VFG+SH+L  NP+RIAS+KSNIGHAEVAAGIFSVIKIVQMMKHR +LP+AGVT PR DF+W  +NM +QQE EPFP+  EPV  G++SFGIGG+YGHV+++EY GP   + +       +   LP         + PLSAVS+ HL+LFA+RMA Y+ E+  T++L+DLC TMW++R+RF++RK+F+AS+ + L + L+ FA+SGN +P+GEGRKMQVAYVFTGQGSQWPGVGK+LMAFPVY+++V+A DK+FK  +GWSILEK +TLSA+EMRDT+YAQPISF+VQVGL+ELLKFF+ FPDVV GHSAGEVAAAYA+GLLTLEEA  +VYHRS EQQKMAGCGRLM VG+  E++ + I G  D+E+ACVNSPESVVLASSE RL E+  L PEGT    V GNIAFHSSR+ P+L ++K RL+FLDKR +T W LP++S VTGK+E+ +D++YW  NVR PVLFQKA+ET F+ DT PD+V+EIGPH+TL SPI QVL A GK+AA+   LK+   C++RFLEALG +FE+G  +D + WY  + Y F+ED+PKHPFIK+  +E+ P ++ DMR G + DGPVAG Q+FFDGA++ EVSDRT+K M  H+MGG+ I PGMFFVEM LEA +  P+TL NVEFK+M +IP AS GE PS+I L F++ +D IRSF VRS P R+K DE+PV V HCTG  +K+ LL +DGSI  GFKPEAYG++ GL+L+DIG+ GLK+L+  H+ C+H S E FYG HCVEG ME+L  FQL++S H+DP   +IL K+ YDN  W + GG+FGV+ LD+++H  L+LA  SA+YYAGGFDA HFLRQPAT ELYV+   DK R+ IGRK+K+GDFA+YD  G LI+Y+QGF+++  S  DNYKLC+LLWQP+ A     A   +A      A T D  WT++ N  +T++LV+ L + G L            A +   RK +  V  PL ++V EA+E    E   S ++   PA PK   L E    L + +  KI+     A++    +     +ELT +++ EL+ATS  + +E A V+AALK  + A   +LG N VFR+ E+ +E   TV EALS LELP  + VE FVGS N+GLL SLA KI +RA N+ LRRVL+++    E+KDITF+AVV +EW R+G+AA+AEG+     +NA+  L  F+ P A VFVRGL D+PLWV L+S  S + LP  +     + A  +WT  L   G   +  + A  +L+G     LP   + RYL+  D    G +  R +   ++   AEV++VGE G LTD S+ ++Y + +  ++   +  E  +  DI  +     EE     PFKGV+F AGL+D S +G+ AF RLLKLAQ LLK  +           +P+SLW++T G Y+G++ PAQG +QG +TVL  EL  + TKHVDLA + +  +  L++LV+ ++ E +Y V   G   V RFN +DK  AR+  VLA + K+S+FAD+    + PG+I   ++L+++P PK +EV +D+ AAALNFRDVMIALSLLPEKSYE S+YG+NLGLEASGVV+A+G  VK+LKVGD+V+  EPRC  NRLNAPA RVV     ++ FEDAAS+QSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGA I+ATAG++EKRQLV+DMGV  V+NSR+  WFDDLMR+T+G+GVDV+LNSLAGKHQRLG+QALRSSGRFLEIGKMDIFDNNGL LLAFRKNISFFAIDMDR+ LD+P    E+  EVG +F  G+YD +P   FPM+++KEALE MKTGKHVGK+VLTNY  ++  KT +  P  V VEKPQNIFH +ATYL+TGGAGGFGS +VRYA+ +GA+ FLITTRSA+TEKV+  F D++ +  V +EV+T DTG E D+K L  +A  +SPP KSIFHVAGVSVD++LPEMKPEN+ EVA+CKA GAW LH+ +LD+ +ENFV VSSIA+LIGG GM+SY++SNAYLDALMR RRSQGL  AAFNM+SLSDVGIL NNLSARKFQLK+G+E+++S RA++ LE GL+ GMNPI+TMFFKE+TR MFP Q+ WS+ LGEA++LGAVAA   FM+ KEIA FL++EI+ ITG + VLVT+QL  LGLDS GFVELGGR+KKHF +E+ +VKMSPD TLEDV+K+I+KLQ+R G N K    +AD+     ++ VLH+KAEMNSM+++ FE  D   +    K   L  L +S+ LLFGA  L G+H+LRY+IK G   V+C+   +T+EGGL +IE++MR L +WK SFA +I+ + GD++  +FG+K   Y  L   VD ++H+GGS KW MD + V  NISGLMN+I +ARK  AS+HY+SS  L A E A   DR ILR +PY  +KR+AEDIL FAARHY L CF YR+P I+ N K      +  D V    MQ + +S + P     +    ++ AD AAK+VVR M   P    G A+ Y  +  S  +    LA+WVE      + R AT+  ++ + +KR   E+  + +S F D L AL R   R   +   E S+ + ++  A    T++   +  + Y++    +L +E+    D +ED   +A+E
Sbjct:   58 PLALIGTAITLPANVKSREELFQVIKDKRQLSVDTVAGGRYHATDVDEVDE-HPWKLKSKEAMAFTMEEVLAYDAHMFNIQGKLTKTMHVHQLAALQAAYEGLEDAGIPLHEIFKTRTGVFVAGYCPFLPSVDAPDETFLRSSAMSSIADHVSYFLGTHGPSVTLETACSSSLVAMALAANALSLGDCDTAVVVGINYLSEKDFHLSLQACGVLVQGPACHPFDEDGPKGFFRAEGMGCVVLRRMSDAERLGNRVLVKLARAVAASAGAADNSLDGAGRVYEQPCPNGMRNMYMRAFSSAGIPYRRLNYMECHATGTAVGDVIEVDAVGSVFGESHDLETNPIRIASIKSNIGHAEVAAGIFSVIKIVQMMKHRVYLPTAGVTKPRTDFDWDANNMRVQQEIEPFPEGGEPVVAGISSFGIGGSYGHVILEEYRGPVGSMVKPAAALPPAATDLP------SNYILPLSAVSLAHLKLFAERMAAYVGEKKDTLALRDLCGTMWVNRTRFKFRKAFIASSLENLQQSLSTFAASGNVQPAGEGRKMQVAYVFTGQGSQWPGVGKSLMAFPVYKESVEAVDKIFKGLAGWSILEKAETLSAEEMRDTVYAQPISFIVQVGLFELLKFFRAFPDVVTGHSAGEVAAAYAAGLLTLEEAVLIVYHRSQEQQKMAGCGRLMVVGVPHERVLEYISGQDDIEVACVNSPESVVLASSEARLNEIKGLFPEGTALTFVQGNIAFHSSRMIPVLESIKKRLSFLDKRGETGWSLPFISTVTGKVETDVDTEYWCKNVRYPVLFQKALETAFAGDTVPDVVLEIGPHQTLVSPIKQVLGATGKNAAVFPTLKKGAPCALRFLEALGNLFERGAELDLEPWYAAMNYCFEEDMPKHPFIKKAFYEILPTMRFDMRFGQFHDGPVAGKQRFFDGAFLVEVSDRTFKGMLGHRMGGKTILPGMFFVEMALEASRTYPVTLANVEFKAMCRIPVASKGEIPSMIGLTFSDVKDDIRSFEVRSAPIRDKADEDPVYVTHCTGLAIKSKLLEKDGSIYKGFKPEAYGIMGGLKLQDIGEAGLKELVDLHSDCKHVSKEAFYGHHCVEGVMEWLNGFQLIQSAHLDPADKTILAKIKYDNRRWVQTGGVFGVEFLDSVIHPLLMLADDSAVYYAGGFDAGHFLRQPATDELYVHLIPDKSRMVIGRKQKIGDFAVYDSLGTLIMYIQGFFSIIGSIIDNYKLCNLLWQPYDAAF---AKAPLAIEAAPAAATDDSTWTAAKNEDLTSFLVKELETTGVLSPEAVAAAAADVAASTNGRKPRGPVKPPLSQQVREAIE---REGTPSRVSSAAPAFPKAGGLDEIGVGLAQAIAAKIKPSVSVASDSLATNEEEKPLELTGEDVGELIATSTFQVSEAARVSAALKELILANQEVLGKNYVFRILELAEERVHTVFEALSLLELPTCVTVEVFVGSFNAGLLMSLANKIAKRAPNVLLRRVLMSEKPEKELKDITFDAVVLNEWIRTGSAAFAEGKSAKAPSNAILYLAPFLAPSAQVFVRGLIDVPLWVTLVSQVSTAALPFADVKDQVKNARDEWTDALALSGGFKA--LAADSLLVGTI-KGLPTVQAGRYLVVADHLQAGTDLRRRIGALNSACVAEVIVVGEGGSLTDSSSVEEYESAINGLVGSDSSCEESDFSDITTDSSTAVEEGLGALPFKGVVFLAGLNDRSQVGEAAFGRLLKLAQALLKCTE-----------KPSSLWLVTNGVYMGEIKPAQGVIQGFSTVLKSELNGVATKHVDLADTET-GLGPLAQLVLANAAEMTYAVSEAGEVLVPRFNTVDKAAARSDSVLANNTKVSFFADV-EKQTTPGKIGAKFRLQELPAPKADEVTVDVYAAALNFRDVMIALSLLPEKSYEASFYGKNLGLEASGVVTAVGSGVKNLKVGDKVLLNEPRCLTNRLNAPAYRVVPFGGLKMSFEDAASIQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGATIYATAGSEEKRQLVRDMGVTHVFNSRSTEWFDDLMRETDGKGVDVILNSLAGKHQRLGIQALRSSGRFLEIGKMDIFDNNGLDLLAFRKNISFFAIDMDRMVLDNPTLATEVAIEVGKNFEAGNYDLIPTQVFPMDKMKEALELMKTGKHVGKIVLTNYTKVDKGKTARYEPLPVVVEKPQNIFHENATYLITGGAGGFGSAMVRYAFDHGAKHFLITTRSANTEKVKASFSDLIKNPGVTLEVVTTDTGKEEDVKALCAKAKAMSPPTKSIFHVAGVSVDVLLPEMKPENYVEVADCKARGAWFLHENSLDMHLENFVCVSSIAALIGGPGMASYSASNAYLDALMRYRRSQGLAAAAFNMASLSDVGILKNNLSARKFQLKVGMEFMSSTRAMEMLETGLMVGMNPIVTMFFKEQTRDMFPSQAAWSHQLGEAISLGAVAASNSFMSAKEIAAFLADEIRSITGVTTVLVTAQLTALGLDSLGFVELGGRMKKHFNVEMDAVKMSPDMTLEDVAKMIFKLQARSGGNGKADASSADQG---ADLHVLHSKAEMNSMISSRFERADSIKHIPPAKLLRLNSLRASSVLLFGAEYLNGAHVLRYLIKGGTRTVYCVGPWATTEGGLIKIERSMRELALWKDSFAKHIIPVTGDVSKKDFGMKGPAYDELAASVDTIVHSGGSLKWAMDADLVPTNISGLMNVIAMARKTGASIHYLSSSSLTAIENAERQDRDILRPVPYFDVKRKAEDILQFAARHYNLRCFIYRLPFITPNMKGRFGTAYAKDLVLVRFMQCIQDSGLFPSTEMGNHVLYVISADAAAKFVVRHMRHIPTSAKGTAVRYFVTNESTGLPMSSLADWVEGKTGRPLDRNATVADMRAYMSKRFKYEINLVYSSIFFDMLPALCRGGARSTAKTLHEVSAFKQIIRHASRVRTQMDNVDAFKSYVQQERTLLQIEEDILRDQDEDLTEEAKE 3021          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|585108307|gb|EWM26301.1| (polyketide synthase [Nannochloropsis gaditana])

HSP 1 Score: 1169.84 bits (3025), Expect = 0.000e+0
Identity = 606/1270 (47.72%), Postives = 835/1270 (65.75%), Query Frame = 0

Query:    3 LDEPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDID-------DSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRK------NPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPDNE---PVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQ--ELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLMAFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMA-----DVEIACVNSPESVVLASSEERLKEVGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLP-----YVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIF-SQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAE-EQDG--IRSFVVRSCPTR---EKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEKKGGIFGVQLLDALLHQTLILATHSA--IYYAGGFDACHFLRQPATKELYVYFTLDKYRLQIGRKKKVGDFALYDGTGRLILYMQGFYTVFASFSDNYKLCDLLWQ 1235
            L + IAV G +I LP +I++ ++ F++++ K Q+  D VA  RY AS +D       DSDS +PW+L+S+++ +FT+EE+ SYD H F +  K++R+M ++QLG+LQS +  LEDAG+P   +YRTRTG+F A Y     A + PDET LRG +MSSL+D V++FLGT+GPSVTLETACSSSLVA+++AVA+L++GDCD A+V  +N   +KDFHLSLQACGV+    TSHPF++ G KGY+R EG G +VLRR  D    GDR+LC +  A+A SAG  +    G GR+YE PC +GM+E+ +R Y+ A +    + YMECHATGT VGDV+EL+AIG++ G    LRK      N LR+ASVKSNIGHAEV+AG+FS+ K+++MM+ R +LP+AGVT PR DF+W  HNM + Q  EPF   E   PV +GVNSFGIGG+Y HV+++EY  P+       +SA  + A     A    L+ PLSAVS  HL+L+A  +  Y +   P + ++D+C TM ++R+ FQYRK+ V        L  +L    S  +++   E +  ++ + FTGQG+QW G G+TLMA PVYR+AV   D LF++ SG+SILEK  TLSA+ +R+T +AQP++FL+QVG+ +LL+   ++P +V GHSAGEVA+AYA+GLLTL+EA +VVYHRS EQQK+AGCGRL+AVGL + ++ D +  +       VEIACVNSP   VLA  E  L +V   LP GT  A V GNIAFHSS V+PILP ++ RL FLD+  +          +VS VTG+  ++ D++YW  NVR  VLFQ A++ IF  +DT PD+V+EIGPHKTL  P+ Q + A G+ A +L  L RN  C    ++    +FE  + ++ +  Y  L Y+   D+PKHPFIK R+HEL  +   ++ +GLY+ GPVAG  K   GAY  EV DRT+K M DHKMGGQ I PG F+VEM LEA  G P  LTNVEFKSM KIP +SSG  P+L++++F + E  G  I+SF+VRS   +    + DE     +HCTG  +K     E+   +   +  A+GLLA  +L+DIG+ GL  L   HTQC  SSTE+ YG    EG  EY  SFQ++ S+H +    + L  + +DN  W ++GG FG+QLLDA+     +L +H    + YAGGF+A HFLR+P++ +LYV+   D     +GRKK  GD  +YD  G L+L ++    +F + +D  KL +L+WQ
Sbjct:   24 LQDRIAVNGYAIQLPGDISTGDQLFDLVQAKRQLRQDVVAGKRYPASMVDEGEGSVNDSDS-NPWRLRSRFANLFTDEEVESYDCHFFRVTDKLARSMDIRQLGILQSVWHALEDAGLPAERLYRTRTGVFAAGYVQVEHAQEAPDETTLRGSLMSSLSDHVAFFLGTHGPSVTLETACSSSLVALSLAVAALRKGDCDYAIVACVNIPQDKDFHLSLQACGVLSTTGTSHPFEDAGPKGYVRAEGYGCIVLRRFRDTIYDGDRVLCTIRNAVAGSAGAVEGLATGAGRMYESPCVWGMKELMARAYEGAGLSPALVSYMECHATGTYVGDVLELEAIGEILG-MPRLRKEDEEGHNLLRVASVKSNIGHAEVSAGLFSLAKVIEMMRRRMYLPTAGVTTPRTDFDWEGHNMRVIQTTEPFGPLESTPPVLMGVNSFGIGGSYAHVIVEEYRPPK-------SSADRILA---RHAFEGQLLLPLSAVSNAHLKLYAHELLTYFQNT-PEVDIRDVCGTMAVNRTAFQYRKALVVGGQDLPGLLLQLKGIVSGPDSQAPEEEKGKRILFAFTGQGAQWMGCGETLMAVPVYREAVLEVDALFQALSGYSILEKAQTLSAETLRETQHAQPVTFLIQVGIVKLLESSGIWPSIVTGHSAGEVASAYAAGLLTLDEAVQVVYHRSQEQQKLAGCGRLLAVGLGQNEVMDFLATLELSQDNAVEIACVNSPSGTVLAGVEVALNQVKAALPPGTFSAFVQGNIAFHSSHVDPILPNIRCRLTFLDRPDRVLARTNISPVFVSTVTGEESTQTDAEYWCRNVRSAVLFQHAVQCIFEDEDTAPDVVLEIGPHKTLAGPVLQTIKAAGREALVLPTLTRNGHCLKVMMDLFAMLFESNVKMNFETLYHALSYSV-VDIPKHPFIKTRMHELVHIHNRELVSGLYLKGPVAGSYK--SGAYEVEVCDRTFKSMMDHKMGGQEILPGCFYVEMALEAT-GLPCALTNVEFKSMCKIPRSSSGNPPTLVSISFKDVEGSGGEIQSFLVRSTSAKIAVCERDEPLSYTDHCTGLAIKR---GEEYDTRCQMRSGAFGLLAPWKLEDIGEKGLATLKKHHTQCLASSTEHAYGIINKEGLSEYGPSFQVISSLHGNEVDDTFLCVVEFDNVEWNRQGGSFGIQLLDAMYQLLFLLPSHDPQIVVYAGGFEAAHFLRKPSSNKLYVFLDHDSTIPYVGRKKSGGDLLVYDENGVLLLAIKAINIIFGASTDTTKLSELVWQ 1273          

HSP 2 Score: 848.581 bits (2191), Expect = 0.000e+0
Identity = 484/1151 (42.05%), Postives = 698/1151 (60.64%), Query Frame = 0

Query: 1411 GHNCVFRVFEITQEAT-ETVVEALSRLELPAHIIVEYFVGSHNSGLLKSLATKINRRATNIRLRRVLINDMLFNEIKDITFEAVVFDEWTRSGAAAYAEGRFESP------AANALEALRTFMMPGAPVFVRGLYDLPLWVPLISLASASQLPLINTLSAEEAVADWTQQLLAHGKVYSTEVGATCVLIGEQGPALPRAPSRYLLFVDKQSHGEEFG-RLLRLDHALAEAEVVLVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGDDIAHEEEEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVTKHVDLAS------SNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDR-AHGIS--PPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAK--GDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDKKGGETADE 2542
            G   VFRV E+ ++++  T  EAL RL L  H I+E F+GSH+   L  +A  ++     +R+R++L+   L  E+ D  F+ V    W  SG  A+ +    +       A N +  L +FM PG+  +V GL                          ++A  D  Q L    +  +T V A  +      P LP +   Y++  +          RL  L  +     VV  G + +   +  A  +   L  VLA                   I     F+G++F  GL D +++G +AFSRL KL Q ++     +  L  ++  +   LW++T+GAY G + P QG +QG   V+  ELP +    +D+A+      + ++ +  +++++   +REK Y + N +  V R+  +D +  R+  VL TD  ++Y  D+++  S PG++NV +Q  DV  P  ++V +DI AAALNFRDV+IA+++LPE S+E SYYGR+LG+EA+G+VSA+G +V  LKVGDRV T E  CF NRLNAPA+RVVKL + + FE AAS QSVYNT+HHAL+ +ARI+KG+ VLIH+AAGG+GHAAIS+ +H+GA+++ATA    KR  V+++GVE++Y+SR+  WF ++MRDTN +GVD VLNSLAGKHQ+LG+QAL   GRFLEIGK+DI+DN  L LLA RKN  F AIDMDRLALDDP  T +   EV      G Y  +P   FPM+ I++ +E +K GKH GKVVLTNY         +P  V V  PQ +FH +ATYL+TGGAGGFGSK+VR+A+  GAR F+IT  +   E+V  LF D++  +   +E +  DT S  DM  +  + A G S  PP+K+I HVAGVS+D VL ++  ++  +V +CKALGAW+LH+ T DL IE FVV+SS+ASL+GG G ++Y+S+NA++DAL+R RRS GLPG AFNM+SLSDVGILAN++  RK QL+  VE++ S RALQ+LE  +V  +     +FF+E    ++P ++ + +      TLGA AAK     +T+KE+ + +++ IK I+   EVL  S L ++G+DSF  VEL  RIK+ FG+E++  K+ P  T+ DV+K+IY  QS   +++K      E
Sbjct: 1342 GSPTVFRVLEVVEDSSLATFAEALGRLTLDPHQIIEVFIGSHDIQALNMVAGTMSGAERGLRVRKILM-PTLDRELADYAFDVVALHAWHTSGKMAWQQSDSNTTSGETQRALNVVTTLESFMTPGSQFWVLGLDG----------------------GEKKAWVDVLQPLSPDLRCTTTLVAARMM------PRLPTSQRTYVVIAENSQEAALLTKRLTLLAGSACSTIVVTCGANAKAHFQDNAKCWETSLESVLA------------------SISVDDGFQGLVFLGGLDDETILGTMAFSRLAKLCQAVMACEQKITALM-NDSKQNGQLWIVTEGAYAGKIRPNQGCIQGFTYVIAHELPTLEAYLIDMATDEVTGMTRAQGLEKVAEILAYMAREKCYLISNESVLVPRYFPIDMSARRSRLVLPTDSNVAYCCDVSTGLSTPGQVNVLFQSHDVKQPGPDQVTVDIHAAALNFRDVLIAINMLPELSFEGSYYGRHLGMEAAGLVSAVGTNVTHLKVGDRVATAEASCFGNRLNAPANRVVKLVESISFEVAASTQSVYNTAHHALVTIARIKKGDRVLIHAAAGGVGHAAISVCQHIGAIVYATASAG-KRATVQNLGVEYIYDSRSTSWFSNVMRDTNYRGVDAVLNSLAGKHQQLGIQALAPGGRFLEIGKLDIYDNAKLGLLALRKNGLFVAIDMDRLALDDPELTIKTTSEVFSLLSTGAYRPIPTTIFPMDRIRDTIELIKAGKHTGKVVLTNYTAHGN----NPVPVEVRMPQRVFHPNATYLITGGAGGFGSKMVRWAFARGARHFVITV-TKRPERVATLFSDLIATSGCTLETVIADTASRKDMADVVCKYAQGTSMRPPLKAILHVAGVSLDTVLTDVTADDLLKVGSCKALGAWYLHELTKDLSIETFVVISSVASLMGGRGRAAYSSANAFMDALIRHRRSLGLPGTAFNMTSLSDVGILANDIKVRKMQLRSNVEFVRSARALQDLEDAIVCQLPLACELFFRENAVGVYPNRASFLHGGTAQFTLGADAAKTADRALTSKEVLNLVADTIKSISRHKEVLGCSALFSVGMDSFSTVELISRIKQTFGVEINPSKIGPATTVADVAKMIYSQQSGHKSERKASSENQE 2438          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|460501335|gb|AGH13577.1| (type I polyketide synthase [bacterium symbiont of Plakortis simplex pPS11G3])

HSP 1 Score: 542.732 bits (1397), Expect = 9.001e-153
Identity = 396/1232 (32.14%), Postives = 602/1232 (48.86%), Query Frame = 0

Query:   59 PWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAY---TAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKV-LRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLP-SAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVAYVFTGQGSQWPGVGKTLM-AFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGM-----------ADVEIACVNSPESVVLASSEERLKE-VGELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDM--RNGLYIDGPVAGVQKFFDGA-YVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYG--THCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAW---EKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDACH--FLRQPATKELYVY-------FTLD---KYRLQIGRKKKVGDFALYDG-TGRLILYMQGFYTVFASFSDNYKLCD-------LLWQP-FAAPMPP 1243
            P +  S Y  +  +E     D  LF +      NM  Q   +L  A+E  E AG  LH +  + TG+F+ A    TA   A  G +E  +     S LA+++SY L   GPS+   TACS+SL A+ M + +++QGDCD  LV    YL       S  A GV+      H FD D A GY+R EG     ++ +  AE+ GD I   +   A+  +    D A    GR    P  +   ++       A +      Y+E HATGT VGD IE  AI + FG     R  PLR+ASVKSN+GH E AA   +++K+V MM+HRTF P S     P  + ++    M +Q  CEPFPD+ PV VG+NSFG GGA GH V++EY    P           +W++P+  A   G + PLSA ++  L   A  + + + ER     L  L   +   RSRF  R +F     ++LAE L AF        + E  + +VA VF+GQG+QW G G+ L  A PV+R+ V A ++ ++  SG S+ +   T S +E+ +   AQP  F++Q  L EL+  + V PD VVGHS+GEVAAAYASG L+L +A  +VYHR+  QQ++AG GR++A+GL    ++ ++  +           A VEIAC N+P + V+   E  L+  V EL   G Q  ++PGNIAFHS+ ++P+   + A L+FLD+      +P VS+VTG+    LD++YW  N+R+PV F  A+ET+  +D  PD+V+EI PH  L   I Q +       A +  L ++    + F EALG ++  G+ +D    Y          LP HP  +   H+   ++ ++M  + G Y  GP+ G +   D   + + +S++ +  + DH++    I P   ++E++L+A  G P+ +  +EF     +         +L  +  A +Q    +   RS  T    D+E  L  HC GKV + D   ED ++       A+  L  +   D     L            ++ E FY      V    E+   F+ +  + +D  +  +L ++  D E W    ++G +    L D  L   L        +++    AC   FLR P    +  +       F +D   +Y   +G +  +G  + YD  TG L+ ++  ++    SF+ N +  D       + WQP F  P PP
Sbjct:   42 PGRFGSAYEGLIRDEGEQRMDRSLFGVSRNEMANMDPQIRMLLGCAWESCEHAGWDLHALRNSPTGVFIGAQVASTANWRALYGTNEFSILSIDASMLANRISYHLNLMGPSIACSTACSASLTALHMGINAMRQGDCDQVLVGSATYLGSARCSASFNALGVISPDGRCHSFDAD-ANGYMRAEGAFVFAVKPLDAAERDGDPIFAVIEATAVNTAGAADDAAGMAQGRYITAPTRHSQVDLMRTACARAGLRPEDFDYIEAHATGTVVGDRIEGNAIAEAFGGVE--RDVPLRVASVKSNVGHLEAAAFSAALLKVVLMMQHRTFAPISKNFLAPNLEIDFESCPMQVQTACEPFPDH-PVVVGINSFGFGGANGHCVVREYRPERP----------RVWSVPL--APGAGYMVPLSARTVNSLVESARTLREVLDER--ETDLYTLAGNLSRRRSRFPARAAFAVRDREQLAEALDAFVQDPPEVSTVEEGERRVAMVFSGQGTQWAGCGRALYDAEPVFRRVVDAIEEEWRKHSGISLRDACFTASQEELNEVQLAQPAIFMIQCALVELISTWGVQPDCVVGHSSGEVAAAYASGALSLADATHLVYHRATLQQRVAGSGRMLAIGLDRAGVETLLEELRVPFERQNHRAAQVEIACENAPANTVVCGREPALQPVVAELERRGLQNRLIPGNIAFHSAAMDPLRDDVMASLSFLDECAFDGDVPMVSSVTGRTTRCLDAEYWWSNIRQPVHFAAAMETVV-RDYRPDLVLEIAPHGALQPLIRQCVEGAPSVPACIPTLMKDQDVCLGFQEALGALYRSGVELDFASQYPR-PEPIAHLLPGHPQERTTTHD--DLVDDEMFVQRGQYSHGPLVGHRVPCDHILFEARLSEQDFPWLTDHRVHRAPIIPAAGYIELLLQAFAGDPVHIDEIEFLQHTPVGKTPVRLQTALFPVPNAPDQFTF-TISTRSFET----DDEGTL--HCRGKVRRVD---EDHTVN------AFPRLQDVLASDFDPSPL------------ATGEEFYDHVEAVVGDAFEFGPEFRSIRQIDIDSGSTDLLLEIEVDEELWASGREEGYLLYPPLTDGGLQIFLRYLMRLPDFFSMPQRACDITFLRPPTGPRISCFLVDTGDWFDVDERGQYNKPLG-ELYIGRLSFYDRETGELLAHIGEYH----SFNSNPRWSDVPDSKHLIRWQPKFVVPTPP 1218          

HSP 2 Score: 409.838 bits (1052), Expect = 3.806e-111
Identity = 292/874 (33.41%), Postives = 450/874 (51.49%), Query Frame = 0

Query: 1679 EEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPA----QGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASV----KMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGL--VAGMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGA---VAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDKKGGET 2539
            EE P     + +  F G       G+ A  + +   Q L          R         L V+T+GA  G   P      G V+ +A  L  E   +  + VD+ +   +D+  L+ ++  + RE+   + +G   V R  ++ +   RA  V   D    Y   + +   V G    T +L  + P   ++V++++ AAALNFRDVM+ L LLP  +YERS  GR +G+EASGVV   G DV+ LKVGD V      C ANR+            RV  E+AAS  SV  T++++LI+LAR+R+G+ VL+HSA GG+G AAI++A+HVGA I+ATAG + KR+ +  MGV   ++S +  W+DDLM  T+G+GVDVVLNSLAG H  L ++ALR SG   EIGK+DI+ +N L +  FRKN+ F AID+DRL LDDP  +  + +        G     P+  FP  +   AL  M TG+H GK+VL    +    G        +   +     +ATYLVTGG GGFG + V Y    GAR   +  R     +  G  R I   + +    ++E++  D  +E D ++      G+S P+K +FH+AG   D    +  P++   V   KA GA +LH  T +  ++ FV+ SSIAS  G LG  +Y+ ++++LD L   RR +GLPG ++N+  +++VG+ A +L   +     G+  +++  A+  L+  +  +   + ++T  F     T+    SP     G  +   A   V A GD +T + +   +S ++ E+ G  E  VT  L + GL S    ELG  ++  F  +VS++++    +   +++ I       + D+  GET
Sbjct: 1447 EEWPGAAELEAIDLFVGTDPEDPTGERAVWQFVAFLQAL-------APFRLESATRECRLTVVTRGAACGVDNPRGSALWGAVRSIALELLAEDTRVDFRLVDIGAE--EDLETLAAILRGNLRERELAIRDGRLWVPRIVSVRQ---RAPLVPGGDDP-PYRLRLDNPGQVSGLQMTTCELPLLGP---SDVEVEVAAAALNFRDVMVTLGLLPAMAYERSALGREVGIEASGVVRRAGSDVQRLKVGDEVALTVGGCIANRVVVNEHLAFLKPSRVSMEEAASALSVCVTAYYSLIHLARLREGQRVLVHSAMGGVGQAAIALARHVGAEIYATAGNESKREQLLAMGVRGAFDSHSHSWYDDLMEATDGEGVDVVLNSLAGHHIALCLKALRPSGWHCEIGKVDIYTDNSLSMRVFRKNLRFAAIDVDRLMLDDPVLSHMLSQACVDLLDAGAVAPPPLTVFPYRDYARALRLMTTGQHQGKLVLQAPEDAMDRG------FGIADTRPFLDPEATYLVTGGLGGFGLRFVPYLIAAGARHLTLMDRDPARSRDVGWIRQITTLSKMTQEYEIEIVPGDVRNEEDARRCI---AGLSRPLKGVFHLAGTLEDRSFLDTSPDSLARVFAPKAQGALNLHHATAEHDLDYFVLFSSIASTFGNLGQINYSGASSFLDGLAAYRRKRGLPGFSYNLGPVTEVGMAARSLHVMRMMRAAGMTTVSANFAIANLDFAMRTMTDQDHLVTALFSTPPWTV---DSPDYMRNGRLVNNQAAFEVDAGGD-LTLEGVVAQISAKVAELCGHDEGDVTEPLSSFGLTSISVAELGAFVQTQFNHQVSALELMTTASALSLAQAIVHGADSHTEDEAPGET 2291          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|460501349|gb|AGH13590.1| (type I polyketide synthase [bacterium symbiont of Plakortis simplex pPSA11D7])

HSP 1 Score: 512.301 bits (1318), Expect = 4.289e-143
Identity = 397/1282 (30.97%), Postives = 613/1282 (47.82%), Query Frame = 0

Query:    3 LDEPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGK--VSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAY---TAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALE-GPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLP-SAGVTCPRKDFEWVKHNMVIQQECEPFPDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSG---EGRKMQVAYVFTGQGSQWPGVGKTLM-AFPVYRKAVKAADKLFKSKSGWSILEKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDII----------RGMA-DVEIACVNSPESVVLASSEERLKEV-GELLPEGTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDG-AYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGT--HCVEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAW---EKKGGIFGVQLLDALLHQTLILATHSAIYYAGGFDA--CHFLRQPATKELYVYFTLDKYRLQIGRKKKV---------GDFALYDG-TGRLILYMQGFYTVFASFSDNYKLCD-------LLWQP 1236
            + + +A++G S  +P  I S + F+ ++ E+  V    V   RY        +   P +  S Y  +  +     +D  LF +     ++ + H++ L  L   +E  E AG  LH +  +RTG+FV A    T+   A  G +E  +    ++ LA+++SY     GPS+   +ACS+SL A+  A+ +L+ GDC+ A V   NYL          A GV+      H FD D A GY+R EG     ++ +A AE+ GD I   V      +AG AD ++    GR    P  +   ++     + A     +  Y+E HATGT VGD IE  AIG+  G +   R  PLR++ VKSN+GH E AA   +++K+V MM+ RTF+P S     P  + ++  H M +Q ECEPFPD+ PV VG+NSFG GG+ GH V++EY  PE  R         +W++P+  A   G + PLSA +   L   A  + + +  + P   L  L   +   R+ F  R +F      +L + L AFA   N EP     EGR+ ++  VFTGQG+QW G G  L  A PV+R+AV A D+ ++  +  S+          E+ +   AQP++F++Q  L EL K + V+PD VVGHS+GEVAA YASG L+L EA  +VYHR+  QQ+ AG GR++ +G+    ++ ++           G+A  VEIAC N+P S V+  +EE L+ V  EL     Q  ++PGNIAFHS+ ++P+       LAFL+       +P+VS+VTG+   +LD+ YW  N+RR V F  A+ET   +D  PD+V+E+ PH  L S I Q L   G  A  +  L+R+    + F EALG +F  G  +D    Y        + LP +P  ++   +     +  ++ G Y  GP+ G +   D   + + +S+R +  + +H++   +I P   ++E++L+A  GAP+ + ++EF     IP         L  +  A    G  +F + S      +D +     H  G ++   L++ D  +              L L D+          R     +    +FY      +    +Y   F+ ++ V  +  T + L  +  D   W    ++G +    LLD  L   L     +   +A    A    FLR P    L  Y   D     I  + +          G    YDG TG L+L +      + SF+ N    D       + WQP
Sbjct:    1 MGDAVAIVGYSHRMPGGIISDDDFWRVLSEREIVREPIV--DRYERGFRPIGEFSGPGRFASPYEGLIRDGREKLFDRSLFGMSHNEMLTADPHVRML--LTCTWETSERAGWDLHALRNSRTGVFVGAQVPATSNWRAPLGANEFSIASISLAMLANRISYHFNLMGPSLACCSACSASLTALHEALNALRAGDCEQAFVGSANYLGSSRQSAGFNALGVISPEGKCHSFDAD-ADGYLRSEGAFVFAIKPLAAAERDGDPIHAVVEATAVNAAGAADGSVGLAQGRYITAPTRHAQVDLMREARERAGRAPEEFDYVEAHATGTVVGDRIEGNAIGEALGSAD--RATPLRVSGVKSNVGHMEAAAFACALLKVVLMMQRRTFVPVSKNHLVPNPEIDFDAHGMQVQTECEPFPDH-PVVVGINSFGFGGSNGHCVVREYR-PEQAR---------VWSVPL--APRAGYLVPLSARTAGALVESARGLRQALDGQSP--DLYTLAGNLSRRRTHFAARTAFAVHDVDQLRQALDAFAE--NPEPVATVEEGRR-RLLMVFTGQGTQWAGCGSALYDAHPVFRRAVDAIDEHWREHADHSLRAAAFAAPQAELNECRLAQPVTFMLQCALVELFKTWGVYPDCVVGHSSGEVAAVYASGALSLAEATRLVYHRATLQQRTAGSGRMLPIGVDLAGVERLLDELDLRFRAENGVAPPVEIACENAPASTVICGTEEALRPVLEELERRNLQHRLLPGNIAFHSAAMDPLEEEAHEALAFLNDGAVDAAVPFVSSVTGEHTERLDNAYWWTNIRRRVRFAAAMETAV-RDFRPDVVLELAPHSALQSSIVQCLEDAGSRAVCIPTLRRDTDVCLGFHEALGALFRAGAELDFAAQYPR-PEPIAQLLPGYPRDEQTTADEMSDDEMFLQAGEYAHGPLVGHRVPCDHLLFEARLSERDFPWLAEHRVHHASIMPAAGYIELLLQAFGGAPLHVESLEFLQPCPIPKTPVRLQTELFPVTGAP---GRYTFTISS----RSYDVDARSELHSRGTLL---LVSADHPVD-----------VPLRLADVD-------TSRFQPYYYVGESDFYERIDASLGETFQYGPYFRNIQRVLWEDTTANYLFDVEMDERLWADGREEGYVANPALLDGGLQIFLYHLLRATDIFAMPRRAVGVTFLRPPTGPRLTCYVKKDPDWADINEQGQFTERRGERSGGSIRFYDGDTGDLVLCID----AYVSFNSNPSWNDRPHSKHVVSWQP 1223          

HSP 2 Score: 427.557 bits (1098), Expect = 1.128e-116
Identity = 302/915 (33.01%), Postives = 463/915 (50.60%), Query Frame = 0

Query: 1636 LVGESGQLTDESTADDYAALLAKVLAPQTGEELPEGD---DIAHEEEEIPNGTPFKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCPAQGTVQGLATVLCGELPDMVT-------KHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLI------TTRSAD-TEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVA--GMNPIITMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDF--------MTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKII 2523
            ++GE G L DE  A          +A     ++P  +   D   E  + P     + + FF G       G+    RL+   + L+         R +    P  L V+T+ A +G   P  G V G    +  E+            + VDL +    D+  L+ L   D RE+   V        R  ++ +         A     ++   + +   V G    TY+      P  ++V+I++ AAALNFRDVM+ L LLP  S+ERS  GR +GLEASGVV   G  V+   VGD VM  +  C ANR       V +    +  E  AS+ SVY T+++ALI+LAR+RKG+ VLIHSA GG+G AAI++A H GA I+ATAG++ KR+ +  +GV   ++S ++ W+D+L+  T G+GVDVVLNSLAG+H  L ++ALR  G   EIGK+DI+ +N L L  FRKN+ F AID+DRL  DDP  T E+ ++      +G    LPI T+P  + +EAL  M TG+H GK+VL     D + G      + V   +     +ATYLVTG  GGFG  L+ Y    GAR   +        RSAD   K   LF    D   ++++++  D  SEAD+++      G+  P+K +FH+AGV  D +L ++ PE+   V   KA GA +LH+ T    +++FV+ SSIAS +G  G  +Y+++N ++D L   RR QGLP  ++N++++++ G+ A NL   +     GV  +++  A+  L+  + A    + ++T  F          + PW+    + L  G V +  D         +T   +   ++ ++ E+ G  E      L + GL S    ELG  I+  F  +VS++++    T   +++ I
Sbjct: 1423 VIGEPGSLADEWLAG---------VARSDASDIPWAELAEDRVAELADWPGAADVQAIDFFCGQDPDDPTGEHLAGRLVAFVKALIP-------YRVAHALGPCRLTVVTQRAVLGVAEPRAGAVWGALRAISQEVAAEAGAAARLDFRLVDLKTGG--DLDTLAWLGACDLRERELAVRGNRLWAPRLVSIRERFPHLPAATAAGEDAAFRLSLDNPGQVGGLQMKTYEPAS---PGPHDVEIEVAAAALNFRDVMVTLGLLPSSSFERSALGRTVGLEASGVVRRAGDAVRTCGVGDAVMFTQGGCIANRATVKDYLVFRKPAALSMEQGASVLSVYVTAYYALIHLARMRKGQRVLIHSAMGGVGQAAIALAGHAGAEIYATAGSEGKREQLHALGVRAAFDSHSLDWYDELLAATGGEGVDVVLNSLAGRHIALCLEALRPGGWHCEIGKVDIYADNALGLSVFRKNLRFAAIDVDRLMNDDPHLTRELSQQCLNLLEKGAVPPLPITTYPYRDYEEALRLMTTGRHTGKLVLRATPADGRDGDD---RLPVADLRPYLDPEATYLVTGAFGGFGRLLLPYLATAGARHITLLDRDPQRRRSADWLRKTSALFYLPED---IEIDIVAGDVASEADVRRCI---AGLKRPLKGVFHLAGVLDDRLLADLAPESLATVFAPKAGGALNLHRATAGCELDHFVLFSSIASTLGNPGQINYSAANGFVDGLAALRRRQGLPALSYNLAAVAEAGMAARNLHVLRMSRAGGVPPVSADFAITNLDYAMRALQDSDHLVTALFS---------RPPWTVDSPDYLRTGRVLSNQDAFAVDTGGQLTQHAVVAQIAAKVAELCGHDEGSPEEPLSSFGLTSISVAELGAFIRMQFNHQVSALELMTTATCLSLAEAI 2298          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|493690238|ref|WP_006640280.1| (polyketide synthase [Bacillus sonorensis] >gi|452972754|gb|EME72582.1| polyketide synthase PksE [Bacillus sonorensis L12] >gi|1208645381|gb|ASB89465.1| 6-methylsalicylic acid synthase [Bacillus sonorensis])

HSP 1 Score: 473.781 bits (1218), Expect = 1.831e-132
Identity = 338/1038 (32.56%), Postives = 511/1038 (49.23%), Query Frame = 0

Query:    5 EPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTA--FLPACDGPDETLLR-----GQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGP-GRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFG-DSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPD-NEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQ----LFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEP----------------SGEGRKMQVAYVFTGQGSQWPGVGKTLMAF-PVYRKAVKAADKLFKSKSGWSIL-EKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMAD-VEIACVNSPESVVLASSEERLKEVGELLPE-GTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLD--SDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRL-------------HELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKG------APITLTNVEFKSMLKIPSASS 987
            EPIA+IG     P  +NS E +++++ E    ++  + + R+      D D     K  SK+      E+   +D   F +  + + ++  QQ  +L +++E LED G  L  +  + TG+F+  +T    L      + +L+      G +M+ L++++SY     GPS++++TACSSSLVA+ +A  SL   +   AL  G+N + + D+ ++    G++     S  FD   A GY+RGEG+G  VL+ ++ A K GDRI   + RA A +     N L  P G   E        E+  + Y  A +  G++ YME H TGT VGD IE  A+G V   D  E   +   I SVK+N GH E AAGI  +IK    +KH+   P   +  P     + +  + + +   P+PD N P   GVNSFG GG   HVV++E     P               PV     K  VFP SA S+  L+     + DR+ +   +     +L DLC T  + R+    R + VA + +EL  +L AF S    EP                 G    + + +V++G G QW G+G+ L+   PV+R+AV+  D+L +   GWS+L E +       + +T +AQP  F +QVGL  L K + + PD +VGHSAGEVAAAYASG L++E+A  V+YHRS  QQK  G G+L AVGLS E ++ ++ G  D V +A VNSP S+ L    + L+++ + L E G     + G + +HS  + P+   +   L   D  P     P  S VTGK  +  D  +DYW +NVR+PV F  +IE I   D      +EI PH  L   I++ L    K   +L  L+R +  S++  E LG ++  G  +D   +Y D G AF E LP +P+   R              H++HP+L      G  +  P+          + SEV+      + DH + G  +FPG  + EM L A K       + +T+ +VEF   L IP + +
Sbjct:    9 EPIAIIGIGCRFPGGVNSPESYWKMLSEGID-AISEIPSDRWGVESYYDQDRFKKGKTVSKWGGFL--EKFDQFDVEFFGMSPREAVSLDPQQRLLLMASWEALEDGGQVLERLAGSDTGVFIGGFTLDYKLLQFSESNRSLVEAHTATGSMMTLLSNRLSYAFDFRGPSISVDTACSSSLVAVHLACQSLWNHEASLALAGGVNVMLKPDYTIAESKAGMLSPDGRSKTFDSS-ANGYVRGEGVGVAVLKPLSKALKDGDRIYATI-RATAVNQDGHSNGLTVPRGESQE--------ELLRQAYSKAGISPGRVQYMEAHGTGTPVGDPIEANALGSVLSMDRPE--GDTCYIGSVKTNFGHTEAAAGIAGLIKAALCLKHKAIPPHLHLNDPNPKILFEQLCLQVPRALTPWPDTNGPAVAGVNSFGFGGTNAHVVLEEAPHVMP---------------PVRTFTEKPYVFPASARSLEALKDVAAAYKDRLEQ---DDSACSTLHDLCYTAGVKRTHHSCRLAAVADSKEELINQLHAFLSE---EPYPGLSVVQEVRHHDLVKGATASLPLVFVYSGMGPQWWGMGRKLLETEPVFRQAVEQCDRLLQQYGGWSVLKEMLKNEEQSLLEETEFAQPAIFALQVGLTALWKSWGIKPDAIVGHSAGEVAAAYASGALSIEQAVRVIYHRSRLQQKTTGMGKLAAVGLSLEDVKPLLNGFEDSVSVAAVNSPGSLTLVGDPDSLEKIIKPLEEQGVFCRYLRGKVPYHSHYMNPLKEELLKSLH--DLSPHRTITPLYSTVTGKQIAGRDLTADYWWENVRKPVQFASSIEEIIQSDY--HTFLEISPHPVLAHSISETLKNANKKGTVLASLRRFEDDSLKLFETLGGLYTAGYQIDWSIFYPD-GGAF-EKLPTYPWQLERYWQESEASAQDRLGHKVHPLL------GKRLSSPLP--------TWESEVNMTHLSYLKDHLIQGNVVFPGAAYAEMGLAAAKEIYQDSVSILTVDHVEFHKALFIPESET 990          

HSP 2 Score: 108.612 bits (270), Expect = 1.547e-19
Identity = 98/369 (26.56%), Postives = 162/369 (43.90%), Query Frame = 0

Query: 2187 IFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVD------------TGSEADMKKLTDRAHGIS----PPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRAL--------QELEMGLVAGMN-PII--------------TMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLV------TLGLDSFGFVELGGRIKKHFGIEVSSVKM 2510
            +F  D +YL+TGG G  G  + R+    GAR  ++  R    E  R  + DI  D  +   V  V                  D + L+D          P ++ + H AG ++  +L +++ ++F  V   K +GAW+LHK   D P++ FV+ SSIASL+   G  SY+++NA++D L   RR++GLP  + N       G +A  L   ++ +  G   +T+ + L        Q++    V G + P++               +  +EKT    P  +  +   G+ LT  A+ A  D    +    F+   +++I      L  S+L         GLDS   +EL  RI  HF I ++ V +
Sbjct: 1445 VFRFDGSYLITGGFGALGLLVARWLAERGARHLILMGRKPLPE--RHAWNDIRPDHPMADRVKAVKELEKLGAAVHVAAVDVNDEQSLSDYLKAYKQESWPEIRGVIHSAGAALPQILLQVQQKDFSRVMEPKTIGAWNLHKQFEDHPLDFFVLFSSIASLVASTGQGSYSAANAFMDGLAHYRRAKGLPALSINWGPWGQAG-MATQLDLLQYFVHRGFYPMTNDQGLEAFGHLLGQKVAQAAVVGADWPLVVKANFGGNQPSMLSVVTAEEKTADKDPDAA--AGQSGDPLT--ALLALKDPDARRS---FIISHLQDIAAGVLSLNRSKLSPEQSLGNYGLDSMMAIELKNRIDDHFHINIAIVDL 1803          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|657607720|ref|WP_029418910.1| (polyketide synthase [Bacillus sonorensis])

HSP 1 Score: 473.781 bits (1218), Expect = 2.039e-132
Identity = 338/1037 (32.59%), Postives = 511/1037 (49.28%), Query Frame = 0

Query:    5 EPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTA--FLPACDGPDETLLR-----GQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFG-DSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPFPD-NEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQ----LFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEP----------------SGEGRKMQVAYVFTGQGSQWPGVGKTLMAF-PVYRKAVKAADKLFKSKSGWSIL-EKIDTLSADEMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMAD-VEIACVNSPESVVLASSEERLKEVGELLPE-GTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKLD--SDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRL-------------HELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKG------APITLTNVEFKSMLKIPSASS 987
            EPIA+IG     P  +NS E +++++ E    ++  + + R+      D D     K  SK+      E+   +D   F +  + + ++  QQ  +L +++E LED G  L  +  + TG+F+  +T    L      + +L+      G +M+ L++++SY     GPS++++TACSSSLVA+ +A  SL   +   AL  G+N + + D+ ++    G++     S  FD   A GY+RGEG+G  VL+ ++ A K GDRI   + RA A +     N L  P R   Q       E+  + Y  A +  G++ YME H TGT VGD IE  A+G V   D  E   +   I SVK+N GH E AAGI  +IK    +KH+   P   +  P     + +  + + +   P+PD N P   GVNSFG GG   HVV++E     P               PV     K  VFP SA S+  L+     + DR+ +   +     +L DLC T  + R+    R + VA + +EL  +L AF S    EP                 G    + + +V++G G QW G+G+ L+   PV+R+AV+  D+L +   GWS+L E +       + +T +AQP  F +QVGL  L K + + PD +VGHSAGEVAAAYASG L++E+A  V+YHRS  QQK  G G+L AVGLS E ++ ++ G  D V +A VNSP S+ L    + L+++ + L E G     + G + +HS  + P+   +   L   D  P     P  S VTGK  +  D  +DYW +NVR+PV F  +IE I   D      +EI PH  L   I++ L    K   +L  L+R +  S++  E LG ++  G  +D   +Y D G AF E LP +P+   R              H++HP+L      G  +  P+          + SEV+      + DH + G  +FPG  + EM L A K       + +T+ +VEF   L IP + +
Sbjct:    9 EPIAIIGIGCRFPGGVNSPESYWKMLSEGID-AISEIPSDRWGVESYYDQDRFKKGKTVSKWGGFL--EKFDQFDVEFFGMSPREAVSLDPQQRLLLMASWEALEDGGQVLERLAGSDTGVFIGGFTLDYKLLQFSESNRSLVEAHTATGSMMTLLSNRLSYAFDFRGPSISVDTACSSSLVAVHLACQSLWNHEASLALAGGVNVMLKPDYTIAESKAGMLSPDGRSKTFDSS-ANGYVRGEGVGVAVLKPLSKALKDGDRIYATI-RATAVNQDGHSNGLTVP-RGESQ------EELLRQAYSKAGISPGRVQYMEAHGTGTPVGDPIEANALGSVLSMDRPE--GDTCYIGSVKTNFGHTEAAAGIAGLIKAALCLKHKAIPPHLHLNDPNPKILFEQLCLQVPRALTPWPDTNGPAVAGVNSFGFGGTNAHVVLEEAPHVMP---------------PVRTFTEKPYVFPASARSLEALKDVAAAYKDRLEQ---DDSACSTLHDLCYTAGVKRTHHSCRLAAVADSKEELINQLHAFLSE---EPYPGLSVVQEVRHHDLVKGATASLPLVFVYSGMGPQWWGMGRKLLETEPVFRQAVEQCDRLLQQYGGWSVLKEMLKNEEQSLLEETEFAQPAIFALQVGLTALWKSWGIKPDAIVGHSAGEVAAAYASGALSIEQAVRVIYHRSRLQQKTTGMGKLAAVGLSLEDVKPLLNGFEDSVSVAAVNSPGSLTLVGDPDSLEKIIKPLEEQGVFCRYLRGKVPYHSHYMNPLKEELLKSLH--DLSPHRTITPLYSTVTGKQIAGRDLTADYWWENVRKPVQFASSIEEIIQSDY--HTFLEISPHPVLAHSISETLKNANKKGTVLASLRRFEDDSLKLFETLGGLYTAGYQIDWSIFYPD-GGAF-EKLPTYPWQLERYWQESEASAQDRLGHKVHPLL------GKRLSSPLP--------TWESEVNMTHLSYLKDHLIQGSVVFPGAAYAEMGLAAAKEIYQDSVSILTVDHVEFHKALFIPESET 990          

HSP 2 Score: 108.997 bits (271), Expect = 1.458e-19
Identity = 98/369 (26.56%), Postives = 162/369 (43.90%), Query Frame = 0

Query: 2187 IFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVD------------TGSEADMKKLTDRAHGIS----PPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRAL--------QELEMGLVAGMN-PII--------------TMFFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLV------TLGLDSFGFVELGGRIKKHFGIEVSSVKM 2510
            +F  D +YL+TGG G  G  + R+    GAR  ++  R    E  R  + DI  D  +   V  V                  D + L+D          P ++ + H AG ++  +L +++ ++F  V   K +GAW+LHK   D P++ FV+ SSIASL+   G  SY+++NA++D L   RR++GLP  + N       G +A  L   ++ +  G   +T+ + L        Q++    V G + P++               +  +EKT    P  +  +   G+ LT  A+ A  D    +    F+   +++I      L  S+L         GLDS   +EL  RI  HF I ++ V +
Sbjct: 1445 VFRFDGSYLITGGFGALGLLVARWLAERGARHLILMGRKPLPE--RHAWNDIRPDHPMADRVKAVKELEKLGAAVHVAAVDVNDEQSLSDYLKAYKQESWPEIRGVIHSAGAALPQILLQVQQKDFSRVMEPKTIGAWNLHKQFEDHPLDFFVLFSSIASLVASTGQGSYSAANAFMDGLAHYRRAKGLPALSINWGPWGQAG-MATQLDLLQYFVHRGFYPMTNDQGLEAFGHLLGQKVAQAAVVGADWPLVVKANFGGNQPSMLSVVTAEEKTADKDPDAA--AGQSGDPLT--ALLALKDPDARRS---FIISHLQDIAAGVLSLNRSKLSPEQSLGNYGLDSMMAIELKNRIDDHFHINIAIVDL 1803          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|922862950|gb|KOO30472.1| (beta-ketoacyl synthase [Chrysochromulina sp. CCMP291])

HSP 1 Score: 468.003 bits (1203), Expect = 1.470e-128
Identity = 395/1322 (29.88%), Postives = 618/1322 (46.75%), Query Frame = 0

Query:    6 PIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIF----VAAYTAFLPACDGPDETLLRGQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLP-SAGVTCPRKDFEWVKHNMVIQQECEPFPD-NEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVF--PLSAVSMPHLQLFADRMAKYIKERGPTIS---LKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAF---ASSGNTEPS-----------------------GEGRKMQVAYVFTGQGSQWPGVGKTLMAF-PVYRKAVKAADKLFKSKSGWSILEKIDTLSADE-----MRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGMA-----------DVEIACVNSPESVVLASSEERLKEVGE-LLPEGTQKA-MVPGNIAFHSSRVEPILPAMKARLAFLDKRP---KTWGLPYVSAVTGKLESKLDSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCP-LKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPFIKRRLHELHPVLKN--DMRNGLYIDGPVAGVQKFFDGA---YVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVKGAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKWDEEPVLVEHCTGKVVKADLLNEDGSIKDGFKPEAYGLLAGLELKDIGDGGLKDLIGRHTQCEHSSTENFYGTHC--VEGQMEYLESFQLVESVHVDPKTMSILGKLNYDNEAWEK---KGGIFGVQLLDALLHQTLILATHSAIYYAG---GFDACHFLRQPATKELYVYFTLDKYRL----QIGR------KKKVGDFALYDG-TGRLILYMQGFYTVFASFSDNYKLCDLL-------WQP 1236
            PIA+IG    LP  IN+ + F+++I E+    V      RY            P ++ S Y  +  + E   +D  LF +    ++ M  Q   +L   +E LE AG+    ++ + TG+F    V++   + P   G   + + G+ +S +++++S+     GPS ++ TACSS + A+  A+ SL  G+CD A    +NYL  +   +     G++ +  TS  FD+D A GY+R EG    +L+R+ DAE+ GDRI   +      +AG   +A  G          +  + +   + + A +    + Y+E HATGTAVGD IE  AI   +G     R   LR+AS +SN+GH E A+   S++KI+ M++ R F P SA    P     W    M +Q   EPFP  +EP+ +G+NSFG GGA GH ++QEY  P            + W+   E       VF  PL A S   L   A ++A +++   P  S   + +LC  +  HR     R +F A+T  EL   L AF   AS    +P                        G      V +VF GQGSQW G GK L    PV+++ V   DK +   +G +   K+  ++ DE     + +  +AQP++FL+Q GL+E+LK   V PD VVGHSAGE  +AYASG  TLEEA  +VY R+  QQ  AGCGR++ + +    ++  +  +             V++AC NSP + V+  +   + ++ E L+ +G Q   ++PGNIAFHS  + PI   +   L  LD      +T  +P +S VTG++   +D++YW  NVRRPV     I T+      PD+++EI PH TL SPI +       +     P L R    +  F  ALG  F+ GL VD +  +          LP++P  K R+  +  ++ N   +R   +  GP+ G  K   G    Y +  S   +  M DH +   AI P   ++E++LEA +G P+ + +  F +   + +     + +L  +  A  +     F + S P     D    +  HC G + K +L     +IK G    A  L   + L         D  G+ ++C   + E FY      V     Y   F++++ V  D +T+++  ++  +   W+K    G +    +LD +L Q  I+    A  ++G     +   F+++P T++L   +    Y      Q G+      ++  G   +YDG TG L+ Y+ G YT   SF  N K  DL        WQP
Sbjct:    8 PIAIIGYGYRLPGGINNDDEFWKLISERG--FVQEPIEDRYGKGQCPHDGFDSPMRVASPYEGLIKDGEELHFDCALFGMSPNDAKRMDPQCKMMLSCTWEALEMAGLDQASLHNSNTGVFCGVQVSSCAGWRPPY-GATPSDVPGKSLSMISNRISFHFNWMGPSFSVATACSSGITALDAAIKSLSLGECDLAASGAVNYLGHEVGSIGFNQLGIISKTGTSRSFDKD-ANGYMRAEGAFMYLLKRLPDAERDGDRIFAVIRGCGLNTAGAEKDADVG----------HAQQSLMELIARQAGIDPLAVDYVEAHATGTAVGDAIEGNAIARAYGSP--ARTTLLRLASTRSNVGHMEAASFTCSLLKILLMLQKRHFAPVSAHHKSPNPKIPWEGQRMKVQTSAEPFPQRDEPILIGINSFGFGGANGHCIVQEYKAP------------ATWSSVPEAIHLGRKVFSVPLCAKSDEALVDRARQLAAFLQSASPEDSYTLIGNLCCRVTPHR----VRTAFSAATLPELQAALEAFVAKASKNELKPEDVVRVQLKSRLSSQELIPAGVAEGSVTPPTVLFVFPGQGSQWAGCGKRLYETEPVFKRVVDTIDKTWSQCAG-ADTPKLSGVAFDENFGKELNECKWAQPVTFLIQAGLFEMLKAHGVHPDAVVGHSAGEAGSAYASGAYTLEEATRLVYDRARLQQLTAGCGRMLVLMMERSAVEASLTKLGFDYQGEGLEGDVVDLACFNSPNNTVVCGAPATIAKLQEALVADGLQAGKLIPGNIAFHSRHMLPIKAELYKVLGQLDGPSGALRTGPIPLLSTVTGRVLDSVDANYWWSNVRRPVNLLDTIRTV-KLLLKPDVIVEISPHITLRSPIRECYEENDGTMPPYVPTLIRGVDDAAAFANALGACFKNGLLVDYRALFPR-PEPMTHKLPRYPMKKERM--IDDLIDNFAHLRMESFHRGPMLGADK--QGPIKQYGNVFSKPHFVWMADHVVQETAIIPASGYIELLLEAFRGEPVHVVHARFIAPFTLTADPKHLHTNLEPVPHAPNR---YEFTITSRP-HSSLDTASTV--HCVGVLEKLNL-----AIKGGL---ANALPTQVPL--TFPVARPDGKGKFSECMFENAETFYAAMQARVGEAFSYGPYFRVIKRVDRDVETLNLRVEIEQEATMWKKWDLMGYLLHPTVLDGVL-QIFIVYVMEAPDFSGVPQMMNDFIFVKKPTTEKLVCVYEPPAYLKGNVHQKGQLAFALGERPAGAIQIYDGVTGELVAYL-GRYT---SFHANAKKPDLRKSKHFLSWQP 1269          

HSP 2 Score: 390.578 bits (1002), Expect = 5.600e-105
Identity = 267/830 (32.17%), Postives = 426/830 (51.33%), Query Frame = 0

Query: 1740 VITKGAYVGDVCPAQGTVQGLATVLCGELPDMVT---KHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKEN--EVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDAASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEALEFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADA-----TYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFR-----DIL--DDASVKMEVMTVDTGSEADMKKLTDRAHGIS-PPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDL-PIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQELEMGLVAGM--NPIITM----FFKEKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTKEIAHFLSEEIKEITGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQS--RGSNDKKGGETADE 2542
            V T GA +    PAQ  V G    L  EL D      + VDL + +  D+  +  L     RE+   + NG    +R + + +T   A   L  D   ++     ++ ++        Q R +P P+    +V+I +  AALNFRD+M+ L  LP  SYERS  GR +G+E +G V  +G  VK  KVGD+V+  +  C ANRL        K+   V +E  +SL SVY T++++LI LAR+RKG+T+L+HSA GG+G AAI++AKH GA +  TAG+  + + +  MG    ++S    W+ D+M+ T G+GVD+VLNSLAG+H  L ++AL + G   EIGK+DI+ +  L +  FRKN+++ AID+DRL +DDP   AE+         +G    LPI  F  ++ + AL  M  G H GK+VLT    DK        ++ V   ++++         T L++G  G FG +L  Y    GA+  L+  R    ++     R     D L  D A+V++E++  D    +D+     +   +  PP+ S+FH+AG+  D  + +M   +F  V   KA GAW+LH+ T  L P+++FV++SS +S  G  G ++Y+++N+Y D L   R+  GL G ++ M ++ + G+ A N          G+  I+S  A++ L+  + +G+  N +  +    F  +   + F   +    H G    LG     G  ++ + +   +S ++  + G +EV  T  L T GL+S    ELG  +K       S++++    T E ++  I   Q     S  K  G+ AD+
Sbjct: 1477 VFTTGAVLKVTSPAQTGVWGAVRTLSLELGDACKVDFRLVDLGAIS--DLALVPHLA--PLRERELAIRNGRLWTSRLHNLRETIPIAH--LTADAPCAFRLTTDNSGAI-----ADLQFRTLPLPELGPLDVEIAVKGAALNFRDIMVGLGRLPLLSYERSALGRTVGIECAGQVVRVGTGVKRHKVGDKVLAMQGGCIANRLRCHELAAFKMPSNVSYEQGSSLLSVYVTAYYSLIYLARMRKGQTILVHSAMGGVGQAAIALAKHTGAHVIGTAGSKSRCEKLMAMGCVAAFDSHTTSWYADVMKFTQGKGVDIVLNSLAGEHVDLCLEALAAGGWHCEIGKVDIYADRPLKMAVFRKNLAYRAIDIDRLMMDDPMLAAELTESCLELIQKGKVPCLPITCFEYSQHQAALRSMMGGLHEGKIVLTPPPADK--------TIEVGDTRSLYGVGKDGKPRTVLLSGCLGDFGLRLFAYLIAMGAKHILVLDRDPKRKRTVEWMRQHAYVDYLMPDSANVRIEIVRADVAVYSDVVDALGQVKALGLPPIGSVFHLAGILDDKFVSDMDRASFARVYAPKADGAWNLHRATQKLEPLDHFVMLSSTSSAFGNPGQTNYSAANSYQDGLAAMRQQAGLAGLSYCMGAVIEAGMAARNPQLLNMMKAGGMPAISSIFAIEVLDSAIRSGVHSNAVAALASDRFSADLGSSDFLRSAAQLVHNGAVFRLGG----GGGLSKEALIERISSKVAALCGVAEVSPTDPLSTYGLNSISVAELGAFLKTEMAYSASAMQLMTSATCESIANGILAAQGGVDASATKAKGDEADK 2283          

HSP 3 Score: 72.7886 bits (177), Expect = 1.543e-8
Identity = 40/127 (31.50%), Postives = 64/127 (50.39%), Query Frame = 0

Query: 2566 FEEDDDAANNHKAFHLRPLTSSAK----LLFGATGLTGSHLLRYMIK---AGVPKVFCIVRGSTSEGGLARIEKTMRLLKIWKPSFAANIVVIPGDMALTNFGIKPDMYARLVHEVDAVMHAGGSRK 2685
             +E +   N+ +   L+P+T   +    LL GATG  G H + Y+++     + K++C VR S+ E  LAR+ + M +  +WK +    + V  GD+   NFG  P +Y  L   VD V H   + K
Sbjct: 2376 LDELNGFVNSFETSGLQPVTPVQQVRRVLLTGATGFLGRHFIPYLLERKDVTIEKIYCPVRASSDEKALARVIEAMTMCGLWKDALRERLCVFAGDLHDINFGASPTLYQELSASVDGVYHFASNLK 2502          

BLAST of EWM29221.1 vs. NCBI_GenBank
Match: gi|953252307|emb|CUS36179.1| (conserved hypothetical protein [Candidatus Nitrospira nitrosa])

HSP 1 Score: 459.144 bits (1180), Expect = 7.305e-127
Identity = 322/1051 (30.64%), Postives = 513/1051 (48.81%), Query Frame = 0

Query:    5 EPIAVIGASIVLPANINSRERFFEIIREKNQVSVDTVAAGRYHASDIDDSDSGHPWKLKSKYSMIFTEEELGSYDSHLFNIQGKVSRNMHLQQLGVLQSAFEGLEDAGIPLHDIYRTRTGIFVAAYTA--FLPACDGPDETLLR-----GQIMSSLADQVSYFLGTYGPSVTLETACSSSLVAIAMAVASLQQGDCDTALVTGLNYLNEKDFHLSLQACGVMVQGPTSHPFDEDGAKGYIRGEGMGTVVLRRMADAEKHGDRILCKVLRAIAASAGPADNALEGPGRVYEQPCPYGMREMFSRVYKCANVPLGKLHYMECHATGTAVGDVIELQAIGDVFGDSHELRKNPLRIASVKSNIGHAEVAAGIFSVIKIVQMMKHRTFLPSAGVTCPRKDFEWVKHNMVIQQECEPF-PDNEPVTVGVNSFGIGGAYGHVVMQEYTGPEPVRRALLTSASSLWALPVEDAAAKGLVFPLSAVSMPHLQLFADRMAKYIKERGPTISLKDLCATMWIHRSRFQYRKSFVASTTQELAEKLAAFASSGNTEPSGEGRKMQVA-----YVFTGQGSQWPGVGKTLM-AFPVYRKAVKAADKLFKSKSGWSILEKIDTLSAD-EMRDTLYAQPISFLVQVGLYELLKFFKVFPDVVVGHSAGEVAAAYASGLLTLEEACEVVYHRSAEQQKMAGCGRLMAVGLSEEKIQDIIRGM-ADVEIACVNSPESVVLASSEERLKEVGELLPE-GTQKAMVPGNIAFHSSRVEPILPAMKARLAFLDKRPKTWGLPYVSAVTGKLESKL--DSDYWVDNVRRPVLFQKAIETIFSQDTGPDIVIEIGPHKTLCSPIAQVLSAMGKSAAILCPLKRNDTCSMRFLEALGQMFEKGLSVDCKQWYQDLGYAFDEDLPKHPF-------------IKRRLHELHPVLKNDMRNGLYIDGPVAGVQKFFDGAYVSEVSDRTYKQMCDHKMGGQAIFPGMFFVEMVLEAVK----GAPITLTNVEFKSMLKIPSASSGENPSLIALNFAEEQDGIRSFVVRSCPTREKW 1019
            EPIA++G     P  +     F+E+++      VD V + R++     D D+  P K+  K    F  + +  +D+  F I  + +  +  QQ  +L++++E LEDAGIP   +  + TG+F+ A+T    L      +  L+      G  M+ L++++SY L   GPS++L+TACSSSLVA+ +A  ++ +G+CD AL  G+N +   ++ +++   G +     S  FD   A GY RGEG G VVL+R+++A + GD I   ++R    +     N +  P        P     +  RV     +    + Y+E H TGT VGD +E +A+G   G  H    +P  + SVK+ IGH E AAG+ ++IK    + HR   P A +  P  D  +    + + ++ E   P  E V  GVNSFG GG   HVV+Q          A L  A  L A P++         PLSA S   L   A    +Y  ER P   L DL  +    R    +R +   ++ +E+  +L AF   G  E    G++   +     +V+TG G QW  +G+ L    PVYR AV+  D +F++ +GWSILE++     D  M DT+ AQP +F++QVGL  L +   V P  ++GHS GEV AAY +G+LTLE+A  V YHRS  Q+K AG G+++AV LS+++   +++ M  +V IA +NSP +V LA     L+ + E L + G     +   + +HS  +EP+ P +++ LA L  +P+   +P  S VTG + + +  D++YW DN+R PV F + I ++     G  + +E+GPH  L + I +        A  +  L+R       F   L Q++  G  +D ++ Y   G      LP +P+             + R    LHP+L          D  V   +  ++ A    V+ +    + DH++ G  + PG  +VE  L A +     A   L  ++F   L +P+A   +    + L+   +  G   F       ++ W
Sbjct:   15 EPIALVGIGCRFPGGVTHAHSFWELLKTGQDAIVD-VPSDRWNVRRFYDPDANKPGKMYVKQGG-FLRQRISEFDALFFGIAPREAECIDPQQRLLLETSWEALEDAGIPHGALAGSNTGVFIGAFTLDHKLTQMGKMNRQLIGTHTAIGSTMTILSNRISYVLDLRGPSMSLDTACSSSLVAVHLACQAIWRGECDLALAGGVNVMMRPEYPMAMCKGGFLAPDGRSKSFDSR-ANGYGRGEGAGVVVLKRVSEAMRDGDSIYA-LIRGTGVNQDGRTNGITVPN-------PQSQTALIQRVCTQYAIDSRDIRYVEAHGTGTPVGDPLEAKALGTALG--HPEEHDPCLLGSVKATIGHLEAAAGVAAIIKTALCLSHRQVPPQANLETPNPDIPFEALGLKLPRQLEELAPGAEQVCAGVNSFGYGGTNAHVVLQN---------APLVEADGLLAAPLDSVHC----LPLSARSEHALTALAKSWLEYFGER-PERRLSDLAYSASCRRGHHDHRLAVTGASWEEVRGQLQAFVEQGMGEWFASGKRGNESEQPPVFVYTGMGPQWWAMGQELYEKEPVYRAAVEECDAIFRNIAGWSILEEMRKPEQDSRMADTVIAQPANFVIQVGLTALWRSRGVNPGAIIGHSVGEVTAAYVAGVLTLEDAVLVSYHRSRIQKKAAGTGKMLAVNLSQDQCASLLKEMKGEVSIAAINSPTAVTLAGDALALQAIQEHLAQRGEAGRFLQVEVPYHSPFMEPLKPEVRSVLAQL--KPQKPTIPLYSTVTGDVVTGVLYDAEYWCDNIREPVYFAEGISSLLRD--GHRVFLEVGPHPVLSTSIKECCRHRNVEAQTIASLRRQKPERRTFSMGLAQLYIAGCPIDWRRQYSADGRYV--KLPTYPWQREVYWSEDAESQVDRIGESLHPLL----------DRRVPNPRPMWEAA----VNGQILPYLNDHQVDGLVVMPGAAYVEAGLAAFQQVTGDARCALVQLQFHQAL-MPNAGGADQIVHVELD---QTSGEYGFYSHESTEQQNW 1014          

HSP 2 Score: 182.185 bits (461), Expect = 7.897e-42
Identity = 222/885 (25.08%), Postives = 382/885 (43.16%), Query Frame = 0

Query: 1687 FKGVIFFAGLHDTSVIGDVAFSRLLKLAQGLLKNGDLLKTLRGSELAEPNSLWVITKGAYVGDVCP-------AQGTVQGLATVLCGELPDMVTKHVDLASSNSKDMRCLSKLVVEDSREKSYTVMNGAAQVARFNAMDKTNARAMQVLATDPKLSYFADITSAASVPGEINVTYQLRDVPPPKENEVQIDICAAALNFRDVMIALSLLPEKSYERSYYGRNLGLEASGVVSAIGRDVKDLKVGDRVMTGEPRCFANRLNAPASRVVKLDQRVPFEDA--ASLQSVYNTSHHALINLARIRKGETVLIHSAAGGIGHAAISIAKHVGAVIHATAGTDEKRQLVKDMGVEFVYNSRNVGWFDDLMRDTNGQGVDVVLNSLAGKHQRLGVQALRSSGRFLEIGKMDIFDNNGLPLLAFRKNISFFAIDMDRLALDDPAQTAEIGREVGMHFMEGHYDKLPIVTFPMNEIKEAL-----EFMKTGKHVGKVVLTNYLEDKKTGKVSPCSVTVEKPQNIFHADATYLVTGGAGGFGSKLVRYAYYNGARSFLITTRSADTEKVRGLFRDILDDASVKMEVMTVDTGSEADMKKLTDRAHGISPPVKSIFHVAGVSVDIVLPEMKPENFYEVANCKALGAWHLHKGTLDLPIENFVVVSSIASLIGGLGMSSYASSNAYLDALMRARRSQGLPGAAFNMSSLSDVGILANNLSARKFQLKMGVEYITSFRALQ------ELEMGLVAGMNPIITMFFK-EKTRTMFPFQSPWSYHLGEALTLGAVAAKGDFMTTK--------------EIAHFLSEEIKEI--TGASEVLVTSQLVTLGLDSFGFVELGGRIKKHFGIEVSSVKMSPDNTLEDVSKIIYKLQSRGSNDK 2534
              GV++  G  D +   D A      LA  +++   LL ++   E   P  L+++T+GA V  V P       AQ ++ GLA V   E P +    VD+A+ +  ++R L+  ++ D RE    + +    V R   +      A+    +D + +  AD   A  + G    T+Q   +P P+  EV + +    +N   V +      E+   R Y+       A+G V+A+G  V   K+GD+V+       A+ +  P  RV  +D    F+D+  A++ + + ++++AL  +AR+  GETVLI +A G    A   IA+ +GA  +    T E  + +    V  ++N  +   + + +R    Q +   ++ +  K   L   +L    + L      IF +            S   ID  +LAL  P    E+  +VG      H    P V+  +  ++E L     E  K G     V L     D+ + +V    VT       F  D TYL+TGG GGFG +   +    GAR   +  R     +        L+   V +  +  D   E  +++   +     PP+K +FH AG+  D  + E+ P+    V   KAL AW+LH  TL   +++FV+ SS++SLIG    +SY ++N +LDAL   R + GL G + N  +++  G+   N    K    MG+  IT+ +AL+      E ++  V  MN     + + E T    P  S          TL       + MT +               +   ++E++  +     S+V +   L  +G+DS   VEL   I + FG+ +S++++     L  +++++ +   +   +K
Sbjct: 1277 LDGVVYCWGTQDPADTDDPAGIARTGLAVQVIQ---LLTSM--FEERSPR-LYIVTQGAQV--VPPTERIHGLAQTSLVGLARVTFNEHPALRCTLVDVAAMD-PNVRALAHEILADDREDDVVLRDDGRYVHRLERL------AVDAAVSD-RRTVMADAVEAFRLHGTGRPTFQAMPIPIPQVGEVVVGL--EYINSSSVQLEAGATTEEV--RGYF-------ATGTVTAVGEAVGKWKIGDKVLIAAEGRPASHVVCPIDRVFSVDLYRGFDDSETAAVPTTFFSAYYALQAIARLIPGETVLIDAALGARAIATHRIARWLGAKPYLYT-TTEGLEGLNGEPVMLLFNKPDSASWSEGIRQL--QHIRAWVHGIHTKEHTLIANSLSPDTQEL------IFADGATRGSVMGGLGSCTIIDAVKLALTAPQLFGELFGKVGQILT--HQVGSPSVS-QIVSVEEGLSGPSRENEKRGSSEAIVSLI----DRASIEVVDDRVT-----PFFKTDGTYLITGGFGGFGLETATWLAKRGARYLALVGRRGAAAEHAKAAVQSLESQGVNVLAIAADMSDERQVEETLAQVANQFPPLKGVFHAAGILDDAPIAELNPDRIATVMKAKALSAWYLHTHTLKSQLDHFVLFSSVSSLIGNARQASYVAANTFLDALAHHRSAAGLAGVSVNWGAIA-TGMATENDDVVKHLELMGMSAITAEQALEGWAHLRENDLPQVGIMNCSWPRWREFEPTGGNAPRFS----------TLTGGGENSNSMTERCQAISELPVHERAAAMGQAVAEQVARVLRMPLSQVDLQHSLAQMGVDSLMSVELQAAIDQVFGVRISTLELMRSKNLLHLAELLMERTIQAVTNK 2102