EWM30541.1, cds128 (CDS) Nannochloropsis gaditana

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|585113205|gb|EWM30541.1| (malate synthase a [Nannochloropsis gaditana])

HSP 1 Score: 1623.99 bits (4204), Expect = 0.000e+0
Identity = 783/783 (100.00%), Postives = 783/783 (100.00%), Query Frame = 0

Query:    1 MESTFKLHPLPQVVLEALQAASSKEGCPEGMEIVGGLTEEQQSLLTLEILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIVRALHRGLAVLAKQVGEAKVRGTIKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETSLSLHVAANAAQYQDGLLRFIRMEPPSPDVKDHELIEVPADQHTPDKFTYAGLKQYLRLALFYTASFIHGTGCIALDGVFEDLATAEKARVNVWQWLTLDQPLMLSSSSSATLTPALLAQAMEEVYHAALHDERGLWGAVCKSVPPMQPDSLAEKHFRVARKVLMILMTNDRCIEFIPDLAYPFLLDPVREDDKLWAQVEEGLDAYADPMTRYVVGKKEV 783
            MESTFKLHPLPQVVLEALQAASSKEGCPEGMEIVGGLTEEQQSLLTLEILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIVRALHRGLAVLAKQVGEAKVRGTIKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETSLSLHVAANAAQYQDGLLRFIRMEPPSPDVKDHELIEVPADQHTPDKFTYAGLKQYLRLALFYTASFIHGTGCIALDGVFEDLATAEKARVNVWQWLTLDQPLMLSSSSSATLTPALLAQAMEEVYHAALHDERGLWGAVCKSVPPMQPDSLAEKHFRVARKVLMILMTNDRCIEFIPDLAYPFLLDPVREDDKLWAQVEEGLDAYADPMTRYVVGKKEV
Sbjct:    1 MESTFKLHPLPQVVLEALQAASSKEGCPEGMEIVGGLTEEQQSLLTLEILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIVRALHRGLAVLAKQVGEAKVRGTIKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETSLSLHVAANAAQYQDGLLRFIRMEPPSPDVKDHELIEVPADQHTPDKFTYAGLKQYLRLALFYTASFIHGTGCIALDGVFEDLATAEKARVNVWQWLTLDQPLMLSSSSSATLTPALLAQAMEEVYHAALHDERGLWGAVCKSVPPMQPDSLAEKHFRVARKVLMILMTNDRCIEFIPDLAYPFLLDPVREDDKLWAQVEEGLDAYADPMTRYVVGKKEV 783          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|553180839|ref|XP_005853468.1| (malate synthase [Nannochloropsis gaditana CCMP526] >gi|422295593|gb|EKU22892.1| malate synthase [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1603.57 bits (4151), Expect = 0.000e+0
Identity = 777/798 (97.37%), Postives = 779/798 (97.62%), Query Frame = 0

Query:    1 MESTFKLHPLPQVVLEALQAASSKEGCPEGMEIVGGLTE---------------EQQSLLTLEILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIVRALHRGLAVLAKQVGEAKVRGTIKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETSLSLHVAANAAQYQDGLLRFIRMEPPSPDVKDHELIEVPADQHTPDKFTYAGLKQYLRLALFYTASFIHGTGCIALDGVFEDLATAEKARVNVWQWLTLDQPLMLSSSSSATLTPALLAQAMEEVYHAALHDERGLWGAVCKSVPPMQPDSLAEKHFRVARKVLMILMTNDRCIEFIPDLAYPFLLDPVREDDKLWAQVEEGLDAYADPMTRYVVGKKEV 783
            MESTFKLHPLPQVVLEALQAASSKEGCPEG+EIVGGLTE               EQQSLLTL ILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQ+AGVLNGHGRITDLLCEPDFVLEENVTS VTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPP AIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIVRALHRGLAVLAKQVGEAKVRG IKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETSLSLHVAANAAQYQDGLLRFIRMEPPSPDVKDHELIEVPADQHTPDKFTYAGLKQYLRLALFYTASFIHGTGCIALDGVFEDLATAEKARVNVWQWLTLDQPLMLSSSSSATLTPALLAQAMEEVYHAALHDERGLWGAVCKSVPPMQPDSLAEKHFRVARKVLMILMTNDRCIEFIPDLAYPFLLDPVREDDKLWAQVEEGLDAYADPMTRYVVGKKEV
Sbjct:    1 MESTFKLHPLPQVVLEALQAASSKEGCPEGIEIVGGLTEMLMLYFLPLYSVTFKEQQSLLTLNILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQDAGVLNGHGRITDLLCEPDFVLEENVTSNVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPMAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIVRALHRGLAVLAKQVGEAKVRGAIKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETSLSLHVAANAAQYQDGLLRFIRMEPPSPDVKDHELIEVPADQHTPDKFTYAGLKQYLRLALFYTASFIHGTGCIALDGVFEDLATAEKARVNVWQWLTLDQPLMLSSSSSATLTPALLAQAMEEVYHAALHDERGLWGAVCKSVPPMQPDSLAEKHFRVARKVLMILMTNDRCIEFIPDLAYPFLLDPVREDDKLWAQVEEGLDAYADPMTRYVVGKKEV 798          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|522132946|ref|WP_020644155.1| (malate synthase A [Amycolatopsis balhimycina])

HSP 1 Score: 202.986 bits (515), Expect = 4.849e-53
Identity = 140/444 (31.53%), Postives = 196/444 (44.14%), Query Frame = 0

Query:   56 LVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            L + + TF+     L+  RR R+EE+      ++P + F  ET  IRD +        P + DR  E T P+    M ++ LNS A     D ED+ +P   NV+ G   +++A+   I                      F++ A +   +  ++     H  I D                               PATI  R RG H++E H  +DG+PVSA L+DF +F       L A    P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+    +   RRG  ++GGM   IP+ DP ++  A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:   31 LAKLDNTFAGRRRELLDARRVRREELQSG---EKP-LGFLPETSRIRDDESWHVAPTAPGLEDRRVEITGPTDR-KMTVNALNSGAKVWLADFEDATSPTWHNVIDGQLNLFDAIRRNID---------------------FTTEAVSNAASAGKSTSAGKHYTIGD------------------------------DPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLFFFHNARQLLARGVGPYFYLPKLENHLEARLWNDVFLLAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSMIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVRTCHRRGAHAIGGMAAFIPSKDPEVNATAAEKVRADKEREAGDGFDGSWVAHPGLVPICREVFDNV 396          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|760098596|ref|WP_043780840.1| (malate synthase A [Amycolatopsis rifamycinica] >gi|637498594|gb|KDN21444.1| malate synthase [Amycolatopsis rifamycinica])

HSP 1 Score: 198.749 bits (504), Expect = 1.203e-51
Identity = 141/444 (31.76%), Postives = 193/444 (43.47%), Query Frame = 0

Query:   56 LVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            L + + TF+     L+  RR R+EE+      ++P + F  ET+ IRD +        P + DR  E T P+    M ++ LNS A     D ED+ +P   N                         V+ G+L+  D                             +    DF  E     T+ D            PATI  R RG H++E H  +DG+PVSA L+DF +++      L A  A P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+    Q   +RG  ++GGM   IP+ DP I+  A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:   31 LAKLDNTFAGRRRELLDARRTRREELQSG---EKP-LGFLPETRRIRDDESWHVAPTAPGLEDRRVEITGPT-DRKMTVNALNSGAKVWLADFEDATSPTWHN-------------------------VIDGQLNLYDA----------------------------IRRNIDFTTEAGKRYTIGD-----------DPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLYIFHNARQLIARGAGPYFYLPKLENHLEARLWNDVFLLAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSLIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVQTCHKRGAHAIGGMAAFIPSKDPEINATALEKVRADKEREAGDGFDGSWVAHPGLVPVCREVFDSV 383          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|1197729600|ref|WP_086680702.1| (malate synthase A [Amycolatopsis pretoriensis] >gi|1089631364|emb|SEF37041.1| malate synthase [Amycolatopsis pretoriensis])

HSP 1 Score: 197.208 bits (500), Expect = 5.459e-51
Identity = 136/444 (30.63%), Postives = 192/444 (43.24%), Query Frame = 0

Query:   56 LVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            L + + TF+     L+  RR R+EE+      ++P + F  ET+ +R+          P + DR  E T P+    M ++ LNS A     D ED+ +P   NV+ G   +++A+   I                                                     DF  E     T+ D            PATI  R RG H++E H  +DG+PVSA L+DF ++       L A    P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+        +RG  ++GGM   IP+ DP++++ A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:   31 LAKLDNTFAGRRRELLDARRVRREELQSG---EKP-LGFLPETRRVREDKSWHVAPTAPGLEDRRVEITGPT-DRKMTVNALNSGAKVWLADFEDATSPTWHNVIDGQLNLHDAIRRNI-----------------------------------------------------DFTTEAGKRYTIGD-----------EPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLYFFHNARQLLARGVGPYFYLPKLENHLEARLWNDVFLMAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSLIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVSTCHQRGAHAIGGMAAFIPSKDPSVNEIAAEKVRADKEREAGDGFDGSWVAHPGLVPICREVFDGV 383          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|490697526|ref|WP_004561110.1| (malate synthase A [Amycolatopsis vancoresmycina] >gi|486079171|gb|EOD62821.1| malate synthase [Amycolatopsis vancoresmycina DSM 44592])

HSP 1 Score: 196.823 bits (499), Expect = 7.637e-51
Identity = 140/444 (31.53%), Postives = 194/444 (43.69%), Query Frame = 0

Query:   56 LVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            L + + TF+     L+  RR R+EE+       +P + F  ET+ +RD +        P + DR  E T P+    M ++ LNS A     D ED+ +P   NV                         + G+L+              F A++                  DF  E     T+ D            PATI  R RG H++E H  +DG+PVSA L+DF ++       L A    P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+    +   RRG  ++GGM   IP+ DPA+++ A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:   31 LAKLDNTFAGRRRELLDARRTRREELQSG---DKP-LGFLPETRRVRDDESWHVAPTAPGLEDRRVEITGPT-DRKMTVNALNSGAKVWLADFEDATSPTWHNV-------------------------IDGQLN-------------LFDAIRR---------------NIDFTTEAGKRYTIGD-----------DPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLYFFHNARQLLARGVGPYFYLPKLENHLEARLWNDVFLLAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSLIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVRTCHRRGAHAIGGMAAFIPSKDPAVNEIAVEKVRADKEREAGDGFDGSWVAHPGLVPICREVFDGV 383          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|695841064|ref|WP_032754365.1| (malate synthase A [Streptomyces alboviridis])

HSP 1 Score: 196.052 bits (497), Expect = 1.008e-50
Identity = 161/544 (29.60%), Postives = 233/544 (42.83%), Query Frame = 0

Query:   31 MEIVGGLTEEQQSLLTLEILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIR-DGDWKSETHRP-PNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV-----RALHR---GLAVLAKQVGEAKVRGTIKGPADPASIRQALADLVASISVENGGRESETLAAFLKALETS 564
            ++++G   +  + +LT E L  +    R +  F+     L+  RR R       +L     + F   T++IR D DW+    RP P + DR  E T P     MA++ L S A     D ED+ +P   N++ G  T+ +A+   I                      F+S A   +             R+TD                              RPATI VR RG H+ E H ++D +PV A L+DF ++   C        + P  YLPK+E+R+E   W  VF      L IP   +R+T +IETITA+ E EE+ Y LREHS G N GR+DYLF+ IK          N +  P+             ++LPD +   M   FL AY+    +   +RG  ++GGM   +P GD    + A AK+R+DK  EA  G  G W  HP   P  R  FD V       L R    + V A+++    VR T   P  PA +R  +A  +       GGR +  L   ++   T+
Sbjct:   10 VQVLGVPGDRHEEVLTPEALDFI---ARLDAAFAARRFDLLTERRRRS-----ALLRGGTPLDFSRATKSIREDPDWR--VARPAPGLTDRRVEITGPP-DRRMAVNALGSGAQVWMADFEDATSPTWENIVQGQLTLIDAIDRRID---------------------FTSEAGKEY-------------RLTD------------------------------RPATIMVRPRGWHLTEKHLVIDERPVPAALVDFGLYFFHCARRQLDAGSGPYFYLPKLENRYEARLWNDVFLLAQELLDIPRGTVRATVLIETITAAFEMEEILYELREHSAGLNAGRWDYLFSLIK----------NFAHRPD-------------FVLPDRSTVTMTAPFLRAYTELLVRTCHKRGAHAIGGMAAQVPGGDDGAQEAALAKVRLDKEREAEDGFDGSWVAHPGLVPVCRTVFDGVLGDRPHQLERTRDDVHVTAEEL--VAVRRTAGRPT-PAGVRDNIAVTLRYYDAWLGGRGAVALNGLMEDAATA 452          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|502988561|ref|WP_013223537.1| (malate synthase A [Amycolatopsis mediterranei] >gi|300783562|ref|YP_003763853.1| malate synthase [Amycolatopsis mediterranei U32] >gi|299793076|gb|ADJ43451.1| malate synthase [Amycolatopsis mediterranei U32] >gi|340524950|gb|AEK40155.1| malate synthase [Amycolatopsis mediterranei S699] >gi|398316217|gb|AFO75164.1| malate synthase [Amycolatopsis mediterranei S699] >gi|532227314|gb|AGT82293.1| malate synthase [Amycolatopsis mediterranei RB] >gi|641477024|gb|KDO11643.1| malate synthase [Amycolatopsis mediterranei] >gi|650433099|gb|KDU91126.1| malate synthase [Amycolatopsis mediterranei])

HSP 1 Score: 194.126 bits (492), Expect = 5.173e-50
Identity = 139/444 (31.31%), Postives = 191/444 (43.02%), Query Frame = 0

Query:   56 LVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            L + + TF+     L+  RR R+EE+      ++P + F  ET++IRD +        P + DR  E T P+    M ++ LNS A     D ED+ +                         P    V+ G+L+  D                             +    DF  +     T+ D            PATI  R RG H++E H  +DG+PVSA L+DF +FV      L A    P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+    +   RR   ++GGM   IP+ DP I+  A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:   31 LAKLDNTFAGRRRELLDARRVRREELQSG---EKP-LGFLPETRSIRDDESWHVAPTAPGLEDRRVEITGPT-DRKMTVNALNSGAKVWLADFEDATS-------------------------PTWHNVIDGQLNLYDA----------------------------IRRNIDFTTDAGKRYTIGD-----------EPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLFVFHNARQLVARGVGPYFYLPKLENHLEARLWNDVFLLAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSMIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVRTCHRRRAHAIGGMAAFIPSKDPEINATAIEKVRADKEREAGDGFDGSWVAHPGLVPVCREVFDGV 383          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|1089602706|emb|SEC04488.1| (malate synthase [Amycolatopsis tolypomycina])

HSP 1 Score: 193.356 bits (490), Expect = 1.218e-49
Identity = 137/444 (30.86%), Postives = 192/444 (43.24%), Query Frame = 0

Query:   56 LVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            L + + TF+     L+  RR R+EE+      ++P + F  ET+ IRD +        P + DR  E T P+    M ++ LNS A     D ED+ +                         P    V+ G+++  D                             +    DF  E     T+ D            PATI  R RG H++E H  +DG+PVSA L+DF ++       L A    P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+    +   RRG  ++GGM   IP+ DP++++ A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:   31 LAKLDNTFAGRRRELLDARRVRREELQSG---EKP-LGFLPETRRIRDDESWHVAPTAPGLEDRRVEITGPT-DRKMTVNALNSGAKVWLADFEDATS-------------------------PTWHNVIDGQINLYDA----------------------------IRRNIDFTTEAGKRYTIGD-----------DPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLYFFHNARQLLARGVGPYFYLPKLENHLEARLWNDVFLLAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSLIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVRTCHRRGAHAIGGMAAFIPSKDPSVNEIAVEKVRADKEREAGDGFDGSWVAHPGLVPVCREVFDGV 383          

BLAST of EWM30541.1 vs. NCBI_GenBank
Match: gi|1119522392|ref|WP_072481229.1| (malate synthase A [Amycolatopsis australiensis] >gi|1102126908|emb|SFW91781.1| malate synthase [Amycolatopsis australiensis])

HSP 1 Score: 192.971 bits (489), Expect = 1.498e-49
Identity = 141/469 (30.06%), Postives = 201/469 (42.86%), Query Frame = 0

Query:   31 MEIVGGLTEEQQSLLTLEILTHVRDLVRTERTFSPNLEGLMALRRARQEEIYKNILEKRPAMSFPAETQTIRDGDWKSETHRPPNMADRACENTHPSRAANMAIHGLNSKAASDFMDNEDSQAPLKANVLAGYRTIYEAVCGTIKDVRPNSKTVLKGRLSPQDLEPFSSNADATFQALQEAGVLNGHGRITDLLCEPDFVLEENVTSTVTDLKGLDGFLRERRPATIQVRVRGLHMLEGHFLVDGKPVSAPLLDFAVFVATCHAPLAAMAAQPTLYLPKMESRHETAWWAKVFAAMAAQLGIPPKAIRSTTMIETITASMEKEEMNYTLREHSLGQNKGRYDYLFNFIKRLTLPKSLQANLSPTPNNLELVGTHAPHRRYLLPDNAHTGMGVSFLEAYSNHTSQVNLRRGFDSVGGMVTNIPTGDPAIDKKAQAKLRVDKTNEAWKGDAGGWSGHPAFSPYVREPFDIV 499
            +E+ GG+      +LT   L     L + + TF+     L+  RR R+E++      ++P + F  ET+ IR+ +        P + DR  E T P+    M ++ LNS A     D ED+ +                         P    V+ G+++  D                             +    DF  E     T+ D            PATI  R RG H++E H  +DG+PVSA L+DF ++       L A  A P  YLPK+E+  E   W  VF     +LGIP   IR+T +IETITA+ E +E+ Y LREH+ G N GR+DY+F+ IK                         A    ++LPD A   M V F+ AY+    +   +RG  ++GGM   IP+ DP ++  A  K+R DK  EA  G  G W  HP   P  RE FD V
Sbjct:    9 IEVTGGVEGRFAEILTPAALDF---LAKLDNTFAGRRRELLDARRVRREDLQSG---EKP-LGFLPETRRIREDESWHVAPAAPGLEDRRVEITGPT-DRKMTVNALNSGAKVWLADFEDATS-------------------------PTWHNVIDGQINLYDA----------------------------IRRNIDFTTEAGKRYTIGD-----------DPATIVARPRGWHLVEKHIRIDGRPVSASLVDFGLYFFHNARQLIARGAGPYFYLPKLENHLEARLWNDVFLMAQRELGIPRGTIRATVLIETITAAFEMDEILYELREHAAGLNAGRWDYIFSLIKNFA----------------------AHGADFVLPDRAQVTMTVPFMRAYTELLVRTCHKRGAHAIGGMAAFIPSKDPEVNATAIEKVRADKEREAGDGFDGSWVAHPGLVPVCREVFDRV 383