EWM20588.1, cds10087 (CDS) Nannochloropsis gaditana

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|585100327|gb|EWM20588.1| (long-chain acyl- synthetase 7 [Nannochloropsis gaditana])

HSP 1 Score: 1425.61 bits (3689), Expect = 0.000e+0
Identity = 689/689 (100.00%), Postives = 689/689 (100.00%), Query Frame = 0

Query:    1 MVYVPHRKKFYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAAAPECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYATRDLHQGSKARL 689
            MVYVPHRKKFYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAAAPECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYATRDLHQGSKARL
Sbjct:    1 MVYVPHRKKFYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAAAPECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYATRDLHQGSKARL 689          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|224008917|ref|XP_002293417.1| (Long-chain-fatty-acid--CoA ligase [Thalassiosira pseudonana CCMP1335] >gi|220970817|gb|EED89153.1| Long-chain-fatty-acid--CoA ligase [Thalassiosira pseudonana CCMP1335])

HSP 1 Score: 638.262 bits (1645), Expect = 0.000e+0
Identity = 339/680 (49.85%), Postives = 442/680 (65.00%), Query Frame = 0

Query:   11 YSIEVPNTETAT-AGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSE-GHAAPYVFESYEEAARRIDNLASALEHEG---LCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAA--APECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRY----PQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDW-TTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLP--VDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELY 676
            +SIEVP+T   T  GPI R PD    P L   G  T+YE  RR   + P  P LG R   S  G+A PYV+ SY E   RID +A  LE  G   L     DG+ LLG+YMKNC EWI+A+QA + + GATVP YDTLGPD V ++++ T ++ VVC  +ELE L  A  +  CP   + +L   V +     +A   GLK+ S   +E LG +        H PPDP D+ATF YTSGTTG PKGAL+TH N ++    G   E+   P D H SYLPLPH+FERV++  +L +GA++ F++G P  L+ED+ A RPT +P VPRVLN+++DK++AG+ A   + + LF+ A  AK   L E G L   +WDR++F K++RA+GLD +R+MV+GSAP++P V+ F R L   P+ EGYGQTE  A  +++ P D  + GHVGG     E+ L DVPEMGYL TD  H              +PC GRGEIC+RGP++FKGYYK  +KT ETID +GWLHSGDVG+W P+G+L IIDRKKNIFKLAQGEYVAAEKIENVL+QS +I Q FV+GDS ++ LVA++VPD + V+ WAR ++LP     SF +LC    LK++IL EI   SK  GLHGFE  KA++LE   F+  +GL+TPT KLKR Q RD Y+  I+ +Y
Sbjct:    5 WSIEVPSTSRPTHRGPIHRSPDPLATPTLGAHGVRTLYEAIRRGRDVNPLGPCLGFRATSSNSGYATPYVYGSYGECVARIDAVACGLERGGGERLLDRNEDGMLLLGIYMKNCMEWILAEQAVYCIGGATVPFYDTLGPDTVRFILEHTGMSCVVCSRKELERLCEAKKSGTCPKFHSVILVDGVTA-QATDMANGAGLKVVSFAKIEALGAQIVPTEGHKHTPPDPKDIATFCYTSGTTGDPKGALITHENIMSA--VGGLNEIPILPTDRHISYLPLPHIFERVVVAQMLTSGASIAFFRGDPLLLIEDIVACRPTMVPMVPRVLNKIHDKIVAGMAAKGGVTEKLFKMAMEAKTLGLQE-GRLKHPLWDRILFNKIKRALGLDCVRLMVSGSAPLSPKVMIFFRCLLGVPVMEGYGQTEGAAAATISHPDDIASVGHVGGPTDCVEIVLEDVPEMGYLSTDTSHN------------GEPCRGRGEICIRGPNVFKGYYKDEEKTKETIDEEGWLHSGDVGLWMPDGQLKIIDRKKNIFKLAQGEYVAAEKIENVLLQSLLIGQIFVHGDSFQTYLVAVIVPDEDPVRAWAR-DNLPDLATASFLDLCNHDKLKQEILSEIRRLSKLNGLHGFETVKAVHLEPNIFSAESGLVTPTFKLKRPQLRDHYAKEIEAMY 667          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|57902451|gb|AAW58006.1| (long chain acyl-coA synthetase [Thalassiosira pseudonana])

HSP 1 Score: 636.336 bits (1640), Expect = 0.000e+0
Identity = 338/680 (49.71%), Postives = 441/680 (64.85%), Query Frame = 0

Query:   11 YSIEVPNTETAT-AGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSE-GHAAPYVFESYEEAARRIDNLASALEHEG---LCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAAAPE--CPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRY----PQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDW-TTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLP--VDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELY 676
            +SIEVP+T   T  GPI R PD    P L   G  T+YE  RR   + P  P LG R   S  G+A PYV+ SY E   RID +A  LE  G   L     DG+ LLG+YMKNC EWI+A+QA + + GATVP YDTLGPD V ++++ T ++ VVC  +ELE L  A     CP   + +L   V +     +A   GLK+ S   +E LG +        H PPDP D+ATF YTSGTTG PKGAL+TH + ++    G   E+   P D H SYLPLPH+FERV++  +L +GA++ F++G P  L+ED+ A RPT +P VPRVLN+++DK++AG+ A   + + LF+ A  AK   L E G L   +WDR++F K++RA+GLD +R+MV+GSAP++P V+ F R L   P+ EGYGQTE  A  +++ P D  + GHVGG     E+ L DVPEMGYL TD  H              +PC GRGEIC+RGP++FKGYYK  +KT ETID +GWLHSGDVG+W P+G+L IIDRKKNIFKLAQGEYVAAEKIENVL+QS +I Q FV+GDS ++ LVA++VPD + V+ WAR ++LP     SF +LC    LK++IL EI   SK  GLHGFE  KA++LE   F+  +GL+TPT KLKR Q RD Y+  I+ +Y
Sbjct:    5 WSIEVPSTSRPTHRGPIHRSPDPLATPTLGAHGVRTLYEAIRRGRDVNPLGPCLGFRATSSNSGYATPYVYGSYGECVARIDAVACGLERGGGERLLDRNEDGMLLLGIYMKNCMEWILAEQAVYCIGGATVPFYDTLGPDTVRFILEHTGMSCVVCSRKELERLCEAKKSGTCPKFHSVILVDGVTA-QATDMANGAGLKVVSFAKIEALGAQIVPTEGHKHTPPDPKDIATFCYTSGTTGDPKGALITHEDIMSA--VGGLNEIPILPTDRHISYLPLPHIFERVVVAQMLTSGASIAFFRGDPLLLIEDIVACRPTMIPMVPRVLNKIHDKIVAGMAAKGGVTEKLFKMAMDAKTLGLQE-GRLKHPLWDRILFNKIKRALGLDCVRLMVSGSAPLSPKVMIFFRCLLGVPVMEGYGQTEGAAAATISHPDDIASVGHVGGPTDCVEIVLEDVPEMGYLSTDTSHN------------GEPCRGRGEICIRGPNVFKGYYKDEEKTKETIDEEGWLHSGDVGLWMPDGQLKIIDRKKNIFKLAQGEYVAAEKIENVLLQSLLIGQIFVHGDSFQTYLVAVIVPDEDPVRAWAR-DNLPDLATASFLDLCNHDKLKQEILSEIRRLSKLNGLHGFETVKAVHLEPNIFSAESGLVTPTFKLKRPQLRDHYAKEIEAMY 667          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|1210526630|dbj|GAX14588.1| (long-chain acyl-CoA synthetase [Fistulifera solaris])

HSP 1 Score: 632.098 bits (1629), Expect = 0.000e+0
Identity = 334/680 (49.12%), Postives = 436/680 (64.12%), Query Frame = 0

Query:   10 FYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVA---AAPECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFR----YPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKAL-EVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTT-GHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQ---ESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYA 677
             +S  VP T T T G IRR  + +++P+L   G  T+YE FRR  A+ P  P +G R + + G A PY++ SY E   RI++ A+ LE   L  PT D L +L LY+KNC EWI+A+Q+ F L GATVPLYDTLGPD V +V+ QTE  +VVC   E+E L     A+ + P     ++    ++      A + GL++ S   +E +G          H PP P D+ATF YTSGTTG PKGALLTH N ++         EV    DD H SYLPL H+FER ++  V   GA++ F++G P  L+EDLQA RPT +PA PRVLN++YDK+  GV AA  +K  LF  A AAK   L ++G L   ++DRL+F+K+++ +G+D +RVM++GSAP++ +V+ F R +   P+ EGYGQTE  A  +++ P D +T GHVG      E+ L++VPEMGYLH+DR+H    RG        Q C GRGEIC+RGP IFKGYYK  DKT E ID  GWLHSGDVGMW  +G L IIDRKKNIFKLAQGEYVA EKIEN+L +S +IAQ FVYGDS +S+LVAIVVPD E V+    +   E L     FAE+C+ + +KE I+ +IE  SK  GL GFE  KAI+LE+  F+  N L+TPT KLKR + RDRY   I+ LYA
Sbjct:   23 LWSTVVPQTSTETRGNIRRSYNPQVHPELGIKGARTLYESFRRGAALNPLGPCMGFRAVSTTGFATPYIYTSYSECVARINSFAAGLETLNLVKPTSDNLMVLCLYVKNCMEWILAEQSVFALGGATVPLYDTLGPDTVRFVLHQTEAQSVVCTRAEVEALCRVKDASDKVPVHFQTIIVIDGVTPEVADQAGRAGLEIISYAKVEAVGAHRIATQGHRHRPPQPGDIATFCYTSGTTGDPKGALLTHENMVSSMAGISQFPEVAIRTDDRHLSYLPLAHIFERTVISQVFLAGASVAFFRGDPTLLIEDLQACRPTLLPAAPRVLNKIYDKITTGVAAAGGMKSKLFNAALAAKTEGL-KKGHLHHALYDRLIFSKIKKGLGMDYLRVMISGSAPLSENVMTFFRCMLGIPVVEGYGQTEGAASGTISHPDDISTVGHVGCPVGSVEIVLMNVPEMGYLHSDREH----RG--------QKCYGRGEICLRGPSIFKGYYKAEDKTKEAIDEDGWLHSGDVGMWRQDGSLQIIDRKKNIFKLAQGEYVAPEKIENLLGRSPLIAQCFVYGDSHQSQLVAIVVPDEEPVRHSVGRGVDEHLSASSPFAEICQSAKVKEAIMKDIEKLSKENGLQGFETVKAIHLEASLFSVENDLMTPTFKLKRQKVRDRYEKEIETLYA 689          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|1210519121|dbj|GAX22119.1| (long-chain acyl-CoA synthetase [Fistulifera solaris])

HSP 1 Score: 629.787 bits (1623), Expect = 0.000e+0
Identity = 334/680 (49.12%), Postives = 433/680 (63.68%), Query Frame = 0

Query:   10 FYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVA---AAPECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFR----YPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKAL-EVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTT-GHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQ---ESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYA 677
             +S  VP T T T G IRR  +  ++P+L   G  T+YE FRR  A+ P  P +G R + + G A PY++ SY E   RI++ A+ LE   L  PT D L +L LY+KNC EWI+A+Q  F L GATVPLYDTLGPD V +V+ QTE  +VVC   E+E L     A  + P     ++    ++      A + GL++ S   +E +G          H PP P D+ATF YTSGTTG PKGALLTH N ++         EV    DD H SYLPL H+FER ++  V   GA++ F++G P  L+EDLQA RPT +PA PRVLN++YDK+  GV AA  +K  LF  A AAK   L ++G L   ++DRL+F+K+++ +G+D +RVM++GSAP++ +V+ F R +   P+ EGYGQTE  A  +++ P D +T GHVG      E+ L++VPEMGYLH+DR+H    RG        Q C GRGEIC+RGP IFKGYYK  DKT E ID  GWLHSGDVGMW  +G L IIDRKKNIFKLAQGEYVA EKIEN+L +S +IAQ FVYGDS +S+LVAIVVPD E V+    +   E+L    SFAE+C+ + +KE I+ +I   SK  GL GFE  KAI+LE+  FT  N  +TPT KLKR + RDRY   I+ LYA
Sbjct:   17 LWSTVVPQTSTETRGNIRRSFNPLVHPELGIKGARTLYESFRRGAALNPLGPCMGFRAVSTTGFATPYIYTSYSECVARINSFAAGLETLNLVKPTSDNLMVLCLYVKNCMEWILAEQGVFALGGATVPLYDTLGPDTVRFVLHQTEAQSVVCTRAEVEALCRVKDAGDKVPVHFQTIIVIDGVTPEVADQAGRAGLEIISYAKVEAVGAHRIATQGHRHCPPQPGDIATFCYTSGTTGDPKGALLTHENMVSSMAGISQFPEVAIRADDRHLSYLPLAHIFERTVISQVFLAGASVAFFRGDPTLLIEDLQACRPTLLPAAPRVLNKIYDKITTGVAAAGGVKSKLFNAALAAKTEGL-KKGHLHHALYDRLIFSKIKKGLGMDHLRVMISGSAPLSENVMTFFRCMLGIPVVEGYGQTEGAASGTISHPDDISTVGHVGCPVGSVEIVLMNVPEMGYLHSDREH----RG--------QKCYGRGEICLRGPSIFKGYYKAEDKTREAIDEDGWLHSGDVGMWRQDGSLQIIDRKKNIFKLAQGEYVAPEKIENILGRSPLIAQCFVYGDSHQSQLVAIVVPDEEPVRHSVGRGLDENLSATSSFAEICQSTKVKEAIMKDIVKLSKENGLQGFETVKAIHLEASLFTVENDFMTPTFKLKRQKVRDRYEKEIETLYA 683          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|1072231975|gb|OEU16707.1| (acetyl-CoA synthetase-like protein [Fragilariopsis cylindrus CCMP1102])

HSP 1 Score: 613.994 bits (1582), Expect = 0.000e+0
Identity = 321/680 (47.21%), Postives = 426/680 (62.65%), Query Frame = 0

Query:   12 SIEVPNTETATA-GPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTP--DG-LRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENL--VAAAPECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRYPQP----HFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLT-VYCAGKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDW-TTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLP--VDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYA 677
            S  VP T      G IRR  D   +P+L + GC T+YE FRR   I P    LG R I + G A PY++ SY E   RI+  A+ L+   L PPT   DG + L+GLYMKNC EW IA+QA F + GATVP YDTLGP++V +++KQT    VV   +EL+ L  V  +  CP  +  VL   V + H   +A++ GL + S   +E +G          H PP   D+ TF YTSGTTG PKGALLTH N ++ +   G     E    D H SYLPL H+FER+++  +   GA++ F++G P  L+ED QA RPT + A PRVLN++YDK+  G+ AA  +K+ LF  A A K ++L  +G+L+  V+DRL+F K+++ +GLD +R +V+GSAP++  V+ F R++   P+ EGYGQTE  A  +++ P D  T+GHVG   P  E+ LVDVPEMGY HTD  H              QPC GRGEI  RGP +FKGYYK P KT E +D +GWLHSGD+G+WT +G L IIDRKKNIFKL+QGEYVA EKIENV++QS +I QAFV+GDSL+S LVA++VPD +  +        P     SF+E+C+   LK  I+ ++    +  GL GFEIPK+I+L  E F+  NGLLTPT KLKR QARD+Y   I+ +Y 
Sbjct:    8 STIVPQTSRKNERGNIRRGGDATRHPELGFKGCRTLYEGFRRGHNINPLGACLGFRAISTNGLATPYIYSSYTEVLARINAFAAGLDTLNLVPPTTKDDGEMILVGLYMKNCMEWFIAEQAIFCVSGATVPFYDTLGPESVQFILKQTSTKTVVSTRKELQRLCTVKKSGLCPYFEVVVLVDGVTA-HAAKIAKEAGLSVMSFAKVEAVGAHRIDTEGFQHRPPSGKDIFTFCYTSGTTGDPKGALLTHENMVSAIVGVGDIFPFET--TDRHLSYLPLAHIFERIIMAQIFMQGASIAFFRGDPLLLIEDFQACRPTVLAAAPRVLNKIYDKIQVGIAAAGGMKKKLFDAAVATKTKNLLTKGQLTHAVYDRLMFNKIKKGLGLDCLRSVVSGSAPLSKTVMNFYRIMLGIPVVEGYGQTENAACCTISLPEDMSTSGHVGIPNPAVEIVLVDVPEMGYRHTDTDH------------KGQPCQGRGEILARGPTVFKGYYKDPVKTEEAVDEEGWLHSGDIGLWTTDGNLQIIDRKKNIFKLSQGEYVAPEKIENVVIQSLLIGQAFVHGDSLQSALVAVIVPDEDPTRTLLENSEEPALAKASFSEICKSDKLKSIIMADLTKLGRANGLLGFEIPKSIHLSDEEFSVENGLLTPTFKLKRQQARDKYEKEIERMYT 672          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|299470304|emb|CBN78354.1| (long chain acyl-coA synthetase [Ectocarpus siliculosus])

HSP 1 Score: 608.986 bits (1569), Expect = 0.000e+0
Identity = 324/616 (52.60%), Postives = 415/616 (67.37%), Query Frame = 0

Query:   84 EAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQ------------------AAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVC-GPQELENLVAAAP--ECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCAGKALEVENFPD-DIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYA 677
            E   R+DN AS LE EGL PP PDG++LL LY +N PEWIIA+Q                  AAF     TVPLYDTLGP+ V +VI QT L  VVC G  EL  LVA A    CPSLK  V+   + S+  R  A + GL++Y+  ++E +G  +  PH PP P+D+ATF YTSGTTG PKGALLTHRNF+    A + +E  N    D++ SYLPLPH+FER++ +A+L  GAA+G ++G P  LVED+QALRPT M AVPRVLNR++DK++ G+ AA  LK  LF +A  AK++ L E G L+  +WD+LVF K++ A+GLDR+++MVTGSAP+A HVL FMR+L   P+ EGYGQTETTA +++T  GD++ GHVGG    +E+ L DVPEMGY HTD  HG   R  G  G+A +      EIC RG ++F GYYK  +KT E  D  GWL SGD+GMW  +G L IIDRKKNIFKL+QGEYVA EK+EN+  QS ++AQ++V+GDSL+S LVAI+VPD +A   WA       + S  ELCR   LK  ++ E+E  ++RAGL  FE  +AI+LE +PF+  NGLLTPT KLKR QA + YS +I  LYA
Sbjct:   39 EVEERVDNFASGLEQEGLTPPNPDGMKLLALYSRNRPEWIIAEQGGISYPSDSGTPIRILMKAAFAHSALTVPLYDTLGPETVEFVITQTSLTTVVCSGVAELRKLVAIADGGRCPSLKTVVVMDGI-SERERREAVRAGLRVYAFAEVEGIGASHAHPHRPPGPNDLATFCYTSGTTGNPKGALLTHRNFIADSAAAELMETFNADSTDVYLSYLPLPHIFERMVQVAILNVGAAVGIFRGDPTLLVEDMQALRPTVMAAVPRVLNRLHDKIVQGMLAAGGLKTKLFVKACEAKVQGLHE-GRLTHALWDKLVFGKIKAALGLDRIQMMVTGSAPVAAHVLTFMRILIGVPLLEGYGQTETTAGSTMTQSGDFSVGHVGGPFSCTEICLKDVPEMGYRHTDTWHGGDPRDAGGGGEACEGRG---EICFRGCNVFVGYYKDDEKTREAFDENGWLMSGDIGMWNLDGALKIIDRKKNIFKLSQGEYVAPEKLENIHTQSPLVAQSYVHGDSLRSSLVAIIVPDPDAAAGWADANGCG-NASMVELCRNPGLKADVMLELETVAERAGLQRFEKVRAIHLEPDPFSAQNGLLTPTFKLKRAQALEAYSKVIAALYA 648          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|1042761269|gb|ANQ45195.1| (long-chain acyl-CoA synthetase [Phaeodactylum tricornutum])

HSP 1 Score: 587.8 bits (1514), Expect = 0.000e+0
Identity = 311/681 (45.67%), Postives = 415/681 (60.94%), Query Frame = 0

Query:    7 RKKFYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAA--APECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGF----RYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCA--GKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDW-TTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDH-SFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYA 677
            ++   S  +P T+    G IRR  +  +N +L   GC T+YE FRR   + P  P +G R + + G A P+++ SY E   RID  A+ L+   L  P  DGL +L LYMKNC E  I +  AF + G++V +YDTLGPD V +++ QT   +VVC   EL  L  +  + +CP+  AA+L   V  D  + +A + GL++ S   +E +G          H PP P DVA F YTSGTTG PKGALLTH N ++      G   ++E  P D H SYLPL H+FER++   +   GA++ F++G P  L+ED+QA RPT MP  PRVLN++YDK+ AG+ +   LK+ LF  A AAK   L + G +   ++DRL+F K+++ +G+D++R+MV+GSAP+   V+ F R + A P+ EGYGQTE  A  ++    D  T GHVGG     E+ LVDVPEMGYLHTD  H    RG          C GRGEICVRGP +FKGYYK    T ETID +GWLHSGDVG+W P+G L IIDRKKNIFKL+QGEYVA EKIEN L+ S +I Q FVYGDS ++ LV ++VPD E  + WA   +  +   S AE+C    LK  ++ +++     + L GFE PK I L S PFT  N ++TPT KLKR + RD Y   I  LYA
Sbjct:    3 KQNMRSTILPQTDRDDRGEIRRSWNPVVNAELGVYGCRTMYEGFRRGAKVNPLGPCMGFRAVSTTGFATPFIYSSYTECLARIDAFAAGLDTLKLIQPNEDGLAVLALYMKNCMEGAIGEHGAFAIGGSSVLMYDTLGPDTVTFILDQTSARSVVCTRAELPKLCESKVSGKCPAFTAAILVDGVTEDAAK-MALEAGLEVLSFAKVEAVGAVCIAEKGHHHRPPAPTDVAAFCYTSGTTGNPKGALLTHENIMSAIAGFYGAVGDLEAQPFDRHLSYLPLAHIFERIVSSQMFCAGASVAFFRGDPTLLIEDMQACRPTVMPVAPRVLNKIYDKIQAGISSVGGLKKKLFDAAVAAKAEGL-QSGHMKHALYDRLIFNKIKKGLGMDQLRMMVSGSAPLNDTVMTFFRCMLAIPVVEGYGQTEGAAAATIGSSDDMATVGHVGGPVGSVEIVLVDVPEMGYLHTDTLH----RG--------MACQGRGEICVRGPSVFKGYYKDDLNTRETIDNEGWLHSGDVGLWRPDGNLQIIDRKKNIFKLSQGEYVAPEKIENFLISSPLIGQCFVYGDSYQNSLVGLMVPDEEPARTWAAVNAPELKSASLAEMCNSEALKAAVMADVQRIGVESRLLGFEKPKDILLVSVPFTIENEMMTPTFKLKRQKIRDTYEKEIDLLYA 669          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|219111209|ref|XP_002177356.1| (precursor of ligase long chain acyl-coa ligase [Phaeodactylum tricornutum CCAP 1055/1] >gi|217411891|gb|EEC51819.1| precursor of ligase long chain acyl-coa ligase [Phaeodactylum tricornutum CCAP 1055/1])

HSP 1 Score: 587.415 bits (1513), Expect = 0.000e+0
Identity = 312/681 (45.81%), Postives = 414/681 (60.79%), Query Frame = 0

Query:    7 RKKFYSIEVPNTETATAGPIRRFPDGEINPQLAYDGCYTIYELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDGLRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAA--APECPSLKAAVLTCSVLSDHHRALARKGGLKLYSLMDLEKLGF----RYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLTVYCA--GKALEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDW-TTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDH-SFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYA 677
             +   S  +P T+    G IRR  +  +N +L   GC T+YE FRR   + P  P +G R + + G A P+++ SY E   RID  A+ L+   L  P  DGL +L LYMKNC E  I +  AF + G++V +YDTLGPD V +++ QT   +VVC   EL  L  +  + +CP+  AA+L   V  D  + +A + GL++ S   +E +G          H PP P DVA F YTSGTTG PKGALLTH N ++      G   ++E  P D H SYLPL H+FER++   +   GA++ F++G P  L+ED+QA RPT MP  PRVLN++YDK+ AG+ +   LK+ LF  A AAK   L + G +   ++DRL+F K+++ +G+D++R+MV+GSAP+   V+ F R + A P+ EGYGQTE  A  ++    D  T GHVGG     E+ LVDVPEMGY HTD  H    RG          C GRGEICVRGP +FKGYYK    T ETID +GWLHSGDVG+W P+G L IIDRKKNIFKL+QGEYVA EKIEN L+ S +I Q FVYGDS ++ LV ++VPD E V+ WA   +  +   S AE+C    LK  ++ +I+     + L GFE PK I L S PFT  N ++TPT KLKR + RD Y   I  LYA
Sbjct:    3 NQNMRSTVLPQTDRHDRGEIRRSWNPVVNAELGVYGCRTMYEGFRRGAKVNPLGPCMGFRAVSTTGFATPFIYSSYTECLARIDAFAAGLDTLKLIQPNEDGLAVLALYMKNCMEGAIGEHGAFAIGGSSVLMYDTLGPDTVTFILDQTSARSVVCTRAELPKLCESKVSGKCPAFTAAILVDGVTEDAAK-MALEAGLEVLSFAKVEAVGAVCIAEKGHHHRPPAPTDVAAFCYTSGTTGNPKGALLTHENIMSAIAGFYGAVGDLEAQPFDRHLSYLPLAHIFERIVSSQMFCAGASVAFFRGDPTLLIEDMQACRPTVMPVAPRVLNKIYDKIQAGISSVGGLKKKLFDAAVAAKAEGL-QSGHMKHALYDRLIFNKIKKGLGMDQLRMMVSGSAPLNDTVMTFFRCMLAIPVVEGYGQTEGAAAATIGSSDDMATVGHVGGPVGSVEIVLVDVPEMGYFHTDTLH----RG--------MACQGRGEICVRGPSVFKGYYKDDLNTRETIDNEGWLHSGDVGLWRPDGNLQIIDRKKNIFKLSQGEYVAPEKIENFLISSPLIGQCFVYGDSYQNSLVGLMVPDEEPVRTWAAVNAPELKSASLAEMCNSEALKAAVMADIQRIGVESRLLGFEKPKDILLVSVPFTIENEMMTPTFKLKRQKIRDTYEKEIDLLYA 669          

BLAST of EWM20588.1 vs. NCBI_GenBank
Match: gi|294893726|ref|XP_002774616.1| (Long-chain-fatty-acid--CoA ligase, putative [Perkinsus marinus ATCC 50983] >gi|239880009|gb|EER06432.1| Long-chain-fatty-acid--CoA ligase, putative [Perkinsus marinus ATCC 50983])

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 314/693 (45.31%), Postives = 426/693 (61.47%), Query Frame = 0

Query:   11 YSIEVPNTET-ATAGPIRRFPDGEINPQLAYDGCYTI---YELFRRSVAIYPHRPFLGHRPIDSEGHAAPYVFESYEEAARRIDNLASALEHEGLCPPTPDG-LRLLGLYMKNCPEWIIADQAAFTLHGATVPLYDTLGPDAVGYVIKQTELAAVVCGPQELENLVAAAPECPSLKAAV-LTCSVLSDHHRALARKGGLKLYSLMDLEKLGFRYPQPHFPPDPDDVATFSYTSGTTGGPKGALLTHRNFLT------VYCAGKA--LEVENFPDDIHFSYLPLPHVFERVMLLAVLGNGAAMGFWQGSPERLVEDLQALRPTCMPAVPRVLNRMYDKVMAGVRAASPLKQMLFQRAYAAKLRDLDERGELSKGVWDRLVFAKVRRAIGLDRMRVMVTGSAPIAPHVLQFMRVLCACPIHEGYGQTETTAMTSLTFPGDWTTGHVGGVAPVSEVRLVDVPEMGYLHTDRQHGSAGRGGGAFGKAAQPCIGRGEICVRGPHIFKGYYKQPDKTAETIDAQGWLHSGDVGMWTPEGRLVIIDRKKNIFKLAQGEYVAAEKIENVLMQSEMIAQAFVYGDSLKSELVAIVVPDAEAVQDWARQESLPVDHSFAELCRQSLLKEKILDEIEVQSKRAGLHGFEIPKAIYLESEPFTGANGLLTPTQKLKRMQARDRYSTIIQELYATRDLHQGSKARL 689
            Y+ EVP++     +GPI R  D         DG  ++   + +FR++   Y  +P  G RP   +G   PY +++Y+E   RI N    LEH  L   + DG +R++G+YMKN  EWIIA+Q AFT    TVPLYDTLG D V Y++ Q  L  VVC   ++  LVA   ECPSL   + +     S+       + GLKL+S +D+E  G ++       DP D+ATF YTSGTTG PKGAL +H N +       V+   K   +++       + SYLPLPH+ ER++ ++++  G A+GF+QG P +++ED+ ALRPT   +VPR+LNR+YDK+  G RAA  +K  LF+ A AAK   L E+G L   +WD+LVF K++  IGLDR++++ +GSAP+A HV+ F+RV   C + EGYGQTE T + + + P D+TTGHVG      +++L DVPEMGYL TD QHGS        G AA  C GRGEIC RGP +F+GYYK PDKT E ID  GWLHSGDVG+W   GRL IIDRKK+IFKL+QGEYV+AE++ENV+ QS +IAQAFV+G+S  S LVA++VPD E ++     ++   D   A LC+   + + ILD +   S    L GFE+ KAI+L  EPFT  N LLTP+ KLKR  A  R+   + ++Y       G K  L
Sbjct:   13 YAYEVPDSAIPGKSGPIYRNVDAAEGNLSVIDGDESLDSSFAIFRKAAEKYADQPCHGTRPRLDDGSVGPYEWQTYKEVYDRILNFGRGLEHLNLLSKSGDGSMRVIGIYMKNRSEWIIAEQGAFTRRATTVPLYDTLGEDVVDYIVNQVVLETVVCTASQIPRLVACKAECPSLTNIICVDPEACSESVTTAMTEAGLKLHSFIDVENTGSKFDDEPLEGDPTDIATFCYTSGTTGDPKGALNSHGNIVANVSSFIVFLGTKTPIMDIRG-SQQYYLSYLPLPHIMERLISISLVHLGVAVGFYQGEPLKIMEDIAALRPTIFVSVPRLLNRLYDKITLGARAAGGIKSYLFEHAMAAKKARL-EQGILKHPLWDKLVFDKIKARIGLDRVQLVASGSAPLATHVMDFLRVFFGCAVAEGYGQTECTCLATYSHPHDFTTGHVGQPVCCMDIKLFDVPEMGYLSTDTQHGS--------GDAAIECKGRGEICFRGPSVFQGYYKMPDKTEEAIDEDGWLHSGDVGLWLTTGRLKIIDRKKSIFKLSQGEYVSAERVENVITQSRLIAQAFVFGNSFHSCLVAVIVPDFEMLESIPGIQAGKTDP--ASLCKNEQVNKAILDSVREYSIAERLRGFEMVKAIHLIPEPFTVENDLLTPSFKLKRNVASARFQKEVDDMYIGIGDKVGGKQNL 693