A genome-wide single-gene knockout mutant library of N. oceanica IMET1 | NanDeSyn: Nannochloropsis Design & Synthesis Initiative

The guide RNAs (gRNAs) listed in the table were validated through high-throughput sequencing, allowing for the generation of corresponding mutants based on each gRNA.

Gene locus nomenclature for N. oceanica IMET1 (IMET1v2): NO (Nannochlropsis oceanica); 01,02,03-30 (chromosome number); G (gene); 01230 (five-digit code, the first four-digit is numbered from top/north to bottom/south of chromosome, the last 0 is reserved for new locus).

Pool	Plate	Mutant ID	Gene ID	gRNA	Annotation	Mutation site	Wild type sequence	Mutation	Allele depth	Read depth	Type of variations
Pool	Plate	Mutant ID	Gene ID	gRNA	Annotation	Mutation site	Wild type sequence	Mutation	Allele depth	Read depth	Type of variations

A list of mutated strains derived from a genome-wide single-gene knockout mutant library of Nannochloropsis oceanica IMET1

The guide RNAs (gRNAs) listed in the table were validated through high-throughput sequencing, allowing for the generation of corresponding mutants based on each gRNA.