EWM20663.1, cds10010 (CDS) Nannochloropsis gaditana

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|585100468|gb|EWM20663.1| (dna topoisomerase 3-beta-1 [Nannochloropsis gaditana])

HSP 1 Score: 1782.69 bits (4616), Expect = 0.000e+0
Identity = 858/858 (100.00%), Postives = 858/858 (100.00%), Query Frame = 0

Query:    1 MHSSLVIESASDRRSLASLHKVLCCPIFSIGRSPPVHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKHVAIARKDFAKRNRGRGRGRGRGRGRGGKGGANPLMSFEDF 858
            MHSSLVIESASDRRSLASLHKVLCCPIFSIGRSPPVHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKHVAIARKDFAKRNRGRGRGRGRGRGRGGKGGANPLMSFEDF
Sbjct:    1 MHSSLVIESASDRRSLASLHKVLCCPIFSIGRSPPVHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKHVAIARKDFAKRNRGRGRGRGRGRGRGGKGGANPLMSFEDF 858          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|553192648|ref|XP_005855616.1| (DNA topoisomerase III [Nannochloropsis gaditana CCMP526] >gi|422293447|gb|EKU20747.1| DNA topoisomerase III [Nannochloropsis gaditana CCMP526])

HSP 1 Score: 1696.79 bits (4393), Expect = 0.000e+0
Identity = 824/845 (97.51%), Postives = 826/845 (97.75%), Query Frame = 0

Query:   31 GRSPPVHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNP-------LGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFK--GGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLS--------GGETKIEGCVLCHDILNTCTELAKGRHKHVAIARKDFAKRNRGRGRGRGRGRGRGGKGGANPLMSFEDF 858
            GRSPPVHEF+GRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQ GFKAHTRILRAHFSAITPKDIHRALQ LGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNP       LGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFK  GGNIH+IKPATSQYCEVCGSAVLTIEYQKDKSPLS        GGETKIEGCVLCHDILNTCTELAKGRHKHVAIARKDFAKRNRGRGRGRGRGRGRGGKGGANPLMSFEDF
Sbjct:   26 GRSPPVHEFHGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQPGFKAHTRILRAHFSAITPKDIHRALQKLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGDCGNILGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFKVTGGNIHSIKPATSQYCEVCGSAVLTIEYQKDKSPLSGEEGRFCAGGETKIEGCVLCHDILNTCTELAKGRHKHVAIARKDFAKRNRGRGRGRGRGRGRGGKGGANPLMSFEDF 870          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|260805074|ref|XP_002597412.1| (hypothetical protein BRAFLDRAFT_80591 [Branchiostoma floridae] >gi|229282677|gb|EEN53424.1| hypothetical protein BRAFLDRAFT_80591 [Branchiostoma floridae])

HSP 1 Score: 897.116 bits (2317), Expect = 0.000e+0
Identity = 436/800 (54.50%), Postives = 560/800 (70.00%), Query Frame = 0

Query:   36 VHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKE---PTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKA----HTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVA-KDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQS--KDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGL-SIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACP-GTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKHVAIAR 823
            VHE+ G+F G+S + ++TSV GHV S DF G Y+NW+ VDP DLF+APT K+   P  K +   L  EG+GADILVLWLDCD+EGENICFEVI+ ++            I R+ FSAIT  DI  A+  LG PN++EA+SVDARQELDL++G AF+RFQT++FQGKYGDLD++L+S+GPCQTPTLGFCV RHD+IQSF PE +W +   V+ K+ + + L+W R R+FD EVA  FL++V  A    V  V K E+ + +P ALNTVELL+ AS  LGM P  TM VAERLY  GY+SYPRTE+T YPE+FD++G L  Q  + +WG   + LL +G+ +PR G DAGDHPPITP+R+   +E  G+  R+Y+ +T HF+A++S D ++LQT V F    GESFS +GK +ID GY AV+       E  +P F++GD  T + + L +  TSPP YLTE+ELI  MEKHGIGTDASI  HINNI +RNYV VG GR LIP PLGIVLVHGY +ID +LVLP +R+ +E++  LIA+G+A+  +V+ H+L  F  KF YF + +  MD LFE TFSPLAA+GKPLSRCGKCRRFMKY+  KP RL+C  C+ETY LPQ GT+KLYKE+KCPLDDFEL+LFS     G++GK++PLCPYC++HPPF  +   MGC+ C HPTC HSL  NGV  C       C  G LV+D  S P WKMACNRC N+V+    N H +  + S+ CE CG++++ +++ K KSPL GGET+  GC+ C  IL    E      KH A  R
Sbjct:   38 VHEYTGKFHGESVKFKMTSVCGHVMSLDFQGVYNNWDRVDPADLFRAPTNKKEANPNLK-MPKFLELEGRGADILVLWLDCDKEGENICFEVIDAVRPSMNRPRGREQYIYRSKFSAITETDIRGAMMRLGEPNENEAMSVDARQELDLRIGCAFTRFQTKFFQGKYGDLDSSLISFGPCQTPTLGFCVDRHDQIQSFKPETYWVIQTQVSGKNNSPLWLDWDRVRLFDREVAQTFLNIVKGAKEAKVVSVTKKEKAKQRPIALNTVELLRVASSSLGMAPQHTMQVAERLYTQGYISYPRTETTHYPENFDLKGTLRQQTGNSSWGPIVRELLDKGMQKPRKGSDAGDHPPITPMRSATESELGGEGWRVYEYITSHFIATVSKDCKYLQTTVKFDV-AGESFSVTGKTLIDAGYTAVMHWQGISAEESVPHFEKGDVCTVNEVKLAEKQTSPPDYLTEAELISLMEKHGIGTDASIPVHINNICQRNYVTVGGGRKLIPTPLGIVLVHGYQKIDPELVLPHMRSAVETQLNLIAQGKANYNAVLQHALDIFRLKFHYFVDSVSDMDQLFEVTFSPLAASGKPLSRCGKCRRFMKYVQAKPSRLHCATCDETYNLPQGGTVKLYKEIKCPLDDFELVLFS----TGSKGKTYPLCPYCFNHPPFPDMKKAMGCNQCTHPTCTHSLINNGVAQC-----VECEDGVLVMDQTSAPKWKMACNRC-NVVINMFENAHKVNVSASEDCEECGASIIDVDFNKAKSPLEGGETQHSGCIFCDPILTPLIE------KHYAATR 819          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|1126179299|ref|XP_019622045.1| (PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 3-beta-1-like [Branchiostoma belcheri])

HSP 1 Score: 890.182 bits (2299), Expect = 0.000e+0
Identity = 432/796 (54.27%), Postives = 561/796 (70.48%), Query Frame = 0

Query:   36 VHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKE---PTSKFITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKA----HTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVA-KDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVL---AQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGL-SIMGCDSCMHPTCKHSLTRNGVCPCPGESGAACP-GTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTE--LAKGRH 816
            VHE+ G+F G+  + ++TSV GHV S DF G Y+NW+ VDP DLF+APT K+   P  K +   L  E +GADIL+LWLDCD+EGENICFEVI+ ++            I R+ FSAIT  DI  A+  LG PN++EA+SVDARQELDL++G AF+RFQT++FQGKYGDLD++L+S+GPCQTPTLGFCV RHD+IQSF PE +W +   V+ K+ + + L+W R R+FD EVA  FL++V  A    V  V K E+ + +P ALNTVELL+ AS  LGM P  TM VAERLY  GY+SYPRTE+T YPE+FD++G L  Q  + +WG   + LL +G+ +PR G DAGDHPPITP+++   +E  G+  RLY+ +T HF+A+LS D ++LQT V F +  GESFS +GK +ID GY AV+   A S +ES +P F++GD  T + + L +  TSPP YLTE+ELI  MEKHGIGTDASI  HINNI +RNYV VG GR LIP PLGIVLVHGY +ID +LVLP +R+ +E++  LIA+G+A+  +V+ H+L  F  KF YF + I  MD LFE +FSPLAA+GKPLSRCGKCRRFMKY+  KP RL+C  C+ETY LPQ GT+KLYKE+KCPLD+FEL+LFS     G++GK++PLCPYC++HPPF  +   MGC+ C HPTC  SL  NGV  C       C  G LV+D  S P WKMACN+C N+V+    N H +  + S+ CE CG++++ +++ K KSPL GGET+  GC+ C  +L    E   A  RH
Sbjct:   38 VHEYTGKFHGEMVKFKMTSVCGHVMSLDFQGVYNNWDRVDPADLFRAPTNKKEANPNLK-MPKFLELEARGADILILWLDCDKEGENICFEVIDAVRPSMNRPRGREQYIYRSKFSAITETDIRGAMMRLGEPNENEAMSVDARQELDLRIGCAFTRFQTKFFQGKYGDLDSSLISFGPCQTPTLGFCVDRHDQIQSFKPETYWVIQLQVSGKNNSPIWLDWERVRLFDREVAQTFLNIVKAAKEARVVSVTKKEKAKQRPIALNTVELLRVASSSLGMAPQHTMQVAERLYTQGYISYPRTETTHYPENFDLKGTLRQQTGNSSWGPIVRELLDKGIQKPRKGADAGDHPPITPMKSATESELGGEGWRLYEYITSHFIATLSKDCKYLQTTVRFDA-AGESFSVTGKTLIDAGYTAVMHWQAISAEES-VPHFEKGDTCTVNEVKLAEKQTSPPDYLTEAELISLMEKHGIGTDASIPVHINNICQRNYVTVGGGRKLIPTPLGIVLVHGYQKIDPELVLPHMRSAVETQLNLIAQGKANFHAVLQHALDIFRLKFHYFVDSISDMDQLFEVSFSPLAASGKPLSRCGKCRRFMKYVQAKPSRLHCATCDETYNLPQGGTVKLYKEIKCPLDEFELVLFS----TGSKGKTYPLCPYCFNHPPFPDMKKAMGCNQCTHPTCTQSLINNGVAQC-----VECEDGVLVMDQTSAPKWKMACNKC-NVVINVFENAHKVSVSASEDCEECGASIIDVDFNKAKSPLEGGETQHSGCIFCDPLLTPLIERHYAATRH 820          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|1083426578|ref|XP_018584258.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Scleropages formosus] >gi|1083426614|ref|XP_018584259.1| PREDICTED: DNA topoisomerase 3-beta-1 [Scleropages formosus] >gi|1083426678|ref|XP_018584260.1| PREDICTED: DNA topoisomerase 3-beta-1 [Scleropages formosus] >gi|1083426719|ref|XP_018584261.1| PREDICTED: DNA topoisomerase 3-beta-1 [Scleropages formosus])

HSP 1 Score: 880.167 bits (2273), Expect = 0.000e+0
Identity = 437/827 (52.84%), Postives = 566/827 (68.44%), Query Frame = 0

Query:    5 LVIESASDRRSLAS-LHKVLCCPIFSIGRSPPVHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLF-KAPT-RKEPTSKF-ITHHLASEGKGADILVLWLDCDREGENICFEVIECMQ----SGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKD-GANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQ--SKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSI-MGCDSCMHPTCKHSLTRNGVCPCPGESGAACP-GTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKH 818
            +V E  S  +S+A  L K  C        +  VHE+ G F GQS R ++TSV GHV S DF G+Y+NW+ VDP +LF KAPT +KE   K  +   L  E KG DI+VLWLDCD+EGENICFEV++ +Q      +     + RA FS+IT  DI  A+  LG PN++EALSVDARQELDL++G AF+RFQT+YFQGKYG+LD++L+S+GPCQTPTLGFCV+RHD+IQSF PE +W + A V KD  + V L+W R RVFD EV  +F+++   +    VD V K E+ + +P ALNTVE+L+ AS  LGMGP   M +AERLY  GY+SYPRTE+T YPE+FD++G L  Q ++P WGD  K LL EGV+RPR G DAGDHPPITP+RA   +E   D  RLY+ +TRHF+A++SHD ++LQT + F+    E+FS +GK +I  G+  V+      DE  LP  ++GD FT   + L +  T PP YLTESELI  MEKHGIGTDASI  HINNI +RNYV V  GR L P  LGIVLVHGY +IDA+LVLP +R+ +E +  LIA+G+A+ Q V+ H+L  F+ KF YF + I  MD L E +FSP+AATGKPLSRCGKC RFMKYI  KP RL+C  C+ETY+LPQNG IKLYKEL+CPLDDFEL+L++     G++GKS+PLCPYC+S+PPF  +   MGC+ C HP+C+HSL+  G+  C       C  G LV D  S P WKMACNRCN +V F   + H ++  ++  CE C ++++ +++ K +SPL G ET+  GCV C  +     EL     +H
Sbjct:    6 MVAEKPSLAQSIAKILSKGSCSTRKGFNGACSVHEYTGIFMGQSVRFKMTSVCGHVMSLDFTGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEAKGCDIVVLWLDCDKEGENICFEVLDAIQPVMNKAYSGERSVYRAKFSSITDTDICNAMNRLGEPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVIQAKVFKDKDSPVMLDWERVRVFDREVGQMFVNIAKASREAWVDSVNKKEKAKQRPLALNTVEMLRVASAALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGTLRQQTNNPFWGDAVKTLLAEGVNRPRKGTDAGDHPPITPMRAASESELGSDGWRLYEYITRHFIATVSHDCKYLQTTISFTIG-SEAFSCTGKTLISAGFTEVMPWLGIVDEESLPTCEKGDSFTVEEVKLVEKQTGPPDYLTESELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKANFQQVLQHTLDIFKRKFHYFVDSIASMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELRCPLDDFELVLWT----SGSRGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLSSLGIGQC-----VECENGVLVFDPTSGPKWKMACNRCNVVVNF-FEHAHRVQ-VSADSCEACEASLVVVDFNKARSPLPGDETQHSGCVFCDPVFQDLVELKHATMRH 820          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|919065867|ref|XP_013414482.1| (PREDICTED: DNA topoisomerase 3-beta-1-like [Lingula anatina])

HSP 1 Score: 877.085 bits (2265), Expect = 0.000e+0
Identity = 426/782 (54.48%), Postives = 555/782 (70.97%), Query Frame = 0

Query:   36 VHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPTRKEPTSK--FITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGF---KAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAK-DGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECG-ESFSASGKIMIDPGYLAVL---AQSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSI-MGCDSCMHPTCKHSLTRNGVCPCPGESGAACP-GTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDIL 805
            +HE+ G+F G++ R ++TSV GHV S DF G+Y+NW+ VDP +LF APT+K+  S    +   L  E +G D LVLWLDCD+EGENICFEVI+ +Q      +    I RAHFSAIT  DI RA+ NLG PNK+EA+SVDARQELDL++G AF+R+QT++FQGKYGDLD+TL+SYGPCQTPTLGFCV+RHD+IQSF PE FW +   V    G  + L+W R R+FD EVA +FL++V  A    V  +   E+ + +P ALNTVELL+  S  LGMGP  TM +AE+LY  GY+SYPRTE+TAYPE+FD++G L  Q++   WG   + LL++G+++P+ G DAGDHPPITP+R    +E   +  RLYD +TRHF+A++S D R+LQT   F  E G E F+ +GK ++  G+ AV+   A + DE+ +P+FQ     +   + L +  TSPP YLTESELI  MEKHGIGTDASI  HINNI ERNYV V  GR L P PLGIVLVHGY +ID DLVLP +R+ +E + TLIA G+A+  +V+ H+L+ FE KF+YF ++I  MD LFE TFSPLAA+GKPLSRCGKCRR+MK++  KP RL+C  C+ET++LPQNG+IKLYKE+KCPLDDFE++L++     G++GKS+PLCPYCY+HPPF  +   MGC+ C HPTC H+L +NGV  C       C  G LVLD +S P W+MACN+C N+V+  G NIH I   +   CE C + +L  ++ K+KSPL  G  +  GCV C  IL
Sbjct:   38 IHEYNGKFLGETVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFHAPTQKKEASVNLHMPKFLEKEARGCDYLVLWLDCDKEGENICFEVIDSVQHVMNRSRGDQTIYRAHFSAITEADIKRAMANLGSPNKNEAMSVDARQELDLRIGCAFTRYQTKFFQGKYGDLDSTLISYGPCQTPTLGFCVERHDKIQSFKPEPFWVIQLPVTHPSGRTLHLDWDRVRIFDKEVAQMFLNIVKGADKARVVNIVNKEKTKARPQALNTVELLRVGSSGLGMGPQHTMQIAEKLYTQGYISYPRTETTAYPENFDLKGTLRQQMNSSDWGKEVRELLEQGINKPKKGTDAGDHPPITPMRPATESELGHEAWRLYDYITRHFIATVSPDCRYLQTTAYF--EVGAEKFTCTGKTLLSAGFTAVMTWQAITGDEN-MPNFQVDQTCSIEEVRLVEKQTSPPDYLTESELITLMEKHGIGTDASIPVHINNISERNYVSVIGGRKLRPTPLGIVLVHGYQKIDPDLVLPTMRSAVEQQLTLIAHGRANFDAVLQHALKIFEMKFKYFVQNILGMDELFEVTFSPLAASGKPLSRCGKCRRYMKFVQAKPSRLHCSQCDETFSLPQNGSIKLYKEIKCPLDDFEMVLWT----TGSKGKSYPLCPYCYNHPPFRDMRKGMGCNECTHPTCPHALGQNGVASC-----VECENGVLVLDQSSAPKWRMACNKC-NIVINMGENIHKIA-LSDDTCEDCDATILDFDFNKEKSPLEDGAIEHSGCVFCDPIL 805          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|632980244|ref|XP_007906923.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Callorhinchus milii])

HSP 1 Score: 876.315 bits (2263), Expect = 0.000e+0
Identity = 426/794 (53.65%), Postives = 552/794 (69.52%), Query Frame = 0

Query:   36 VHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLF-KAPT-RKEPTSKF-ITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKA----HTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVA-KDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLA-QSKDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSI-MGCDSCMHPTCKHSLTRNGVCPCPGESGAAC-PGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKH 818
            VHE  G F+GQS   +VTSV GHV + DF G+Y+NW+ VDP +LF KAPT +KE   K  +   L  EG+G D +VLWLDCD+EGENICFEV++ +Q            + RA FSAIT  DI+ A+  LG PN++EALSVDARQELDL+VG AF+RFQT+YFQGKYG+LD++L+S+GPCQTPTLGFCV+RHD+IQSF PE +W L A V+  +G ++ L+W R RVFD EVA ++L++   A    V+ V K E+ + +P ALNTVE+L+ AS  LGMGP  TM +AE+LY  GY+SYPRTE+T YPESFD++G L  Q ++P W D  K LL +G++RPR G D GDHPPITP+R+   +E   D  RLY+ +TRHF+A++S D ++LQT + F     E F+ SGK +I PGY  V+  Q+ +E  LP    GD FT   + L +  T PP YLTE+ELI  MEKHGIGTDASI  HINN+ +RNYV V  GR L P  LGIVLVHGY +ID +LVLP +R+ +E + +LIA G+ D   V++H+L  F+ KF YF + I  MD L E +FSP+A TGKPLSRCGKC RFMKYI  KP R++C  C+ETY+LPQNGTIKLYKEL+CPLD+FEL+L+S     G++GKS+PLCPYCY+HPPF  +   MGC+ C HPTC+HSL+  G+  C       C  G LVLD  S P WKMACN+CN +V F   N H +K  +S+ CE+C +A++ +++ K K+PL    T+  GCV C  +     EL   + KH
Sbjct:   38 VHEHTGTFQGQSVHFKVTSVCGHVMTLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVRFLQVEGRGIDYIVLWLDCDKEGENICFEVLDVVQPVMNVSRGNERTVYRAKFSAITDTDINNAMNRLGEPNRNEALSVDARQELDLRVGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPEAYWVLQAKVSDSEGNSIILDWDRVRVFDREVAQMYLNMTKTARETKVECVSKKEKVKQRPQALNTVEMLRVASSALGMGPQHTMQIAEKLYTQGYISYPRTETTQYPESFDLKGTLRQQANNPFWSDVVKALLTDGINRPRKGQDVGDHPPITPMRSASESELGSDGWRLYEYITRHFIATISPDCKYLQTTISFCLN-EEHFTCSGKTLISPGYTLVMPWQNMEEEILPVCARGDVFTMDEIKLLEKQTHPPDYLTEAELITLMEKHGIGTDASIPVHINNVCQRNYVTVESGRRLKPTNLGIVLVHGYYKIDQELVLPTIRSAVEKQLSLIAMGKVDFSQVLEHALNIFKRKFHYFVDSIAGMDELMEVSFSPIAETGKPLSRCGKCHRFMKYIQAKPSRMHCSHCDETYSLPQNGTIKLYKELRCPLDEFELVLWS----SGSRGKSYPLCPYCYNHPPFRDMRKGMGCNECTHPTCQHSLSLLGIVQC-----VECESGVLVLDSTSGPKWKMACNKCNVVVHF-FENAHRVK-VSSETCEICEAAIVDVDFNKTKTPLPDEATQHSGCVFCDPVFQNLVELQYAKMKH 819          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|762120228|ref|XP_011443037.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Crassostrea gigas])

HSP 1 Score: 874.004 bits (2257), Expect = 0.000e+0
Identity = 428/798 (53.63%), Postives = 554/798 (69.42%), Query Frame = 0

Query:   36 VHEFYGRF--EGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLFKAPT-RKEPTSKF-ITHHLASEGKGADILVLWLDCDREGENICFEVIECMQSGFKAHTR--ILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVA-KDGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECG-ESFSASGKIMIDPGYLAVLAQS--KDESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGL-SIMGCDSCMHPTCKHSLTRNGVCPCPGESGAAC-PGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKHVAI 821
            +HE+ G+F   G+S   ++TSV GHVF  DF G+Y++W+ VDPV+LF APT +KE T K  + + L  E +G D LVLWLDCD+EGENICFEVI+C +      +   + RA FSAIT KDI  A+  L  PNK+EALSVDARQELDL++G AF+RFQT+YFQGKYGDLD+TL+SYGPCQTPTLG CV RHD+IQSF PE +W +   +A  +G  V ++W R RVFD EVA +F++++  + S  V KV +  + + +P ALNTVE+L+ AS  LGMGP   M +AERLY+ GY+SYPRTE+T YPE+FD+ G L  Q S   WGDY + LL+EG+ +P+ G D GDHPPITP+R     E   D  RLYD +TRHF+ ++S D ++LQT   F  E G E F+ +G  + DPG+ AV++    +DE  LP+F++   +  + + + + HTSPP YLTESELI  MEKHGIGTDASI  HINNI ERNYV V  GR L P PLGIVLVHGY +ID +LVLP +R+ +E +  LIA G+A  + V+ H+L  F  KF+YF E+I  MD LFE TFSPLAATGKPLSRCGKCRR+MKY+  KP RL+C  C+ETY+LPQNG IKLYKELKCPLDDFEL+L++     G +GKSFPLCPYC+++PPF  +    GC+ C HPTC+H+L ++GVC C       C  G LVLD  SYP W+MACN+C  ++ F   N H +   + + C+ CG+ ++ +++ K+K+PL   ETK  GCV C  I     E      KH AI
Sbjct:   38 IHEYKGKFPPTGESVSYKMTSVCGHVFGLDFTGKYNSWDRVDPVELFTAPTLKKEATEKLRMPNFLEKEARGVDYLVLWLDCDKEGENICFEVIDCAKPSMNRFSDQCVYRAKFSAITDKDIKSAMTKLIAPNKNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSTLISYGPCQTPTLGLCVDRHDKIQSFKPEPYWVIQVKLALPEGRTVDIDWDRIRVFDKEVAQMFVNMIKSSDSARVLKVTQDVKSKARPQALNTVEMLRAASSGLGMGPQHAMQMAERLYIQGYISYPRTETTQYPENFDLVGTLRQQASSSMWGDYVQNLLREGIQKPKKGTDVGDHPPITPMRPATEAELGHDAWRLYDFITRHFIGTISPDCKYLQTTAFF--EIGAERFTCTGYRVTDPGFTAVMSWKSLEDEWTLPEFKKDMDYNITEVKMVEKHTSPPDYLTESELISLMEKHGIGTDASIPVHINNICERNYVTVTSGRRLKPTPLGIVLVHGYQKIDIELVLPTMRSAVEKQLNLIAAGKAYFEDVLKHALNIFCLKFKYFVENIGGMDELFEVTFSPLAATGKPLSRCGKCRRYMKYVQAKPSRLHCSNCDETYSLPQNGNIKLYKELKCPLDDFELVLWT----TGVRGKSFPLCPYCFNNPPFPDMRKQFGCNQCTHPTCQHALIQHGVCEC-----VECETGILVLDPTSYPKWRMACNKCQVVINF-FENAHKVS-VSEELCDDCGAHIVDVDFSKEKTPLPNKETKHSGCVFCDSIFKNLVE------KHHAI 816          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|1079800388|ref|XP_018525113.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Lates calcarifer])

HSP 1 Score: 872.848 bits (2254), Expect = 0.000e+0
Identity = 434/826 (52.54%), Postives = 565/826 (68.40%), Query Frame = 0

Query:    5 LVIESASDRRSLAS-LHKVLCCPIFSIGRSPPVHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLF-KAPT-RKEPTSKF-ITHHLASEGKGADILVLWLDCDREGENICFEVIECMQ----SGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAK-DGANVALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFSSECGESFSASGKIMIDPGYLAVLAQSKD--ESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSI-MGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKH 818
            +V E  S  +S++  L K  C     +  +  VHE+ G F+GQ+ R ++TSV GHV S DF G+Y+NW+ VDP +LF KAPT +KE T K  +   L  E +G D +VLWLDCD+EGENICFEV++ +Q     G      + RA FS+IT  DI  A+  LG PN++EALSVDARQELDL++G AF+RFQT+YFQGKYG+LD++L+S+GPCQTPTLGFCV+RHD+IQSF PE +W L A V K   + + L+W R RVFD EV  +F++L   +    V  V K E+ + +P ALNTVE+L+ AS  LGMGP  TM +AERLY  GY+SYPRTE+T YPE+FD++G L  Q ++P W D  K LL  G++RPR G DAGDHPPITP+RA    E   D  RLY+ +TRHF+A++S D ++LQT + FS    E+FS SGK +I PGY AV+       E  LPD + GD FT   + L +  TSPP YLTE+ELI  MEKHGIGTDASI  HINNI +RNYV + +GR L P  LGIVLVHGY +IDA+LVLP +R+ +E +  LIA G+A+ Q V+ H+L  F+ KF YF + I  MD L E +FSP+AATGKPLSRCGKC RFMKYI  KP RL+C  C+ETY+LPQNG IKLYKEL+CPLDDFEL+L++     GA+GKS+PLCPYC+S+PPF  +   MGC+ C HP+C+HSL   G+    G+      G LVLD  S P W+MACN+CN +V F   + H ++ A  + C+ C ++++ +++ K ++PL  GET+  GCV C  I     EL     +H
Sbjct:    6 MVAEKPSLAQSISKILSKGNCTSRKGLNGACSVHEYTGSFQGQTVRFKMTSVCGHVMSLDFIGKYNNWDKVDPAELFSKAPTEKKEATPKLNMVKFLQVEARGCDYVVLWLDCDKEGENICFEVLDAIQPVMNKGSVRERSVYRAKFSSITDTDIWNAMNCLGEPNRNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWILQAKVFKGKDSPLTLDWNRVRVFDREVGQMFVNLAKTSREAQVQSVSKKEKTKQRPQALNTVEMLRVASSALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGTLKQQTNNPIWADEVKALLSTGLNRPRKGTDAGDHPPITPMRAASEGELGSDGWRLYEYITRHFIATVSQDCKYLQTTIDFSIG-TETFSCSGKTLISPGYTAVMPWQGIPLEESLPDCECGDTFTVDEIKLVEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTIENGRKLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIALGKANYQQVLQHALDIFKRKFHYFVDSITSMDELMEVSFSPIAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGAIKLYKELRCPLDDFELVLWT----SGARGKSYPLCPYCFSNPPFRDMKKGMGCNECTHPSCQHSLNSLGI----GQCVECDSGVLVLDPTSGPKWRMACNKCNVVVHF-FEHAHRVQVA-QESCDACDASLVAVDFNKTRTPLPAGETQHTGCVFCDPIFQDLVELKHATMRH 820          

BLAST of EWM20663.1 vs. NCBI_GenBank
Match: gi|975099458|ref|XP_015264778.1| (PREDICTED: DNA topoisomerase 3-beta-1 [Gekko japonicus])

HSP 1 Score: 872.463 bits (2253), Expect = 0.000e+0
Identity = 430/796 (54.02%), Postives = 551/796 (69.22%), Query Frame = 0

Query:   36 VHEFYGRFEGQSARIRVTSVTGHVFSTDFPGQYSNWEAVDPVDLF-KAPT-RKEPTSKF-ITHHLASEGKGADILVLWLDCDREGENICFEVIEC----MQSGFKAHTRILRAHFSAITPKDIHRALQNLGVPNKDEALSVDARQELDLKVGVAFSRFQTRYFQGKYGDLDATLVSYGPCQTPTLGFCVQRHDEIQSFSPEKFWYLAAAVAKDGAN-VALEWGRGRVFDHEVAHVFLHLVGEAGSVAVDKVQKSEQRRTKPTALNTVELLKEASKVLGMGPAQTMSVAERLYLSGYLSYPRTESTAYPESFDVRGALSLQVSHPTWGDYAKGLLQEGVSRPRAGVDAGDHPPITPVRALDPTEFPGDMGRLYDLVTRHFLASLSHDARFLQTKVLFS--SECGESFSASGKIMIDPGYLAVLAQSK--DESKLPDFQEGDHFTFSSLGLGDGHTSPPGYLTESELIERMEKHGIGTDASISSHINNIVERNYVHVGDGRTLIPNPLGIVLVHGYLRIDADLVLPKVRAQIESECTLIAKGQADMQSVVDHSLRNFEAKFRYFAEHIDLMDSLFEATFSPLAATGKPLSRCGKCRRFMKYIHLKPQRLYCPICNETYALPQNGTIKLYKELKCPLDDFELLLFSLGNAQGAQGKSFPLCPYCYSHPPFEGLSI-MGCDSCMHPTCKHSLTRNGVCPCPGESGAACPGTLVLDVNSYPNWKMACNRCNNLVLFKGGNIHTIKPATSQYCEVCGSAVLTIEYQKDKSPLSGGETKIEGCVLCHDILNTCTELAKGRHKH 818
            VHE+ G F GQS R ++TSV GHV + DF G+Y+NW+ VDP +LF KAPT +KE   K  +   L  EG+  D +VLWLDCD+EGENICFEV++     M         + RA FS+IT  DI  A+ NLG PN +EALSVDARQELDL++G AF+RFQT+YFQGKYG+LD++L+S+GPCQTPTLGFCV+RHD+IQSF PE +W L A V  +  + + LEW R RVFD E+A +FL++   A    V+ V + E+ + +P ALNTVE+L+ AS  LGMGP  TM +AERLY  GY+SYPRTE+T YPE+FD++G+L  Q ++P W +  K LL EG++RPR G DAGDHPPITP+RA    E  GD  RLY+ +TRHF+A++S D ++LQT + FS   EC   F+  GK +I  G+  ++       E  LP    GD F  S + L +  T+PP YLTE+ELI  MEKHGIGTDASI  HINNI +RNYV V  GR L P  LGIVLVHGY +IDA+LVLP +R+ +E +  LIA+G+A+ Q V++H+L  F+ KF YF + I  MD L E +FSPLAATGKPLSRCGKC RFMKYI  KP RL+C  C+ETY+LPQNGTIKLYKEL+CPLDDFEL+L+S     G++GKS+PLCPYCY+HPPF  +   MGC+ C HPTC+HSL   G+    G+      G LVLD  S P WKMACN+CN +V F   N H ++  +++ CE C +A++ +++ K KSPL G ET+  GCV C  +     EL     KH
Sbjct:   38 VHEYAGSFLGQSGRFKMTSVCGHVMTLDFIGKYNNWDKVDPAELFSKAPTEKKEANPKLNMVKFLQVEGRSCDYIVLWLDCDKEGENICFEVLDATVPVMNKPRVNERSVYRAKFSSITDTDICNAMNNLGEPNHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGNLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNHEKESCLTLEWDRVRVFDREIAQMFLNITKVAKEAKVESVSRKEKAKQRPLALNTVEMLRVASAALGMGPQHTMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANNPYWAETVKALLSEGINRPRKGHDAGDHPPITPMRAASEAELGGDGWRLYEYITRHFIATVSADCKYLQTTIAFSIGPEC---FTCIGKTVISAGFTEIMPWQSIPPEENLPSCDRGDTFPISEIKLLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKANYQQVLEHTLDIFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYSLPQNGTIKLYKELRCPLDDFELVLWS----SGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPTCQHSLNMLGI----GQCVECENGVLVLDPTSGPKWKMACNKCNVIVHF-FENAHKVR-VSAETCESCDAALVDVDFNKAKSPLPGNETQHSGCVFCDPVFQDLVELKHATMKH 820