EWM20533.1, cds10140 (CDS) Nannochloropsis gaditana

Overview
NameEWM20533.1
Unique Namecds10140
TypeCDS
OrganismNannochloropsis gaditana (N. gaditana B-31)
Alignment locationKK037264.1:43761..43788 +
Alignment locationKK037264.1:43941..44008 +
Alignment locationKK037264.1:44222..44308 +
Alignment locationKK037264.1:44808..44952 +
Alignment locationKK037264.1:45098..45213 +

Link to JBrowse

Properties
Property NameValue
Protein idEWM20533.1
ProductPeptidase C2, calpain, catalytic domain protein
Orig transcript idgnl|cribi|Naga_101712g1.8966.mrna
GbkeyCDS
Mutants
Expression
No biomaterial libraries express this feature.
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
KK037264.1supercontigKK037264.1:43761..43788 +
KK037264.1supercontigKK037264.1:43941..44008 +
KK037264.1supercontigKK037264.1:44222..44308 +
KK037264.1supercontigKK037264.1:44808..44952 +
KK037264.1supercontigKK037264.1:45098..45213 +
Analyses
This CDS is derived from or has results from the following analyses
Analysis NameDate Performed
GO annotation for N. gaditana B312020-04-08
BLAST analysis for N. gaditana B-312020-04-07
InterPro analysis for N. gaditana B-312020-04-06
Gene prediction for N. gaditana B-312014-02-18
Annotated Terms
The following terms have been associated with this CDS:
Vocabulary: Molecular Function
TermDefinition
GO:0016787hydrolase activity
GO:0004198calcium-dependent cysteine-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0019538protein metabolic process
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0005622intracellular
Vocabulary: INTERPRO
TermDefinition
IPR001300Peptidase_C2_calpain_cat
Homology
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|585100218|gb|EWM20533.1| (Peptidase C2, calpain, catalytic domain protein [Nannochloropsis gaditana])

HSP 1 Score: 305.449 bits (781), Expect = 3.119e-105
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTGR 147
            MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTGR
Sbjct:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTGR 147          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|1183427323|gb|ORY51621.1| (cysteine proteinase [Rhizoclosmatium globosum])

HSP 1 Score: 97.0561 bits (240), Expect = 1.249e-20
Identity = 62/150 (41.33%), Postives = 79/150 (52.67%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKE--LKD-VAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTGR 147
            + DLT FP E I F   GFD E FW RL SFQ  GF MGA CP S +GLVG HAYS+L V E  ++D + +G+ + + +YF              G +R  + DD       GV             T+ G LRL++LRNPWG  EW G+
Sbjct:  357 LFDLTGFPTESIPFGGIGFDSELFWTRLLSFQSQGFLMGAVCPQSGDGLVGGHAYSLLQVVEESIEDGLVIGKTKNLHDYF------------DAGVKR--KWDD---TQEFGV-------------TDHGTLRLIKLRNPWGKVEWKGK 476          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|145349968|ref|XP_001419397.1| (predicted protein [Ostreococcus lucimarinus CCE9901] >gi|144579628|gb|ABO97690.1| predicted protein [Ostreococcus lucimarinus CCE9901])

HSP 1 Score: 89.7373 bits (221), Expect = 4.777e-18
Identity = 53/146 (36.30%), Postives = 68/146 (46.58%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            +HDLT    EMI F +  FD E FW RL SF  AGF +G +  +S EG+VG HAYS+L V+E+  V  G Q K+ E                                       L    P   TE  +LRL+++RNPWG +EW G
Sbjct:  358 LHDLTGSATEMISFSSTYFDSEEFWVRLVSFSSAGFPLGCATSFSAEGIVGHHAYSILEVREMHGVKKGVQTKLPEV--------------------------------------LHGKKPAVETE--MLRLLKIRNPWGKKEWRG 463          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|1199291959|gb|OUS47049.1| (hypothetical protein BE221DRAFT_72852 [Ostreococcus tauri])

HSP 1 Score: 88.1965 bits (217), Expect = 1.589e-17
Identity = 55/146 (37.67%), Postives = 69/146 (47.26%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            +HDLT    E I F  + FD E  WARL SF  AGF +G +  +S EG+VG HAYSVL V+E+  V  G Q K+ E +A          RG+ A                               E   LRL+++RNPWG REW G
Sbjct:  366 LHDLTGSATEQIDFHANDFDSEECWARLMSFSAAGFPLGCATSFSAEGIVGHHAYSVLEVREVVGVKKGVQTKLTEVYA----------RGKDA----------------------------LDVEVENLRLLKIRNPWGKREWRG 473          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|308807110|ref|XP_003080866.1| (CG1391-PB, isoform B (ISS) [Ostreococcus tauri] >gi|116059327|emb|CAL55034.1| Peptidase C2, calpain family [Ostreococcus tauri])

HSP 1 Score: 88.1965 bits (217), Expect = 1.717e-17
Identity = 55/146 (37.67%), Postives = 69/146 (47.26%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            +HDLT    E I F  + FD E  WARL SF  AGF +G +  +S EG+VG HAYSVL V+E+  V  G Q K+ E +A          RG+ A                               E   LRL+++RNPWG REW G
Sbjct:  366 LHDLTGSATEQIDFHANDFDSEECWARLMSFSAAGFPLGCATSFSAEGIVGHHAYSVLEVREVVGVKKGVQTKLTEVYA----------RGKDA----------------------------LDVEVENLRLLKIRNPWGKREWRG 473          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|219116891|ref|XP_002179240.1| (predicted protein [Phaeodactylum tricornutum CCAP 1055/1] >gi|217409131|gb|EEC49063.1| predicted protein [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 86.2705 bits (212), Expect = 7.934e-17
Identity = 55/146 (37.67%), Postives = 72/146 (49.32%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            + DLT  P E I F +D  D +  WARL SF +AGF +G +     EGLVG HAYS+L V E+ +  +G+Q+KV +YF+  S             R   G             P+     P        +RLVR+RNPWG REW G
Sbjct:  464 LTDLTGAPTETIIF-SDLIDLDELWARLLSFHQAGFLVGVATSRGGEGLVGGHAYSLLDVIEINNSLIGEQKKVTDYFSSPS----------KKHRKLTG-------------PNYDTCAP--------IRLVRIRNPWGKREWKG 577          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|255075503|ref|XP_002501426.1| (predicted protein [Micromonas commoda] >gi|226516690|gb|ACO62684.1| predicted protein [Micromonas commoda])

HSP 1 Score: 82.0333 bits (201), Expect = 2.368e-15
Identity = 52/150 (34.67%), Postives = 70/150 (46.67%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASC----PYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            + DLT  P E I+ D  GF  E  W +L S+  A F MG +      + + GLVG HAYSV+ V+EL   +VG+Q K+  + A   V  V +  G G                       PP           LRL+R+RNPWG +EW+G
Sbjct:  410 LLDLTGCPTETIRLDASGFKSEEAWRKLLSYAAAKFPMGCATQGDPSHREVGLVGNHAYSVMEVRELHGASVGRQTKLTSFVAKERVEDVDAAAGGGG----------------------PP-----------LRLLRIRNPWGRKEWSG 526          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|1210527406|dbj|GAX14041.1| (calpain-15 [Fistulifera solaris])

HSP 1 Score: 76.2554 bits (186), Expect = 2.349e-13
Identity = 48/146 (32.88%), Postives = 65/146 (44.52%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            + DLT  P E        F  +  W  L ++Q  GF MG +     +GL+G HAYS+L V +L D+ VG Q KV +YF                         G+N +  V +P           +   +RLVR+RNPWG REW G
Sbjct:  451 LQDLTGAPTETHLL----FAADHLWPILMTYQREGFLMGVATARGGDGLIGGHAYSILDVIQLDDMIVGDQPKVTDYF-------------------------GQNSQKSVKVPE----------KRQTIRLVRIRNPWGKREWKG 557          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|1210523710|dbj|GAX17392.1| (calpain-15 [Fistulifera solaris])

HSP 1 Score: 75.8702 bits (185), Expect = 3.172e-13
Identity = 49/146 (33.56%), Postives = 62/146 (42.47%), Query Frame = 0
Query:    1 MHDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            + DLT  P E        F  E  W  L ++Q  GF MG +     +GLVG HAYS+L V +L D+ VG Q KV +YF+     SV                                     L +   +RLVR+RNPWG REW G
Sbjct:  456 LQDLTGAPTETHLL----FAAEHLWPILMTYQREGFLMGVATARGGDGLVGGHAYSILDVIQLDDMIVGDQSKVTDYFSQNPQKSV-----------------------------------KVLEQRQTIRLVRIRNPWGKREWKG 562          
BLAST of EWM20533.1 vs. NCBI_GenBank
Match: gi|303279130|ref|XP_003058858.1| (predicted protein [Micromonas pusilla CCMP1545] >gi|226460018|gb|EEH57313.1| predicted protein [Micromonas pusilla CCMP1545])

HSP 1 Score: 71.633 bits (174), Expect = 9.543e-12
Identity = 54/145 (37.24%), Postives = 69/145 (47.59%), Query Frame = 0
Query:    2 HDLTSFPCEMIQFDTDGFDREAFWARLCSFQEAGFTMGASCPYSQEGLVGCHAYSVLCVKELKDVAVGQQRKVLEYFAPRSVASVVSTRGRGAQRAGEGDDGGKNDRAGVHLPSLPPSPPPFLTEDGVLRLVRLRNPWGCREWTG 146
            H   + P + +      FD    W ++ S+ EAGF MG +      GLVG HAYSVL V++L  VAVG+Q K L+ F  R V  V          A        +  A     +   SPPP       LRLVR+RNPWG +EW G
Sbjct:  444 HSSPALPFQRLTGGGGAFDEARAWEKVLSYCEAGFPMGCATSSGGRGLVGGHAYSVLDVRQLHGVAVGRQTK-LDGFVEREVEIV--------DPAPALVSASASKYASSAAAADASSPPP-------LRLVRVRNPWGRKEWNG 572          
The following BLAST results are available for this feature:
BLAST of EWM20533.1 vs. NCBI_GenBank
Analysis Date: 2020-04-07 (BLAST analysis for N. gaditana B-31)
Total hits: 10
Match NameE-valueIdentityDescription
gi|585100218|gb|EWM20533.1|3.119e-105100.00Peptidase C2, calpain, catalytic domain protein [N... [more]
gi|1183427323|gb|ORY51621.1|1.249e-2041.33cysteine proteinase [Rhizoclosmatium globosum][more]
gi|145349968|ref|XP_001419397.1|4.777e-1836.30predicted protein [Ostreococcus lucimarinus CCE990... [more]
gi|1199291959|gb|OUS47049.1|1.589e-1737.67hypothetical protein BE221DRAFT_72852 [Ostreococcu... [more]
gi|308807110|ref|XP_003080866.1|1.717e-1737.67CG1391-PB, isoform B (ISS) [Ostreococcus tauri] >g... [more]
gi|219116891|ref|XP_002179240.1|7.934e-1737.67predicted protein [Phaeodactylum tricornutum CCAP ... [more]
gi|255075503|ref|XP_002501426.1|2.368e-1534.67predicted protein [Micromonas commoda] >gi|2265166... [more]
gi|1210527406|dbj|GAX14041.1|2.349e-1332.88calpain-15 [Fistulifera solaris][more]
gi|1210523710|dbj|GAX17392.1|3.172e-1333.56calpain-15 [Fistulifera solaris][more]
gi|303279130|ref|XP_003058858.1|9.543e-1237.24predicted protein [Micromonas pusilla CCMP1545] >g... [more]
back to top
Relationships

This CDS is a part of the following mRNA feature(s):

Feature NameUnique NameSpeciesType
rna10171rna10171Nannochloropsis gaditana (N. gaditana B-31)mRNA


Sequences
Synonyms
Publications