EWM29436.1, cds1237 (CDS) Nannochloropsis gaditana

Overview
NameEWM29436.1
Unique Namecds1237
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationCM002457.1:120808..121185 -
Alignment locationCM002457.1:120735..120758 -

Link to JBrowse

Properties
Property NameValue
Protein idEWM29436.1
Producthistidyl-trna synthetase
Orig transcript idgnl|cribi|Naga_103156g1.2573.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
CM002457.1supercontigCM002457.1:120808..121185 -
CM002457.1supercontigCM002457.1:120735..120758 -
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Biological Process
TermDefinition
GO:0006412translation
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: INTERPRO
TermDefinition
IPR004516HisRS/HisZ
IPR004154Anticodon-bd
Homology
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|585111970|gb|EWM29436.1| (histidyl-trna synthetase [Nannochloropsis gaditana])

HSP 1 Score: 266.159 bits (679), Expect = 3.692e-90
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRRRWRRS 133
            MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRRRWRRS
Sbjct:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRRRWRRS 133          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|1032153626|gb|OAO16942.1| (histidyl-tRNA synthetase [Blastocystis sp. ATCC 50177/Nand II] >gi|1032154655|gb|OAO17877.1| histidyl-tRNA synthetase [Blastocystis sp. ATCC 50177/Nand II])

HSP 1 Score: 105.531 bits (262), Expect = 8.446e-24
Identity = 59/129 (45.74%), Postives = 80/129 (62.02%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRRR 129
            MF+  G   PCVG+SIGVERV TI+E ++Q+             +GGL    + +P   VLVA++G +M  HRMR+A  LW  GI AE+  QENPKL+KQ+ YA E GI ++V+VG +E K G V  + 
Sbjct:  748 MFNAGGKDIPCVGISIGVERVFTILEKQAQE-------------KGGL----KCTP-CSVLVASVGKDMTVHRMRVAADLWRNGIAAEFGYQENPKLQKQLAYALEAGINWVVVVGEEEIKEGKVNLKN 858          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|397596695|gb|EJK56825.1| (hypothetical protein THAOC_23204, partial [Thalassiosira oceanica])

HSP 1 Score: 104.76 bits (260), Expect = 1.670e-23
Identity = 59/126 (46.83%), Postives = 78/126 (61.90%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVK 126
            MF  +G  TPCVGVS+G+ERV T+ME R +     +E GG             + P V+VLVA+ G N+L  RMRLAR+LW A I AE+  QENPKLK ++  A ++ IPF+VI G +E + G  K
Sbjct:  680 MFQEAGKVTPCVGVSVGIERVFTLMEERLR-----KEQGGS-----------IKRPNVKVLVASAGENLLHERMRLARVLWDANISAEFSQQENPKLKFEIANALDRQIPFMVISGEEEAREGKCK 789          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|950900885|ref|XP_014530234.1| (histidine-tRNA ligase [Blastocystis sp. subtype 4] >gi|902864846|gb|KNB46792.1| histidine-tRNA ligase [Blastocystis sp. subtype 4])

HSP 1 Score: 104.375 bits (259), Expect = 1.844e-23
Identity = 57/125 (45.60%), Postives = 74/125 (59.20%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTV 125
            MF+  G   PCVG+SIGVERV TI+E ++Q A           GE    P         VLV +IG +M   RM++A  LW AGI AE+  QENPKL+KQ+ YA E GI ++V++G +E K G V
Sbjct: 1082 MFNAGGKDIPCVGISIGVERVFTILEKKAQDA-----------GELRCKP-------CSVLVTSIGKDMTAMRMKVAATLWHAGISAEFGYQENPKLQKQLAYALETGINWVVVMGEEEMKEGKV 1188          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|224010758|ref|XP_002294336.1| (histidyl tRNA synthetase [Thalassiosira pseudonana CCMP1335] >gi|220969831|gb|EED88170.1| histidyl tRNA synthetase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 102.834 bits (255), Expect = 3.705e-23
Identity = 56/131 (42.75%), Postives = 77/131 (58.78%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRRRWR 131
            MF  +G  TPCVGVS+G+ERV T+ME R +Q         E GG         + P V VLVA+ G N++  RM+L  +LW A I AE+  Q+NPKLK ++  A ++ IPF+VI G +E K G  K +  +
Sbjct:  312 MFQEAGKVTPCVGVSVGIERVFTLMEERLRQ---------EQGGS-------IKQPNVSVLVASAGENLMQERMKLTNILWDANISAEFSQQDNPKLKFEIANALDRDIPFMVISGEEEAKEGRCKVKDLK 426          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|855305717|ref|XP_012895514.1| (uncharacterized protein [Blastocystis hominis] >gi|300121084|emb|CBK21466.2| unnamed protein product [Blastocystis hominis])

HSP 1 Score: 101.679 bits (252), Expect = 2.018e-22
Identity = 57/131 (43.51%), Postives = 78/131 (59.54%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRRRWR 131
            MFS SG Q PCVG+SIGVERV TI+E ++               EG L    R +P  +V VA+ G  +   RMR+A +LW  GI AE+  Q NPKL+KQ+ YA E G+ ++V+VG +E K+G V  +  +
Sbjct:  754 MFSASGKQIPCVGISIGVERVFTILEKKA-------------ADEGTL----RCAP-CDVFVASAGKELAAQRMRIAGMLWRNGIKAEFGYQANPKLQKQLTYALESGVKWIVVVGEEEMKQGKVNLKNLQ 866          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|260780861|ref|XP_002585554.1| (hypothetical protein BRAFLDRAFT_274629 [Branchiostoma floridae] >gi|229270558|gb|EEN41565.1| hypothetical protein BRAFLDRAFT_274629 [Branchiostoma floridae])

HSP 1 Score: 100.908 bits (250), Expect = 2.506e-22
Identity = 55/128 (42.97%), Postives = 71/128 (55.47%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRR 128
            MF   G + PCVGVSIG+ER+ +IMEA+++ A                   + R+   +V VA+   NML  RM+L  LLW  GI  E   ++NPKL  Q +YA E GIPFL I+G  E K G VK R
Sbjct:  379 MFDPKGKKVPCVGVSIGIERLFSIMEAKAEAAEG-----------------KVRTTETQVYVASGQKNMLEERMKLCNLLWEGGIKTELSYKKNPKLLNQFQYAEEHGIPFLAIIGEQELKDGVVKVR 489          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|584018066|ref|XP_006803758.1| (PREDICTED: histidine--tRNA ligase, cytoplasmic-like [Neolamprologus brichardi])

HSP 1 Score: 99.7525 bits (247), Expect = 3.198e-22
Identity = 53/128 (41.41%), Postives = 75/128 (58.59%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRR 128
            MF   G + PCVGVSIG+ER+ +IME   Q+A A+              P + R+  V+++VA+   N+L  R++L   LW AGI AE + ++NPKL  Q++Y  E GIP + I+G  E K G VK R
Sbjct:  245 MFDPKGRRVPCVGVSIGIERIFSIME---QKAEAS--------------PEKVRTTEVQIMVASAQKNLLEERLQLVSELWNAGIKAEVMYKKNPKLLSQLQYCEEAGIPLVAILGEQELKDGVVKLR 355          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|260786598|ref|XP_002588344.1| (hypothetical protein BRAFLDRAFT_114648 [Branchiostoma floridae] >gi|229273505|gb|EEN44355.1| hypothetical protein BRAFLDRAFT_114648 [Branchiostoma floridae])

HSP 1 Score: 100.523 bits (249), Expect = 3.388e-22
Identity = 55/128 (42.97%), Postives = 71/128 (55.47%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRR 128
            MF   G + PCVGVSIG+ER+ +IMEA+++ A                   + R+   +V VA+   NML  RM+L  LLW  GI  E   ++NPKL  Q +YA E GIPFL I+G  E K G VK R
Sbjct:  336 MFDPKGKKVPCVGVSIGIERLFSIMEAKAEAAEG-----------------KVRTTETQVYVASGQKNMLEERMKLCNLLWEGGIKTELSYKKNPKLLNQFQYAEEHGIPFLAIIGEQELKDGVVKVR 446          
BLAST of EWM29436.1 vs. NCBI_GenBank
Match: gi|736216923|ref|XP_010778804.1| (PREDICTED: histidine--tRNA ligase, cytoplasmic-like [Notothenia coriiceps])

HSP 1 Score: 97.0561 bits (240), Expect = 3.516e-22
Identity = 52/128 (40.62%), Postives = 74/128 (57.81%), Query Frame = 0
Query:    1 MFSVSGTQTPCVGVSIGVERVLTIMEARSQQARAAREGGGEGGGEGGLPPRRRRSPFVEVLVATIGANMLPHRMRLARLLWAAGIPAEYLPQENPKLKKQMEYAHEQGIPFLVIVGGDERKRGTVKRR 128
            MF   G + PCVGVSIG+ER+ +IME   Q+A A+               +R R+  V+V+V +   N+L  R++L   LW AGI AE + ++NPKL  Q++Y  + GIP + I+G  E K G VK R
Sbjct:  110 MFDPKGRKVPCVGVSIGIERIFSIME---QKAEAS--------------AQRVRTTEVQVMVVSAQKNLLEERLKLLTELWNAGIKAEVIYKKNPKLLSQLQYCEDSGIPLVAILGEQELKDGVVKLR 220          
The following BLAST results are available for this feature:
BLAST of EWM29436.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585111970|gb|EWM29436.1|3.692e-90100.00histidyl-trna synthetase [Nannochloropsis gaditana... [more]
gi|1032153626|gb|OAO16942.1|8.446e-2445.74histidyl-tRNA synthetase [Blastocystis sp. ATCC 50... [more]
gi|397596695|gb|EJK56825.1|1.670e-2346.83hypothetical protein THAOC_23204, partial [Thalass... [more]
gi|950900885|ref|XP_014530234.1|1.844e-2345.60histidine-tRNA ligase [Blastocystis sp. subtype 4]... [more]
gi|224010758|ref|XP_002294336.1|3.705e-2342.75histidyl tRNA synthetase [Thalassiosira pseudonana... [more]
gi|855305717|ref|XP_012895514.1|2.018e-2243.51uncharacterized protein [Blastocystis hominis] >gi... [more]
gi|260780861|ref|XP_002585554.1|2.506e-2242.97hypothetical protein BRAFLDRAFT_274629 [Branchiost... [more]
gi|584018066|ref|XP_006803758.1|3.198e-2241.41PREDICTED: histidine--tRNA ligase, cytoplasmic-lik... [more]
gi|260786598|ref|XP_002588344.1|3.388e-2242.97hypothetical protein BRAFLDRAFT_114648 [Branchiost... [more]
gi|736216923|ref|XP_010778804.1|3.516e-2240.63PREDICTED: histidine--tRNA ligase, cytoplasmic-lik... [more]
back to top
Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna1238rna1238Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications